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101.
Hypericum perforatum cv. Anthos) is presented. Isotope tracer experiments were performed on plantlets regenerated from thidiazuron-induced stem
explants and grown on MS basal medium for 2 months. Radiolabel from 14C-tryptophan was recovered as 14C-indoleacetic acid, 14C-tryptamine, 14C-5-hydroxytryptophan, 14C-serotonin and 14C-melatonin in the treated St. John's wort plantlets. Chromatographic peak identity was confirmed by high performance liquid
chromatography-mass spectrometry-mass spectrometry and quantification of melatonin by radioimmunoassay. Significantly more
radiolabel was recovered in serotonin relative to melatonin under low light conditions with this ratio being reversed under
increased lighting, indicating that the rate of flow through this biosynthetic pathway is regulated, at least in part, by
light.
Received: 9 November 1999 / Revision received: 16 December 1999 / Accepted: 21 December 1999 相似文献
102.
分光光度法测定豆类及其粗蛋白质中的色氨酸 总被引:8,自引:0,他引:8
建立一种快速、准确测定各种豆类试样及粗蛋白质中的色氨酸含量的分光光度法讨论最佳试验条件及试剂用量,反应物在410nm处具有最大吸收波长ε=1.103×104L·mol-1·cm-1,回收率93.50-110.20%,相对标准偏差RSD<2.61. 相似文献
103.
《Bioorganic & medicinal chemistry letters》2014,24(15):3329-3332
Mass-guided fractionation of the MeOH extract from a specimen of the Australian marine sponge Hyrtios sp. resulted in the isolation of two new tryptophan alkaloids, 6-oxofascaplysin (2), and secofascaplysic acid (3), in addition to the known metabolites fascaplysin (1) and reticulatate (4). The structures of all molecules were determined following NMR and MS data analysis. Structural ambiguities in 2 were addressed through comparison of experimental and DFT-generated theoretical NMR spectral values. Compounds 1–4 were evaluated for their cytotoxicity against a prostate cancer cell line (LNCaP) and were shown to display IC50 values ranging from 0.54 to 44.9 μM. 相似文献
104.
Gerhard Haspel Michael Hunger Robert Schmucker Wolfgang Hillen 《Molecular & general genetics : MGG》1990,220(3):475-477
Summary The trpE gene from Acinetobacter calcoaceticus encoding the anthranilate synthase component I was cloned, identified by deletion analysis and sequenced. It encodes a predicted polypeptide of 497 amino acids with a calculated molecular weight of 55323. Its primary structure shows 49% identical amino acids with the enzyme from Clostridium thermocellum, 45% with that of Thermus thermophilus and only 35% with that of Escherichia coli. The codon usage of the trpE genes encoding the most homologous enzymes differs greatly indicating selection for amino acid maintainance. The homologies are clustered in the C-terminal 200 amino acids of the sequences indicating that this part is important for enzymic activity. 相似文献
105.
K. Peng A. J. W. G. Visser A. van Hoek C. J. A. M. Wolfs M. A. Hemminga 《European biophysics journal : EBJ》1990,18(5):285-293
The subnanosecond fluorescence and motional dynamics of the tryptophan residue in the bacteriophage M13 coat protein incorporated within pure dioleoylphosphatidylcholine (DOPC) as well as dioleoylphosphatidylcholine/dioleoylphosphatidylglycerol (DOPC/DOPG) and dimyristoylphosphatidylcholine/dimyristoylphosphatidylglycerol (DMPC/DMPG) bilayers (80/20 w/w) with various L/P ratio have been investigated. The fluorescence decay is decomposed into four components with lifetimes of about 0.5, 2.0, 4.5 and 10.0 ns, respectively. In pure DOPC and DOPC/DOPG lipid bilayers, above the phase transition temperature, the rotational diffusion of the protein molecules contributes to the depolarization and the anisotropy of tryptophan is fitted to a dual exponential function. The longer correlation time, describing the rotational diffusion of the whole protein, shortens with increasing temperature and decreasing protein aggregation number. In DMPC/DMPG lipid bilayers, below the phase transition, the rotational diffusion of the protein is slowed down such that the subnanosecond anisotropy decay of tryptophan in this system reflects only the segmental motion of the tryptophan residue. Because of a heterogeneous microenvironment, the anisotropy decay must be described by three exponentials with a constant term, containing a negative coefficient and a negative decay time constant. From such a decay, the tryptophan residue within the aggregate undergoes a more restricted motion than the one exposed to the lipids. At 20 degrees C, the order parameter of the transition moment of the isolated tryptophan is about 0.9 and that for the exposed one is about 0.5. 相似文献
106.
