全文获取类型
收费全文 | 3901篇 |
免费 | 300篇 |
国内免费 | 274篇 |
出版年
2024年 | 7篇 |
2023年 | 159篇 |
2022年 | 142篇 |
2021年 | 271篇 |
2020年 | 184篇 |
2019年 | 219篇 |
2018年 | 191篇 |
2017年 | 143篇 |
2016年 | 157篇 |
2015年 | 216篇 |
2014年 | 231篇 |
2013年 | 305篇 |
2012年 | 185篇 |
2011年 | 168篇 |
2010年 | 151篇 |
2009年 | 191篇 |
2008年 | 187篇 |
2007年 | 192篇 |
2006年 | 176篇 |
2005年 | 136篇 |
2004年 | 125篇 |
2003年 | 102篇 |
2002年 | 78篇 |
2001年 | 51篇 |
2000年 | 39篇 |
1999年 | 31篇 |
1998年 | 31篇 |
1997年 | 35篇 |
1996年 | 50篇 |
1995年 | 27篇 |
1994年 | 19篇 |
1993年 | 22篇 |
1992年 | 28篇 |
1991年 | 21篇 |
1990年 | 14篇 |
1989年 | 14篇 |
1988年 | 13篇 |
1985年 | 11篇 |
1984年 | 14篇 |
1983年 | 8篇 |
1982年 | 20篇 |
1981年 | 11篇 |
1980年 | 7篇 |
1979年 | 12篇 |
1978年 | 14篇 |
1977年 | 10篇 |
1976年 | 11篇 |
1975年 | 14篇 |
1974年 | 9篇 |
1973年 | 10篇 |
排序方式: 共有4475条查询结果,搜索用时 93 毫秒
91.
Nan Zhang Jiyun Yang Anfei Fang Jiyang Wang Dayong Li Yuejiao Li Shanzhi Wang Fuhao Cui Junjie Yu Yongfeng Liu You-Liang Peng Wenxian Sun 《Molecular Plant Pathology》2020,21(4):445-459
The biotrophic fungal pathogen Ustilaginoidea virens causes rice false smut, a newly emerging plant disease that has become epidemic worldwide in recent years. The U. virens genome encodes many putative effector proteins that, based on the study of other pathosystems, could play an essential role in fungal virulence. However, few studies have been reported on virulence functions of individual U. virens effectors. Here, we report our identification and characterization of the secreted cysteine-rich protein SCRE1, which is an essential virulence effector in U. virens. When SCRE1 was heterologously expressed in Magnaporthe oryzae, the protein was secreted and translocated into plant cells during infection. SCRE1 suppresses the immunity-associated hypersensitive response in the nonhost plant Nicotiana benthamiana. Induced expression of SCRE1 in rice also inhibits pattern-triggered immunity and enhances disease susceptibility to rice bacterial and fungal pathogens. The immunosuppressive activity is localized to a small peptide region that contains an important ‘cysteine-proline-alanine-arginine-serine’ motif. Furthermore, the scre1 knockout mutant generated using the CRISPR/Cas9 system is attenuated in U. virens virulence to rice, which is greatly complemented by the full-length SCRE1 gene. Collectively, this study indicates that the effector SCRE1 is able to inhibit host immunity and is required for full virulence of U. virens. 相似文献
92.
Qi Li Ji Wang Tian Bai Ming Zhang Yuling Jia Danyu Shen Meixiang Zhang Daolong Dou 《Molecular Plant Pathology》2020,21(4):502-511
EDS1 (Enhanced Disease Susceptibility 1) plays a crucial role in both effector-triggered immunity activation and plant basal defence. However, whether pathogen effectors can target EDS1 or an EDS1-related pathway to manipulate immunity is rarely reported. In this study, we identified a Phytophthora capsici Avirulence Homolog (Avh) RxLR (Arg-any amino acid-Leu-Arg) effector PcAvh103 that interacts with EDS1. We demonstrated that PcAvh103 can facilitate P. capsici infection and is required for pathogen virulence. Furthermore, genetic evidence showed that PcAvh103 contributes to virulence through targeting EDS1. Finally, PcAvh103 specifically interacts with the lipase domain of EDS1 and can promote the disassociation of EDS1–PAD4 (Phytoalexin Deficient 4) complex in planta. Together, our results revealed that the P. capsici RxLR effector PcAvh103 targets host EDS1 to suppress plant immunity, probably through disrupting the EDS1–PAD4 immune signalling pathway. 相似文献
93.
