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41.
Growth conditions play a major role in expression of virulence by Shigella spp. both in vitro (adherence and internalization in eukaryotic host cells) and in vivo (keratoconjunctivitis). Optimized expression of virulence required anaerobic growth to log phase in particular media such as brain heart infusion broth. Kinetic studies of guinea pig eye infections showed that as few as 2 x 10(5) S. dysenteriae CG097 or S. flexneri M90T, grown under these optimized conditions, produced keratoconjunctivitis in 15 h. In vitro studies demonstrated that adherence to and invasion of Henle 407 cells, at 37 degrees C, by organisms grown under these optimized conditions, were significantly greater than when organisms were grown aerobically under the same conditions. 相似文献
42.
Trichostatin A,an Inhibitor of Histone Deacetylase,Inhibits the Viability and Invasiveness of Hypoxic Rheumatoid Arthritis Fibroblast‐Like Synoviocytes via PI3K/Akt Signaling
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This study was undertaken to explore the effects of trichostatin A (TSA), an inhibitor of histone deacetylase, on the viability, apoptosis, and invasiveness of hypoxic rheumatoid arthritis fibroblast‐like synoviocytes (RA FLSs). RA FLSs were exposed to hypoxia for 24 h in the presence or absence of 2 μM TSA and tested for cell viability, apoptosis, invasion, and gene expression. The involvement of the phosphatidylinositol‐3‐kinase (PI3K)/Akt pathway was checked. TSA significantly inhibited the viability and induced apoptosis of hypoxic RA FLSs, compared to vehicle control. TSA blocked hypoxia‐induced invasion of RA FLSs during Matrigel invasion assays and reduced the expression of matrix metalloproteinases (MMP‐2 and MMP‐9) and PI3K and phosphorylation of Akt. Overexpression of constitutively active Akt reversed TSA‐mediated suppression of invasiveness and downregulation of MMP‐2 and MMP‐9. Our results indicate the antisurvival and antiinvasive activities of TSA in hypoxic RA FLSs, which is associated with inactivation of PI3K/Akt signaling. 相似文献
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Nitrogen allocation,partitioning and use efficiency in three invasive plant species in comparison with their native congeners 总被引:2,自引:0,他引:2
In this study, we hypothesized that invasive species may allocate a higher fraction of leaf nitrogen (N) to photosynthesis
than phylogenetically related native species. To test this hypothesis, we determined N allocation and other ecophysiological
traits of three invasive species in comparison with their respective native congeners by measuring response curves of photosynthesis
to intercellular CO2 concentration. The invasive species of Peperomia and Piper indeed allocated a higher fraction of leaf N to photosynthesis and were more efficient in photosynthetic N (N
P) partitioning than their native congeners. The two invasive species partitioned a higher fraction of N
P to carboxylation and showed a higher use efficiency of N
P, while their native congeners partitioned a higher fraction of N
P to light-harvesting components. The higher N allocation to photosynthesis and the higher N
P partitioning to carboxylation in the two invaders were associated with their higher specific leaf area. Nitrogen allocation
and partitioning were the most important factors in explaining the differences in light-saturated photosynthetic rate and
photosynthetic N use efficiency (PNUE) between the two invasive species and their native congeners. The differences in N allocation-related
variables between the invasive and native species of Amaranthus could not be evaluated in this study due to the method. Except PNUE, resource capture- and use-related traits were not always
higher in all three invasive species compared to their native congeners, indicating that different invasive species may have
different syndrome of traits associated with its invasiveness. 相似文献
46.
Plant species diversity as a driver of early succession in abandoned fields: a multi-site approach 总被引:15,自引:0,他引:15
W. H. Van der Putten S. R. Mortimer K. Hedlund C. Van Dijk V. K. Brown J. Lepä C. Rodriguez-Barrueco J. Roy T. A. Diaz Len D. Gormsen G. W. Korthals S. Lavorel I. Santa Regina P. Smilauer 《Oecologia》2000,124(1):91-99
Succession is one of the most studied processes in ecology and succession theory provides strong predictability. However,
few attempts have been made to influence the course of succession thereby testing the hypothesis that passing through one
stage is essential before entering the next one. At each stage of succession ecosystem processes may be affected by the diversity
of species present, but there is little empirical evidence showing that plant species diversity may affect succession. On
ex-arable land, a major constraint of vegetation succession is the dominance of perennial early-successional (arable weed)
species. Our aim was to change the initial vegetation succession by the direct sowing of later-successional plant species.
The hypothesis was tested that a diverse plant species mixture would be more successful in weed suppression than species-poor
mixtures. In order to provide a robust test including a wide range of environmental conditions and plant species, experiments
were carried out at five sites across Europe. At each site, an identical experiment was set up, albeit that the plant species
composition of the sown mixtures differed from site to site. Results of the 2-year study showed that diverse plant species
mixtures were more effective at reducing the number of natural colonisers (mainly weeds from the seed bank) than the average
low-diversity treatment. However, the effect of the low-diversity treatment depended on the composition of the species mixture.
