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31.
NEW MURID RODENTS FROM THE LATE CENOZOIC OF YUSHE BASIN, SHANXI 总被引:8,自引:8,他引:0
Lawrence J.Flynn 《古脊椎动物学报》1992,(1)
<正> As scientific collaborators of the Chinese-American joint project "Neogene Rocks and Faunas, Yushe Basin, Shanxi, PRC", the present authors, with William R. Downs, Northern Arizona University, sampled the Yushe microfauna in the fall of 1987 and 1988. The fossil temains were retrieved by surface collection and by wet-sieving bulk quantities of sediment. 相似文献
32.
Summary A re-examination of earlier NPK fertilization experiments in Douglas fir stands on sandy soils shows the effects of high nitrogen
input by air pollution during the last 10–15 years on plant nutrition at these sites. In 1960, experimental plots showed a
positive growth reaction to nitrogen, phosphorus, and potassium fertilization. All suffered from severe phosphorus deficiency
in 1984, low phosphorus in the needles was invariably accompanied by a high nitrogen content, with all N/P ratios between
20 and 30. The same conclusion emerges from an independent investigation of nutrient status of a selection of Douglas fir
stands. Hence, if stand productivity and a balanced nutrient status of the trees is to be maintained, the increase in atmospheric
input of nitrogen calls for supplementary fertilization. Given the current N/P ratios in the needles, a positive growth response
to phosphorus fertilization is to be expected. 相似文献
33.
34.
When monocytes isolated from human blood adhere to glass substratum, actin- and vinculin-containing punctate plaques rapidly appear at the ventral surface of the cells. We show here that highly purified human leukocyte interferon (IFN) can inhibit formation of these adhesion plaques in a dose-dependent manner. Complete inhibition was obtained when 300 IU/ml IFN were added into the cell-seeding medium. Plaques already formed in the absence of IFN were only partially affected by subsequent addition of IFN into the culture medium. Prevention by IFN of the formation of the adhesion plaques was associated with loosened attachment of the cells to the substratum. Effect of IFN on cellular morphology was complex. At higher doses, IFN added to the cultures within 24 h of seeding almost completely inhibited the differentiation of monocytes to macrophages and most of the cells remained rounded. At lower doses, however, an enhancement of the bipolar spreading was seen and the end result was a culture with predominantly elongated fibroblastoid cells. The latter cells, unlike the fibroblastoid cells in untreated monocyte-macrophage cultures, were completely devoid of the actin plaques, while the reorganization of vimentin-type intermediate filaments took place in a normal manner. These results further support the view that the actin- and vinculin-containing plaques have a role in mediating firm adherence of human monocytes to growth substratum. 相似文献
35.
C L Wood M S O'Dorisio L M Vassalo W B Malarkey T M O'Dorisio 《Regulatory peptides》1985,12(3):237-248
The vasoactive intestinal polypeptide (VIP) receptor was characterized on the GH3 rat pituitary tumor cell line using competitive binding studies with peptides having sequence homology with VIP. Further studies investigated receptor coupling to the adenylate cyclase complex by measurement of cAMP levels. Finally, the molecular weight of the receptor was estimated by affinity labeling techniques. Studies using 125I-VIP and unlabeled competing peptides revealed a single class of high affinity binding sites with a dissociation constant (KD) of 17 +/- 2 nM (mean +/- S.E.M.) for VIP, 275 +/- 46 nM for peptide histidine isoleucine (PHI), and 1380 +/- 800 nM for human pancreatic growth hormone releasing factor (GHRF). VIP and PHI each stimulated intracellular cAMP accumulation in a dose-dependent manner; both peptides demonstrated synergism with forskolin. In contrast, GHRF neither stimulated accumulation of cAMP nor demonstrated synergism with forskolin. VIP plus PHI (1 microM each) caused no significant increase in cAMP over either VIP or PHI alone, implying that the two peptides act through the same receptor. Covalent crosslinking of 125I-VIP to its binding site using either disuccinimidyl suberate (DSS) or ethylene glycol bis(succinimidyl succinate) (EGS) was followed by SDS-PAGE and autoradiography. The result is consistent with an Mr 47 000 VIP-binding subunit comprising or being associated with the VIP receptor of GH3 pituitary tumor cells. 相似文献
36.