Carina Mikolai Nadine Kommerein Alexandra Ingendoh‐Tsakmakidis Andreas Winkel Christine S. Falk Meike Stiesch 《Cellular microbiology》2020,22(8)
The host‐microbe relationship is pivotal for oral health as well as for peri‐implant diseases. Peri‐implant mucosa and commensal biofilm play important roles in the maintenance of host‐microbe homeostasis, but little is known about how they interact. We have therefore investigated the early host‐microbe interaction between commensal multispecies biofilm (Streptococcus oralis, Actinomyces naeslundii, Veillonella dispar, Porphyromonas gingivalis) and organotypic peri‐implant mucosa using our three‐dimensional model. After 24 hr, biofilms induced weak inflammatory reaction in the peri‐implant mucosa by upregulation of five genes related to immune response and increased secretion of IL‐6 and CCL20. Biofilm volume was reduced which might be explained by secretion of β‐Defensins‐1, ‐2, and CCL20. The specific tissue reaction without intrinsic overreaction might contribute to intact mucosa. Thus, a relationship similar to homeostasis and oral health was established within the first 24 hr. In contrast, the mucosa was damaged and the bacterial distribution was altered after 48 hr. These were accompanied by an enhanced immune response with upregulation of additional inflammatory‐related genes and increased cytokine secretion. Thus, the homeostasis‐like relationship was disrupted. Such profound knowledge of the host‐microbe interaction at the peri‐implant site may provide the basis to improve strategies for prevention and therapy of peri‐implant diseases. 相似文献
94.
Dan Wang Li Tian Dan-Dan Zhang Jian Song Shuang-Shuang Song Chun-Mei Yin Lei Zhou Yan Liu Bao-Li Wang Zhi-Qiang Kong Steven J. Klosterman Jun-Jiao Li Jie Wang Ting-Gang Li Sabiu Adamu Krishna V. Subbarao Jie-Yin Chen Xiao-Feng Dai 《Molecular Plant Pathology》2020,21(5):667-685
Secreted small cysteine-rich proteins (SCPs) play a critical role in modulating host immunity in plant–pathogen interactions. Bioinformatic analyses showed that the fungal pathogen Verticillium dahliae encodes more than 100 VdSCPs, but their roles in host–pathogen interactions have not been fully characterized. Transient expression of 123 VdSCP-encoding genes in Nicotiana benthamiana identified three candidate genes involved in host–pathogen interactions. The expression of these three proteins, VdSCP27, VdSCP113, and VdSCP126, in N. benthamiana resulted in cell death accompanied by a reactive oxygen species burst, callose deposition, and induction of defence genes. The three VdSCPs mainly localized to the periphery of the cell. BAK1 and SOBIR1 (associated with receptor-like protein) were required for the immunity triggered by these three VdSCPs in N. benthamiana. Site-directed mutagenesis showed that cysteine residues that form disulphide bonds are essential for the functioning of VdSCP126, but not VdSCP27 and VdSCP113. VdSCP27, VdSCP113, and VdSCP126 individually are not essential for V. dahliae infection of N. benthamiana and Gossypium hirsutum, although there was a significant reduction of virulence on N. benthamiana and G. hirsutum when inoculated with the VdSCP27/VdSCP126 double deletion strain. These results illustrate that the SCPs play a critical role in the V. dahliae–plant interaction via an intrinsic virulence function and suppress immunity following infection. 相似文献
95.
96.
97.
Alexander N. Prutkov Andrey V. Matveev Lyubov E. Grebenkina Mikhail G. Akimov Yulia V. Berezovskaya 《Nucleosides, nucleotides & nucleic acids》2020,39(7):943-963
AbstractSome 5-substituted ribavirin analogues have a high antiviral and anticancer activity, but their mechanisms of action are obviously not the same as their parent compound. The SAR studies performed on 3 (5)-substituted 1,2,4-triazole nucleosides have shown a high dependency between the structure of the 3 (5)-substituent and the level of antiviral/anticancer activity. The most active substances of the row contain coplanar with the 1,2,4-triazole ring aromatic substituent which is connected by a rigid ethynyl bond. However, the compounds with the trans-vinyl linker also had antiviral activity. We decided to study the antitumor activity of ribavirin analogues with alkyl/aryl vinyl substituents in the 5th position of the 1,2,4-triazole ring. Protected nucleoside analogues with various 5-alkylvinyl substituents were obtained by Horner-Wadsworth-Emmons reaction from the common precursor and converted to the nucleosides. Arylvinyl nucleosides were synthesised according the reported procedures. All compounds did not show significant antiproliferative activity on several tumour cell lines. Coplanar aromatic motif in the 5-substituent for the anticancer activity manifestation was confirmed. 相似文献
98.
99.
100.
《Microbes and infection / Institut Pasteur》2020,22(9):416-422
Human immunodeficiency virus (HIV) is the causative agent of acquired immunodeficiency syndrome (AIDS). Novel strategies to combat this pandemic include the discovery of cellular proteins targeting distinct steps of the HIV replication cycle. Here, we summarize our current knowledge on antiviral proteins interfering with the infectivity of released HIV particles. 相似文献