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991.
Petr Baldrian Jana Vo?��?kov�� Petra Dobi��?ov�� V��ra Merhautov�� Ludmila Lis�� Vendula Val��?kov�� 《Plant and Soil》2011,338(1-2):111-125
Production of extracellular enzymes participating in the degradation of biopolymers was studied in 29 strains of nonbasidiomycetous microfungi isolated from Quercus petraea forest soil based on the frequency of occurrence. Most of the isolates were ascomycetes and belonged to the genera Acremonium, Alternaria, Cladosporium, Geomyces, Hypocrea, Myrothecium, Ochrocladosporium, and Penicillium (18 isolates), and two isolates were zygomycetes. Only six isolates showed phenol oxidation activity which was low and none of the strains were able to degrade humic acids. Approximately half of the strains were able to degrade cellulose and all but six degraded chitin. Most strains produced significant amounts of the cellulolytic enzymes cellobiohydrolase and ??-glucosidase and the chitinolytic enzymes chitinase, chitobiosidase, and N-acetylglucosaminidase. The highest cellulase activities were found in Penicillium strains, and the highest activity of chitinolytic enzymes was found in Acremonium sp. The production of the hemicellulose-degrading enzymes ??-galactosidase, ??-galactosidase, and ??-mannosidase was mostly low. The microfungal strains were able to produce significant growth on a range of 41?C87, out of 95 simple C-containing substrates tested in a Biolog? assay, monosaccharides being for all strains the most rapidly metabolized C-sources. Comparison with saprotrophic basidiomycetes from the same environment showed that microfungi have similar cellulolytic capabilities and higher chitinase activities which testifies for their active role in the decomposition of both lignocellulose and dead fungal biomass, important pools of soil carbon. 相似文献
992.
Martins LM Wanke B Lazéra Mdos S Trilles L Barbosa GG Macedo RC Cavalcanti Mdo A Eulálio KD Castro JA Silva AS Nascimento FF Gouveia VA Monte SJ 《Memórias do Instituto Oswaldo Cruz》2011,106(6):725-730
Throughout Brazil, Cryptococcus neoformans is the cause of cryptococcosis, whereas Cryptococcus gattii is endemic to the northern and northeastern states. In this study, the molecular types of 63 cryptococcal isolates recovered from the cerebrospinal fluid of meningitis patients diagnosed between 2008-2010 in Teresina, Piauí, Brazil, were analysed. Out of the 63 patients, 37 (58.7%) were human immunodeficiency virus (HIV)-positive and 26 (41.3%) were HIV-negative. URA5-restriction fragment length polymorphism analysis identified 37/63 (58.7%) isolates as the C. neoformans VNI genotype, predominantly in HIV-positive patients (32/37, 86.5%), and 24/63 (38.1%) as the C. gattii VGII genotype, mostly in HIV-negative patients (21/26, 80.8%). The occurrence of C. gattii VGII in six apparently healthy children and in seven adolescents/young adults in this region reaffirms the endemic occurrence of C. gattii VGII-induced primary cryptococcosis and early cryptococcal infection. Lethality occurred in 18/37 (48.6%) of the HIV-positive subjects and in 13/26 (50%) of the HIV-negative patients. Our results provide new information on the molecular epidemiology of C. neoformans and C. gattii in Brazilian endemic areas. 相似文献
993.
Ribosomal protein mutations in Arabidopsis (Arabidopsis thaliana) result in a range of specific developmental phenotypes. Why ribosomal protein mutants have specific phenotypes is not fully known, but such defects potentially result from ribosome insufficiency, ribosome heterogeneity, or extraribosomal functions of ribosomal proteins. Here, we report that ovule development is sensitive to the level of Ribosomal Protein L27a (RPL27a) and is disrupted by mutations in the two paralogs RPL27aC and RPL27aB. Mutations in RPL27aC result in high levels of female sterility, whereas mutations in RPL27aB have a significant but lesser effect on fertility. Progressive reduction in RPL27a function results in increasing sterility, indicating a dose-dependent relationship between RPL27a and female fertility. RPL27a levels in both the sporophyte and gametophyte affect female gametogenesis, with different developmental outcomes determined by the dose of RPL27a. These results demonstrate that RPL27aC and RPL27aB act redundantly and reveal a function for RPL27a in coordinating complex interactions between sporophyte and gametophyte during ovule development.Eukaryotic cytoplasmic ribosomes are comprised of two subunits, a