全文获取类型
收费全文 | 2039篇 |
免费 | 108篇 |
国内免费 | 572篇 |
出版年
2024年 | 2篇 |
2023年 | 26篇 |
2022年 | 32篇 |
2021年 | 51篇 |
2020年 | 61篇 |
2019年 | 73篇 |
2018年 | 58篇 |
2017年 | 73篇 |
2016年 | 98篇 |
2015年 | 79篇 |
2014年 | 71篇 |
2013年 | 110篇 |
2012年 | 86篇 |
2011年 | 89篇 |
2010年 | 65篇 |
2009年 | 128篇 |
2008年 | 137篇 |
2007年 | 171篇 |
2006年 | 114篇 |
2005年 | 102篇 |
2004年 | 85篇 |
2003年 | 95篇 |
2002年 | 84篇 |
2001年 | 80篇 |
2000年 | 67篇 |
1999年 | 40篇 |
1998年 | 48篇 |
1997年 | 32篇 |
1996年 | 84篇 |
1995年 | 38篇 |
1994年 | 34篇 |
1993年 | 30篇 |
1992年 | 38篇 |
1991年 | 38篇 |
1990年 | 42篇 |
1989年 | 27篇 |
1988年 | 25篇 |
1987年 | 21篇 |
1986年 | 39篇 |
1985年 | 14篇 |
1984年 | 16篇 |
1983年 | 5篇 |
1982年 | 22篇 |
1981年 | 12篇 |
1980年 | 10篇 |
1979年 | 18篇 |
1978年 | 15篇 |
1977年 | 18篇 |
1976年 | 13篇 |
1975年 | 2篇 |
排序方式: 共有2719条查询结果,搜索用时 15 毫秒
171.
Integrated aquaculture in Chinese lakes and paddy fields 总被引:1,自引:0,他引:1
While pond culturing is still the staple of Chinese freshwater fisheries, its extensive development is handicapped by the increasing shortage of land resource for cereal production in face of China’s population growth. Accordingly, people have looked towards the development of aquaculture in lakes (and reservoirs) in the 1970s. This paper reviews the successful integrated measures taken in an experiment for raising fish in Donghu lake (East lake) of Wuhan. By way of (1) stocking the right kinds of fish; (2) producing a large number of sizeable fingerlings; (3) improving fish screens; (4) controlling predatory fish; and (5) applying the ‘driving and concentrating’ bulk harvesting fishing method, fish production of the lake has increased steadily from 180 t in 1971 to 800 t in 1978, and then to 1840 t in 1995, all in an area of 1500 ha, and all without any supplementary feeding. Integrated aquaculture in paddy fields, known to be beneficial to both rice and fish production, has been regaining momentum in recent years. The paper summarizes its underlying principle, its benefits—economical, social and ecological—and the prospect of its development in China. 相似文献
172.
Assortative mating among rock-dwelling cichlid fishes supports high estimates of species richness from Lake Malawi 总被引:8,自引:1,他引:7
MADELEINE J. H. VAN OPPEN GEORGE F. TURNER CIRO RICO ROSANNA L. ROBINSON JAMES C. DEUTSCH MARTIN J. GENNER & GODFREY M. HEWITT 《Molecular ecology》1998,7(8):991-1001
It has been estimated that Lake Malawi, Africa, contains 500–650 endemic species of cichlid fishes, the largest number of vertebrate species endemic to any comparable sized area on the planet. As many of these putative species cannot be distinguished anatomically, these estimates of species richness depend to a great extent on the assumption that sympatrically occurring male colour morphs represent biological species. We have tested this assumption using a combination of behavioural observations of courtship and microsatellite DNA analysis for six putative species of the Pseudotropheus ( Tropheops ) complex and three of the Pseudotropheus ( Maylandia ) complex occurring sympatrically at Nkhata Bay. We were unable to demonstrate assortative courtship for the species pairs Pseudotropheus ( Maylandia ) zebra / P . 'gold zebra' or P. ( Tropheops ) 'band'/ P. ( T .) 'rust' because we were unable to distinguish between the females of these taxa. All other taxa showed clear assortative courtship, except for P. ( T .) 'deep', a deep-water species which was rarely observed. Fixation indices (θST for the infinite allele model, and R ST for the stepwise mutation model) calculated from six microsatellite DNA loci demonstrated significant deviations from panmixia in all pairwise comparisons of putative species, indicating little or no gene flow between populations. All taxa showed high levels of allelic diversity providing evidence that genetic bottlenecking may have been of limited importance in the speciation process. Assortative mating among taxa differing only in male colouration is supportive of theories that speciation in these fishes has been driven by sexual selection by female choice. 相似文献
173.
应用人工神经网络评价湖泊的富营养化 总被引:18,自引:1,他引:17
应用人工神经网络方法,以化学需氧量、总氮、总磷和透明度作为评价参数,经反复尝试,构建了具有4层结构用于评价湖泊富营养化的误差逆传播网络.其输入层有4个神经元,2个隐含层也各有4个神经元,输出层有1个神经元.以太湖富营养化评价标准作为样本模式提供给网络,按照误差逆传播网络的学习规则对网络进行训练,经过37684次学习后,网络达到预先给定的收敛标准.使网络具备了识别湖泊富营养化程度的功能.应用该网络对我国17个湖泊的富营养化程度进行评价,操作过程简便易行,评价结果切合实际,展示了这种方法的一系列优点. 相似文献
174.
