Refinement of a cell line based artificial diet for rearing the parasitoid wasp, Trichogramma pretiosum

An artificial diet incorporating insect cells originally developed for Trichogramma australicum Girault (Hymenoptera: Trichogrammatidae) was successfully used to rear Trichogramma pretiosum Riley (Hymenoptera: Trichogrammatidae). To refine the diet, individual components were removed. Chicken egg yolk and the insect cells were identified as the most important components for T. pretiosum development. Their removal resulted in few pupae and no adults. Removal of Grace’s insect medium, a common component of artificial diets, was found to markedly improve the development of T. pretiosum, producing 60% larva to pupa transition and 19% pupa to adult transition. There was no significant difference in T. pretiosum development on diets in which milk powder, malt powder or infant formula were interchanged, despite differences in nutrient composition. The use of yeast extract resulted in significantly higher survival to the adult stage when compared with yeast hydrolysate enzymatic and a combination of yeast extract and yeast hydrolysate enzymatic. Comparison of four antimicrobial agents showed the antibacterial agent Gentamycin and the antifungal agent Nystatin had the least detrimental effect on T. pretiosum development. The use of insect cell line diets has the potential to simplify artificial diet production and significantly reduce T. pretiosum production costs in Australia compared to diets using insect hemolymph or the use of natural or factitious hosts.     

Life-table study of Anagrus atomus, an egg parasitoid of the green leafhopper Empoasca decipiens, at four different temperatures

The objective of our study was to assess thepotential of the egg parasitoid Anagrusatomus L. (Hymenoptera: Mymaridae) for controlof the greenhouse leafhopper Empoascadecipiens Paoli (Homoptera: Cicadellidae). Theegg-adult development time, survivorship andreproduction of A. atomus were evaluatedat four constant temperatures (16, 20, 24 and28°C). Developmental time ranged from33.6 days at 16°C to 13.3 days at 28°C. Based on a linear regression ofdevelopment rate on temperature the lowerthreshold was estimated at 8.39°C. Anagrus atomus required 263.2 degree-days tocomplete its development from egg to adult. Theegg-adult survival rate and the sex ratio weresignificantly lower at 28°C than at theother three temperatures tested. The intrinsicrate of increase (r _m) variedsignificantly between all four temperatures.The potential of A. atomus to attackdifferent host ages was additionallyinvestigated. Host eggs were parasitizedthroughout their development but rate ofparasitism was reduced in host eggs older thansix days. The number of eggs parasitized waspositively density dependent but the rate ofparasitism decreased with increasing hostdensity. A maximum rate of parasitism of 62.5%was recorded. The potential impact of the eggparasitoid on the population dynamics of E. decipiens is discussed.     

中国塔蚁属系统分类研究(膜翅目,蚁科)

记述中国塔蚁属Pyramica Roger昆虫26种,其中描述3新种,提出1个新组合Pyramica dayui(Xu).提供了各个种的地理分布,编制了25种工蚁分种检索表,台湾塔蚁P.formosa(Terayama,Lin & Wu)仅知蚁后.26个中国已知种是:多氏塔蚁P.doherti(Emery),平岛塔蚁P.hirashimai (Ogata),命运塔蚁P.1achesis Bolton,王氏塔蚁P.emeswangiBolton,节膜塔蚁P.membranifera(Emery),高作塔蚁P.takasago(Terayama,Lin＆Wu),弄巴塔蚁P.nongba sp.nov.,威氏塔蚁P.wilsoni Wang,日本塔蚁P.japonica(Ito),山地塔蚁P.formosimonticola(Terayama,Lin & Wu),典剑塔蚁P.benten(Terayama,Lin&Wu),细毛塔蚁P.leptothrix (Wheeler),高雅塔蚁P.elegantula(Terayama ＆ Kubota),哀牢山塔蚁P.ailaoshana sp.nov.,玛祖塔蚁P.mazu(Terayama,Lin & Wu),吉上塔蚁P.kichijo(Terayama,Lin & Wu),杨氏塔蚁P.yangi sp.nov.,中华塔蚁P.sinensis Wang,六节塔蚁P.hexamera(Brown),提西塔蚁P.tisiphone Bolton,大禹塔蚁P.dayui(Xu),犬齿塔蚁P.canina(Brown & Boisvert),邵氏塔蚁P.sauteri(Forel),温和塔蚁P.mitis Brown,截头塔蚁P.mutica(Brown),台湾塔蚁P.formosa (Terayama,Lin＆Wu).     

