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21.
We evaluated the performance of Coptera haywardi (Ogloblin) (Diapriidae) and Pachycrepoideus vindemiae (Rondani) (Pteromalidae), both hymenopteran pupal parasitoids of Anastrepha spp. (Diptera: Tephritidae). Performance was studied by manipulating the following environmental conditions in the laboratory: (1) soil type, (2) soil moisture content, (3) soil compaction, and (4) depth at which pupae were buried in the soil. There were two experiments: in the first, exposure time of pupae was held constant and in the second, it varied. In the first experiment, C. haywardi was significantly more effective than P. vindemiae in parasitizing fly pupae. With exposure time held constant (36 h), only soil type and pupal burial depth were significantly related to parasitism rates. While P. vindemiae only parasitized pupae located on the soil surface, C. haywardi attacked pupae that were buried up to 5 cm deep, performing better in clayey than in loamy soil. In the second experiment, exposure time (24, 36, 48, and 72 h) had no significant effect on parasitism rates, but soil type did. P. vindemiae again only attacked pupae on the soil surface while C. haywardi was also able to parasitize pupae that were buried up to 5 cm deep. We conclude that C. haywardi represents a viable candidate to replace the environmentally unfriendly P. vindemiae in augmentative biological control programs against fruit flies. 相似文献
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Abstract. 1. Most of what is known about parasitoid behaviour comes from laboratory observations: field quantitative observations on searching parasitoids are extremely difficult to do and are rare. The basic components of Aphytis melinus 's response to California red scale ( Aonidiella aurantii ) were studied in the laboratory: encounter, rejection, drumming, probing, oviposition, and host-feeding. It was then asked whether these observations provided a reliable guide to behaviour in the field in a situation that was very different from the laboratory.
2. Field observations were carried out on bark on the trunk and interior branches of trees where live scale density is extremely high in patches, dead scale make up 90% of all scale, and could be expected to interfere with Aphytis search.
3. The laboratory observations predicted well the time taken in the field for each basic event (drumming or probing) and average times spent on a scale. Also well predicted were the distributions of times spent on drumming, probing, and total time on a scale. Rejection rates were much higher in the field. Thus, the laboratory studies predicted foraging behaviour in the field with variable success; potential explanations for observed mismatch between laboratory and field and its possible larger implications are discussed. 相似文献
2. Field observations were carried out on bark on the trunk and interior branches of trees where live scale density is extremely high in patches, dead scale make up 90% of all scale, and could be expected to interfere with Aphytis search.
3. The laboratory observations predicted well the time taken in the field for each basic event (drumming or probing) and average times spent on a scale. Also well predicted were the distributions of times spent on drumming, probing, and total time on a scale. Rejection rates were much higher in the field. Thus, the laboratory studies predicted foraging behaviour in the field with variable success; potential explanations for observed mismatch between laboratory and field and its possible larger implications are discussed. 相似文献
24.
EFFECT OF MEDIA ON GROWTH AND INTERACTIONS BETWEEN A RANGE OF SOIL-BORNE GLASSHOUSE PATHOGENS AND ANTAGONISTIC FUNGI 总被引:10,自引:1,他引:9
JOHN M. WHIPPS 《The New phytologist》1987,107(1):127-142
25.
In vitro pathogenicity tests demonstrated that Hirschmanniella caudacrena is pathogenic to Ceratophyllum demersum (coontail). Symptoms were chlorotic tissue, deformed stems, and, finally, death of the plant. Inoculum densities of 500 nematodes per 5-cm-long cutting in a test tube containing 50 ml of water resulted in death and decay of some of the cuttings within 8 weeks; 100 nematodes killed the plants in 12 weeks, and 50 and 25 nematodes killed them in 16 weeks. The lowest inoculum level of 10 nematodes did not seriously affect the plants at 16 weeks when the experiment was terminated. A second test conducted outdoors in glass jars containing 3 liters of water and two cuttings weighing a total of 15 g fresh weight showed damage, but results were not statistically significant. Hydrilla verticillata inoculated with H. caudacrena was not affected seriously. 相似文献
26.
Michael A. Keller 《Entomologia Experimentalis et Applicata》1987,43(1):55-59
Walking speeds of female Trichogramma exiguum Pinto & Platner were fastest on maize and soybean (12 cm/min), intermediate on tomato (8 cm/min), and slowest on woolly mullein, Verbascum thapsus (3 cm/min). Similarly, rates of turning along the paths of walking T. exiguum were smallest on maize (median angle=0°±15°), intermediate on soybean and tomato, and greatest on V. thapsus (median angle=30°±15°). Leaf trichome density and morphology influenced walking behavior. Walking was slowed and flight initiation delayed for T. exiguum walking on Amaranthus hybridus leaves compared to either maize or filter paper. When inundative releases are conducted, the effects of plant surfaces on searching rates and arrestment should be considered in determining release rates of Trichogramma spp.
