扁刺蛾核型多角体病毒新分离株的基因组测序与分析

从自然死亡的扁刺蛾幼虫分离到一株核型多角体病毒。扁刺蛾核型多角体病毒新分离株(Oxyplax ochracea nucleopolyhedrovirus-Thosea sinensis,OxocNPV-Ts)具有典型的杆状病毒特征，呈单粒包埋型病毒粒子，病毒颗粒(Occlusion-derived virus,ODV)呈不规则的多边形，直径为0.63μm～1.49μm。OxocNPV-Ts能有效杀灭扁刺蛾幼虫，它对扁刺蛾幼虫的半致死浓度LC₅₀为4×10⁶ PIB/mL，是一种理想的生物防治剂。本实验对OxocNPV-Ts基因组进行了测序和鉴定。OxocNPV-Ts基因组全长114781 bp，包含124个开放阅读框。它与斜纹刺蛾核型多角体病毒(OxocNPV)关系最为密切，序列相似性高达99%。系统进化分析表明，OxocNPV-Ts属于杆状病毒科alpha杆状病毒属(Alphabaculovirus)，根据国际病毒分类委员会(International Committee on Taxonomy of Viruses,ICTV)的种属划分标...     

核型多角体病毒侵染及其与宿主免疫系统互作的研究进展

核型多角体病毒(Nucleopolyhedrovirus,NPV)应用广泛,已被开发成微生物杀虫剂和用于重组蛋白表达等.NPV具有两种病毒颗粒:包埋型病毒粒子(occlusion-derived virus,ODV)和芽生型病毒粒子(budded virus,BV),两者的构成和组装存在差异.病毒包涵体在肠道中溶解后释...     

Identification of the epitopes of monoclonal antibodies against P74 of Helicoverpa armigera nucleopolyhedrovirus

P74 is a per os infectivity factor of baculovirus. Here, we report the production of three monoclonal antibodies （mAbs）, denoted as 20D9, 20F9 and 21E1, raised against P74 of Helicoverpa armigera nucleopolyhedrovirus （HearNPV）, and the identification of their recognition epitopes. The full-length P74, without the transmembrane domains at the C-terminus, was first divided into three segments （N, M and C, respectively）, based on the proposed cleavage model for the protein, which were then expressed individually. Western blot analyses revealed specific cross-reactions with the N fragment, for both 20D9 and 21E1. Extensive truncation, followed by prokaryotic expression, of the P74 N fragment was then performed in order to screen for linear epitopes of P74. The recognition regions of 20D9 and 21E1 were revealed to be localized at R144-T153 and T199-C219, respectively. In addition, immunofluorescence microscopy indicated that 20D9 and 20F9 could recognize native P74 in HearNPV-infected cells. These findings will facilitate further investigations of the proteolytic processing of HearNPV P74, and of its involvement in virus-host interactions.