Thus, the effect of enhanced species diversity strongly depended on the species composition of the low-diversity treatments
used for comparison. The effects of high-diversity plant species mixtures on weed suppression differed between sites. Low-productivity
sites gave the weakest response to the diversity treatments. These differences among sites did not change the general pattern.
The present results have implications for understanding biological invasions. It has been hypothesised that alien species
are more likely to invade species-poor communities than communities with high diversity. However, our results show that the
identity of the local species matters. This may explain, at least partly, controversial results of studies on the relation
between local diversity and the probability of being invaded by aliens.
Received: 13 July 1999 / Accepted: 4 February 2000 相似文献
47.
Eupatorium adenophorum is one of the more noxious invasive plants worldwide. However, the mechanisms underlying its invasiveness are still not well
elucidated. In this study, we compared the invader with its two native congeners (E. heterophyllum and E. japonicum) at four irradiances in terms of growth, biomass allocation, morphology, and photosynthesis. The higher light-saturated photosynthetic
rate (P
max) and total leaf area of the invader may contribute to its higher relative growth rate (RGR) and total biomass compared with
its native congeners. Total biomass and RGR increased significantly with the increase of P
max and total leaf area. The higher support organ mass fraction and the lower root mass fraction of the invader may also contribute
to its higher RGR and biomass through increasing carbon assimilation and reducing respiratory carbon loss, respectively. The
higher growth rate of the invader increased its total leaf area, ramet number, and crown area. These traits may help the invader
to form dense monoculture, outshading native plant species. However, consistently higher leaf area ratio, specific leaf area,
and leaf mass fraction were not found across irradiances for the invader compared with its native congeners. Higher plasticity
in response to irradiance was also not found for the invader. The invader retained advantages over the natives across irradiances,
while its performance decreased with lower irradiance. The results indicate that the invader may be one of the few super invaders.
Reducing irradiance may inhibit its invasions. 相似文献
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比较研究了不同光强下生长的(透光率分别为12.5%、36%、50%、100%)两种入侵性不同的外来种——紫茎泽兰(Eupatorium adenophorum)和兰花菊三七(Gynura sp.)的生物量分配、叶片形态和生长特性。结果表明: 1)两种植物叶片形态对光环境的反应相似。弱光下比叶面积(SLA)、平均单叶面积(MLS)和叶面积比(LAR)较大,随着光强的升高,SLA、MLS、LAR和叶根比(LARMR)降低。2)100%光强下紫茎泽兰叶生物量比(LMR)、叶重分数(LMF)和叶面积指数高于低光强下的值,也高于兰花菊三七,支持结构生物量比(SBR)则相反。强光下紫茎泽兰叶片自遮荫严重,这可能是其表现入侵性的重要原因之一;兰花菊三七分枝较多,避免了叶片自遮荫,较多的分枝利于种子形成对其入侵有利。3)随生长环境光强的升高,两种植物的净同化速率(NAR)、相对生长速率(RGR)和生长对NAR的响应系数均升高(但100%光强下兰花菊三七RGR降低),平均叶面积比(LARm)和生长对LARm的响应系数均降低,但不同光强下LARm对生长的影响始终大于NAR。4)随着光强的减弱,两种植物都增加高度以截获更多光能,但它们的生物量分配策略不同,紫茎泽兰根生物量比(RMR)降低,SBR增大,而兰花菊三七SBR降低,RMR增大。紫茎泽兰的生物量分配策略更好的反应了弱光环境中的资源变化情况。结论:紫茎泽兰对光环境的适应能力强于兰花菊三七。 相似文献
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目的:探讨姜黄素(curcumin,Cur)对胃癌细胞系HGC-27细胞增殖及侵袭力的影响以阐明Cur的抗瘤机制。方法:采用MTT法检测不同剂量(0、10、20、30、40?mol/L)及不同处理时间(24、48、72h)的姜黄素对HGC-27细胞增殖活性的影响;Transwell侵袭实验检测姜黄素对HGC-27细胞侵袭力的抑制作用。结果:1.MTT法显示姜黄素显著抑制HGC-27细胞增殖,20μmol/L,30μmol/L,40μmol/L姜黄素处理72小时其生长抑制率分别为24.63%,32.42%,76.43%,有明显的剂量及时间依赖性,不同剂量组之间及不同处理时间组之间比较有差异显著性(P<0.05)。2.Transwell侵袭实验显示姜黄素显著抑制HGC-27细胞的侵袭力,呈剂量和时间依赖性,各剂量组与对照组比较差异有显著性(P<0.05)。结论:姜黄素通过抑制HGC-27细胞增殖和侵袭行为起到抗肿瘤作用。 相似文献