Résumé La vitesse de marche a été déterminée en lâchant des individus sur chaque substrat végétal et en traçant leur parcours sur une plaque de verre placée à 9 mm au dessus du sujet. Les déviations angulaires de portions successives des tracés, longues de 1 mm, ont été utilisées pour mesurer les taux de changement de direction. Dans une deuxième expérience, des individus ont été lâchés au milieu d'une cercle de 40 mm de diamètre sur chaque substrat et les temps écoulés avant l'envol ou pour atteindre le bord du cercle à la marche ont servi à évaluer respectivement la propension au vol et la vitesse de déambulation.Les vitesses de marche ont été plus rapides sur maïs et soja (12 cm/min), moyennes sur tomate (8 cm/min) et les plus lentes sur Verbascum thapsus (Scrophulariaceae) (3 cm/min). De le même façon, les angles de changement de direction au cours des marches effectuées par T. exiguum ont été plus petits sur maïs, moyens sur soja et tomate, et plus grands sur V. thapsus. Chez T. exiguum marchant sur des feuilles d'Amaranthus hybridus L. (Amaranthaceae), la marche a été plus lente et l'envol plus tardif que sur maîs ou papier filtre.Lors de lâchers inondatifs, les effets des surfaces végétales sur les vitesses d'exploration et d'arrêt devraient être pris en compte pour déterminer les vitesses de lâcher des Trichogramma spp.相似文献
27.
P. H. J. F. van den Boogert H. Reinartz K. A. Sjollema M. Veenhuis 《Antonie van Leeuwenhoek》1989,56(2):161-174
The mycoparasitic interactions of Verticillium biguttatum with Rhizoctonia solani and with a variety of other soil-borne fungi were investigated in dual cultures. V. biguttatum interacted with various soil fungi by appressed growth along the host hyphae and infrequent penetrations. Intracellular growth and subsequent sporulation, however, only occurred with R. solani, a few binucleate Rhizoctonia and Ceratobasidium spp., and Sclerotinia sclerotiorum. Effective mycoparasitism on sclerotia was restricted to those belonging to R. solani.Electron-microscopic observations revealed that V. biguttatum can penetrate the host cell with infection tubes. This process is probably mediated by enzymatic hydrolysis of the cell wall. Subsequently, trophic hyphae develop within the host cytoplasm, ultimately resulting in death of the host cell. 相似文献
28.
Sulfonylurea herbicides are potent inhibitors of plant growth and are extremely active against a wide spectrum of weeds. They are used at very low rates (10–50 g ai/ha) and cause rapid inhibition of root and shoot growth of young plants. Routine chemical assays for detecting low levels of these compounds are difficult and there is need to develop sensitive bioassay methods for detecting their extremely low residue levels in the soil.This paper describes a simple pot bioassay method with a self watering system using turnip (Brassica rapa) seedlings as test plants for quantitative determination of sulfonylurea herbicides. Results are presented with six of these compounds whose activity was investigated in widely differing substrates. The potential availability to plants was calculated from the dose-response curves in different substrates. The dose-response relationship has been described by a specifically developed computer model. Details are also given of a direct seeded bioassay method with controlled watering system using several test species for detection of sulfonylurea herbicides. The potential uses and practical applications of both techniques are discussed. 相似文献
29.
Biological sensors are becoming more important to monitor the quality of the aquatic environment. In this paper the valve movement response of freshwater (Dreissena polymorpha) and marine (Mytilus edulis) mussels is presented as a tool in monitoring studies. Examples of various methods for data storage and data treatment are presented, elucidating easier operation and lower detection limits. Several applications are mentioned, including an early warning system based on this valve movement response of mussels. 相似文献
30.
本文用实验揭示了瞳孔对光动态反应具有采样控制特性。实验中采用各种不同时间间隔的双脉冲光,以开环的方式(Maxwellian View)刺激瞳孔,当双脉冲之间间隔较长时,瞳孔反应相当于对双脉冲光的两次脉冲分别产生瞬态收缩;当双脉冲时间间隔短于0.6s 时,其反应就成了一次瞬态收缩,与单个光脉冲所引起的瞳孔反应一样。同—受试者的多次实验结果相同,不同受试者所得结果也基本一致。故瞳孔对脉冲刺激光引起反应后,必须至少约隔0.6s 才能对另一次脉冲光产生反应,这就说明了瞳孔动态反应具有离散的采样控制特性。实验还进一步证明,瞳孔系统的控制机制是双重模式的控制:不同的刺激条件下,瞳孔反应可呈现为瞬态反应(AC)或持续反应(DC),瞬态反应的 AC 通道为离散的采样控制,持续反应的 DC 通道为连续控制。 相似文献