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11.
Nitrogen is the major growth-limiting nutrient for marine algae. One potential source of nitrogen for marine algae is ammonium released by invertebrates. Many mid-intertidal reefs in northeastern New Zealand are dominated by a close association between the honeycomb barnacle Chamaesipho columna and an encusting brown alga Pseudolithoderma sp. Growth of Pseudolithoderma was enhanced in the presence of live C. columna, which released ammonium at a greater rate than the maximum rate of ammonium uptake by Pseudolithoderma. Algal tissue on barnacle tests had a lower C:N ratio than tissue located more than 2 cm from the nearest barnacle, suggesting the barnacle is an important source of nitrogen for the alga. The role of nutrient exchange in determining ecological patterns of species in marine communities is discussed. 相似文献
12.
We assessed the extent to which plants can acquire amino acids when supplied as single N-sources or when plants have access to a mixture of amino- and inorganic N sources. Because the uptake of different N-sources is temperature-dependent, the effects of temperature on amino-N uptake were also tested. Lolium perenne (perennial rye-grass) was grown hydroponically at 11 °C or 21 °C. Uptake of N was determined using 15N tracers at the growth temperature from solutions containing either nitrate, ammonium or glycine as single N sources and from a mixture containing all three N-forms. Estimates of the relative importance of amino acids such as glycine to the total N budget of plants will have been underestimated in studies where uptake was determined in single source solutions compared with those from solutions containing a mixture of N-forms. The proportion of total N acquired from the mixed N source as ammonium increased as temperature was reduced. Regarding the uptake and initial metabolism of glycine, uptake was probably the rate limiting step at 11 °C whilst it was the metabolism of glycine to serine at 21 °C. Although 15N incorporation into the plant amino-N pool was generally in proportion to the abundance of individual amino acids, its incorporation into the glycine pool was sometimes significantly less than predicted. 相似文献
13.
Concentration and flux of solutes from snow and forest floor during snowmelt in the West-Central Adirondack region of New York 总被引:7,自引:7,他引:0
Decreases in pH and increases in the concentration of Al and NO
3
–
have been observed in surface waters draining acid-sensitive regions in the northeastern U.S. during spring snowmelt. To assess the source of this acidity, we evaluated solute concentrations in snowpack, and in meltwater collected from snow and forest floor lysimeters in the west-central Adirondack Mountains of New York during the spring snowmelt period, 29 March through 15 April 1984.During the initial phase of snowmelt, ions were preferentially leached from the snowpack resulting in elevated concentrations in snowmelt water (e.g. H+ = 140 eq.l–1; NO
4
2–
= 123 eq.l–1; SO
3
–
= 160 eq.l–1). Solute concentrations decreased dramatically within a few days of the initial melt (< 50 eq.l–1). The concentrations of SO
4
2–
and NO
3
–
in snowpack and snowmelt water were similar, whereas NO–
3 in the forest floor leachate was at least two times the concentration of SO
4
2–
.Study results suggest that the forest floor was a sink for snowmelt inputs of alkalinity, and a net source of H+, NO
3
–
, dissolved organic carbon, K+ and Al inputs to the mineral soil. The forest floor was relatively conservative with respect to snowmelt inputs of Ca2+, SO
4
2–
and Cl–. These results indicate that mineralization of N, followed by nitrification in the forest floor may be an important process contributing to elevated concentrations of H+ and NO
3
–
in streams during the snowmelt period. 相似文献
14.
15.
The nitrate reductase (NR, EC 1.6.6.1) activity in root nodules formed by hydrogenase positive (Hup+ ) and hydrogenase negative (Hup− ) Rhizobium leguminosarum strains was examined in symbioses with the pea cultivar Alaska ( Pisum sativum L.), Rates of activity were determined by the in vivo assay in nodules from plants that were only N2 -dependent or grown in the presence of 2 m M KNO3 . The rates varied widely among strains, regardless of the Hup phenotype of the R. leguminosarum strain used for inoculation, but the overall results indicated that nodules formed by Hup− strains accumulated more nitrite in the incubation medium than did those with Hup− phenotypes. Total plant dry weight and reduced nitrogen content of pea plants grown in the presence of 2 m M KNO3 and inoculated with single Hup+ and Hup− R. leguminosarum strains were statistically different among some strains. These observations suggest that the possible advantages derived from the presence of the Hup system on whole plant growth may be counteracted by the higher rates of NR activity in the Hup− strains in the R. leguminosarum -pea symbiosis. 相似文献
16.
Published data on, and hypotheses regarding the effect of NO− 3 on functioning of legume root nodules are reviewed. It is concluded that a short-term reversible effect of NO− 3 may act via an increased resistance to O2 diffusion in nodules; this is coupled to decreased bacteroid respiration. For longer exposures to NO− 3 nodule activity is irreversibly lost, but how this relates to carbohydrate deprivation or NO-2 accumulation is unclear. Complicating factors include denitrification reactions and the interaction of NO− 2 with leghaemoglobin. 相似文献
17.