元江干热河谷山地五百年来植被变迁探讨 总被引:15,自引:2,他引:13
元江河谷是云南省最干热地区之一,在海拔800—900米以下的山地上广泛分布着稀树灌草丛。根据《元江府志》(1714年编纂)、《元江州志》(1826年编纂)、《元江志稿》(1922年编篡)及对现存植被的考察,本文探讨了元江干热河谷山地五百年来植被的变迁。 元江县森林复盖率的减少与人口的增加有密切关系,十七世纪中期以前,森林复盖率在75%以上,十八、十九世纪时为70%左右,1958年为61.5%,1975年为27.3%,至1982年则为19.3%。研究表明,在十九世纪以前,这个地区分布的主要植被是热带季雨林,甚而热带季节雨林,以后热带稀树灌草丛则迅速发展。植被的历史变化与土壤流失密切相关。植物群落的演变是由以乔木树种为优势演变为以灌木种类为优势,再演变为以多年生草木植物为优势,而最后则成为裸地。本文也讨论了这个地区植被恢复的方法。 相似文献
37.
Multiple discriminant analysis is a useful multivariate technique in vegetation studies that can be employed for several purposes, even if the underlying statistical assumptions are not satisfied. An example of application of this method is discussed: multiple discriminant analysis was successfully used for evaluating the predictivity of forest types defined by numerical classification of vegetation data with respect to soil variables.Species nomenclature follows Pignatti (1982) for vascular plants and Augier (1966) for mosses.The research has been supported by the IDROSER (Bologna, Italy) and by the Italian C. N. R. (Grant no. 83.02548.04, resp. Prof. A. Pirola). We wish to thank Prof. E. Feoli, Prof. E. van der Maarel, Prof. L. Orlóci and Prof. A. Pirola for suggestions. We are also indebted to Dr N. Filippi who analyzed the soil samples. 相似文献
38.
Trehalose dimycolate, a glycolipid component of the cell walls of mycobacteria, induces interstitial pneumonitis and alveolar hemorrhages in C57BL/6 and C57BL/10 mice. Homozygous nude (nu/nu) mice of these backgrounds are not susceptible to this form of pulmonary injury. However, after administration of T-lymphocyte-enriched spleen cell preparations from syngeneic donors, homozygous nude mice become susceptible to trehalose dimycolate. The observations suggest that production of pulmonary lesions by this mycobacterial component is dependent on T lymphocytes. While the mechanisms are still under study, we propose that trehalose dimycolate can function as an activator of T lymphocytes and that products of activated T cells are responsible for production of the pulmonary lesions. 相似文献
39.
Murine peritoneal macrophages (M phi), activated in vivo or in vitro, remarkably inhibited the uptake of thymidine by a lens epithelial cell line, while resident M phi, or M phi induced by thioglycollate, exhibited much lower or no cytostatic capacity. The target cells were partially protected from the cytostatic activity by the anti-inflammatory agents indomethacin, aspirin, and dexamethasone, but not by lipoxygenase inhibitors. The protective activity of indomethacin and aspirin, but not of dexamethasone, was completely counteracted by prostaglandin E2 (PGE2). Yet, PGE2 alone has no effect on the uptake of [3H]thymidine by lens epithelial cells. PGE1 resembled PGE2 in its effect on this system, whereas PGA2, PGB2, or PGF2 alpha had no detectable activity. The counteracting effect of PGE2 was mimicked by dibutyryl cAMP or by cholera toxin, an agent which increases cAMP levels. These findings suggest that PGEs are not direct cytostatic agents, but rather, are essential mediators for the development of the cytostasis. Activated M phi did not lyse cells of the original lens epithelial cell line, but caused substantial cytolysis of cells of a subline derived from it. In contrast to its aforementioned effect on the cytostasis, PGE2 inhibited the cytolytic activity of M phi. Thus, this study provides a first demonstration in a single system of the opposite effects of PGEs on M phi activity on target cells, i.e., mediating the cytostasis and inhibiting the cytolysis. 相似文献
40.
Effects of glucagon on the redox states of cytochromes in mitochondria in situ in perfused rat liver 总被引:1,自引:0,他引:1
S Kimura T Suzaki S Kobayashi K Abe E Ogata 《Biochemical and biophysical research communications》1984,119(1):212-219
The effects of glucagon on the respiratory function of mitochondria in situ were investigated in isolated perfused rat liver. Glucagon at the concentrations higher than 20 pM and cyclic AMP (75 microM) stimulated hepatic respiration, and shifted the redox state of pyridine nucleotide (NADH/NAD) in mitochondria in situ to a more reduced state as judged by organ fluorometry and beta-hydroxybutyrate/acetoacetate ratio. The organ spectrophotometric study revealed that glucagon and cyclic AMP induced the reduction of redox states of cytochromes a(a3), b and c+c1. Atractyloside (4 micrograms/ml) abolished the effects of glucagon on these parameters and gluconeogenesis from lactate. These observations suggest that glucagon increases the availability of substrates for mitochondrial respiration, and this alteration in mitochondrial function is crucial in enhancing gluconeogenesis. 相似文献