Intraspecific brood mixing and reduced polyandry in a maternal mouth-brooding cichlid 总被引:3,自引:1,他引:2
Kellogg Karen A.; Markert Jeffrey A.; Stauffer Jay R. Jr.; Kocher Thomas D. 《Behavioral ecology》1998,9(3):309-312
Microsatellite loci were used to evaluate the level of polyandryand intraspecific brood mixing in Protomelas c.f. spilopterus,a paedophagous, maternal mouth-brooding cichlid from Lake MakaiAfrica. We found that broods were fertilized by one to threemales, which was a reduced level of multiple paternity comparedto other mouth-brooding cichlids. Low density of breeding malesand the risk of intraspecific predation are likely explanationsfor reduced polyandry. Intraspecific brood-mixing was foundin four out of the six broods examined, with the proportionsof foreign fry ranging from 6% to 65%. The potential originsof brood mixing are discussed, although no firm conclusionscan be drawn given the limited behavioral observations for thisspecies. 相似文献
175.
176.
《Harmful algae》2015
The filamentous cyanobacterium Planktothrix rubescens produces secondary metabolites called microcystins (MC) that are potent toxins for most eukaryotes, including zooplankton grazers, cattle and humans. P. rubescens occurs in many deep and thermally stratified lakes throughout Europe. In Lake Zurich (Switzerland), it re-appeared in the 1970s concomitant with decreasing eutrophication. Since then, P. rubescens has become the dominant species in this major drinking water reservoir, where it forms massive metalimnetic blooms during late summer. These cyanobacteria harbor subpopulations of non-MC producers, but little is known about the environmental factors affecting the success of such genotypes. The non-MC-producing subpopulation of P. rubescens was studied using a quantitative real-time PCR (qPCR) assay on the MC synthetase (mcy) gene cluster that targets a deletion on the mcyH and mcyA genes, which inactivates MC biosynthesis. Two complementary qPCR assays were used to assess the total population abundance (based on the 16S rDNA gene) and the mcy gene copy number (based on a conserved region in the adenylation domain of the mcyB gene). The objective was to evaluate the seasonal patterns of the share of non-MC-producing filaments in the total P. rubescens population. The mcyHA mutants were present in low proportions (up to 14%) throughout the year. Their highest relative abundances occurred during the winter mixis, when total concentrations of P. rubescens were minimal. The MC deficient mutants seemed to better survive in sparse populations, possibly because of lower grazing pressure and a consequently reduced need for MC-mediated protection. Alternatively, the mutants might cope better with the sub-optimal, stressful pressure and light conditions during the winter mixis. Altogether, our results suggest that subtle trade-offs might seasonally determine the proportions of non-MC producers within P. rubescens populations. 相似文献
177.
178.
179.
Sexual selection by female mating preference for male nuptial coloration has been suggested as a driving force in the rapid speciation of Lake Victoria cichlid fish. This process could have been facilitated or accelerated by genetic associations between female preference loci and male coloration loci. Preferences, as well as coloration, are heritable traits and are probably determined by more than one gene. However, little is known about potential genetic associations between these traits. In turbid water, we found a population that is variable in male nuptial coloration from blue to yellow to red. Males at the extreme ends of the phenotype distribution resemble a reproductively isolated species pair in clear water that has diverged into one species with blue-grey males and one species with bright red males. Females of the turbid water population vary in mating preference coinciding with the male phenotype distribution. For the current study, these females were mated to blue males. We measured the coloration of the sires and male offspring. Parents-offspring regression showed that the sires did not affect male offspring coloration, which confirms earlier findings that the blue species breeds true. In contrast, male offspring coloration was determined by the identity of the dams, which suggests that there is heritable variation in male color genes between females. However, we found that mating preferences of the dams were not correlated with male offspring coloration. Thus, there is no evidence for strong genetic linkage between mating preference and the preferred trait in this population [Current Zoology 56 (1): 57-64 2010]. 相似文献
180.
微囊藻毒素在滇池鱼体内的积累水平及分布特征 总被引:2,自引:0,他引:2
为了解富营养化水体中鱼体内微囊藻毒素(MC)的积累水平及其分布特征,2003年4月和9月份两次在滇池试验区采集了鲢、鳙和草鱼等鱼种,用ELISA方法对鱼体中肝、肾、空肠、胆、肌肉等不同组织中MC的含量进行了检测。结果表明,MC在所有样品中均能检测到,且主要分布在鱼体的肝肾脏和消化道等器官,而肌肉和非消化道器官中毒素含量相对较低。不同鱼种不同组织对MC的富集程度也明显不同,鲢鳙中肝脏和肾脏这两个主要的靶器官对MC的蓄积能力就远高于草鱼。同时,不同季节MC在鱼体内的积累水平也明显不同,4月份鱼样中MC的含量普遍低于9月份鱼样中MC的含量。最后按照WHO生活饮用水安全标准的建议进行推算,所有鱼肉中的MC均没有超过其推荐的人体每日可允许摄入量(≤0.04μg/kg人体重),初步推断鱼肉中MC暂时还未危及到人体健康,但仍具有潜在的风险性。 相似文献