5,7-双羟色胺损毁大鼠中缝背核后底丘脑核的神经活动增强

采用玻璃微电极在体细胞外记录法,观察了5,7-双羟色胺(5,7-dihydroxytryptamine,5,7-DHT)损毁大鼠中缝背核(dorsalraphenucleus,DRN)后,底丘脑核(subthalamicnucleus,STN)神经元电活动的变化。结果发现,对照组和DRN损毁组大鼠STN神经元的放电频率分别是(6.93±6.55)Hz和(11.27±9.31)Hz,DRN损毁组大鼠的放电频率显著高于对照组(P<0.01)。在对照组大鼠,13%的神经元呈现规则放电,46%为不规则放电,41%为爆发式放电;而在DRN损毁组大鼠,具有规则、不规则和爆发式放电的神经元比例分别为9%、14%和77%,爆发式放电的STN神经元比例明显高于对照组(P<0.01)。结果显示,DRN损毁后大鼠STN神经元的放电频率增高,爆发式放电增多,提示在正常大鼠DRN抑制STN神经元的活动。     

微电泳GABA和5—HT对大鼠丘脑束核单位痛放电的影响

本实验用多管微电极细胞外记录和离子微电泳方法，在水合氯醛麻醉的ＳＤ大鼠上观察了γ－氨基丁酸和５－羟色胺以及它们的受体阻断剂（印防已毒素和赛庚啶）对丘脑束旁核单位痛放电的影响。结果表明：（１）电泳ＧＡＢＡ可抑制Ｐｆ神经元的痛放电，这作用可被电泳印防已毒素所阻断，而单独电泳印防已毒素可加强Ｐｆ的痛放电。（２）电泳５－ＨＴ对Ｐｆ单位痛放电在有些单位表现加强作用，另一些单位表现抑制作用，仅前者可被电泳赛庚     

二十世纪五十年代后期中国率先发现大脑皮层-丘脑非特异投射区间闭合线路的活动

<正>大脑皮层-丘脑中部非特异投射区间存在闭合线路,它的活动在人的意识方面有重要作用,人入睡时它不活动,如果它受损,如丘脑中部梗死,人就会昏迷。在国外,这个系统于上世纪八十年代才被发现,九十年代后才逐步搞清楚[1-3],而在二十世纪五十年代后期,我们中国科学院上海生理研究所已经率先报告了这个闭合线路[4-6],只是由于当时此工作的论文只能在国内期刊发表,我们和国外同行没有交流,因此这个发现不为外国科学家所知。     

Noradrenaline as a putative neurotransmitter mediating hypotension—induced FOs—like immunoreactivity in the supraoptic nucleus of the rat

Hemorrhage or hypotension induces extensive Fos-like immunoreactivity in the magnocellular neurosecretory cells in the supraoptic nucleus of the hypothalamus in rat,especially in the vasopressin neurons.The present study was to explore the neurotransmitter mediating this effect,Microinfusion of the alpha-adrenergic blocker into the supraoptic nucleus reduced the hypotension-induced FOs.whereas beta-antagonist did not affect it significantly.Alaha1-and alpha2-antagonist,prazosin and yohimbine,both reduced the Fos-Positive cell counts.However,the effective dosage of yohimbine was much larger,Alpha1-agonist,methoxamine,induced abundant Fos-like immunoreactivity in the vasopressin cells in this nucleus,while beta-and alpha2-agonist did not elicit such effect.Administration of the noradrenergic re-uptake inhibitor desipramine,to this nucleus to locally accumulate the spontaneously released noradrenaline from the nerve terminals also induced Fos expression,mostly in the vasopressin cells.