The importance of heparan sulfate in herpesvirus infection

Herpes simplex virus type-1 (HSV-1) is one of many pathogens that use the cell surface glycosaminoglycan heparan sulfate as a receptor.Heparan sulfate is highly expressed on the surface and extracellular matrix of virtually all cell types making it an ideal receptor.Heparan sulfate interacts with HSV-1 envelope glycoproteins gB and gC during the initial attachment step during HSV-1 entry.In addition,a modified form of heparan sulfate,known as 3-O-sulfated heparan sulfate,interacts with HSV-1 gD to induce fusion between the viral envelope and host cell membrane.The 3-O-sulfation of heparan sulfate is a rare modification which occurs during the biosynthesis of heparan sulfate that is carded out by a family of enzymes known as 3-O-sulfotransferases.Due to its involvement in multiple steps of the infection process,heparan sulfate has been a prime target for the development of agents to inhibit HSV entry.Understanding how heparan sulfate functions during HSV-1 infection may not only be critical for inhibiting infection by this virus,but it may also be crucial in the fight against many other pathogens as well.     

N-乙酰氨基葡萄糖转移酶V与肿瘤的关系

最近的研究认为，GnT—V在肿瘤发展及转移过程中是一个双功能蛋白质。GnT—V是一个高尔基体酶，但在某些肿瘤细胞中，GnT-V同类分子在高尔基体不能成簇，分子间二硫键介导的同类蛋白质寡聚体不能形成，GnT—V单体易受蛋白酶攻击，最终分泌到培养基中，分泌出细胞的GnT-V在生理浓度范围内能引起肿瘤血管生成。     

大剂量有机磷农药中毒1例报告

1临床资料患者,女,51岁。因与家人争吵后,自服甲胺磷农药约350ml,家人发现时患者已不省人事,约40min后送我院。入院查体:体温36.5℃,脉搏50次/分,呼吸30次/分,血压16.0/10.0kPa,神志不清,两侧瞳孔等大等圆,直径约1.5mm,对光反射迟钝。两肺可闻湿性罗音,心律齐,各瓣膜区未闻及杂音。腹部平软,肝脾肋下未触及,肠鸣音亢进。血常规:Hb127g/L,WBC9.6×109/L,N:89.6%,L:10.4%。血电解质,肝、肾功能均正常,血胆碱脂酶:13活力单位。入院后立即给予洗胃、给氧、导尿,建立静脉通道,并给予解磷定静推,阿托品微量泵持续泵入,维持阿托品化,甘露醇脱水…