P. lanceolata andP. major were grown in culture solutions with nitrate or ammonium as the nitrogen source. Dry matter accumulation in the shoot was
faster with nitrate than with ammonium, whilst that of the roots was not affected by the nitrogen source. As a consequence,
the shoot-to-root ratio was lower with ammonium than with nitrate. InP. lanceolata, dry matter percentage of shoot and root tissue was lower with nitrate nutrition, suggesting better elongation growth than
with ammonium. However, in shoot tissue ofP. major the opposite was found. The rate of root respiration declined with time, and this was almost completely due to a declining
activity of the alternative path, which amounted to about 30–60% of total root respiration. Respiration via the cytochrome
path was for a part of time slightly increased by ammonium, whereas the activity of the alternative path was strongly enhanced.
The concentration of ethanol-soluble carbohydrates (SC) in the roots of both species was higher when nitrate was used, but
no difference in the concentration of starch was found. When the plants were transferred from one nitrogen source to the other,
many parameters, including the concentration of nitrate and chloride, and the shoot to root ratio, adjusted to the new situation
in both species.
Grassland Species Research Group, Publication no. 116. 相似文献
18.
A sand-culture experiment was conducted to study the influence of a deficiency of and an excess of micronutrients on the uptake
and assimilation of NH
4
+
and NO
3
−
ions by maize. By studying the fate of15N supplied as15NH4NO3 or NH4
15NO3, it was demonstrated that in maize plants NH4−N was absorbed in preference to NO
3
−
−N. The uptake and distribution of N originating from both NH
4
+
and NO
3
−
was considerably modified by deficiency of, or an excess of, micronutrients in the growth medium. The translocation of NH
4
+
−N from roots to shoots was relatively less than that of NO
3
−
−N. Deficiency as well as excessive amounts of micronutrients, in the growth medium, substantially reduced the translocation
of absorbed N into protein. This effect was more pronounced in the case of N supplied as NO
3
−
. Amino-N was the predominant non-protein fraction in which N from both NH
4
+
and NO
3
−
tended to accumulate. The next important non-protein fractions were NO
3
−
−N when N was supplied as NO
3
−
and amide-N when NH
4
+
was the source. The relative accumulation of15N into different protein fractions was also a function of imposed micronutrient levels. 相似文献
19.
Growth of 2-month-old nonnodulatedHippophaë rhamnoides seedlings supplied with combined N was compared with that of nodulated seedlings grown on zero N. Plant growth was significantly better with combined N than with N2 fixation and, although not statistically significant for individual harvests, tended to be highest in the presence of NH 4 + , a mixture of NH 4 + and NO 3 ? producing the highest yields. Growth was severely reduced when solely dependent on N2 fixation and, unlike the combined-N plants, shoot to root ratios had only slightly increased after an initial decrease. An apparently insufficient nodule mass (nodule weight ratio <5 per cent) during the greater part of the experimental period is suggested as the main cause of the growth reduction in N2-fixing plants. Thein vivo nitrate reductase activity (NRA) of NO 3 ? dependent plants was almost entirely located in the roots. However, when grown with a combination of NO 3 ? and NH 4 + , root NRA was decreased by approximately 85 per cent.H. rhamnoides demonstrated in the mixed supply a strong preference for uptake of N as NH 4 + , NO 3 ? contributing only for approximately 20 per cent to the total N assimilation. Specific rates of N acquisition and ion uptake were generally highest in NO 3 ? +NH 4 + plants. The generation of organic anions per unit total plant dry weight was approximately 40 per cent less in the NH 4 + plants than in the NO 3 ? plants. Measured extrusions of H+ or OH? (HCO 3 ? ) were generally in good agreement with calculated values on the basis of plant composition, and the acidity generated with N2 fixation amounted to 0.45–0.55 meq H+. (mmol Norg)?1. Without acidity control and in the presence of NH 4 + , specific rates of ion uptake and carboxylate generation were strongly depressed and growth was reduced by 30–35 per cent. Growth of nonnodulatedH. rhamnoides plants ceased at the lower pH limit of 3.1–3.2 and deterioration set in; in the case of N2-fixing plants the nutrient solution pH stabilized at a value of 3.8–3.9 without any apparent adverse effects upon plant performance. The chemical composition of experimental and field-growing plants is being compared and some comments are made on the nitrogen supply characteristics of their natural sites. 相似文献
20.
Activity of key nitrogen assimilating enzymes was studied in developing grains of high-lysine opaque sorghum P-721 and normal
sorghum CSV-5. The higher percentage of protein in opaque sorghum was mainly due to lower starch content since protein per
grain was less than in CSV-5. During grain development, albufn and globulin decreased while prolafne and glutelin increased.
Prolafne content in CSV-5 was higher than in opaque sorghum. Average nitrate reductase activity in flag and long leaf were
similar in both the varieties. The nitrate reductase activity decreased during grain development. Glutamate dehydrogenase
activity was higher during early development and lower at later stages in opaque sorghum than in CSV-5. Glutamate oxaloacetate
transaminase activity was higher and glutamine synthetase lower in opaque sorghum than in CSV-5 grains during development.
Glutamate synthase activity was higher in opaque sorghum up to day 20 and lower thereafter than in CSV-5. It is suggested
that reduced activities of glutamine synthetase as well as glutamate synthase in opaque sorghum as compared to CSV-5 during
later stages of development may restrict protein accumulation in the former. 相似文献