Monitoring the Effect of a Tryptophan Load on Brain Indole Metabolism in Freely Moving Rats by Simultaneous Cerebrospinal Fluid Sampling and Brain Dialysis 总被引:18,自引:8,他引:10
P. H. Hutson G. S. Sarna B. D. Kantamaneni G. Curzon 《Journal of neurochemistry》1985,44(4):1266-1273
Rats were given L-tryptophan, 50 mg/kg i.p., and its concentration in the CNS was monitored in individual freely moving animals using repeated sampling of cisternal CSF and concurrent striatal dialysis. The 5-hydroxytryptamine metabolite 5-hydroxyindoleacetic acid (5-HIAA) was also measured. Results were compared with changes of central tryptophan and 5-HIAA concentrations in brains of rats killed at various times after administration of L-tryptophan, 50 mg/kg i.p. Tryptophan changes in CSF were proportionate to those in whole brain and followed essentially identical time courses. Results for the striatal dialysate and whole striatum also paralleled each other. Similarly, results for 5-HIAA showed proportionality between CSF and brain and between dialysate and striatum. The data obtained were used to determine pharmacokinetic data for individual rats, i.e., areas under curves for both tryptophan and 5-HIAA and half-lives for the decline of tryptophan. Kinetic parameters varied considerably from rat to rat. However, mean half-lives for tryptophan in CSF, brain, dialysate, and striatum were all comparable. Results in general show the value of repeated CSF sampling and intracerebral dialysis for concurrent monitoring of changes of indole metabolism in the whole brain and a specific brain region, respectively. The methods should be suitable for the continuous monitoring of changes of central transmitter metabolism in parallel with observation of behavior following environmental or dietary changes or drug administration. They also should be of use in the investigation of drug kinetics in the CNS. 相似文献
107.
Siu-Leung Lee Minocher Reporter Sharon N. Hsu James L. Corbin Gregory Luli Paul L. Burkhouse John H. Litchfield 《Journal of industrial microbiology & biotechnology》1988,3(5):293-297
Summary A cell line, NA13-2, was selected as a rapidly growing colony of protoplasts from a UV(254 nm)-fluorescent cell line, NA13-1, which originated from a tryptamine-resistant strain ofCatharanthus roseus NA13. Cell line NA13-2 lost the capability to produce indole alkaloids. Tryptophan fed to these cells was converted toN
b-acetyltryptamine as the major product. The free acetyl coenzyme A content of NA13-2 cells was 50% higher than in the mother cells. The total lipid content of the NA13-2 cells was 2.5-fold that in the NA13 cells. In spite of the similarity in the fatty acid content to that of the mother cell line NA13, the total lipid extract of NA13-2 cells appeared as a wax instead of an oil, resulting from the presence of sterol esters.This paper was presented in part at the Annual Meeting of the Society for Industrial Microbiology, Boston, MA, 1985, and the International Congress of the Plant Tissue Culture Association, Minneapolis, MN, 1986. 相似文献
108.
Sequence of Two mRNAs Encoding Active Rat Tryptophan Hydroxylase 总被引:16,自引:9,他引:7
Michèle C. Darmon Bernard Guibert Vincent Leviel Mireille Ehret Michel Maitre Jacques Mallet 《Journal of neurochemistry》1988,51(1):312-316
Two full-length cDNA clones that encode functional rat tryptophan hydroxylase (EC 1.14.16.4), the key enzyme in serotonin synthesis, have been isolated from a rat pineal gland library. These two clones correspond to the 1.8- and 4-kilobase mRNA species, respectively. They contain the same coding sequence corresponding to a 51,010-dalton protein and differ in the length of their 3' untranslated regions. 相似文献
109.
Presence of Kynurenic Acid in the Mammalian Brain 总被引:14,自引:6,他引:8
Kynurenic acid, a tryptophan metabolite able to antagonize the actions of the excitatory amino acids, has been identified and measured for the first time in the brain of mice, rats, guinea pigs, and humans by using an HPLC method. Its content was 5.8 +/- 0.9 in mouse brain, 17.8 +/- 2.0 in rat brain, 16.2 +/- 1.5 in guinea pig brain, 26.8 +/- 2.9 in rabbit brain, and 150 +/- 30 in human cortex (pmol/g wet wt. mean +/- SE). The regional distribution of this molecule was uneven. In rats, guinea pigs, and rabbits, the brainstem was the area richest in this compound. Tryptophan administration (100-300 mg/kg, i.p.) to rats resulted in a significant increase of the brain content of kynurenic acid. Similarly, 1 h after probenecid administration (200 mg/kg, i.p.), the brain content of kynurenate increased by fourfold, thus suggesting that its turnover rate is relatively fast. 相似文献
110.