寄生榆童锤角叶蜂的姬蜂种类研究(膜翅目,姬蜂科)

报道2种寄生榆童锤角叶蜂Agenocimbex elmina Li et Wu的姬蜂,其中1种为新种:天水镶颚姬蜂Hyposotertianshuiensis Sheng,sp.nov.;另一种为已知种:日本欧姬蜂Opheltes japonicus(Cushman,1924).     

片角叶蜂属研究及四新种记述(膜翅目,叶蜂科,平背叶蜂亚科)

对膜翅目叶蜂科平背叶蜂亚科的片角叶蜂属Indostegia Malaise进行了系统研究,描述了分布于中国川滇黔地区的4个新种:多齿片角叶蜂Indostegia multidenticula sp nov,短角片角叶蜂I brevicornis sp.nov,黑股片角叶蜂I.nigrofemorata sp nov.和短刃片角叶蜂I.breviserrula sp.nov..基于新的材料重新描述了片角叶蜂属的属征,编制了片角叶蜂属分种检索表.新种模式标本分别保存于中南林学院昆虫标本室和中国科学院动物研究所.     

中国小腹茧蜂亚科(膜翅目,茧蜂科)一新属新种记述

按Mason(1981)分类系统记述分布于福建坳头洪坑的小腹茧蜂亚科1新属1新种,即赵氏茧蜂属Chaoa gen.nov.和黄足赵氏茧蜂Chaoa flavipes sp.nov,模式标本存福建农业大学生物防治研究所.     

中国狭腹叶蜂属系统分类研究(膜翅目,叶蜂科)

厘订了中国狭腹叶蜂属种类,共报道14种,其中包括4新种:黄氏狭腹叶蜂Athlophorus huangae Wei et Nie,sp. nov.,亮翅狭腹叶蜂Athlophorus hyalopterus Wei et Nie,sp.nov.,烟翅狭腹叶蜂Athlophorus infuscatus Wei,sp.nov.,汪氏狭腹叶蜂Athlophorus wangae Nie et Wei,sp nov.,以及4个中国新纪录种:斑头狭腹叶蜂Athlophorus pilifrons(Cameron),黄褐狭腹叶蜂Athlophorus caprae Forsius,长斑狭腹叶蜂Athlophorus mimicarius Malaise和黑唇狭腹叶蜂Athlophorus gracilis(Konow).编制了中国种类检索表,新种模式标本分别保存于中南林学院昆虫标本室和中国科学院动物研究所.     

韩国黄蚜小蜂属分类研究(膜翅目:蚜小蜂科)

本文报道采自韩国的黄蚜小蜂属5种,包括1新种（Aphytis albus,sp．nov．)及4个韩国新记录种(Adiaspidis,A.japonicus,Aproclia和Avandenboschi),编制了雌成虫检索表。新种模式标本保存在韩国国立树木园和东北林业大学昆虫标本室。     

世界奇蚁属第三种记述(膜翅目:蚁科)

The third species of the ant genus Perissomyrmex in the world, P.fissus sp. nov., is collected from Ailao Mountain Nature Reserve of Yunnan Province. Perissomyrmex is a new record genus in China. Taxonomic key based on worker caste is provided for the 3 species: P.snyderi Smith, P monticola de Andrade, and P fissus sp. nov.