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31.
The effects of food and water deprivation on survival and reproduction of adult female German cockroaches were examined. Females, maintained under constant conditions, were deprived of food or water following adult maturation, mating, during the oothecal incubation period, and after first oothecal hatch. It was found that both food and water deprivations caused increased mortality, delays in the reproductive cycle and decreased oothecal hatch. The relative importance of food vs. water deprivation on reproduction is discussed along with possible reproductive strategies for this species.
Résumé Les femelles, maintenues en conditions constantes, ont été privées d'aliments et d'eau après la maturité imaginale, l'accouplement, pendant la période d'incubation et après l'éclosion de la première oothèque. On a observé une mortalité accure, un retard dans le cycle de reproduction et une diminution des éclosions de l'oothèque. L'importance relative de l'alimentation par rapport au jeûne hydrique sur la reproduction est discutée en relation avec les stratégies reproductives possibles de cette espèce.相似文献
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33.
A direct method is proposed for obtaining thermodynamic standard functions for native and denatured proteins using experimental data from scanning calorimetry, isothermal calorimetry and potentiometric titrations. The possibility of this approach is demonstrated on the example of lysozyme in the range of pH 1.5-7.0 and temperature 0-100 degrees C. Tests for the validity of the obtained functions of enthalpy and entropy are presented in the form of cyclic processes using experimental data obtained from thermodynamically different pathways. The Gibbs function is checked by comparison with results of an independent method. The methodic problems in determining and checking standard functions for proteins are discussed in detail. 相似文献
34.
Broad infectivity of Leidynema appendiculatum (Nematoda: Oxyurida: Thelastomatidae) parasite of the smokybrown cockroach Periplaneta fuliginosa (Blattodea: Blattidae) 下载免费PDF全文
Host specificity of parasites is important for the understanding of evolutionary strategies of parasitism that would be a basis of predictions of the disease expansion when parasitized hosts invade new environments. The nematode order Oxyurida is an interesting parasite group for studying the evolution of parasitism as it includes parasites of both invertebrates and vertebrates. In our survey, we found that the smokybrown cockroach Periplaneta fuliginosa was primarily infected with only one nematode species Leidynema appendiculatum. In two cases, L. appendiculatum was isolated from two additional cockroach species Pycnoscelus surinamensis, sold in Japan as a reptile food, and Blatta lateralis, captured in the field and cultured in the laboratory. Inoculation of L. appendiculatum into three additional cockroach species P. japonica, Blattella nipponica, and P. surinamensis also resulted in parasitism. Infection prevalence was high, and timing of postembryonic development from hatched nematode larva to mature adult in these hosts was identical with that in P. fuliginosa. While ecological interactions strongly determine the host range, such broad infectivity is still possible in this parasitic nematode. 相似文献
35.
Pioneer axons in insect legs are experimentally accessible model systems for the molecular identification and cellular localization of guidance cues regulating the path of axon growth. A detailed study of the Fe2 pioneer axons in the legs of the cockroach was performed to examine the diversity of guidance mechanisms. A detailed microscopic analysis of the axons at various points in their trajectory indicates that the Fe2 axons grow on a mesodermal substratum which contains the cues guiding their growth along a stereotyped path. An identified pair of muscle pioneer cells (MPC) are likely to play an important role in enabling the Fe2 growth cones to respond to mesodermal guidance cues. The addition of heparan sulfate, heparitinase, and phosphatidylinositol-specific phospholipase C to the medium perturbs thein situpath of growth of the Fe2 axons and the location of the MPC in cultured embryos. This indicates a role for heparan sulfate proteoglycans and glycosylphosphatidylinositol-anchored proteins in axon guidance. When these results are compared to those of similar experiments performed on the well-characterized Ti1 axons, they indicate significant differences in the mechanisms that are used for axon guidance. The Fe2 neurons are a good model for elucidating the mechanisms used to guide axon growth on nonmuscle mesodermal substrates often encountered in the periphery of vertebrate embryos. 相似文献
36.
