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91.
Hwang DY Cho JS Chae KR Kang TS Hwang JH Lim CH Lee SH Lim HJ Min SH Sheen YY Jang IS Kim YK 《Archives of biochemistry and biophysics》2003,415(2):137-145
Differential expression of the tetracycline-controlled transactivator (tTA)-driven human cytochrome p450 (CYP) 1B1 gene was found in the livers of male mice, at high levels in neonates, but at low levels in adults. The goals of this study were to determine whether the differential expression of the tTA-driven human CYP1B1 (hCYP1B1) gene in neonates and adults was testosterone dependent and whether flutamide, a representative potent antiandrogen, led to the induction of hCYP1B1. This was tested by treating castrated transgenic mice with testosterone propionate and musk extracts. It was concluded that: (i). the levels of expression of both tTA and hCYP1B1 gradually declined, with clear changes being apparent between 2 and 4 weeks of age, (ii). castration of adult males resulted in the increased expressions of both tTA and hCYP1B1 to levels similar to those found in adult females, (iii). treatment of castrated male and adult female mice with testosterone propionate and musk extracts led to the restoration of the levels of expression of hCYP1B1 in the adult males, and (iv). treatment of adult males with flutamide caused an increase in the levels of expression of hCYP1B1 in the adult females, as indicated by the antiandrogenic activity. Thus, the differential expression of the tTA-driven hCYP1B1 gene in the transgenic mice was caused by androgen, and it is possible that castrated male and adult female mice expressing the tTA-controlled hCYP1B1 could be used as the basis for a strategy for the detection of androgens and antiandrogens. 相似文献
92.
Valdés R Reyes B Alvarez T García J Montero JA Figueroa A Gómez L Padilla S Geada D Abrahantes MC Dorta L Fernández D Mendoza O Ramirez N Rodriguez M Pujol M Borroto C Brito J 《Biochemical and biophysical research communications》2003,310(3):742-747
This paper provides an evaluation of a plant-derived HBsAg-specific antibody in the immunopurification of the recombinant HBsAg for vaccine purposes. This plant-derived antibody was obtained from different batches of 100-200kg of tobacco leaves and coupled to Sepharose CL-4B with high efficiency. The plant-derived antibody immunoaffinity matrix purification behavior (elution capacity, antigen purity, purification cycles, and ligand leakage) was comparable to that of its mouse-derived monoclonal antibody homolog. This result supports the feasibility of using this plant-derived antibody for the immunopurification of the Hepatitis B surface antigen for human use, opening a new possibility to overcome the constrain of monoclonal antibody production in mice. 相似文献
93.
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95.
Kawasaki M Fujino M Li XK Kitazawa Y Funeshima N Takahashi R Ueda M Amano T Hakamata Y Kobayashi E 《Biochemical and biophysical research communications》2003,311(4):920-928
Suicide gene expression in specific tissue of transgenic animals has been used for cell-specific ablation. To examine the influence of hepatocyte removal, we produced the herpes simplex virus thymidine kinase (HSVtk) transgenic rat, whose gene was regulated by an albumin enhancer promoter. The liver presence of HSVtk was demonstrated in one line of the transgenic rats. We injected ganciclovir (GCV, 50mg/kg) into the rat on alternate days. After 28 days of GCV administration, liver tissues, and blood of the rats were collected. The histological investigation revealed infiltration of T cells, macrophages, granulocytes/neutrophils, and hepatocyte cell death. The biochemistry analysis demonstrated elevated levels of AST, ALT, and total bilirubin in transgenic rat. In conclusion, the transgenic rat with expressed albumin-specific HSVtk developed experimental hepatitis with administration of GCV, and will be a useful model to facilitate the evaluation of drug effects for clinical control of liver disease. 相似文献
96.
97.
