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31.
An artificial osmotic cell: a model system for simulating osmotic processes and for studying phenomena of negative pressure in plants 总被引:3,自引:1,他引:2
Abstract An artificial osmotic cell has been constructed using reverse osmosis membranes. The cell consisted of a thin film of an osmotic solution (thickness: 100 to 200 μm) containing a non-permeating solute and was bounded between the membrane and the front plate of a pressure transducer which continuously recorded cell turgor. The membrane was supported by metal grids to withstand positive and negative pressures (P). At maximum, negative pressures of up to –0.7 MPa (absolute) could be created within the film on short-term and pressures of up to –0.3 MPa could be maintained without cavitation for several hours. As with living plant cells, the application of osmotic solutions of a non-permeating solute resulted in monophasic relaxations of turgor pressure from which the hydraulic conductivity of the membrane (Lp) and the elastic modulus of the cell (?) could be estimated. The application of solutions with permeating solutes resulted in biphasic pressure relaxation curves (as for living cells) from which the permeability (Ps) and reflection (σs) coefficients could be evaluated for the given membrane. Lp, Ps, and σs were independent of P and did not change upon transition from the positive to the negative range of pressure. It is concluded that the artificial cell could be used to simulate certain transport properties of living cells and to study phenomena of negative pressure as they occur in the xylem and, perhaps, also in living cells of higher plants. 相似文献
32.
J. A. BUNCE 《Plant, cell & environment》1988,11(3):205-208
Abstract. Gas exchange measurements were made on single leaves of three C3 and one C4 species at air speeds of 0.4 and 4.0 m s−1 to determine if boundary layer conductance substantially affected the substomatal pressure of carbon dioxide. Boundary layer conductances to water vapour were 0.4 to 0.5 mol m−2 s−1 at the lower air speed, and 1.2 to 1.5 mol m−2 s−1 at the higher air speed. Substomatal carbon dioxide pressures were about 5 Pa lower at low boundary layer conductance in the C3 species, and about 3 Pa lower in the C4 species when measurements were made at high and moderate photosynthetic photon flux densities. No evidence of stomatal adjustment to altered boundary layer conductance was found. Photosynthetic rates at high photon flux densities were reduced by about 20% at the low air speed in the C3 species. The commonly reported values of substomatal carbon dioxide pressure for C3 and C4 species were found to occur only when measurements were made at the higher air speed. 相似文献
33.
In vitro enzyme activities of glycolysis, pentose-phosphate pathway and dark CO2 fixation were assayed in batch cultures of heterotrophic Catharanthus roseus cells under various gassing rates and partial pressures of carbon dioxide. Detrimental effects of low pCO2 culture conditions on the growth characteristics could be linked to marked changes in levels of enzymes of primary metabolism during growth. The enzyme levels observed during the early stages of growth were found to be more stable when a constant pCO2 (20 mbar) was maintained and enabled exponential growth to be reached more rapidly.The importance of carbon dioxide as a conditioning factor of the culture medium is discussed. 相似文献
34.
Joseph B. Long Alberto Martinez-Arizala Donald D. Rigamonti John W. Holaday 《Peptides》1988,9(6):1335-1344
Intrathecal (IT) injection of arginine vasopressin (AVP) in rats caused a transient (<30 min), dose-related paralysis of the hindlimbs, loss of hindlimb and tail nociceptive responsiveness, and increased mean arterial pressure. Motor dysfunction was produced with comparable potency by lysine vasopressin (LVP) and arginine vasotocin (AVT); oxytocin (OXY) was approximately 1000 times less potent. Paralysis induced by these peptides was selectively blocked following IT pretreatment with 0.5 nmoles of the vasopressin V1 receptor antagonist [1-(β-mercapto-β,β-cyclopentamethylene propioinic acid), 2-(O-methyl)tyrosine] Arg8-vasopressin (d(CH2)5[Tyr(Me2)]AVP). Pressor and antinociceptive responses to AVP were also blocked by this compound. However, at higher doses (2–5 nmoles, IT), d(CH2)5[Tyr(Me2)]AVP produced hindlimb paralysis, antinociception, and pressor responses by itself. In contrast to the fiber degeneration, cell loss, and necrosis found in lumbosacral cords of rats persistently paralyzed by other peptides (dynorphin A, somatostatin, and ICI 174864), neuropathological changes were not evident in spinal cords of rats transiently paralyzed by IT AVP. These results indicate that AVP-related peptides affected diverse spinal cord functions through interactions with a V1-like receptor. The similar pattern of cardiovascular and antinociceptive responses to other peptides (dynorphin A, somatostatin, and ICI 174864), which also caused hindlimb paralysis, suggests that the former responses may actually reflect the nonselective consequences of a peptide-induced disruption of spinal cord function, rather than specific shared pharmacological effects. 相似文献
35.
