| 蛋白酶产生菌LN01水解玉米黄粉蛋白的研究 |
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| 引用本文： | 韩秋菊,张倩倩.蛋白酶产生菌LN01水解玉米黄粉蛋白的研究[J].氨基酸和生物资源,2011,33(2):32-34. |
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| 作者姓名： | 韩秋菊 张倩倩 |
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| 作者单位： | 辽宁石油化工大学环境与工程学院,辽宁抚顺,113001;辽宁石油化工大学环境与工程学院,辽宁抚顺,113001 |
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| 摘 要： | 玉米黄粉中含有约质量分数60%的蛋白质,但很难直接应用,必须进行预处理.本文以玉米黄粉为原料,利用蛋白酶产生菌LN01进行液体发酵,分别研究了液体接种量、初始pH、培养温度和培养时间等因素对LN01菌株水解玉米黄粉蛋白的影响.实验结果表明,LN01菌株水解玉米黄粉蛋白的最佳发酵条件为:初始pH值为6.0,液体种子接种量...
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| 关 键 词： | 蛋白酶 玉米黄粉蛋白 酶活力 |
Study on Corn Gluten Meal Hydrolyzed by Proteinase Producing Strain LN01 |
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| HAN Qiu-ju.Study on Corn Gluten Meal Hydrolyzed by Proteinase Producing Strain LN01[J].Amino Acids & Biotic Resources,2011,33(2):32-34. |
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| Authors: | HAN Qiu-ju |
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| Institution: | HAN Qiu-ju(Environmental and Biological Engineering faculty,Liaoning University of Petroleum and Chemical Technology,Fushun113001,China) |
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| Abstract: | There is sixty percent of protein in the gluten meal.But,it is hard to be used directly before pretreatment.To determine the optimum conditions of hydrolyzing corn gluten meal,we studied the effects of inoculum quantity,pH,and temperature and culture time on producing proteinase by LN01 strain.The results indicated that the optimum conditions of producing the enzyme were as follows： culture temperature of 25℃,pH of 6.0,inoculum quantity of 12% and culture time of 3 days. |
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| Keywords: | proteinase corn gluten meal enzyme activity |
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