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Microfilaments in the preprophase band of freeze substituted tobacco root cells
Authors:B Ding  R Turgeon  M V Parthasarathy
Institution:(1) Section of Plant Biology, Division of Biological Sciences, Cornell University, 14853 Ithaca, NY, USA;(2) Present address: Department of Botany, University of California, Davis, California, USA
Abstract:Summary The organization and distribution of microfilaments (MFs) in the preprophase bands (PPBs) of tobacco (Nicotiana tabacum L. var. Maryland Mammoth) root tip cells were studied with high pressure freezing and freeze-substitution methods. MFs were present predominantly as single filaments interspersed among microtubules (MTs) throughout the PPB. Some MFs appeared to be associated with MTs, whereas others were not. This is the first time that MFs have been demonstrated in the PPB at the electron microscope level.
Keywords:Actin  Microfilaments  Microtubules  Preprophase band  Tobacco  High pressure freezing  Freeze substitution
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