36.
在动物离心机上测定了7只轻度麻醉家免暴露于+G_Z时心、眼水平动脉压和心率的变化。+G_Z作用5-12s时、心水平动脉压(HABP)降至最低水平,然后开始代偿性回升。当+G_Z增大到一定值时,于加速度达峰值后,HABP降为0 mmHg、并在峰值后5.5±1.7s降到最大负值,继之代偿性升为正值,并常再度降为负值。我们称HABP的这种变化状态为“临界状态”。+G_Z暴露时,心率以两种型式发生改变:第一种,随着G值增大,心率发生不同程度增快,当加速度达某一G值时,心率突然减慢至2次/秒左右;第二种,当G值≥3时,在暴露过程中,心率逐渐减慢,并在某一G值,心率减慢到2次/秒左右。心率和HABP关系密切。当HABP达临界状态时,心率减慢至2次/秒左右并出现明显节律不整。以心率减慢到2次/秒左右作为家免+G_Z耐力终点是合适的,该指标规律性强,重复性好,实验方法对动物无损伤又易实施。按此标准,测得7只家免的+G_Z耐力为4.85±0.47G。 相似文献
37.
Mária Zsófia Koltai Peter Rösen Pál Hadházy Zoltán Aranyi György Ballagi-Pordány Gábor Pogátsa 《Molecular and cellular biochemistry》1992,109(2):189-196
The aim of this study was to clarify whether or not arachidonic acid metabolic disorders are caused by a substrate inavailability and whether such disorders might contribute to circulatory disturbances in the diabetic myocardium. Norepinephrine induced a decrease in the conductivity of both coronary arterial bed and myocardial microcirculation in alloxan-diabetic dogs. It was markedly (p < 0.05) attenuated both by indomethacin and acetylsalicylic acid pretreatments indicating an imbalance among the vasoactive prostanoids in diabetes. TXA2 release from the diabetic coronary rings was found to be elevated and could be normalized after the blockade of vascular adrenoceptors by phentolamine (p < 0.05). PGIZ synthesis was also enhanced by adrenergic blockade in the diabetic arterial rings. After pretreatment with l4C arachidonic acid, in order to measure substrate availability, the arachidonic acid metabolic rate was less in the diabetic coronary arteries than in healthy vessels (p < 0.05). Ten µmol/1 norepinephrine decreased arachidonic acid metabolism in the presence of prelabelled substrate in the diabetic animals, compared to an increase observed in metabolically healthy dogs. Therefore diabetes appears to diminish arachidonic acid metabolism and uptake independent of adrenoceptors and to induce an imbalance between vasoconstrictor and vasodilator cyclooxygenase products, resulting in elevated TXA2 release controlled by adrenergic mechanisms which may contribute to an impairment in myocardial microcirculation.Abbreviations 6-oxo-PGF1
6-oxo prostaglandin F1
- HPLC
High Pressure Liquid Chromatograph
- LAD
Left Anterior Descending (coronary artery)
- PGI2
Prostacyclin
- TXA2
Thromboxane 相似文献
38.
39.
The distribution of the pulmonary artery and vein of the orangutan lung was examined. The right pulmonary artery runs obliquely
across the ventral side of the right bronchus at the caudally to the right upper lobe bronchiole. It then runs across the
dorsal side of the right middle lobe bronchiole. Thereafter it runs obliquely across the dorsal side of the right bronchus,
and then along the dorso-medial side of the right bronchus. This course is different from that in other mammals. During its
course, it gives off branches which run mainly along the dorsal or lateral side of each bronchiole. The left pulmonary artery
runs across the dorsal side of the left middle lobe bronchiole, then along the dorso-lateral side of the left bronchus, giving
off branches which run along each bronchiole. The pulmonary veins run mainly the ventral or medial side of, along or between
the bronchioles. In the left lung, the left middle lobe vein has two trunks; one enters the left atrium, and the other enters
the left lower lobe pulmonary venous trunk. This is also different from that found in most mammals. Finally, the pulmonary
veins enter the left atrium with four large veins. 相似文献
40.
Summary The organization and distribution of microfilaments (MFs) in the preprophase bands (PPBs) of tobacco (Nicotiana tabacum L. var. Maryland Mammoth) root tip cells were studied with high pressure freezing and freeze-substitution methods. MFs were present predominantly as single filaments interspersed among microtubules (MTs) throughout the PPB. Some MFs appeared to be associated with MTs, whereas others were not. This is the first time that MFs have been demonstrated in the PPB at the electron microscope level. 相似文献