large 60S and a small 40S subunit. The 60S subunit includes 25S or 28S, 5.8S, and 5S ribosomal RNA (rRNA) and approximately 47 ribosomal proteins, whereas the 40S subunit includes an 18S rRNA and approximately 33 ribosomal proteins. In plants and animals, reduced ribosomal protein function results in specific developmental phenotypes (Byrne, 2009; Warner and McIntosh, 2009; McCann and Baserga, 2013; Terzian and Box, 2013; Tsukaya et al., 2013). Currently, it is not known how ribosomal proteins modulate development. Potentially specific developmental phenotypes in ribosomal protein mutants are an outcome of ribosome haploinsufficiency and reduced global protein synthesis or reduced translation of specific proteins. Alternatively, ribosomal proteins, in addition to their role in translation, may have extraribosomal function required for specific developmental processes.In Arabidopsis (Arabidopsis thaliana), cytoplasmic ribosomal proteins are encoded by two to five genes (Barakat et al., 2001; Giavalisco et al., 2005; Carroll et al., 2008). Mutations in single ribosomal protein genes are sometimes gametophyte or embryo lethal (Weijers et al., 2001; Tzafrir et al., 2004). However, many ribosomal protein mutants are viable. These mutants typically display a subtle change in leaf shape and may also have distinct developmental defects affecting embryo morphogenesis, inflorescence development, the transition to flowering, and plant stature (Van Lijsebettens et al., 1994; Ito et al., 2000; Pinon et al., 2008; Yao et al., 2008; Byrne, 2009; Fujikura et al., 2009; Falcone Ferreyra et al., 2010; Rosado et al., 2010; Horiguchi et al., 2011; Szakonyi and Byrne, 2011a, 2011b; Stirnberg et al., 2012). Female fertility is also reduced in several ribosomal protein mutants. Mutations in the ribosomal protein genes SHORT VALVE1 (STV1)/RPL24B, SUPPRESSOR OF ACAULIS52 (SAC52)/RPL10A, ARABIDOPSIS MINUTE-LIKE1 (AML1)/RPS5B, and the Ribosomal Protein L27a gene RPL27aC reduce female fertility (Weijers et al., 2001; Nishimura et al., 2005; Imai et al., 2008; Szakonyi and Byrne, 2011b). aml1 and sac52-t1 are partially and fully gametophyte lethal, respectively. Although lower fertility in stv1 and rpl27ac is associated with defective ovules, the nature of the fertility defect in these mutants has not been fully explored.Female gametophyte development is also disrupted by mutations in a number of genes predicted to be involved in ribosome biogenesis. SLOW WALKER1 (SWA1), SWA3/Arabidopsis thaliana RNA HELICASE36 (AtRH36), and NUCLEOLAR FACTOR1 (NOF1) encode nucleolar-localized proteins required for processing 18S pre-rRNA (Shi et al., 2005; Harscoët et al., 2010; Huang et al., 2010; Liu et al., 2010). Mutations in other genes encoding proteins predicted to be involved in pre-rRNA processing and ribosome maturation or in export of preribosomes from the nucleus to the cytoplasm also reduce female fertility (Li et al., 2009, 2010; Chantha et al., 2010; Wang et al., 2012; Missbach et al., 2013). These mutants share similar phenotypes, where female gametophyte development is delayed and there is a failure in progression through gametophyte mitotic cell divisions. Transmission of these ribosome biogenesis mutants through the female is often reduced. This ostensibly reflects a requirement for active ribosome synthesis and sufficient ribosome levels to support morphogenesis of the gametophyte.Here, we show that mutations in a number of different ribosomal protein genes lead to reduced seed set and an increase in the number of defective ovules in siliques. This is particularly apparent in mutants affecting ribosomal protein RPL27a. We show the two RPL27a genes, RPL27aC and RPL27aB, act redundantly and that ovule development is sensitive to the dose of RPL27a. rpl27ac and rpl27ab mutations are together female and male gametophyte lethal. Single rpl27ac mutants also result in some female gametophyte lethality. In the homozygous rpl27ac-2 mutant, the mature embryo sac is frequently expelled from the ovule, suggesting RPL27a is necessary for maintaining a viable gametophyte. However, in the heterozygous rpl27ac-2/+, gametogenesis frequently fails early in development. This occurs independent of the genotype of the gametophyte, indicating somatic sporophyte cells in the mutant affect gametophyte development. Together, our data demonstrate that appropriate levels of RPL27a in the sporophyte and gametophyte are required for female gametophyte development and plant fertility. 相似文献
994.
