普外科手术后期并发脑梗死患者的相关危险因素病例对照研究

目的:探讨普外科手术后期患者并发脑梗死与其相关危险因素的关系,以指导普外科医生在术前和术中有效评估病人发生手术后期脑梗死的风险,并做到积极预防。方法:回顾性分析我院2009年~2014年普外科手术后期发生脑梗死的患者,采用病例对照研究的方法,将手术后发生脑梗死的患者36例作为病例组,按手术方式进行1:2配比,从相同手术方式且未发生脑梗死的的患者中随机抽取72例作为对照,采用SPSS13.0软件进行单因素和多因素条件Logistic回归分析。结果:单、多因素条件Logistic回归分析表明,高血压、糖尿病、血脂异常,术中低灌注均可能是普外科手术后期发生脑梗死的危险因素,多因素Logistic回归分析显示,在调整年龄和性别后,术中低灌注仍最容易发生脑梗死,其次为糖尿病和高血压病史。结论:普外科术中应注意血压的监测和控制,尽量避免或减少低灌注;术前良好控制血压、血脂、血糖有助于降低普外科手术后患者发生脑梗死的风险;普外科医生应根据手术患者危险因素的多少及严重程度,尤其对于高龄患者,选择合理的手术时机及手术方式可能归避手术后期脑梗死的发生。     

Microbial diversity in coastal sediments during pre- and post-tsunami periods in the south east coast of India

Sediment samples were collected from 12 beaches affected by the 2004 Asian Tsunami in the south-east coast of India between Vanagiri and Nagoor. The objective of the present study is to delineate the microbial diversity in pre- and post-tsunami disaster coastal sediments. The collected marine sediments indicate that the overall microbial diversity is higher in the pre-tsunami sediments. The increase in pathogenic bacteria and fungal species after the tsunami is obscured due to inundation and backwashing of seawater along the coast. The reduction of other microbial diversity after the tsunami is attributed that the coastal and shelf sediments play an important role in the demineralization of organic matter, which supports the growth of microbes. The continuous exchange of ocean water and backwashing of coastal sediments by the tsunami wave probably reduced the pathogenic bacterial diversity in the sediments.     

犬繁殖调控技术研究进展

由于犬具有一些独特的生殖生理特点,使犬的繁殖调控技术的发展一直面临着一些困难。目前犬繁殖调控技术的研究远远落后于其他哺乳动物。本文综述了犬诱导发情、精液保存、人工受精、胚胎移植及克隆等犬的繁殖调控技术国内外研究进展。     

胚胎工程中激素选择的生理学解析

从生理学的角度解析了家畜发情机制及胚胎工程中用孕激素进行同期发情处理和用促性腺激素进行超数排卵的原因.     

Expression in Escherichia coli of Three Different Soybean Late Embryogenesis Abundant （LEA） Genes to Investigate Enhanced Stress Tolerance

In order to identify the function of late embryogenesis abundant (LEA) genes, in vitro functional analyses were performed using an Escherichia coli heterologous expression system. Three soybean late embryogenesis abundant (LEA) genes, PMll (GenBank accession No. AF004805; group 1), PM30(AF117884; group 3), and ZLDE-2 (AY351918; group 2), were cloned and expressed in a pET-28a system.The gene products were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and identified by mass spectrometry. E. coli cells containing the recombinant plasmids or empty vector as controls were treated by salt and low temperature stress. Compared with control cells, the E. coli cells expressing either PMll or PM30 showed a shorter lag period and improved growth when transferred to LB (Luria-Bertani) liquid media containing 800 mmol/L NaC1 or 700 mmol/L KC1 or after 4℃ treatment. E. coli cells expressing ZLDE-2 did not show obvious growth improvement both in either high KC1 medium or after 4℃ treatment. The results indicate that the E. coli expression system is a simple, useful method to identify the functions of some stress-tolerant genes from plants.     