E N Elpidina K S Vinokurov V A Gromenko Y A Rudenskaya Y E Dunaevsky D P Zhuzhikov 《Archives of insect biochemistry and physiology》2001,48(4):206-216
Compartmentalization of proteinases, amylases, and pH in the midgut of Nauphoeta cinerea Oliv. (Blattoptera:Blaberidae) was studied in order to understand the organization of protein and starch digestion. Total proteolytic activity measured with azocasein was maximal at pH 11.5 both in anterior (AM) and posterior (PM) halves of the midgut, but the bulk of activity (67%) was found in PM. Total AM and PM preparations were fractionated on a Sephadex G-50 column and further analysed by means of activity electrophoresis and specific inhibitors and activators. The major activity in PM was classified as an unusual SH-dependent proteinase with M(r) 24,000 and pH optimum with synthetic substrate BApNA at 10.0. The enzyme was 43-fold activated in the presence of 1 mM DTT, insensitive to synthetic inhibitors of serine (PMSF, TLCK, TPCK) and cysteine (IAA, E-64) proteinases, strongly inhibited by STI, and displayed four active bands on zymograms. In PM, activities of trypsin-like, chymotrypsin-like, subtilisin-like, and cysteine proteinases were observed. Aspartic and metalloproteinases were not detected. In AM, activity of unusual SH-dependent proteinase also dominated and activity of chymotrypsin-like proteinase was observed, but their levels were much lower than in PM. Distribution of amylase activity, exhibiting an optimum at pH 6.0, was quite the opposite. The major part of it (67%) was located in AM. Treatment of amylase preparation with proteinases from AM and PM reduced amylase activity twofold. pH of the midgut contents was 6.0-7.2 in AM, 6.4-7.6 in the first and 8.8-9.3 in the second halves of PM. Thus, pH in AM is in good agreement with the optimal pH of amylase, located in this compartment, but the activity of proteinases, including the ability to degrade amylase, in such an environment is low. Active proteolysis takes place in the second half of PM, where pH of the gut is close to the optimal pH of proteinases. 相似文献
37.
Food and the spatial distribution of adult female pinworms parasitic in the hindgut of Periplaneta americana L. International Journal for Parasitology 4: 759–771. The spatial distribution of the two pinworms Hammerschmidtiella diesingi and Leidynema appendiculata in the adult cockroach Periplaneta americana was determined by examination of hind guts fixed by immersion in liquid air. The position of the mouths of the worms reveals that L. appendiculata favours a more anterior position and is more restricted in its distribution than H. diesingi. The position of each species in the colon is unaffected by the presence of the other species or by crowding. Moreover, the two species are segregated in terms of their radial distribution, with the mouth of H, diesingi being restricted to a position near the intima of the colon while that of L. appendiculata favours the lumen. The two nematodes differ in the structure of the stoma and pharynx which reflect a difference in their diet. The food of L. appendiculata consists of relatively large particles while that of H. diesingi is finely patticulate. The ability of these two species to coexist in the same host is explained by their longitudinal and radial distribution and by the different sources of their food. 相似文献
38.
Ulrich Claussen Wanda Zevenboom Uwe Brockmann Dilek Topcu Peter Bot 《Hydrobiologia》2009,629(1):49-58
Eutrophication (nutrient enrichment and subsequent processes) and its adverse ecosystem effects have been discussed as main
issues over the last 20 years in international conferences and conventions for the protection of the marine environment such
as the North Sea Conferences and the 1992 OSPAR Convention (OSPAR; which combined and updated the 1972 Oslo Convention on
dumping waste at the sea and the 1974 Paris Convention on land-based sources of marine pollution). OSPAR committed itself
to reduce phosphorus and nitrogen inputs (in the order of 50% compared with 1985) into the marine areas and ‘to combat eutrophication
to achieve, by the year 2010, a healthy marine environment where eutrophication does not occur’. Within OSPAR, the Comprehensive
Procedure (COMPP) has been developed and used to assess the eutrophication status of the OSPAR maritime area in an harmonised
way. This is based on classification in terms of the following types of areas Non-Problem Areas (no effects), Potential Problem
Areas (not enough data to assess effects) and Problem Areas (effects due to elevated nutrients and/or due to transboundary
transport from adjacent areas). The COMPP consists of a set of harmonised assessment criteria with their area-specific assessment
levels and an integrated area classification approach. The criteria cover all aspects of nutrient enrichment (nutrient inputs,
concentrations and ratios) as well as possible direct effects (e.g. increased levels of nuisance and/or toxic phytoplankton
species, shifts and/or losses of submerged aquatic vegetation) and indirect effects (e.g. oxygen deficiency, changes and/or
death of benthos, death of fish, algal toxins). The COMPP also includes supporting environmental factors. It takes account
of synergies and harmonisation with the EC Water Framework Directive, and has formed a major basis for the EC eutrophication
guidance. Recently, additional components, such as total nitrogen, total phosphorus and transboundary transports have been
included in the assessment of, e.g. the German Bight. The second application of the COMPP resulting in an update of the eutrophication
status of the OSPAR maritime area will be finalised in 2008, and will include the agreed integrated set of Ecological Quality
Objectives (EcoQOs) with respect to eutrophication.
