全文获取类型
收费全文 | 438篇 |
免费 | 22篇 |
国内免费 | 39篇 |
出版年
2024年 | 2篇 |
2023年 | 8篇 |
2022年 | 15篇 |
2021年 | 6篇 |
2020年 | 10篇 |
2019年 | 19篇 |
2018年 | 16篇 |
2017年 | 21篇 |
2016年 | 14篇 |
2015年 | 17篇 |
2014年 | 20篇 |
2013年 | 54篇 |
2012年 | 26篇 |
2011年 | 24篇 |
2010年 | 12篇 |
2009年 | 19篇 |
2008年 | 27篇 |
2007年 | 23篇 |
2006年 | 14篇 |
2005年 | 8篇 |
2004年 | 5篇 |
2003年 | 12篇 |
2002年 | 24篇 |
2001年 | 7篇 |
2000年 | 7篇 |
1999年 | 9篇 |
1998年 | 9篇 |
1997年 | 5篇 |
1996年 | 5篇 |
1995年 | 5篇 |
1994年 | 5篇 |
1993年 | 5篇 |
1992年 | 6篇 |
1991年 | 3篇 |
1990年 | 5篇 |
1989年 | 4篇 |
1988年 | 1篇 |
1987年 | 3篇 |
1986年 | 1篇 |
1985年 | 5篇 |
1984年 | 5篇 |
1983年 | 4篇 |
1982年 | 4篇 |
1980年 | 2篇 |
1979年 | 2篇 |
1977年 | 1篇 |
排序方式: 共有499条查询结果,搜索用时 15 毫秒
41.
Strategic mining of cyanobacterial patents from the USPTO patent database and analysis of their scope and implications 总被引:2,自引:0,他引:2
Patent analysis with the help of the strategic mining of patents from databases is important and useful within the framework
of application-oriented research and its commercialization. In the analysis reported here, we have mined cyanobacterial patents
from the patent database of the United States Patent and Trademark Office (USPTO). In order to make an assessment of the commercial
potentials of cyanobacteria, we conducted the patent search (from 1976 to April 2006) using certain generic terms and the
84 genera of cyanobacteria as keywords. The search was performed in two major ways – searching the abstracts and claims of
the patents cumulatively and searching the entire patent documents by the mode of ‘all fields’ in USPTO. In the abstract-
and claims-based search, 234 patents were obtained after the removal of overlapping patents among the keywords. An additional
31 patents were added following the ‘all fields’ search; these patents were not covered in the search that was based on abstracts
and claims. The entire package of 265 patents, of which 244 were related to cyanobacteria, was then analyzed. Information
derived from these patents identified five major areas of cyanobacterial utilization. Cyanobacteria have been patented as
a source of a wide spectrum of products, for medical, agriculture and environmental applications, for gene-based products,
for methods of cultivation and for methods of control. The chronological development in granting cyanobacterial patents was
also traced. This study demonstrates that such strategic mining and analysis of patent data can be used as an index for future
development. 相似文献
42.
Effects of nutrients and warming on Planktothrix dynamics and diversity: a palaeolimnological view based on sedimentary DNA and RNA
下载免费PDF全文
![点击此处可从《Freshwater Biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Olga Savichtcheva Didier Debroas Marie Elodie Perga Fabien Arnaud Clément Villar Emilie Lyautey Amy Kirkham Cécile Chardon Benjamin Alric Isabelle Domaizon 《Freshwater Biology》2015,60(1):31-49
43.
Production of succinic acid from sugarcane molasses supplemented with a mixture of corn steep liquor powder and peanut meal as nitrogen sources by Actinobacillus succinogenes
下载免费PDF全文
![点击此处可从《Letters in applied microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
44.
45.
Edward Málaga-Trillo Evgenia Salta Antonio FiguerasCynthia Panagiotidis Theodoros Sklaviadis 《生物化学与生物物理学报:疾病的分子基础》2011,1812(3):402-414
Transmissible spongiform encephalopathies (TSEs), otherwise known as prion disorders, are fatal diseases causing neurodegeneration in a wide range of mammalian hosts, including humans. The causative agents - prions - are thought to be composed of a rogue isoform of the endogenous prion protein (PrP). Beyond these and other basic concepts, fundamental questions in prion biology remain unanswered, such as the physiological function of PrP, the molecular mechanisms underlying prion pathogenesis, and the origin of prions. To date, the occurrence of TSEs in lower vertebrates like fish and birds has received only limited attention, despite the fact that these animals possess bona fide PrPs. Recent findings, however, have brought fish before the footlights of prion research. Fish models are beginning to provide useful insights into the roles of PrP in health and disease, as well as the potential risk of prion transmission between fish and mammals. Although still in its infancy, the use of fish models in TSE research could significantly improve our basic understanding of prion diseases, and also help anticipate risks to public health. This article is part of a Special Issue entitled Zebrafish Models of Neurological Diseases. 相似文献
46.
玉米黄粉中含有约质量分数60%的蛋白质,但很难直接应用,必须进行预处理.本文以玉米黄粉为原料,利用蛋白酶产生菌LN01进行液体发酵,分别研究了液体接种量、初始pH、培养温度和培养时间等因素对LN01菌株水解玉米黄粉蛋白的影响.实验结果表明,LN01菌株水解玉米黄粉蛋白的最佳发酵条件为:初始pH值为6.0,液体种子接种量... 相似文献
47.
