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1.
Yeon‐Sook Kim Yeon‐Joo Choi Kyung‐Min Lee Kyu‐Joong Ahn Heung‐Chul Kim Terry Klein Ju Jiang Allen Richards Kyung‐Hee Park Won‐Jong Jang 《Microbiology and immunology》2017,61(7):258-263
2.
Methylglyoxal‐induced apoptosis is dependent on the suppression of c‐FLIPL expression via down‐regulation of p65 in endothelial cells 下载免费PDF全文
3.
Mary T. Doan Michael D. Neinast Erika L. Varner Kenneth C. Bedi David Bartee Helen Jiang Sophie Trefely Peining Xu Jay P. Singh Cholsoon Jang J. Eduardo Rame Donita C. Brady Jordan L. Meier Kenneth B. Marguiles Zoltan Arany Nathaniel W. Snyder 《Journal of lipid research》2022,63(6):100224
Anabolic metabolism of carbon in mammals is mediated via the one- and two-carbon carriers S-adenosyl methionine and acetyl-coenzyme A. In contrast, anabolic metabolism of three-carbon units via propionate has not been shown to extensively occur. Mammals are primarily thought to oxidize the three-carbon short chain fatty acid propionate by shunting propionyl-CoA to succinyl-CoA for entry into the TCA cycle. Here, we found that this may not be absolute as, in mammals, one nonoxidative fate of propionyl-CoA is to condense to two three-carbon units into a six-carbon trans-2-methyl-2-pentenoyl-CoA (2M2PE-CoA). We confirmed this reaction pathway using purified protein extracts provided limited substrates and verified the product via LC-MS using a synthetic standard. In whole-body in vivo stable isotope tracing following infusion of 13C-labeled valine at steady state, 2M2PE-CoA was found to form via propionyl-CoA in multiple murine tissues, including heart, kidney, and to a lesser degree, in brown adipose tissue, liver, and tibialis anterior muscle. Using ex vivo isotope tracing, we found that 2M2PE-CoA also formed in human myocardial tissue incubated with propionate to a limited extent. While the complete enzymology of this pathway remains to be elucidated, these results confirm the in vivo existence of at least one anabolic three- to six-carbon reaction conserved in humans and mice that utilizes propionate. 相似文献
4.
K. H. Jang J. W. Seo K. B. Song C. H. Kim S. K. Rhee 《Bioprocess and biosystems engineering》1999,21(5):453-458
Secretion of levansucrase from Zymomonas mobilis in Escherichiacoli by glycine supplement was investigated. A significant amount of levansucrase (about 25% of total activity) was found in intact whole-cells. Cell fractionation experiments showed that levansucrase was found both in the periplasmic space and in the cytoplasmic fraction of E. coli. None or only trace amounts of levansucrase was detected in the extracellular culture broth at 24 h of cultivation and it accrued with the increasing concentration of glycine in the culture medium and duration of the culture period. Optimal glycine concentration for the maximum secretion of levansucrase was in the range of 0.8-1%, in which approximately 20-50% of levansucrase was released into the extracellular fraction at 24 h of cultivation, although glycine retarded the bacterial growth. 相似文献
5.
J. S. Cho B. K. Jang S. M. Lee I. J. Lee C. H. Lee 《Plant biology (Stuttgart, Germany)》2020,22(3):514-521
- Information on the optimal conditions to promote the germination of Lamprocapnos spectabilis (L.) Fukuhara seeds is limited; consequently, this study was conducted to establish the requirements to break seed dormancy and promote germination.
- The selected seeds had morphophysiological dormancy and had not begun embryo development. To study the dormancy breaking and embryo development processes, seeds were subjected to constant or changing temperature treatments during moist stratification.
- High temperature and humidity resulted in vigorous embryo growth, with the longest embryos occurring after 1 month of incubation at 20 °C. At 4 °C, the seeds required incubation period of at least 3 months to germinate. Embryo growth and germination were higher with changing high and low temperatures than under a constant temperature, and changing temperatures also considerably changed the endogenous hormone levels, embryo development and germination. Bioactive gibberellin (GA) content was higher in seeds incubated at 20 °C for 1 month, then at 4 °C for 2 months. The content of endogenous abscisic acid in seeds subjected to the same treatment decreased by 97.6% compared with that of the untreated seeds.
- Embryo growth and seed germination require changing high and low temperatures; however, exogenous GA3 could substitute for high temperatures, as it also causes accelerated germination. In this study, the seeds of L. spectabilis were identified as an intermediate simple type, a sub‐level of morphophysiologically dormant seeds.
6.
Endothelial STAT3 Activation Increases Vascular Leakage Through Downregulating Tight Junction Proteins: Implications for Diabetic Retinopathy 下载免费PDF全文
7.
