马立克氏病病毒强毒GA株基因组BamH Ⅰ-L片段的序列分析

马立克氏病病毒（Ｍａｒｅｋ’ｓｄｉｓｅａｓｅｖｉｒｕｓ,ＭＤＶ）是一种能诱导鸡淋巴组织增生或淋巴肿瘤的细胞结合性疱疹病毒,由此而引起的马立克氏病（Ｍａｒｅｋ’ｓｄｉｓｅａｓｅ,ＭＤ）也是迄今为止唯一可以利用疫苗进行有效控制的肿瘤性疾病。鉴于此,作为研...     

刺槐上分离的花生矮化病毒的研究

刺槐花叶病是我国北方刺槐（Ｒｏｂｉｎｉａｐｓｅｕｄｏａｃａｃｉａ）常见病害。１９８７～１９９５年对河南、河北、山东和北京部分地区刺槐进行调查,刺槐花叶病发病率为４％～８７．５％。该病害主要特征是：叶片出现浅绿与绿色相间带有疱疹的花叶症状,叶缘波状扭...     

247例人乳头瘤病毒的PCR检测结果分析

２４７例生殖系分泌物标本用ＰＣＲ检测人乳头瘤病毒ＨＰＶ－ＤＮＡ。结果显示：阳性感染率达８７．６％,在与临床疾病密切的亚型中,６、１１型感染率（６０％）,高于１６、１８型感染率（５１．６％）,但统计学处理没有显著性差异。女性发病率明显高于男性。易感人群组以２０～４０岁最高（占感染人数的８４．４％）,并且有年轻化的趋势（１０～２０岁年龄组占８．４％）。     

流行性感冒灭活疫苗的研制

将流感病毒接种于鸡胚尿囊腔培养,收集鸡胚尿囊液及羊水,经甲醛灭活后,采用蔗糖速率区带超离心的方法进行提纯经脱糖、紫外照射后,按照《ＷＨＯ生物制品规程》的要求,试制出含有Ａ１型、Ａ３型和Ｂ型流感病毒的流行性感冒Ⅲ价灭活疫苗     

传染性法氏囊病病毒在次代鸡胚成纤维细胞上的增殖

研究了用次代鸡胚成纤维细胞（SCEF）增殖传染性法氏囊病病毒（IBDV）的可能性。在研究了原代鸡胚成纤维细胞（PCEF）与SCEF的生长特性的基础上,就各种培养方式采用PCEF与SCEF增殖IBDV进行了比较。结果表明,可用SCEF代替PCEF进行IBDV的增殖培养。     

肝细胞靶向性脂质体介导的硫代磷酸反义寡核苷酸在体内外抑制乙型肝炎病毒的研究

在体外细胞培养系统和裸鼠动物体内,观察了人肝细胞靶向性脂质体介导的部分硫代型和全部硫代型反义寡核苷酸对乙型肝炎病毒的抑制作用。结果显示,针对HBVe基因翻译起始区的18聚体的硫代磷酸反义寡核苷酸在体内、外对HBV DNA、HBV mRNA及HBsAg、HBeAg均有不同程度的抑制作用。研究说明,反义寡核苷酸是抗HBV有效的基因治疗候选药物。     

森林脑炎病毒分子生物学研究进展

森林脑炎(TBE)病毒属黄病毒科,基因姐RNA含有单个开放阅读框架,5′端编码病毒的结构蛋白,3′端编码非结构蛋白。翻译成聚蛋白后,通过细胞和病毒编码的蛋白酶裂解产生单个的病毒蛋白。成熟的病毒是由两个相关的E和M膜蛋白脂质包膜所包围的立体对称的核衣壳组成。包膜E蛋白在病毒的感染周期中对细胞的识别和穿入细胞具有极其重要的功能,同时E蛋白诱导保护性的免疫反应,E蛋白内某一位点单个氨基酸的改变可引起病毒毒力的改变。因此,对TBE病毒分子生物学的研究有助于了解病毒与宿主细胞相互作用的机理,为病毒感染的特异性诊断、疫苗的研制和抗病毒药物的设计提供理论依据。     

