肾衰康浓缩丸质量标准研究

目的:建立肾衰康浓缩丸质量控制方法.方法:采用TLC鉴别肾衰康浓缩丸中大黄、丹参;采用HPLC-ELSD对制剂中黄芪甲苷进行含量测定,流动相为乙腈-水(30:70),流速:1 mL/min,柱温:28℃,漂移管温度:42℃,氮气:355kpa.结果:大黄、丹参的TLC鉴别斑点清晰、专属性强;黄芪甲苷在1.0μg-10.0μg之间呈良好的线性关系(r=0.9997,n=5),平均回收率为99.81%,RSD为1.73%(n=6).结论:所建立的方法简便准确、专属性强、重现性好,可作为控制肾衰康浓缩丸质量的方法.     

右归丸对鼠膝骨性关节炎的治疗作用及Wnt信号通路相关因子表达的影响

目的：分析右归丸对膝骨性关节炎（KOA）大鼠Wnt信号通路相关因子表达的影响,探讨右归丸对KOA大鼠的保护机制。方法：SPF级SD大鼠60只,按照体重法随机分为假手术组、模型组、硫酸氨基葡萄糖组、右归丸高、中、低剂量组（n=10）。采用改良Hulth法复制膝骨关节炎大鼠模型。右归丸高中低剂量组分别按20、10、5 g/kg灌服相应的药物,硫酸氨基葡萄糖组按0.17 g/kg灌服硫酸氨基葡萄糖,假手术组和模型组灌服等体积的生理盐水,干预8周。末次给药后摘取膝关节,通过膝关节病理切片观察各组大鼠软骨组织病理改变;采用RT-PCR法对各组大鼠软骨组织DKK1、WISP1、Wnt1、β-catenin和LRP5 mRNA的表达水平进行对比分析;通过Western blot法检测各组大鼠软骨组织DKK1、WISP1、Wnt1、LRP5和β-catenin的蛋白质含量的变化。结果：与假手术组比较,模型组大鼠关节软骨受损严重,Mankin评分明显升高（P<0.05）;DKK1 mRNA表达水平和蛋白质表达水平明显降低（P<0.05）;WISP1、Wnt1、β-catenin、LRP5 mRNA表达水平及蛋白质表达水平明显升高（P<0.05）。与模型组比较,右归丸高剂量组和硫酸氨基葡萄糖组关节软骨病变明显减轻;Mankin评分明显减轻（P<0.05）;大鼠软骨组织中DKK1 mRNA表达水平和蛋白表达水平明显升高,WISP1、Wnt1、β-catenin、LRP5 mRNA及蛋白表达水平显著降低（P<0.05）。结论：右归丸通过抑制Wnt信号通路中WISP1、Wnt1、β-catenin、LRP5的表达,促进DKK1细胞因子的表达,发挥对KOA的保护作用。     

王氏保赤丸治疗小儿便秘疗效观察

目的:观察王氏保赤丸治疗小儿便秘的疗效,探讨其有效机制。方法:将60例便秘患儿,年龄1岁至6岁,随机分为治疗组31例,平均年龄(3.00±0.30)岁,对照组29例,平均年龄(3.00±0.30)岁。治疗组采用王氏保赤丸配合便秘饮食及摄取膳食纤维,增加饮水,训练规律排便治疗;对照组采用便秘饮食和摄取膳食纤维,增加饮水,训练规律排便治疗。结果:治疗组总有效率达90.8%,明显高于对照组。结论:王氏保赤丸治疗小儿便秘具有显著的疗效。其机制可能是通过对脾胃的双向调节作用所致。     

麝香保心丸与曲美他嗪联合治疗老年冠心病心绞痛的疗效及对血浆BNP水平的影响

目的:分析麝香保心丸联合曲美他嗪治疗老年冠心病心绞痛的疗效及对血浆B型脑钠肽(BNP)水平的影响。方法:选取2010年1月-2015年1月我院收治的老年冠心病心绞痛患者128例作为研究对象,采用随机数字表法将其分为实验组(64例)和对照组(64例),单纯使用曲美他嗪对对照组患者进行治疗,联合使用麝香保心丸和曲美他嗪对实验组患者进行治疗,观察两组患者的临床疗效,比较治疗前后两组血浆BNP水平、左心室舒张末期内径(LVEDD)、左心室收缩末期内径(LVESD)以及左室射血分数(LVEF),统计两组的不良反应。结果:实验组的治疗总有效率为92.19%,明显高于对照组的62.50%,差异具有统计学意义(P0.05);治疗后,实验组与对照组血浆BNP、LVEDD、LVESD、LVEF均得到改善,但是实验组改善更明显,差异具有统计学意义(P0.05);两组不良反应率比较,无统计学差异(P0.05)。结论:应用麝香保心丸联合曲美他嗪治疗老年冠心病心绞痛的临床疗效显著,且明显降低血浆BNP水平,值得在临床上推广应用。     