Seunghun Lee Won Yeong Park Hoonchul Chang Bumju Kim Won Hyuk Jang Seonghan Kim Younghoon Shin Myoung Joon Kim Kyung Hwa Lee Eui Hyun Kim Euiheon Chung Ki Hean Kim 《Journal of biophotonics》2020,13(1)
Delineation of brain tumor margins during surgery is critical to maximize tumor removal while preserving normal brain tissue to obtain optimal clinical outcomes. Although various imaging methods have been developed, they have limitations to be used in clinical practice. We developed a high‐speed cellular imaging method by using clinically compatible moxifloxacin and confocal microscopy for sensitive brain tumor detection and delineation. Moxifloxacin is a Food and Drug Administration (FDA) approved antibiotic and was used as a cell labeling agent through topical administration. Its strong fluorescence at short visible excitation wavelengths allowed video‐rate cellular imaging. Moxifloxacin‐based confocal microscopy (MBCM) was characterized in normal mouse brain specimens and visualized their cytoarchitecture clearly. Then, MBCM was applied to both brain tumor murine models and two malignant human brain tumors of glioblastoma and metastatic cancer. MBCM detected tumors in all the specimens by visualizing dense and irregular cell distributions, and tumor margins were easily delineated based on the cytoarchitecture. An image analysis method was developed for automated detection and delineation. MBCM demonstrated sensitive delineation of brain tumors through cytoarchitecture visualization and would have potentials for human applications, such as a surgery‐guiding method for tumor removal. 相似文献
8.
Jae-Il Ahn In-Keun Jang Young-Kwon Seo Hee-Hoon Yoon Yeon-Ho Shin Jae-Chan Kim Kye-Yong Song Eun-Kyung Yang Jung-Keug Park 《Biotechnology and Bioprocess Engineering》2008,13(3):279-287
Herein, we reconstructed a rabbit corneal epithelium on a lyophilized amniotic membrane (LAM) using a modified version of
two Teflon rings (the Ahn’s supporter). We compared the corneal epithelial cells we had differentiated in vitro using air-liquid interface (6 days, 12 days) and submerged (6 days, 12 days) cultures and followed a six-day tilting dynamic
air-liquid interface culture with a six-day tilting submerged culture. We characterized the reconstructed corneal epithelium
using digital photography, histological imaging, and transmission electron microscopy. The reconstructed corneal epithelium
created under air-liquid interface culture exhibited a healthier basal corneal epithelial layer than that created under submerged
culture. The reconstructed corneal epithelium on the LAM that was produced using the tilting dymanic culture exhibited a healthy
basal layer. We therefore proposed that tilting submerged culture not only supplied nutrients from the medium to the corneal
epithelial cells on the LAM, but it also removed the horny layer in the upper part of the reconstructed corneal epithelium,
presumably by mimicking the effects of blinking. This study demonstrated that corneal epithelium reconstruction on a LAM using
a tilting submerged culture after a tilting air-liquid interface culture may be useful not only for allogeneic or autologous
transplantation, but also for in vitro toxicological test kits. 相似文献
9.
Kyoung-Soon Jang Woo-Jae Chung Hyun-Ki Kim Yun-Gon Kim Yoon-Sik Lee Byung-Gee Kim 《Biotechnology and Bioprocess Engineering》2008,13(4):445-452
A novel α-Gal resin was chemo-enzymatically synthesized for the efficient adsorption of anti-α-Gal antibodies in human serum
for xenotransplantation. To covalently conjugate a hexanoate linker with lactose and N-acetylglucosamine, both acceptor sugars were acetylated and brominated. Then, α-and β-galactoses were sequentially added
to the linker-containing saccharides at their non-reducing ends by using recombinant α-(1,3)-and β-(1,4)-galactosyltransferases
from E. coli. Finally, the synthesized α-Gal derivatives were immobilized on HiCore, a core-shell type resin, that was functionalized with amino groups on the shell region, as a packing material on-column.
Using this method we were able to demonstrate that the α-Gal HiCore resin had a reduced level of non-specific protein adsorption compared with the commercially available polystyrene supports,
TentaGel, and agarose-based supports, when Lectin BS-I was used as the model binding protein. Furthermore, the α-Gal HiCore resin was more efficient at eliminating anti-α-Gal IgGs from the total human IgGs through immunoadsorption than the other
two α-Gal resins, α-Gal TentaGel and α-Gal agarose. The α-Gal HiCore resin developed in this study can be utilized in a wide range of applications including ex vivo immunoadsorption and as a quantitative assay of anti-Gal antibody in human sera. 相似文献
10.