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11.
Kristel Van Steen Nadia Tahri Geert Molenberghs 《Biometrical journal. Biometrische Zeitschrift》2004,46(2):187-202
Until recently, the most common parametric approaches to study the combined effects of several genetic polymorphisms located within a gene or in a small genomic region are, at the genotype level, logistic regressions and at the haplotype level, haplotype analyses. An alternative modeling approach, based on the case/control principle, is to regard exposures (e.g., genetic data such as derived from Single Nucleotide Polymorphisms – SNPs) as random and disease status as fixed and to use a marginal multivariate model that accounts for inter‐relationships between exposures. One such model is the multivariate Dale model. This model is based on multiple logistic regressions. That is why the model, applied in a case/control setting, leads to straightforward interpretations that are similar to those drawn in a classical logistic modeling framework. (© 2004 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) 相似文献
12.
H. Tanida W. Hohenboken 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1987,75(1):157-164
Summary Methods for calculating the probability of detecting a carrier of a recessive gene by utilizing matings among related individuals are presented for single and litter bearing species. The confidence level for detection of heterozygosity depends upon: (1) the genetic relationship between mates, (2) the number of mates per male and the number of offspring per mate, (3) whether an estimate of recessive gene frequency before selection is available and (4) the magnitude of that frequency. Methods of computing probability of heterozygosity vs homozygosity utilizing Bayes theorem also are presented. In the conventional progeny test method, a sire initially is assumed heterozygous before calculations are made, but no prior information concerning his probable genotype is utilized. In the method using Bayes theorem, prior sources of information from relatives or from estimates of population allele frequency are utilized. This method gives the exact probability that a sire is not a carrier, given prior information and that he produces all normal offspring. These methods could be used in any sexually reproducing species to identify not only detrimental genes but beneficial genes as well. 相似文献
13.
In this note we outline some recent results on the development of a statistical testing methodology for inverse problems involving partial differential equation models. Applications to several problems from biology are presented. The statistical tests, which are in the spirit of analysis of variance (ANOVA), are based on asymptotic distributional results for estimators and residuals in a least squares approach.Research supported in part under grants NSF MCS 8504316, NASA NAG-1-517, and AFOSRF-49620-86-C-0111. Part of this research was carried out while the first author was a visiting scientist at the Institute for Computer Applications in Science and Engineering (ICASE), NASA Langley Research Center, Hampton, VA, which is operated under NASA contracts NASI-18107 and NASI-18605 相似文献
14.
A. G. M. Steerneman E. A. van der Meulen W. Schaafsma G. N. van Vark 《Human Evolution》1990,5(5):449-456
Research on human evolution and sexual dimorphism motivates an interesting test problem. In studying hominid phylogeny it
is of interest to test whether parallel evolution plays a role. With regard to sexual dimorphism it is of interest to known
whether the directions of sexual dimorphism in the populations being compared are the same. We show that testing these two
problems gives rise to the same type of hypothesis testing, viz. the problem of testing the hypothesis that the means of independent,
normally distributed random vectors with unit covariance matrices are situated on a straight line through the origin. A test
is proposed and applied to study the sexual dimorphism of 20 recent skull populations. In this example the hypothesis of equal
directions of sexual dimorphism is rejected. The classical theory of constructing multiple discriminant functions (canonical
variates) is adapted to the problem of comparing sexual dimorphisms. 相似文献
15.
A simple procedure is described for the determination of the photosensitizing potency of drugs, using three leukemic cell lines, two of lymphocytic origin, L1210 and P388 and one of erythroid type, Friend-745. The procedure allows one to investigate several aspects of the photosensitization properties of tested compounds such as cellular localization and direct (trypan blue exclusion) or delayed (clonogenicity) photomediated toxicities.The method was assessed using crude hematoporphyrin derivative (HPD) as well as dihematoporphyrin ether (DHE) or commercially available Photofrin II. Results were compared to those obtained with normal cells, e.g spleen lymphocytes and erythropoietic stem cells (CFU-e), and discussed in the light of the relative response of normal versus transformed cells.Abbreviations DHE
Dihematoporphyrin Ether
- FCS
Fetal Calf Serum
- HPD
Hematoporphyrin Derivative
- PDT
Photodynamic Therapy 相似文献
16.
Abstract Low concentrations (e.g. 2 × 10−6 M) of an imidazole derivative anti-fungal agent, miconazole, were lethal for the Gram-negative, facultative aerobic pathogen Neisseria gonorrhoeae grown either alone or in mixed culture with the yeast Candida albicans . Electron microscopic observation of Neisseria cells exposed to miconazole showed the presence of blebs in the outer wall and areas of separation between the wall and the cytoplasmic membrane. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of cell lysates did not reveal differences in major outer membrane proteins between the treated and the untreated cells of any one strain. Imidazole derivatives are frequently used in the treatment of candidiasis. Our in vitro results show that low concentrations of one of them, miconazole, can be bactericidal for N. gonorrhoeae , a bacterium that can colonise sites of the human body where Candida is often found. 相似文献
17.
18.
Results from three experiments on basic learning and transfer in rhesus monkeys (Macaca mulatta) are reported in which fully automated testing paradigms, afforded by the Language Research Center's Computerized Test System
(LRC-CTS), were employed. Performance levels for discrimination learning set, transfer index, and mediational-learning testing
were uniformly higher than was predicted from the literature, in contrast to previous reports of compromised learning under
similar conditions (automated apparatus, planimetric stimuli, spatial discontiguity between stimuli and response loci). Analyses
reveal relatively advanced learning set performance, transfer-index ratios, and positive transfer of learning even at stringent
criterion levels. Moreover, the data suggest that rhesus monkeys tested in these experiments exhibit mediational instead of
associative learning strategies, as do great apes and in contrast to previous reports of rhesus learning. We argue that the
LRC-CTS enhances learning by nonhuman primate subjects, obviating those factors, reported in the literature from experiments
in which manual or other automated systems were employed, that compromise learning. 相似文献
19.
Summary Phytoalexins accumulated in selected woody plants in response to microbial attack or stress are reviewed and listed with respect to their chemical structure and probable biogenetic origin. The host-pathogen systems from which they have been isolated are described. The review also considers the antimicrobial activity of the phytoalexins to the causal pathogens and other microorganisms. 相似文献
20.
Yoshihiro Toh Akiko Mizutani Fuminori Tokunaga Tatsushi Muta Sadaaki Iwanaga 《Cell and tissue research》1991,266(1):137-147
Summary The structure of hemocytes in the normal state and during blood coagulation, and the intracellular localization of three clotting factors and two antimicrobial factors were examined in the Japanese horseshoe crabTachypleus tridentatus. Two types of hemocytes were found in the circulating blood: non-granular and granular hemocytes. The latter contained numerous dense granules classed into two major types: L- and D-granules. The L-granules were larger (up to 1.5 m in diameter) and less electron-dense than the D-granules (less than 0.6 m in diameter). The L-granules contained three clotting factors and one antimicrobial factor, whereas the D-granules exclusively contained the other antimicrobial factor. After treatment with endotoxin, the L-granules were released more rapidly than the D-granules, although almost all granules were finally exocytosed. The granular hemocyte possessed a single Golgi complex; possible precursor granules of L-granules and D-granules contained tubular and condensed dense material, respectively. These data are discussed in relation to the self-defense mechanisms of the horseshoe crab. 相似文献