Prevalence of human foamy virus-related sequences in the Korean population

The possible association of human foamy virus (HFV) with human thyroid disorders such as Graves' disease (GD) has been a topic of controversy due to the inconsistent results reported by several groups of investigators. Here we report the investigation of the presence of HFV-related sequences in the Korean population. DNA was obtained from peripheral blood lymphocytes from 24 GD patients and 23 healthy blood donors and subjected to PCR amplification using three sets of nested primers derived fromgag, env, and LTR regions of the HFV genome. Contrary to previously reported studies, our analysis identified HFV-related sequences in the genomes of both healthy individuals and the GD patients. However, the nature of the HFV genome present in each group appeared to be different. We detected all 3 regions of HFV-related sequences in 29% of the HFV-positive GD patients, while no samples in the control group amplified all three regions. This suggests that the LTR may be used as a tool for screening for HFV in GD patients. Our data favor the hypothesis of a relationship between GD and the presence of HFV-related sequences, though in a complex way.     

Patterns of circulating hepatitis B surface antigen variants among vaccinated children born to hepatitis B surface antigen carrier and non-carrier mothers

Hepatitis B virus (HBV) variants that possessed missense mutation within the neutralization epitope of the major S antigen as defined by amino acid residues (aa#) 124–147, termed the a determinant variants, were identified through a population-based serosurvey of 2,305 children of the vaccinated birth cohorts born after 1986. Data on the 678 nucleotides encoding the S antigen of HBV were available for 75 HBV strains that were collected from 63 vaccinated children and 12 unvaccinated or incompletely vaccinated children, and 21 HBV strains from 25 unvaccinated adults. Among the diverse patterns of one to three amino acid substitutions within the a determinant, 145-Arg occurred most frequently (5/14); other variants were: 126-Ala, 127-Thr, 126-Ser/131-Asn/133-Thr, 129-His, 129-Arg, 123-Asn/131-Ile, 133-Leu, 141-Glu, and 141-Arg/144-Ala. Only one of these variants occurred in the 16 hepatitis B surface antigen (HBsAg)-carrier children born to HBsAg-negative mothers, whereas 12 of these variants occurred in the 20 (50%) children born to HBsAg-positive mothers. In addition, early administration of HBV vaccine within the noenatal period increased the likelihood of the emergence of these variants to 64.7% (11/17). Five of the 21 (23.8%) unvaccinated HBsAg-carrier adults harbored the a determinant variants possessing mutations within aa# 125–136, i.e. the putative first loop formed by the cysteine disulfide bonds. Vaccinated children were likely to harbor HBV variants possessing mutations involving altered charge of side chains and/or its hydrophobicity of amino acid residues within the putative second loop between aa#140 and 146. Our data suggest that emergence of these HBV S gene mutants in the phase of HBV vaccination program would be most common among populations in whom perinatal/vertical transmission of HBV is most common, i.e. southeast Asian and the Taiwanese.     

Expression of HIV-1nefin yeast causes membrane perturbation and release of the myristylated Nef protein

The human immunodeficiency virus type 1 (HIV-1) Nef protein is essential for AIDS pathogenesis, but its function remains highly controversial. During stresses such as growth in the presence of copper or at elevated temperature, myristylated Nef is released from yeast cells and, after extended culture in stationary phase, it accumulates in the supernatant as a dense membranous material that can be centrifuged into a discrete layer above the cell pellet. This material is unique to Nef-producing cells and represents a convenient source of Nef that may have application in further biological studies. Within the yeast cell, electron microscopic examination shows that Nef localises in novel, membrane-bound bodies. These data support the evidence for a role of Nef in membrane perturbation and suggest that there may be a similar localisation for myristylated Nef in HIV-1 infected cells.