Large hydroelectric dams are one of the current drivers of habitat loss across Amazonian forests. We investigated how the primate community at a hydroelectric dam in Brazilian Amazonia responded to changes in the landscape and local habitat structure of land‐bridge islands after 21 yr of post‐isolation history. The Balbina Dam, constructed in 1986, inundated 3129 km2 of primary forests and created more than 3500 variable‐sized islands. We conducted primate and habitat structure surveys on 20 islands from 5 to 1815 ha, and extracted forest patch and landscape metrics for each island. The number of primate species per island varied between 0 and 7 species. Primate composition varied substantially according to both island area and forest cover remaining within the landscape, whereas island area alone was the most significant predictor of richness. Locally, tree density and vertical stratification were the most significant explanatory variables of primate composition and richness. A model containing area effects had the most explanatory power regarding site occupancy for most species. Individually, each species responded differently, with howler and brown capuchin monkeys showing greater tolerance to cope with habitat changes. Body size was also an important predictor of primate occupancy. We recommend protecting large fragments and enhancing the suitability of surrounding habitats to ensure primate conservation in most Neotropical fragmented landscapes. Given the flat topography of hydroelectric reservoirs, which mainly favors the formation of small islands, and the escalating hydropower development plans in Amazonia, our findings provide evidence for pervasive detrimental impacts of dams on primate communities. 相似文献
995.
Sándor Dulai István Molnár Dóra Szopkó Éva Darkó András Vojtkó Andrea Sass-Gyarmati Márta Molnár-Láng 《Journal of plant physiology》2014
Osmotic stress responses of water content, photosynthetic parameters and biomass production were investigated in wheat-Aegilops biuncialis amphiploids and in wheat genotypes to clarify whether they can use to improve the drought tolerance of bread wheat. A decrease in the osmotic pressure of the medium resulted in considerable water loss, stomatal closure and a decreased CO2 assimilation rate for the wheat genotypes, while the changes in these parameters were moderate for the amphiploids. Maximal assimilation rate was maintained at high level even under severe osmotic stress in the amphiploids, while it decreased substantially in the wheat genotypes. Nevertheless, the effective quantum yield of PS II was higher and the quantum yield of non-photochemical quenching of PS II and PS I was lower for the amphiploids than for the wheat cultivars. Parallel with this, higher cyclic electron flow was detected in wheat than in the amphiploids. The elevated photosynthetic activity of amphiploids under osmotic stress conditions was manifested in higher biomass production by roots and shoots as compared to wheat genotypes. These results indicate that the drought-tolerant traits of Ae. biuncialis can be manifested in the wheat genetic background and these amphiploids are suitable genetic materials for improving drought tolerance of wheat. 相似文献
996.
Benjamin Rothé Jean-Michel Saliou Marc Quinternet Régis Back Decebal Tiotiu Clémence Jacquemin Christine Loegler Florence Schlotter Vlad Pe?a Kelvin Eckert Solange Moréra Alain Van Dorsselaer Christiane Branlant Séverine Massenet Sarah Sanglier-Cianférani Xavier Manival Bruno Charpentier 《Nucleic acids research》2014,42(16):10731-10747
Biogenesis of eukaryotic box C/D small nucleolar ribonucleoprotein particles (C/D snoRNPs) involves conserved trans-acting factors, which are proposed to facilitate the assembly of the core proteins Snu13p/15.5K, Nop58p/NOP58, Nop56p/NOP56 and Nop1p/Fibrillarin on box C/D small nucleolar RNAs (C/D snoRNAs). In yeast, protein Rsa1 acts as a platform, interacting with both the RNA-binding core protein Snu13 and protein Pih1 of the Hsp82–R2TP chaperone complex. In this work, a proteomic approach coupled with functional and structural studies identifies protein Hit1 as a novel Rsa1p-interacting partner involved in C/D snoRNP assembly. Hit1p contributes to in vivo C/D snoRNA stability and pre-RNA maturation kinetics. It associates with U3 snoRNA precursors and influences its 3′-end processing. Remarkably, Hit1p is required to maintain steady-state levels of Rsa1p. This stabilizing activity is likely to be general across eukaryotic species, as the human protein ZNHIT3(TRIP3) showing sequence homology with Hit1p regulates the abundance of NUFIP1, the Rsa1p functional homolog. The nuclear magnetic resonance solution structure of the Rsa1p317–352–Hit1p70–164 complex reveals a novel mode of protein–protein association explaining the strong stability of the Rsa1p–Hit1p complex. Our biochemical data show that C/D snoRNAs and the core protein Nop58 can interact with the purified Snu13p–Rsa1p–Hit1p heterotrimer. 相似文献
997.