Promoter strength and tissue specificity effects on growth of tomato plants transformed with maize sucrose-phosphate synthase 总被引:10,自引:0,他引:10
Laporte MM Galagan JA Prasch AL Vanderveer PJ Hanson DT Shewmaker CK Sharkey TD 《Planta》2001,212(5-6):817-822
When sucrose-phosphate synthase (SPS; EC 2.4.1.14) is expressed in tomato (Lycopersicon esculentum Mill.) from a ribulose-1,5-bisphosphate carboxylase-oxygenase (Rubisco) small subunit (rbcS) promoter, yields are often unchanged but when SPS is expressed from a Cauliflower Mosaic Virus 35S promoter, yield is enhanced
up to 80%. Two explanations for this phenomenon are (i) that expression of SPS in tissues other than leaves accounts for the
increased yield or (ii) that the lower level of expression directed by the 35S promoter is more beneficial than the high level
of expression directed by the rbcS promoter. To test the first hypothesis, we conducted a reciprocal graft experiment, which showed that root SPS activity did
not substantially affect growth. To test the second hypothesis, we conducted a field trial using a backcrossed, segregating,
population of SPS-transformed plants derived from 35S and rbcS lines. The optimal dose of SPS activity for growth was approximately twice that of the wild type regardless of which promoter
was used. The effect of SPS on growth was the result of a shift in partitioning of carbon among starch, sucrose, and ionic
compounds (primarily amino acids), rather than of an increase in net photosynthesis. Excessive SPS activity resulted in a
decreased rate of amino acid synthesis, which could explain the non-linear response of plant growth to the level of SPS expression.
Received: 23 May 2000 / Accepted: 24 July 2000 相似文献
98.
Lawson E.C. Weiss J.D. Thomas P.E. Kaniewski W.K. 《Molecular breeding : new strategies in plant improvement》2001,7(1):1-12
Potato leafroll poleovirus and the Colorado potato beetle (Leptinotarsa decemlineata (Say)) are major pests of potato in the USA. The US Department of Agriculture estimates that over 50% of annual insecticide use on potato is applied to control the Colorado potato beetle and aphids that transmit potato leafroll virus (PLRV). To address this issue, Russet Burbank potatoes have been genetically modified for a high level of resistance to infection and the resulting disease symptoms caused by PLRV and to feeding damage caused by the Colorado potato beetle. This resistance was achieved by the expression of the unmodified full-length replicase gene of PLRV and the cry3A insect control protein gene from Bacillus thuringiensis var. tenebrionis. Plant expression constructs containing various modifications of the PLRV replicase gene were produced during the development of this product. The genes in these constructs were a full-length unmodified replicase (open reading frame 2a/2b), an antisense orientation of the full-length cDNA, an open reading frame 1 translation of the full-length gene, and a gene truncation containing the 3 sense coding portion of the replicase gene. Growth chamber experiments demonstrated that transformation of plants with the full-length and 3 sense coding constructs substantially protected these potato plants from infection and disease symptoms caused by PLRV. The Russet Burbank potato expressing the full-length PLV replicase gene and the cry3A gene is a new potato product from NatureMark called NewLeaf Plus®. 相似文献
99.
Summary. Plants are able to synthesise all amino acids essential for human and animal nutrition. Because the concentrations of some
of these dietary constituents, especially methionine, lysine, and threonine, are often low in edible plant sources, research
is being performed to understand the physiological, biochemical, and molecular mechanisms that contribute to their transport,
synthesis and accumulation in plants. This knowledge can be used to develop strategies allowing a manipulation of crop plants,
eventually improving their nutritional quality.
This article is intended to serve two purposes. The first is to provide a brief review on the physiology of methionine synthesis
in higher plants. The second is to highlight some recent findings linked to the metabolism of methionine in plants due to
its regulatory influence on the aspartate pathway and its implication in plant growth. This information can be used to develop
strategies to improve methionine content of plants and to provide crops with a higher nutritional value.
Received January 28, 2000 Accepted March 3, 2000 相似文献