Identification of Annexin A1 interacting proteins in chronic myeloid leukemia KCL22 cells

In the present study, we used a functional proteomic approach to identify Annexin A1 (Anxa1) interacting proteins in the Philadelphia‐positive KCL22 cell line. We focused on Anxa1 because it is one of the major proteins upregulated in imatinib‐sensitive KCL22S cells versus imatinib‐resistant KCL22R. Our proteomic strategy revealed 21 interactors. Bioinformatic analysis showed that most of these proteins are involved in cell death processes. Among the proteins identified, we studied the interaction of Anxa1 with two phosphatases, Shp1 and Shp2, which were recently identified as biomarkers of imatinib sensitivity in patients affected by chronic myeloid leukemia. Our data open new perspectives in the search for annexin‐mediated signaling pathways and may shed light on mechanisms of resistance to imatinib that are unrelated to Bcr‐Abl activity. All mass spectrometry data have been deposited in the ProteomeXchange with identifier PXD000030.     

应用基因芯片筛选慢性乙型肝炎相关差异性基因

目的：应用基因芯片技术筛选不同病毒载量的慢乙肝病人及健康人差异性表达的基因。方法：选用含有48000位点的人类表达谱cDNA基因芯片,筛选4例慢乙肝病人与2例健康人外周血差异性表达的基因。结果：与健康人相比,慢乙肝患者有838个差异表达的基因,其中高表达的基因有150个,低表达的基因有688个。结论：用表达谱基因芯片可有效地研究高、低病毒载量的慢乙肝患者间,以及它们与健康人之间基因表达的差异,通过进一步分析有望筛选出与慢性乙型肝炎相关的新基因靶点。     

早期肠内营养辅助治疗重症胰腺炎的临床疗效分析

目的：探讨早期肠内营养辅助治疗重症胰腺炎的临床效果。方法：选择2009年4月-2012年2月我院收治的重症胰腺炎患者共46例,随机分为实验组和对照组各23例,实验组在综合治疗的基础采用早期肠内营养治疗（EN）,而对照组在综合治疗的基础上采用全胃肠外营养治疗（TPN）,比较两组患者的血清总蛋白、白蛋白、尿素氮水平及血淋巴细胞百分比变化、血淀粉酶和尿淀粉酶恢复正常时间、住院时间及病死率,并比较两组治疗前及治疗后24、48及72小时的APACHEII评分。结果：治疗后,实验组患者的血清总蛋白、白蛋白、尿素氮水平及血淋巴细胞百分比变化均较对照组明显增高,差异有统计学意义（P〈0．05）,而血淀粉酶、尿淀粉酶恢复时间、病死率及住院时间分别均明显较对照组降低或缩短,差异有统计学意义（P〈0．05）。治疗24、48及72小时后,实验组APACHEII评分均较治疗前显著减低（P〈o．05）,而对照组治疗24、48h后APACHEⅡ评分与治疗前比较差异无统计学意义（P〉0．05）,治疗72h后APACHEII评分较治疗前显著减低（P〈O．05）。结论：采用早期肠内营养辅助治疗重症胰腺炎患者能有效保护肠黏膜屏障功能,改善患者的营养状况,增强患者的免疫力,并改善患者的预后。     

Interactions between enteroviruses and autophagy in vivo

Autophagy plays a protective role during many viral and bacterial infections. Predictably, evolution has led to several viruses developing mechanisms by which to evade the inhibitory effects of the pathway. However, one family of viruses, the picornaviruses, has gone one step further, by actively exploiting autophagy. Using mice in which Atg5 has been conditionally deleted in pancreatic acinar cells, we have studied the outcome of infection by coxsackievirus B3 (CVB3), a member of the enterovirus genus and picornavirus family. Two key findings emerged: disruption of autophagy (1) dramatically compromised virus replication in vivo, and (2) significantly limited pancreatic disease.     

VMP1 is a new player in the regulation of the autophagy-specific phosphatidylinositol 3-kinase complex activation

We have elucidated a novel mechanism through which the autophagy-specific class III phosphatidylinositol 3-kinase (PtdIns3K) complex can be recruited to the PAS in mammalian cells, through the interaction between BECN1 and the vacuole membrane protein 1 (VMP1), an integral autophagosomal membrane protein. This interaction involves the binding between the C-terminal 20 amino acids of the VMP1 hydrophilic domain, which we have named the VMP1 autophagy-related domain (VMP1-AtgD), and the BH3 domain of BECN1. The association between these two proteins allows the formation of the autophagy-specific PtdIns3K complex, which activity favors the generation of phosphatidylinositol-3-phosphate (PtdIns3P) and the subsequent association of the autophagy-related (ATG) proteins, including ATG16L1, with the phagophore membranes. Therefore, VMP1 regulates the PtdIns3K activity on the phagophore membrane through its interaction with BECN1. Our data provide a novel model describing one of the key steps in phagophore assembly site (PAS) formation and autophagy regulation, and positions VMP1 as a new interactor of the autophagy-specific PtdIns3K complex in mammalian cells.     

Stem cells: Insights into the secretome

Stem cells have been considered as possible therapeutic vehicles for different health related problems such as cardiovascular and neurodegenerative diseases and cancer. Secreted molecules are key mediators in cell–cell interactions and influence the cross talk with the surrounding tissues. There is strong evidence supporting that crucial cellular functions such as proliferation, differentiation, communication and migration are strictly regulated from the cell secretome. The investigation of stem cell secretome is accumulating continuously increasing interest given the potential use of these cells in regenerative medicine. The scope of the review is to report the main findings from the investigation of stem cell secretome by the use of contemporary proteomics methods and discuss the current status of research in the field. This article is part of a Special Issue entitled: An Updated Secretome.     

