Octopine Ti-plasmid deletion mutants of agrobacterium tumefaciens with emphasis on the right side of the T-region

One hundred and twelve Agrobacterium tumefaciens mutants with a deleted octopine Ti plasmid were isolated. They originated from four insertion mutants, each of which carried the transposon Tn904 at a different position in the Ti plasmid. The deletion mutants were selected on the basis of loss of the capacity encoded by the Ti plasmid to degrade octopine. They were tested for the expression of other Ti-plasmid coded functions: tumor induction, presence of lysopine dehydrogenase activity in the tumor, and exclusion of phage Ap-1. For 21 mutants affected in at least one of these functions, the map position of the deletions was determined. It was found that deletions at two separated loci give rise to an Occ^? phenotype. Genes for Ap-1 exclusion were mapped on a small region just outside and to the right of the T_L + T_R region. Most of the T_R region, present as T_R-DNA in a limited number of crown gall tissues only, was shown to be unnecessary for tumor formation, since it could be deleted without affecting virulence (tested on various plant species) of the mutants. However, if the T_R region together with a small part of the adjacent T_L region, which is always present as T_L-DNA in normal crown gall tissues, was deleted the mutants became weakly virulent on Kalanchoë and Nicotiana rustica and avirulent on tomato. We hypothesize that in this case a region necessary for T-DNA integration has been deleted. The same region was found to be essential for lysopine dehydrogenase activity in the tumors.     

Digitization of sedimentation equilibrium and velocity data for analysis by minicomputer

A procedure is described whereby phosphorylated seryl residues may be unequivocally identified during the sequential degradation of a polypeptide chain by the Edman technique. The phosphoseryl residue, Ser(P), was first converted by treatment with methylamine in dilute alkali to a β-methylaminoalanyl residue which was split from the polypeptide by the degradative procedure as the derived phenylthiohydantoin. This was identified by high-performance liquid chromatography. The procedure was highly effective when the Ser(P) occupied an isolated position in a polypeptide chain but was less so when grouped consecutively with other Ser(P).     

Mouse pre-B cells synthesize and secrete mu heavy chains but not light chains

The immunoglobulins produced by the earliest recognizable B cell precursors (pre-B cells) were characterized in the mouse and human. Immunofluorescent analysis revealed no evidence of surface IgM components, and only mu heavy chains could be detected intracytoplasmically in pre-B cells. Surface IgM components could not be isolated from intact fetal liver cells that lacked sIgM+ B lymphocytes but possessed pre-B cells. Pre-B cells were shown to synthesize and secrete mu heavy chains but not light chains by immunochemical analysis. These mu chains constituted less than 0.01% of TCA precipitable protein synthesized and secreted by fetal liver cells during an 8 hr labelling period. Migration of both intracellular and secreted mu chains on SDS-PAGE suggested that they were smaller than mu chains secreted by mouse and human plasmacytomas. These data indicate that mu chain synthesis precedes light chain expression during B cell ontogeny and suggest a new role for pre-B cells in the generation and expression of a diverse immunoglobulin repertoire.     

高山植物圆穗蓼的繁殖资源分配

于海北高寒草甸生态系统定位站附近的一个西向山坡上,以高寒灌丛草甸中海拔不同的4个地点的圆穗蓼为研究对象,分析圆穗蓼的繁殖特征在海拔梯度上的变化规律,以探讨高山植物在极端环境下的资源分配策略。结果发现:(1)随海拔升高,圆穗蓼的植株高度先增大后变小,营养器官生物量和繁殖器官生物量均呈现增加的趋势,但三者与海拔梯度的相关性不显著,而且繁殖分配在4个不同海拔取样地点间差异不显著;(2)个体越大,圆穗蓼分配到繁殖器官的绝对资源比例也显著增加,但在海拔较低的3个取样地点内,繁殖分配随海拔升高呈增加的趋势,这可能与高寒灌丛草甸土壤的潜在肥力较高而导致圆穗蓼不存在资源限制有关;(3)圆穗蓼在4个取样地点均存在繁殖阈值,繁殖阈值与海拔的相关性不显著,但仍然表现出随海拔升高而略有降低的趋势,表明海拔较高居群的植株达到开花繁殖需要的营养积累较小。研究表明,由于海北站的土壤肥力较高,4个取样地点的圆穗蓼不存在个体大小依赖性的繁殖分配和随海拔升高而增加的繁殖分配;而高海拔取样地点较低的繁殖阈值表明高山植物的有性繁殖比营养生长具有更为重要的意义,强调了有性繁殖对高山植物的重要性。     

Influences of travertine dam formation on leaf litter decomposition and algal accrual

At the time of this study Fossil Creek was being considered as a site for the restoration of a native fish assemblage, however there was concern amongst fisheries managers about the stream being food limited due to calcium carbonate (travertine) deposition. To evaluate the effects of travertine deposition on the aquatic food base we used leaf litterbags to compare decomposition rates and nutrient diffusing artificial substrates to compare algal accrual rates and nutrient limitation between two distinct reaches in Fossil creek: a travertine dam forming reach and a reach without travertine dam formation (riffle-pool reach). Decomposition was significantly faster in the travertine dam forming reach than in the riffle-pool reach. Macroinvertebrates in the leaf packs were more diverse in the travertine reach but more abundant in the riffle-pool reach. Algae accrued more quickly in the travertine reach than in the riffle-pool reach and only responded to nutrient enrichment in the travertine reach. This study was conducted prior to a hydroelectric dam decommissioning project in Fossil Creek where full flows were reintroduced back into the stream after a century of diversion. Our results suggest concurrent increases in algal productivity, decomposition, and macroinvertebrate diversity in the next decade as travertine dams rebuild, providing a richer food base for fish and other aquatic organisms. Electronic supplementary material Electronic supplementary material is available for this article at and accessible for authorised users. Handling editor: K. Martens     

