Highly conserved antigen-presenting function of CD1d molecules

 The lack of polymorphism of nonclassical antigen-presenting molecules has led to the proposal that they may carry out some conserved and essential antigen-presenting function. Although this is a plausible hypothesis, the major histocompatibility complex has undergone dramatic expansions and contractions through evolution, and there is surprisingly little evidence for interspecies conservation of nonclassical class I molecules. The CD1d molecule, by contrast, shows an extremely high degree of functional conservation between mice and humans, with regard to its interaction with the relatively invariant TCRs that are expressed by NK T cells. This conservation for CD1d recognition is observed either in the absence of exogenous Ag or together with a lipoglycan antigen. The close functional and phenotypic conservation of NK T cells, in mammalian species separated by approximately 50 million years, suggests an essential role in the immune system for CD1d recognition by NK T cells.     

Determinants of orangutan density in the dryland forests of the Leuser Ecosystem

Orangutans are the only great ape in Asia. Since orangutan densities vary between habitat types within regions and within similar habitat types among regions, it is important to determine areas with high densities for their protection. In this paper we show that orangutan density in old-growth dryland forests in the Leuser Ecosystem, Sumatra is significantly related to the density of large strangling figs and topsoil pH. In addition, large fig density depends on topsoil pH. Provided that orangutans are present and no hunting or logging occur, topsoil pH seems a promising method for rapid assessment of potential orangutan density over large areas.     

Enhancement of forest canopy research,education, and conservation in the new millennium

Study of the forest canopy has reached a critical stage in its development from a young frontier area of study to a vibrant and coalescing field of investigation and communication. Many current environmental and social issues at global scales (e.g., environmental change, acid deposition, loss of biodiversity) are related directly to our knowledge of forest canopies. I present six activities that are needed to help the vibrant and growing field of forest canopy studies progress efficiently. Enabling canopy researchers to communicate with each other and with those outside our field is an important element to address these issues. The establishment of a graduate-level training program is also a high priority to generate and maintain a healthy discipline. Formal procedures to identify particular forest sites of critical concern should be initiated, and these should include communication of prioritized sites to conservation groups and policy makers. Instilling a sense of wonder and appreciation for organisms and interactions in non-scientists is another important avenue for forest canopy conservation.     

Population subdivision and gene flow among wild orangutans

Genetic variability among populations of orangutans from Borneo and Sumatra was assessed using seven SSR loci. Most SSR loci were highly polymorphic and their allele frequencies exhibited substantial variation across subpopulations. While significant genetic subdivision was observed among the island populations, genetic distance did not increase with geographic distance and sufficient gene flow persists to prevent marked genetic subdivision. Since it is unlikely that the Bornean Orangutans dispersed naturally among locations separated by such formidable geographic barriers, human assistance might already have altered their genetic structure. Our data suggests that there may be at least two subspecific clades of orangutans within Borneo while Central Kalimantan animals may have become more genetically related to animals in Sumatra due to human intervention.     

Maasai Socioeconomic Conditions: A Cross-Border Comparison

A single round household survey was conducted between October 1997 and May 1998. Information was collected on 1545 Maasai households in Kenya and Tanzania using a standardized questionnaire. These data represent the first large-scale, cross-border comparable survey of socioeconomic indicators for the Maasai. The structure of the study is similar to that of a natural experiment: one ethnic group living in two very different nation-states. The survey results describe a current snapshot of Maasai livelihoods. Living arrangements are described at both the household (olmarei) and multihousehold (enkang) level, together with changes in housing type. The roles of cultivation, transhumant migration, and employment are described, including a consideration of the prevalence of murranism (warriorhood). The implications of rising levels of sedentarization and cash crop production for Maasai economic diversification are explored. Participation by Maasai in the tourism industry is extremely low despite the proximity of Maasai to major international tourist destinations in East Africa. Levels of formal education show a marked sex bias in completed primary school education in both countries, with far fewer women than men having attended school.     