右归丸与液体钙片对老年骨质疏松症患者血清雌激素和骨钙素水平的影响

目的:研究右归丸联合液体钙片对老年骨质疏松症患者血清雌激素水平、骨钙素(BGP)水平及临床疗效的影响。方法:选取2014年10月至2015年9月本院收治的82例老年骨质疏松症患者,根据入院顺序分为观察组和对照组,每组41例。对照组使用骨化三醇软胶囊和液体钙软胶囊进行治疗,观察组在此基础上加以右归丸进行治疗。比较两组患者治疗前和治疗1个月后血清雌二醇(E2)水平、BGP水平、骨密度变化,分析两组患者的临床疗效。结果:治疗后,观察组的血清E2水平显著高于对照组(P0.05),BGP水平显著低于对照组(P0.05),腰椎L1-4正位、右侧股骨颈、左侧股骨颈骨密度显著高于对照组(p0.05),总临床疗效率显著高于对照组(P0.05)。结论:右归丸联合液体钙片治疗老年骨质疏松症患者能明显改善患者血清雌激素、BGP水平,增加骨密度,临床疗效良好。     

白凤丸及其主要活性成分对机体及胃肠道上皮细胞离子转运功能的影响

中药是我们祖国的宝藏,有着两千多年的历史。它具有维护机体健康、预防、缓解以及治疗疾病等作用。虽然白凤丸被用来治疗妇科疾患已有很久历史,但它目前也被作为天然保健品而广泛使用。越来越多的研究表明白凤丸对机体多系统、多器官功能具有调节作用。本文对白凤丸及其主要活性成分对机体功能的调节,尤其是对胃肠道上皮细胞离子转运功能的影响以及作用机制进行综述。     

A proprietary herbal drug Young Yum Pill ameliorates chronic fatigue syndrome in mice

BackgroundChronic fatigue syndrome (CFS) is a complex disease with few effective and safe therapies. Young Yum Pill (YYP), a proprietary herbal drug, has been used to relieve CFS-like symptoms. The pharmacological basis of this application of YYP is unknown.PurposeThis study aimed to investigate the pharmacological effects and mechanisms of action of YYP in a mouse model of CFS.Study design and methodsA food restriction and exhaustive swimming-induced mouse CFS model was used to evaluate the effects of YYP. Lymphocyte proliferation was assessed by MTT assays. T-lymphocyte subsets were analyzed by flow cytometry. Serum biochemical parameters were determined using commercial kits. Protein levels were measured by immunoblotting.ResultsIntragastric administration of YYP (2.85, 5.70, 11.40 g/kg) daily for 21 consecutive days significantly prolonged swimming time and diminished body weight loss of CFS mice. Mechanistic investigations revealed that YYP increased thymus and spleen indices of CFS mice, enhanced proliferation of lipopolysaccharide- or concanavalin A-stimulated spleen lymphocytes, and increased CD3⁺CD4⁺ and CD3⁺CD8⁺ T-cells in the spleen. YYP increased glycogen content in gastrocnemius muscle and liver, and lowered levels of triglyceride, lactic acid and urea nitrogen in sera of CFS mice. YYP suppressed the elevation of serum level of malondialdehyde, the increase of activities of lactic dehydrogenase and creatine phosphokinase, and the decrease of activity of the serum antioxidant enzyme superoxide dismutase in CFS mice. Moreover, YYP upregulated protein level of activated AMPK in gastrocnemius muscle and liver of CFS mice.ConclusionsYYP ameliorates CFS by reversing metabolic changes, reducing oxidative damage, and improving some immune function parameters in mice. This study provides pharmacological justifications for the use of YYP in treating fatigue, including CFS.     

Effective suppression of ovarian cyclicity in the lowland gorilla with an oral contraceptive

To determine the effects of a human oral contraceptive on normal and abnormal reproductive endocrine patterns in two lowland gorillas (Gorilla gorilla), daily urine samples were analyzed by radioimmunoassay for estrone conjugates (EC) and pregnanediol-3-glucuronide (PdG). During a pre-treatment period one female (F1) demonstrated regular menstrual cycles averaging 27.6 ± 1.8 days, whereas ovarian cyclicity in female 2 (F2) was consistently prolonged, ranging from 37–51 days. A 56 day regimen of an oral contraceptive (Demulen 50®) was administered to both females beginning in the late luteal phase, and within 6 and 7 days of treatment onset (F1 and F2, respectively) urinary EC and PdG declined to and remained at concentrations consistent with amenorrhea throughout the administration period. Ten and twenty days after contraceptive withdrawal (F1 and F2, respectively) an EC peak was observed with subsequent PdG elevations 1–2 days later. Mean cycle length and luteal phase durations were not different (P > 0.05) before or after treatment for either female, while combined peak PdG concentrations were greater (P < 0.05) for the first 3 months after treatment compared to pre-treatment values. These results indicate that a human oral contraceptive rapidly suppresses ovarian activity in female lowland gorillas, but that the abnormal endocrine pattern observed in F2 could not be redirected into a normal profile after contraceptive withdrawal. © 1992 Wiley-Liss Inc.