High population density of black-handed spider monkeys (<Emphasis Type="Italic">Ateles geoffroyi</Emphasis>) in Costa Rican lowland wet forest

The main objective of this study was to estimate the population density and demographic structure of spider monkeys living in wet forest in the vicinity of Sirena Biological Station, Corcovado National Park, Costa Rica. Results of a 14-month line-transect survey showed that spider monkeys of Sirena have one of the highest population densities ever recorded for this genus. Density estimates varied, however, depending on the method chosen to estimate transect width. Data from behavioral monitoring were available to compare density estimates derived from the survey, providing a check of the survey’s accuracy. A combination of factors has most probably contributed to the high density of Ateles, including habitat protection within a national park and high diversity of trees of the fig family, Moraceae. Although natural densities of spider monkeys at Sirena are substantially higher than those recorded at most other sites and in previous studies at this site, mean subgroup size and age ratios were similar to those determined in previous studies. Sex ratios were similar to those of other sites with high productivity. Although high densities of preferred fruit trees in the wet, productive forests of Sirena may support a dense population of spider monkeys, other demographic traits recorded at Sirena fall well within the range of values recorded elsewhere for the species.     

WEDDELL SEAL UNDERWATER CALLING RATES DURING THE WINTER AND SPRING NEAR MAWSON STATION, ANTARCTICA

Underwater vocalization monitoring and surveys, both on ice and underwater, were used to determine if Weddell seals ( Leptonychotes weddellii ) near Mawson Station, Antarctica, remain under the fast ice during winter within close range of breeding sites. Daytime and nighttime underwater calling rates were examined at seven breeding sites during austral winter and spring to identify seasonal and diel patterns. Seals rarely hauled out at any of the sites during winter, although all cohorts (adult males, females, and juveniles) were observed underwater and surfacing at breathing holes throughout winter (June–September) and spring (October–December). Seal vocalizations were recorded during each sampling session throughout the study ( n = 102 daytime at seven sites collectively, and n = 5 24-h samples at each of two sites). Mean daytime calling rate was low in mid-winter (July) (mean = 18.9 ± 7.1 calls/min) but increased monthly, reaching a peak during the breeding season (November) (mean = 62.6 ± 15.7 calls/min). Mean nighttime calling rate was high throughout the winter and early spring (July–October) with mean nocturnal calling rate in July (mean = 61.8 ± 35.1 calls/min) nearly equal to mean daytime calling rate in November (during 24-h daylight). Reduced vocal behavior during winter daylight periods may result from animals utilizing the limited daylight hours for nonvocal activities, possibly feeding.     

Kamchatka Marine Research Station: The 75th anniversary of foundation

The history of the founding the Kamchatka Marine Research Station of the State Hydrological Institute and the major results of scientific investigations conducted in 1931–1935 are outlined in this paper.     

Patterns in a species‐rich tropical understory plant community

Understory plants are an important component of the high plant species diversity characteristic of neotropical rain forests. Herbs, shrubs, understory trees, and saplings of canopy trees occupy a broadly uniform environment of abundant rainfall, low light levels, and high humidity. We asked whether this community at the La Selva Biological Station in the Caribbean lowlands of Costa Rica was structured by environmental filters such as soil origin, topographic position, and understory light availability. We used nested quadrats to assess effects of soil origin (recent alluvium, weathered alluvium, residual volcanic soil) and topographic position (ridges, mid‐slopes and flats) on species composition, density, and diversity and measured six edaphic and understory light parameters. Non‐metric multidimensional scaling ordinations were based on frequency of occurrence in 20 quadrats for 272 species in the shrub size class and 136 species in the small‐tree size class for 17 sites. Three axes were correlated with composite environmental variables produced by principal component analysis representing slope, extractable phosphorus, and light. NMS site positions also reflected soil origin, topographic position, and geographic location. The analyses illustrated a complex community structured by species responses to environmental filters at multiple, interdigitated spatial scales. We suggest that light availability affected by canopy dynamics and dispersal limitation provides additional sources of variation in species distributions, which interact with edaphic patterns in complex ways. Abstract in Spanish is available with online material.     

Fruit production is influenced by tree size and size‐asymmetric crowding in a wet tropical forest

In tropical forest communities, seedling recruitment can be limited by the number of fruit produced by adults. Fruit production tends to be highly unequal among trees of the same species, which may be due to environmental factors. We observed fruit production for ~2,000 trees of 17 species across 3 years in a wet tropical forest in Costa Rica. Fruit production was modeled as a function of tree size, nutrient availability, and neighborhood crowding. Following model selection, tree size and neighborhood crowding predicted both the probability of reproduction and the number of fruit produced. Nutrient availability only predicted only the probability of reproduction. In all species, larger trees were more likely to be reproductive and produce more fruit. In addition, number of fruit was strongly negatively related to presence of larger neighboring trees in 13 species; presence of all neighboring trees had a weak‐to‐moderate negative influence on reproductive status in 16 species. Among various metrics of soil nutrient availability, only sum of base cations was positively associated with reproductive status, and for only four species. Synthesis Overall, these results suggest that direct influences on fruit production tend to be mediated through tree size and crowding from neighboring trees, rather than soil nutrients. However, we found variation in the effects of neighbors and nutrients among species; mechanistic studies of allocation to fruit production are needed to explain these differences.