The listing and de-listing of invertebrate species for conservation in Australia

Formal listing of species for protection is a responsible action in conferring conservation priority, and must be undertaken as objectively as possible, and redundant entries that distract from priority need be removed. Diverse groups, such as invertebrates, pose problems for listing because criteria of rarity and threat are often confounded. Species de-listed as a result of recovery action are a special category, in which conservation investment has been made; the principle of recognising these as rehabilitated species with a formal duty of aftercare is discussed. Although the paper focuses on invertebrates in Australia, the principles have wider relevance in species-level conservation focus.     

极度濒危植物五针白皮松的保护遗传学研究

用随机扩增多态(RAPD)方法对极度濒危植物五针白皮松(Pinus squamata X.W.Li)的遗传多样性和居群遗传结构进行了研究。14个随机引物共获得93条RAPD谱带,其中6条为多态带,多态位点百分率仅为6．45％,遗传多样性极低。Shannon指数I和Nei指数h在种内也只有0．020和0．030,两个亚居群间(半阴坡亚居群与半阳坡亚居群)遗传分化程度不高,遗传分化系数Gst只有0．110,与大多数松科植物近似,居群每代迁移数为4．032。五针白皮松极低的遗传多样性可能是由于它在演化过程中遭受过严重的灾害,造成严重的瓶颈效应,丧失其大部分遗传变异。在随后的演化过程中由于遗传漂变、自交衰退等小种群现象,导致遗传多样性的进一步丧失。另外,阔叶树种对其生存的挤压和人类的干扰也是导致五针白皮松遗传多样性降低的因素之一。本文最后对该结果的保护生物学意义进行了评价。     

Early phosphorus nutrition,mycorrhizae development,dry matter partitioning and yield of maize

We conducted a field experiment to test the hypothesis that improved phosphorus nutrition occurs in maize plants with rapid arbuscular (AM) mycorrhizae development at early developmental stages and that this also is reflected in dry matter allocation and final yield. A split-split plot design was used with previous crop (Zea mays L.-maize and Brassica napus L.-canola), tillage practices (no-tillage or conventional tillage) and P fertilization (5 levels) as factors chosen to modify mycorrhizae development at early developmental stages of maize. Previous cropping with canola resulted in decreased shoot-P concentration and shoot growth of maize at early stages. No-tillage resulted in higher shoot-P concentration but lower shoot weight than conventional tillage. Greater shoot-P uptake was related to a rapid intraradical development of mycorrhizae (previous crop of maize) or rapid connection to a mycorrhizal mycelium network (no-tillage treatments). Maize yield and harvest index were lower after cropping with canola. The yield for conventional tillage was higher than that for no-tillage but the harvest index was lower. The hypothesis was supported at early stages of maize growth by the effect of previous crop but not by results of tillage, because an unknown factor reduced growth in the no-tillage system. The hypothesis was supported at maturity by increased biomass allocation to grain relative to total shoot weight in treatments with greater shoot-P concentration at early stages.     

Protocol for in vitro propagation of Excoecaria agallocha L., a medicinally important mangrove species

An in vitro propagation protocol has been developed for Excoecaria agallocha L. (Euphorbiaceae), a mangrove species. Nodal segments were used for axillary shoot proliferation. One shoot from each node of binodal explants was observed 3 weeks after inoculation. The best axillary sprouting was seen on a newly formulated medium containing BA, Zeatin and IBA in concentrations of 13.3 μM, 4.65 μM and 1.23 μM, respectively. The new medium, first used in this study, has a specific composition of major nutrients, MS micronutrients and iron compounds. Nodal segments from rooted cuttings and seedlings responded better than those of mature tree explants. Multiple shoot induction was complemented with efficient shoot elongation, and repeated subculture of binodal segments from axillary shoots resulted in 10–12 shoots per explant in 3 months. Rooting was achieved by growing shoots in the new medium with 0.23 μM IBA. Regenerated plants were successfully acclimatized to the natural environment, and about 85% of plantlets survived under ex vitro conditions. This is the first report of micropropagation in the genus Excoecaria and also in mangrove tree species. Received: 11 August 1997 / Revision received: 10 February 1998 / Accepted: 2 March 1998