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Peng-fei Duan 《农业工程》2019,39(1):77-80
Objectives
This paper aims to compare the property difference of spatial and temporal distribution of different nitrogen use efficiency maize genotypes and discuss the physiological mechanism of nitrogen efficiency of maize.Method
In this study, phenotype of root crowns of maize in seedling stage (V5), bell stage (V12) and silking stage (R1) was conducted to discover phenes and phene modules related to N acquisition. An image was captured for the whole roots. Custom software was used to measure root phenes including root area, root projected structure length, maximum width of roots, and root angle. The study was conducted to examine the differences in spatial and temporal distribution of maize root at two nitrogen levels (0 and 240?kg hm-2), high efficiency genotype ZHENGDAN958 (ZD958) and low efficiency genotype DANYU13 (DY13) are used in field production. Under the low nitrogen stress conditions, the root area of ZD958 significantly increased after bell stage and exceeds the CK by 20.2% at silking stage. With LN, the root projected structure length of ZD958 was longer than that of CK by 49.4% at silking stage. The low efficiency genotype DY13 had no obvious change at two nitrogen levels. The number of the grain yield and root system biomass of high efficiency genotype ZD958 was remarkably larger than that of low efficiency genotype DY13.Conclusion
During the whole growth stage, the root dry weight, root area and the root width med of high efficiency genotype ZD958 were larger than that of high efficiency genotype DY13. Besides, under the low nitrogen stress conditions, the width of ZD958 deep soil root tended to become longer which is good for the nitrogen absorption from the deep soil. High efficiency genotype can construct a root system which is in a well-developed and reasonable spatial distribution before blooming, so as to ensure plant nitrogen absorption and biomass generation as well. The root index of the nitrogen responsivity of high efficiency genotype ZD958 was higher than that of low efficiency genotype DY13 before blooming. 相似文献42.
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NRSE 与 NRSF 及其对神经元特异性基因表达的调控作用 总被引：3，自引：0，他引：3
神经限制性沉默元件 (NRSE) 是一段长度为 21~23 bp 的保守 DNA 序列,存在于许多神经元特异表达基因的转录调控区中,神经限制性沉默因子 (NRSF) 能特异性结合到 NRSE dsDNA 上,并通过其 N 端和 C 端阻遏结构域分别连接共阻遏蛋白 Sin3A/B 和 CoREST , Sin3A 招募 HDAC 对组蛋白进行去乙酰基化修饰, CoREST 则作为平台蛋白招募特异的“沉默组件”,以此维持基因沉默 . 最近的研究显示, NRSE dsRNA 能在转录水平与 NRSF 蛋白直接作用,而不是作为 siRNA 或 miRNA 在转录后水平启动神经元特异性基因的表达 . 相似文献
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S Singh M Ramamoorthy C Vaughan W A Yeudall S Deb S Palit Deb 《Cell death and differentiation》2013,20(4):558-566
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Kaichi Yoshizaki Ryuichi Kimura Hisato Kobayashi Shinya Oki Takako Kikkawa Lingling Mai Kohei Koike Kentaro Mochizuki Hitoshi Inada Yasuhisa Matsui Tomohiro Kono Noriko Osumi 《EMBO reports》2021,22(2)
Advanced paternal age can have deleterious effects on various traits in the next generation. Here, we establish a paternal‐aging model in mice to understand the molecular mechanisms of transgenerational epigenetics. Whole‐genome target DNA methylome analyses of sperm from aged mice reveal more hypo‐methylated genomic regions enriched in REST/NRSF binding motifs. Gene set enrichment analyses also reveal the upregulation of REST/NRSF target genes in the forebrain of embryos from aged fathers. Offspring derived from young mice administrated with a DNA de‐methylation drug phenocopy the abnormal vocal communication of pups derived from aged fathers. In conclusion, hypo‐methylation of sperm DNA can be a key molecular feature modulating neurodevelopmental programs in offspring by causing fluctuations in the expression of REST/NRSF target genes. 相似文献
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