Sabine Filker Marina Kaiser Ramon Rosselló-Móra Micah Dunthorn Gordon Lax Thorsten Stoeck 《Systematic and applied microbiology》2014
The novel ciliate Platynematum salinarum (Scuticociliatia) was isolated only recently from a thalassohaline solar saltern pond (12%) in Portugal. Scanning electron microscopy showed numerous bacterial-shaped cells covering the complete surface of the ciliate. The rod-shaped epibionts were identified and characterized following the “Full-Cycle rRNA Approach”. The almost full-length 16S rRNA gene sequence was obtained using archaeal-specific primers and two species-specific probes were designed for fluorescence in situ hybridization. The 16S rRNA gene sequence of the epibiotic cells showed 87% sequence identity with the type strain sequence of the closest characterized species Halolamina pelagica. Phylogenetic reconstructions affiliated the novel organism to the genus Halolamina (Halobacteria, Archaea). Attempts to isolate the epibionts failed and, therefore, growth experiments incorporating the antibiotic anisomycin were conducted in order to investigate the potential symbiotic relationship between P. salinarum and the epibionts. The results suggested an obligate symbiosis between the two organisms and revealed the first symbiotic representative of the Halobacteria. Based on the phylogenetic analyses and growth experiments we propose the classification of this novel organism in a new genus, with the taxon name “Candidatus Haloectosymbiotes riaformosensis”. 相似文献
998.
Bohumil Sak Klára J. Petr?elková Dana Květoňová Anna Myná?ová Kate?ina Pomajbíková David Modry Michael R. Cranfield Antoine Mudakikwa Martin Kvá? 《PloS one》2014,9(11)
Background
Infectious diseases represent the greatest threats to endangered species, and transmission from humans to wildlife under increased anthropogenic pressure has been always stated as a major risk of habituation.Aims
To evaluate the impact of close contact with humans on the occurrence of potentially zoonotic protists in great apes, one hundred mountain gorillas (Gorilla beringei beringei) from seven groups habituated either for tourism or for research in Volcanoes National Park, Rwanda were screened for the presence of microsporidia, Cryptosporidium spp. and Giardia spp. using molecular diagnostics.Results
The most frequently detected parasites were Enterocytozoon bieneusi found in 18 samples (including genotype EbpA, D, C, gorilla 2 and five novel genotypes gorilla 4–8) and Encephalitozoon cuniculi with genotype II being more prevalent (10 cases) compared to genotype I (1 case). Cryptosporidium muris (2 cases) and C. meleagridis (2 cases) were documented in great apes for the first time. Cryptosporidium sp. infections were identified only in research groups and occurrence of E. cuniculi in research groups was significantly higher in comparison to tourist groups. No difference in prevalence of E. bieneusi was observed between research and tourist groups.Conclusion
Although our data showed the presence and diversity of important opportunistic protists in Volcanoes gorillas, the source and the routes of the circulation remain unknown. Repeated individual sampling, broad sampling of other hosts sharing the habitat with gorillas and quantification of studied protists would be necessary to acquire more complex data. 相似文献999.
?árka Kaňková Lucie Procházková Jaroslav Flegr Pavel Calda Drahomíra Springer Eli?ka Potluková 《PloS one》2014,9(10)
Background
Toxoplasmosis, one of the most common zoonotic diseases worldwide, can induce various hormonal and behavioural alterations in infected hosts, and its most common form, latent toxoplasmosis, influences the course of pregnancy. Autoimmune thyroid diseases (AITD) belong to the well-defined risk factors for adverse pregnancy outcomes. The aim of this study was to investigate whether there is a link between latent toxoplasmosis and maternal AITD in pregnancy.Methods
Cross-sectional study in 1248 consecutive pregnant women in the 9–12th gestational weeks. Serum thyroid-stimulating hormone (TSH), thyroperoxidase antibodies (TPOAb), and free thyroxine (FT4) were assessed by chemiluminescence; the Toxoplasma status was detected by the complement fixation test (CFT) and anti-Toxoplasma IgG enzyme-linked immunosorbent assay (ELISA).Results
Overall, 22.5% of the women were positive for latent toxoplasmosis and 14.7% were screened positive for AITD. Women with latent toxoplasmosis had more often highly elevated TPOAb than the Toxoplasma-negative ones (p = 0.004), and latent toxoplasmosis was associated with decrease in serum TSH levels (p = 0.049). Moreover, we found a positive correlation between FT4 and the index of positivity for anti-Toxoplasma IgG antibodies (p = 0.033), which was even stronger in the TPOAb-positive Toxoplasma-positive women, (p = 0.014), as well as a positive correlation between FT4 and log2 CFT (p = 0.009).Conclusions
Latent toxoplasmosis was associated with a mild increase in thyroid hormone production in pregnancy. The observed Toxoplasma-associated changes in the parameters of AITD are mild and do not seem to be clinically relevant; however, they could provide new clues to the complex pathogenesis of autoimmune thyroid diseases. 相似文献1000.
Márton Doleschall Julianna Anna Szabó Júlia Pázmándi ágnes Szilágyi Klára Koncz Henriette Farkas Miklós Tóth Péter Igaz Edit Gláz Zoltán Prohászka Márta Korbonits Károly Rácz George Füst Attila Patócs 《PloS one》2014,9(9)