金双歧联合通腑合剂治疗急性胰腺炎的临床研究

【摘 要】 目的 观察金双歧联合通腑合剂治疗急性胰腺炎的临床效果。方法 120例胰腺炎患者,随机分为常规治疗组、通腑合剂组和联合治疗组,每组40例。采取不同治疗方法,分别观察3组患者的临床治疗效果、实验室指标改善情况以及住院时间等。结果 联合治疗组治疗效果明显优于常规治疗组及通腑合剂组（P<0.05）,未出现恶化病例,其腹痛、腹胀缓解时间及血淀粉酶（AMY）和C反应蛋白（CRP）恢复时间明显短于常规治疗组和通腑治疗组,差异均有统计学意义（P<0.05）。联合治疗组和通腑合剂组在血白细胞（WBC）和血脂肪酶（LPS）恢复上差异无统计学意义（P>0.05）,但均优于常规治疗组,差异有统计学意义（P<0.05）。结论 金双歧联合通腑合剂治疗急性胰腺炎有较好的临床效果,并能缩短住院时间。     

Assessment of the potential iridology for diagnosing kidney disease using wavelet analysis and neural networks

Alternative or complementary medicine emphasizes therapies that are claimed to improve quality of life, prevent disease, and address conditions that conventional medicine has limited success in curing. There are many techniques which are prevalent in many countries and these can cause harm if not scientifically evaluated. The objective of this paper is to validate the use of iridology to diagnose kidney abnormalities. Two subject groups were evaluated: one was 168 subjects free from kidney disease and the other was 172 subjects with chronic renal failure. The procedure to acquire, process and classify the iris images was designed in such a way that avoids any dependency on the iridologists by using wavelet analysis and Adaptive Neuro-Fuzzy Inference System. The results show a correct classification for both subjects with kidney problems and normal subjects of 82% and 93%, respectively. The proposed technique conducted on a systemic disease with ocular manifestations showed encouraging results. However, it is necessary to perform extensive studies with diseases that do not have ocular manifestations according to conventional medicine in order to validate iridology as a valid scientific technique.     

葶苈大枣泻肺汤加减对慢性心力衰竭患者尿液白蛋白/肌酐比值及炎症因子的影响

目的：探索葶苈大枣汤对慢性心力衰竭患者微量尿白蛋白及血浆炎症因子IL-6,C反应蛋白的影响。方法：70例诊断为慢性心力衰竭的患者（心功能Ⅱ~Ⅳ级）按心功能分级并随机分为中药＋心衰基础治疗组（治疗组）和心衰基础治疗对照组（对照组）,每组35例;在进行积极控制感染,改善心功能的基础上,对照组患者给予心衰的基础治疗（利尿剂＋β受体阻断剂＋ARB或ACEI）;治疗组在心衰基础治疗的基础上给予葶苈大枣汤加减进行治疗,每2周随访1次,观察周期为3月。治疗前后观察患者的心功能变化,尿微量白蛋白的变化,炎症因子IL-6,CRP的变化。结果：基线资料比较,两组患者无显著性差异;经3月的治疗后,治疗组患者心功能改善情况要好于对照组患者,P〈0.05;与对照组比较,葶苈大枣汤加减可显著降低慢性心力衰竭患者血浆IL-6,CRP水平,降低尿微量白蛋白水平,P均〈0.05,且与对照组比较,不良反应事件未见显著增加,P〉0.05。结论：对于慢性心力衰竭患者,在心衰基础治疗的基础上结合葶苈大枣汤加减治疗可显著提高对慢性心力衰竭患者的临床疗效,且安全性良好,值得临床推广。     

芪苈强心胶囊辅助治疗老年慢性心力衰竭的临床研究

目的：探讨芪苈强心胶囊辅助治疗老年慢性心力衰竭的临床疗效及其安全性。方法：选择我科2012年1月．6月收治的102例老年慢性心力衰竭患者为研究对象,并将其随机分为两组,对照组42例,给予西医常规治疗及地高辛0．125mg／d;观察组60例,在对照组基础上将地高辛用量改为O．0625mg／d,并予芪苈强心胶囊,每次4粒,每日3次12服,2个月为1个疗程,治疗3个疗程后观察和比较两组心功能的变化。结果：观察组和对照组的治疗总有效率分别为95．0．％和78．6％,观察组显著高于对照组,两组比较差异有统计学意义（P〈0．05）;观察组的平均住院时间明显短于对照组（P〈0．05）。治疗后,两组心输出量（cO）、每搏输出量（SV）、左室射血分数（LVEF）舒张晚期与舒张早期充盈速度比（～E）均较治疗前明显升高（P〈0．05）,而脑钠肽（BNP）水平较治疗前显著降低（P〈O．05）,且观察组CO、SV水平均明显高于对照组（P〈0．05）,BNP水平明显低于对照组（P〈0．05）。治疗过程中,对照组发生洋地黄中毒3例,观察组无不良事件发生。结论：芪苈强心胶囊辅助治疗老年慢性心力衰竭可有效改善其心功能,并减少地高辛用量和洋地黄中毒的发生,值得临床推广应用。