全文获取类型
收费全文 | 80588篇 |
免费 | 3341篇 |
国内免费 | 7200篇 |
出版年
2023年 | 706篇 |
2022年 | 917篇 |
2021年 | 1452篇 |
2020年 | 1548篇 |
2019年 | 2865篇 |
2018年 | 1868篇 |
2017年 | 1548篇 |
2016年 | 1952篇 |
2015年 | 3092篇 |
2014年 | 4081篇 |
2013年 | 5688篇 |
2012年 | 3467篇 |
2011年 | 4852篇 |
2010年 | 3532篇 |
2009年 | 3605篇 |
2008年 | 3825篇 |
2007年 | 4077篇 |
2006年 | 3704篇 |
2005年 | 3233篇 |
2004年 | 2701篇 |
2003年 | 2392篇 |
2002年 | 2104篇 |
2001年 | 1689篇 |
2000年 | 1487篇 |
1999年 | 1483篇 |
1998年 | 1386篇 |
1997年 | 1199篇 |
1996年 | 1067篇 |
1995年 | 1310篇 |
1994年 | 1193篇 |
1993年 | 1159篇 |
1992年 | 1176篇 |
1991年 | 987篇 |
1990年 | 907篇 |
1989年 | 863篇 |
1988年 | 860篇 |
1987年 | 831篇 |
1986年 | 552篇 |
1985年 | 980篇 |
1984年 | 1371篇 |
1983年 | 974篇 |
1982年 | 1331篇 |
1981年 | 937篇 |
1980年 | 944篇 |
1979年 | 898篇 |
1978年 | 522篇 |
1977年 | 425篇 |
1976年 | 342篇 |
1975年 | 267篇 |
1973年 | 270篇 |
排序方式: 共有10000条查询结果,搜索用时 263 毫秒
991.
Abstract Acetobacterium woodii was continuously grown on 3,4,5-trimethoxybenzoate as pure culture or in commensalistic combination with Pelobacter acidigallici and Desulfobacter postgatei . Under pure culture conditions the following growth parameters were determined: μ max = 0.112 h−1 , K s = 1.07 mM, Y max = 35 g/mol, and m = 0.22 mmol·g−1 ·h−1 . In coculture with P. acidigallici the affinity for the substrate increased and the K s value was found to be 135 μM. Under batch culture conditions mixed populations of A. woodii, P. acidigallici , and D. postgatei completely mineralized 3,4,5-trimethoxybenzoate to CO2 , whereas under continuous culture conditions more than 3 mM acetate remained unused. 相似文献
992.
Megumi Kono Koji O'hara Yoshihiro Arai Hideyuki Fukuda Misaki Asakawa Hideomi Nakahara 《FEMS microbiology letters》1985,28(2):213-217
Abstract A susceptibility of 63 clinical isolates of Klebsiella pneumoniae to inorganic and organic mercuric compounds was determined. 18 of them were found to be resistant to fluorescein mercuric acetate (FMA) and merbromin (MB). Moreover, all the resistant strains inactivate the antibacterial effect of FMA. The changes in the amount of organic mercury at the time of inactivation of the drug and the structures of the end products were examined in detail with the plasmid-bearing strain JK9 and its transconjugants of Escherichia coli .
The results showed that FMA was inactivated by an intracellular enzyme produced inducively and was degraded to fluorescein (sodium salt, uranine), which led to the sedimentation of metallic mercury. The discovery of the genes conferring inducible organic mercury-inactivating enzymes determined by plasmids was the next step and their application in the recovery of metallic mercury from organomercurials is now imminent. 相似文献
The results showed that FMA was inactivated by an intracellular enzyme produced inducively and was degraded to fluorescein (sodium salt, uranine), which led to the sedimentation of metallic mercury. The discovery of the genes conferring inducible organic mercury-inactivating enzymes determined by plasmids was the next step and their application in the recovery of metallic mercury from organomercurials is now imminent. 相似文献
993.
994.
995.
996.
997.
998.
Abstract Spore-forming sulfate-reducing bacteria (SRB) were enriched selectively from various kinds of aerobic soils with fatty acids as the sole carbon and energy source. A Gram-negative motile rod-shaped bacterium, which produced gas vacuoles during sporulation was isolated. It degraded alcohols, aromatic and n-fatty acids (up to C18 ) except for propionate, completely to CO2 . Sulfate, sulfite, thiosulfate or elemental sulfur served as electron acceptors. Because of its sensitivity to H2 S, the isolate never produced more than 8 mM dissolved sulfide at pH 7.0. G + C-content of the DNA was 48.0 mol %. The isolated strain Pato is described as a new species Desulfotomaculum sapomandens . 相似文献
999.
Molecular cloning and nucleotide sequences of cDNAs specific for rat liver ribosomal proteins S17 and L30 总被引:10,自引:0,他引:10
O Nakanishi M Oyanagi Y Kuwano T Tanaka T Nakayama H Mitsui Y Nabeshima K Ogata 《Gene》1985,35(3):289-296
cDNA clones coding for rat liver ribosomal proteins S17 and L30 have been isolated by positive hybridization-translation assay from a cDNA library prepared from 8-9S poly(A)+RNA from free polysomes of regenerating rat liver. The cDNA clone specific for S17 protein (pRS17-2) has a 466-bp insert with the poly(A) tail. The complete amino acid (aa) sequence of S17 protein was deduced from the nucleotide sequence of the cDNA. S17 protein consists of 134 aa residues with an Mr of 15 377. The N-terminal aa sequence of S17 protein determined by automatic Edman degradation is consistent with the sequence data. The aa sequence of S17 shows strong homology (76.9%) to that of yeast ribosomal protein 51 [Teem and Rosbash, Proc. Natl. Acad. Sci. USA 80 (1983) 4403-4407] in the two-thirds N-terminal region. The cDNA clone specific for L30 protein (pRL30) has a 394-bp insert. The aa sequence of L30 protein was deduced from the nucleotide sequence of the cDNA. The protein consists of 114 aa residues with an Mr of 12 652. When compared with the N-terminal aa sequence of rat liver L30 protein [Wool, Annu. Rev. Biochem. 48 (1979) 719-754], pRL30 was found not to contain the initiation codon and 5'-noncoding region. The cDNA showed twelve silent changes in the coding region, one point mutation and one base deletion in the 3'-noncoding region, compared with mouse genomic DNA for L30 protein [Wiedemann and Perry, Mol. Cell Biol. 4 (1984) 2518-2528]. 相似文献
1000.
Abstract Proton translocation associated with electron flow to oxygen has been observed with cells of Nitrobacter winogradskyi in the presence of either potassium ferrocyanide or isoascorbate plus N , N , N ', N ' tetramethyl- p -phenylenediamine. The data are consistent with a proton pumping function for the terminal oxidase, cytochrome aa 3 , in this organism as the mechanism for generating a protonmotive force. The failure of previous work with Nitrobacter [4] to detect proton translocation linked to oxidation of nitrite, the physiological substrate, is discussed. 相似文献