Guest editors: J. H. Andersen & D. J. Conley
Eutrophication in Coastal Ecosystems: Selected papers from the Second International Symposium on Research and Management of
Eutrophication in Coastal Ecosystems, 20–23 June 2006, Nyborg, Denmark 相似文献
39.
Our previous study [Kou et al., 2008. Juvenile hormone levels are increased in winners of cockroach fights. Horm. Behav. 52, 252–260] showed that the basic principle of the challenge hypothesis (hormone levels can respond to social stimuli to modulate aggression in vertebrates] could be applied to juvenile hormone (JH) levels and aggression in the lobster cockroach Nauphoeta cinerea. In that study, 80- to 85-day-old socially naïve males were used, as fighting is much more easily initiated in these older animals than in younger males, and JH III levels in the dominant were found to be significantly increased after an encounter compared to before the encounter and were significantly higher than those in the subordinates. In N. cinerea, newly emerged males usually show no aggressiveness towards each other and aggression is only initiated after several days of close contact. To investigate the development of aggression from an early age, in the present study, newly emerged males were paired to investigate the relationship between JH levels and aggression. The results showed that injection of JH III significantly increased the probability of the young males being fight winners. In each age group in which aggression was initiated, the dominants had significantly higher JH levels than either the subordinates or the same aged non-fighters. JH injection of subordinates on the day of rank establishment had no effect on the probability of rank switch. These results indicate that, (i) in newly emerged male pairs, JH plays a decisive role in rank establishment and the fact that dominant status is significantly associated with a higher JH titer and subordinate status with a lower JH titer is consistent with the basic principle of the challenge hypothesis, and (ii) after rank establishment, the lack of effect of JH treatment on rank change is consistent with the idea of “social inertia” in vertebrates. 相似文献
40.
Here we discuss the duration and position of Upper Rotliegend and Zechstein stratigraphic units in relation to the absolute time scale, and reinterpret a carbon isotope (δ13C) global event recorded from Late Permian (Lopingian/Guadalupian) marine deposits. Based on δ13C isotope correlation (chemostratigraphy) and of climatic evidence related to the end-Guadalupian global marine and terrestrial crisis, the Guadalupian/Lopingian boundary is proposed as the boundary between both the European Upper Rotliegend (URII)/Zechstein sediments and the parallel south-west USA Ochoan/Bell Canyon Formation units. The Zechstein deposition was strongly influenced by climatic oscillations, and the marine ingressions recorded in the North German Basin and North American Delaware Basin are presumed to have resulted from the same eustatic sea-level changes in western and northern coasts of the Northern Pangaea Supercontinent. Existing constraints on the age of the Upper Rotliegend II (UR II) deposits are imposed by the uncertainty of the chronostratigraphic boundary of the Kupferschiefer and by a time marker that is Illawarra, the boundary of the reversed polarity megachron (Kiaman) and mixed polarity megachron. Three options (A, B, C) have been discussed, which are connected with a time span comprising deposition of the UR II rocks, assuming that the time span needed for the Dethlingen/Lower Noteć formations and Hannover/Upper Noteć formations is about 6 myr. The time left for the deposition of the Parchim/Lower Drawa, Mirow/Upper Drawa deposits and the time hidden in the erosional gaps and hiatuses range from 1.6 myr to 4 myr or even 8 myr. These were based on the time interval related to the Kiaman Reversed Polarity megachron, which can contain more transient normal polarity zones than currently accepted. The presence and absolute dating of all such magnetozones is difficult to determine because they are represented in continental strata characterized by numerous, poorly time-constrained erosional gaps. The proposed option C is provisionally integrated with magnetostratigraphic results and shows an alternative stratigraphical scheme for the Upper Rotliegend. This alternate Upper Rotliegend stratigraphy helps correlate rocks (deposited in dry arid climatic conditions) in the lower part of the Upper Rotliegend II of the Southern Permian Basin (Havel and Drawa subgroups) with similar rocks in the Delaware Basin (attributed to formations within the Leonardian Regional Series). 相似文献