酿酒酵母对数生长后期代谢重构的全基因组表达谱芯片分析

为了探讨酵母进入对数生长后期以后酒精生产速度降低的原因, 我们利用酵母表达谱芯片技术对酿酒酵母细胞从对数生长中期进入对数生长后期时的全基因组表达谱进行了分析, 发现酵母在对数生长中期的表达谱非常稳定, 而一旦进入对数生长后期, 则出现明显的代谢重构现象。许多氨基酸合成和代谢相关的基因、离子转移以及与能量的生成和储存等功能相关的基因出现了不同程度的上调; 而许多涉及酵母转座和DNA重组的基因则表达下调; 一些中心代谢途径也发生了代谢重构, 包括: 琥珀酸和a-酮戊二酸生成途径基因的一致上调, 都与氨基酸合成和代谢相关基因表达的结果相吻合。结果表明: 由于氨基酸合成的需求量增加, 进入对数生长后期酵母的代谢转向TCA循环和乙醛酸循环, 导致酒精的生产速率降低。     

圈养条件下狍发情配种模式的研究

通过在吉林农业科技学院左家校区动物实习基地进行试验,研究了野生狍(中国亚种)在人工驯化饲养条件下的发情配种模式.研究结果表明:狍属于短日照繁殖动物,发情和配种在秋季进行,每年有一个发情周期,性成熟是14±2月龄;雄狍在整个发情配种期始终保持兴奋状态,随时可以与达到发情旺期的雌狍进行交配,发情的雌狍外观没有明显表现,当对雄狍表现好感、主动亲近雄狍时,就是交配的最好时机;狍配种方式采用单雄群雌的同期复配方式,性别比例为雄性狍∶雌性狍=1∶4.     

Regulation of cellular adhesion molecule expression in murine oocytes,peri-implantation and post-implantation embryos

Expression of the adhesion molecules, ICAM-1, VCAM-1, NCAM, CD44, CD49d (VLA-4, α chain) ,and CDlla (LFA-1, α chain) on mouse oocytes, and pre- and peri-implantation stage embryos was exam-ined by quantitative indirect immunoliuorescence microscopy. ICAM-1 was most strongly expressed at the oocyte stage, gradually declining almost to undetectable levels by the expanded blastocyst stage. NCAM,also expressed maximally on the oocyte, declined to undetectable levels beyond the morula stage. On the other hand, CD44 declined from highest expression at the oocyte stage to show a second maximum at the compacted 8-cell/morula. This molecule exhibited high expression around contact areas between trophecto-derm and zona pellucida during blastocyst hatching. CD49d was highly expressed in the oocyte, remained significantly expressed throughout and after blastocyst hatching was expressed on the polar trophecto-derm. Like CD44, CD49d declined to undetectable levels at the blastocyst outgrowth stage. Expression of both VCAM-1 and CDlla was undetectable throughout. The diametrical temporal expression pattern of ICAM-1 and NCAM compared to CD44 and CD49d suggest that dynamic changes in expression of adhesion molecules may be important for interaction of the embryo with the maternal cellular environment as well as for continuing development and survival of the early embryo.     

糜蛋白酶治疗蝮蛇咬伤后期肿胀34例体会

蝮蛇咬伤是我县夏秋季节的一种常见病 ,许多患者未经正规处理、治疗 ,延误治疗时机 ,常导致后期肿胀 ,甚至肢体功能障碍。我所近年来采用糜蛋白酶治疗蝮蛇咬伤后期肿胀 3 4例。现报告如下。1　临床资料1 .1　一般资料　本组 3 4例中 ,男 1 6例 ,女 1 8例 ;年龄最小 6岁 ,最大 72岁 ,其中 6～ 40岁 1 9例 ,40岁以上 1 5例 ;大多咬伤暴露部位 ,其中上肢 1例 ,下肢 3 3例 ;咬伤月份 5～ 1 1月多见 ,1 2月～ 4月份较少 ;咬伤收治时间 5～ 1 0天 2 0例 ,1 0～ 3 6天 1 4例。1 .2　临床表现　患肢肿胀 ,呈凹陷性水肿 ,有瘀斑、精神紧张、轻度头晕…     

影响杜泊羊冷冻胚胎移植成功率的因素

采用引进的杜泊羊冷冻胚胎,以云南当地绵羊为受体进行胚胎移植。同期发情处理了158只受体羊,同期发情率为82．91％;对102只进行了胚胎移植,实际移植率为77．86％,3个情期内移植妊娠率达74．5％;出生68只,产羔率达66．7％。分析表明,胚胎发育阶段及级别、卵巢黄体情况、以及胚胎移植技术熟练程度直接影响胚胎移植成功率;此外,受体羊的处理程序及移植后的饲养管理、移植时机的把握、移植季节以及胚胎冷冻及解冻方法也会影响杜泊羊移植妊娠率,进而影响产羔率。