The first limiting nutrients in typical laying hen diets are the sulphur-containing amino acids and, in particular, methionine. To fulfil the birds' recommended requirement, conventional diets are supplemented with synthetic methionine. As this is not allowed in organic production it becomes very important to have access to alternative high-quality protein feed ingredients. An experiment was performed to evaluate the possibility to compose a diet with 100% organically approved feed ingredients using mussel meal as a major source of methionine. The experiment included 678 Lohman Selected Leghorn (LSL) and 678 Hyline White, W-98, layers during 20 to 72 weeks of age. There were 12 aviary pens with 113 birds in each. The birds were fed one of the two experimental diets containing either 3.5% or 7% dried mussel meat meal or a commercial organic diet from a Swedish feed manufacturer for comparison. Production and mortality were recorded daily per group, and egg weight was recorded once weekly. At 33, 55 and 70 weeks, 10 eggs from each treatment group were collected and analysed for internal egg quality. Diets had no significant effect on laying percentage, egg mass, feed intake, feed conversion ratio, mortality, bird live weight or proportion misplaced, cracked or dirty eggs. Egg quality, that is, shell deformation, shell breaking strength, albumen height, shell percentage and proportion of blood and meat spots were also unaffected. There was a significant difference in egg yolk pigmentation, that is, the egg yolk was more coloured when feeding 7% mussel meal compared with the other diets. Hyline hens had lower feed intake and laying percentage, and higher egg weight, but lower egg mass production than LSL birds. The age of the birds influenced all egg quality traits except for meat and blood spots. The dry matter of the excreta was significantly lower for both genotypes fed the 7% mussel meal diet. These results indicate that mussels may be a high-quality protein source and may replace fishmeal in organic diets for layers. 相似文献
48.
Lucie Lantová Milena Svobodová Edgar Rowton Petr Volf 《Journal of invertebrate pathology》2010,105(2):182-189
Two new gregarines in the recently erected genus Psychodiella (formerly Ascogregarina), Psychodiella sergenti n. sp. and Psychodiella tobbi n. sp., are described based on morphology and life cycle observations conducted on larvae and adults of their natural hosts, the sand flies Phlebotomus sergenti and Phlebotomus tobbi, respectively. The phylogenetic analyses inferred from small subunit ribosomal DNA (SSU rDNA) sequences indicate the monophyly of newly described species with Psychodiella chagasi. Ps. sergenti n. sp. and Ps. tobbi n. sp. significantly differ from each other in the life cycle and in the size of life stages. The sexual development of Ps. sergenti n. sp. (syzygy, formation of gametocysts and oocysts) takes place exclusively in blood-fed Ph. sergenti females, while the sexual development of Ps. tobbi n. sp. takes place also in males and unfed females of Ph. tobbi. The susceptibility of Phlebotomus perniciosus, Phlebotomus papatasi, Ph. sergenti, Ph. tobbi, and Phlebotomus arabicus to both gregarines was examined by exposing 1st instar larvae to parasite oocysts. High host specificity was observed, as both gregarines were able to fully develop and complete regularly the life cycle only in their natural hosts. Both gregarines are considered as serious pathogens in laboratory-reared colonies of Old World sand flies. 相似文献
49.
A 2 × 3 factorial experiment was conducted to evaluate the effect of partial substitution of dietary fish meal with soybean meal and citric acid (CA) supplementation on growth, food utilization, muscle composition and nutrient digestibility of Beluga, Huso huso. Three isonitrogenic and isoenergetic diets, as SBM1 (soybean meal protein (SBP):fishmeal protein (FP) = 1:3), SBM2 (SBP:FP = 2:3) and SBM3 (SBP:FP = 1:1) containing two levels of CA (0 and 30 g kg−1) were fed to triplicate groups of fish for 8 weeks. The results revealed that adding CA increased (P<0.05) the weight gain (WG), specific growth rate (SGR) and protein efficiency ratio (PER) and decreased (P<0.05) food conversion rate (FCR) whereas partial substitution of fishmeal with soybean meal decreased (P<0.05) growth performance. Partial replacement of fishmeal with SBM (P=0.021) as well as CA supplementation (P=0.019) and their interaction (P<0.001) affected hepatosomatic index (HSI). No differences (P>0.05) were observed for viscerosomatic index (VSI) among treatments (P>0.05). No differences (P>0.05) were detected in moisture, protein of muscle sample among treatments, but lipid content was reduced (P<0.05) while ash content increased (P=0.035) by CA. Soybean meal decreased (P<0.05) nutrient digestibility, whereas CA improved apparent protein and phosphorus (P) digestibility (P=0.011 and P<0.001 respectively). No interaction (P<0.05) between levels of SBM and CA was found on these parameters. Results of the present study indicate that Beluga has a limited ability to utilize SBM as a protein source in practical diets whereas CA can improve growth and nutrient utilization in Beluga. 相似文献
50.
Aims: The present study was conducted to evaluate the possibility of using cyanobacterial bloom materials as a medium for white rot fungi and the capability of white rot fungi, Trichaptum abietinum 1302BG and Lopharia spadicea to biodegrade dried cyanobacterial bloom material taken from Taihu Lake. Methods and Results: The results showed T. abietinum 1302BG and L. spadicea could use the cyanobacterial bloom materials taken from Taihu Lake for growth to measure the mycelial plaque and dry‐weight mycelial pellicles of fungi. The removal rate of dried cyanobacterial bloom materials incubated with white rot fungi is approximately 100%. Conclusions: The cyanobacterial bloom material can be used as a glucose substitute in white rot fungi medium. The white rot fungi, T. abietinum 1302BG and L. spadicea, can also directly decrease the biomass of cyanobacterial bloom material taken from Taihu Lake. Significance and Impact of the Study: Cyanobacterial bloom thrives in eutrophic fresh waters all over the world. Micro‐organisms, particularly fungi, have attracted attention as possible agents for the degradation of phytoplankton species. Dealing with cyanobacterial bloom material as a medium for fungi instead of directly discharging them as organic fertilizers is a new, safe and environmentally friendly approach. 相似文献