青藤碱抑制人宫颈癌的研究

目的：研究青藤碱（sinomenine,SIN）对宫颈癌Hela细胞增殖的影响及其机制,为SIN在宫颈癌的预防和治疗上提供实验依据。方法：不同浓度SIN分别处理体外培养的人宫颈癌细胞系Hela细胞后,采用噻唑蓝（Mar）法检测处理24h、48h、72h后Hela细胞的增殖活性,流式细胞仪测定细胞周期和细胞凋亡。结果：1．0．1、0．2、0．4、0．625、1．25、2．5mmml／L SIN处理Hela细胞24h、48h、72h后,细胞增殖明显受到抑制,呈时间和剂量依赖性特点;2．流式细胞仪细胞周期分析表明,SIN处理组G1期细胞比例明显增加,S期细胞比例明显减少,两组比较有统计学意义;3．细胞凋亡分析表明,SIN处理组细胞凋亡率较对照组升高,呈时间和剂量依赖性特点;结论：SIN在体外能有效抑制宫颈癌细胞生长,其机制可能与其阻滞细胞周期、诱导细胞凋亡有关,SIN有望应用于宫颈癌的辅助治疗。     

Early administration of nifedipine protects against angiotensin II‐induced cardiomyocyte hypertrophy through regulating CaMKII–SERCA2a pathway and apoptosis in rat cardiomyocytes

The calcium channel blocker (CCB), nifedipine, is a more effective treatment for early‐ than late‐stage cardiac hypertrophy. We investigated the effects of early‐ and late‐stage nifedipine administration on calcium homeostasis, CaMKII (Ca²⁺/calmodulin‐dependent protein kinase II) activity and apoptosis of cardiomyocytes under hypertrophic stimulation with angiotensin II (AngII). Primary rat cardiomyocytes were divided into five treatment groups: AK, AngII plus the CaMKII inhibitor, KN‐93; AN‐1 (early‐stage), AngII plus nifedipine × 48 h; AN‐2 (late‐stage), AngII × 48 h, then AngII plus nifedipine × 48 h; C, untreated; and A, AngII × 48 h. The t1/2β [time required for intracellular Ca²⁺ concentration ([Ca²⁺]i) to decline to one half of the peak value] decreased; however, CaMKII and SERCA2a (sarcoplasmic reticulum Ca²⁺‐ATPase 2a) activities increased in the AN‐1 group compared with the AK group. In the AN‐2 group compared with the AN‐1 group, CaMKII activity, t1/2α [time required for [Ca²⁺]i to increase from the bottom to one half of peak value], t1/2β, and apoptosis increased. These results indicate that the timing of CCB administration affects the calcium concentration and apoptosis of hypertrophic cardiomyocytes through the CaMKII–SERCA2a signalling pathway, thereby influencing the drug's protective activity against cardiomyocyte hypertrophy. Copyright © 2016 John Wiley & Sons, Ltd.     

产生葡激酶的溶原性转换噬菌体的研究——Ⅰ.转换噬菌体的分离及宿主双溶原性的检出

从葡激酶阳性菌株66分离到两株噬菌体即α及β,α有转换葡激酶产生的能力,β则无。用去溶原方法由菌株66得到葡激酶阴性66SAK~-,由该菌株诱导得到噬菌体β。证明菌株66为带有两个前噬菌体的双溶原菌。在该菌株经过诱导后,得到的裂解液中,不能转换的噬菌体β多于转换噬菌体α。     

Afatinib Decreases P‐Glycoprotein Expression to Promote Adriamycin Toxicity of A549T Cells

We investigated the reversal effect of afatinib (AFT) on activity of adriamycin (ADR) in A549T cells and clarified the related molecular mechanisms. A549T cells overexpressing P‐glycoprotein (P‐gp) were resistant to anticancer drug ADR. AFT significantly increased the antitumor activity of ADR in A549T cells. AFT increased the intracellular concentration of ADR by inhibiting the function and expression of P‐gp at mRNA and protein levels in A549T cells. Additionally, the reversal effect of AFT on P‐gp mediated multidrug resistance (MDR) might be related to the inhibition of PI3K/Akt pathway. Cotreatment with AFT and ADR could enhance ADR‐induced apoptosis and autophagy in A549T cells. Meanwhile, the co‐treatment significantly induced cell apoptosis and autophagy accompanied by increased expression of cleaved caspase‐3, PARP, LC3B‐II, and beclin 1. Apoptosis inhibitors had no significant effect on cell activity, while autophagy inhibitors decreased cell viability, suggesting that autophagy may be a self protective mechanism of cell survival in the absence of chemotherapy drugs. Interestingly, when combined with AFT and ADR, inhibition of apoptosis and/or autophagy could enhance cell viability. These results indicated that in addition to inhibit P‐gp, ADR‐induced apoptosis, and autophagy promoted by AFT contributed to the antiproliferation effect of combined AFT and ADR on A549T cells. These findings provide evidence that AFT combined ADR may achieve a better therapeutic effect to lung cancer in clinic. J. Cell. Biochem. 119: 414–423, 2018. © 2017 Wiley Periodicals, Inc.     

铜离子对钝顶螺旋藻完整细胞中光系统Ⅱ活性和藻胆体能量传递的影响

Cu2 抑制钝顶螺旋藻 ( Spirulina platensis)完整细胞中电子传递活性 ( H2 O→ MV) ,并抑制 PS 的放氧活性。低浓度的 Cu2 处理 ,使钝顶螺旋藻细胞中藻蓝蛋白荧光发射峰位置蓝移、发射强度改变 ,表明藻胆体中能量传递发生了变化。Cu2 处理纯化的藻蓝蛋白 ,使其在长波区 ( 61 6— 62 0 nm)吸收减小、荧光发射峰蓝移 ( 64 7nm→ 64 3nm) ;而变藻蓝蛋白不受影响 ,说明 Cu2 选择性作用于藻蓝蛋白。     

降水中的PH值和金属元素在松柏混交林内变化动态

 本文以日本滋贺县大津市桐生自然保护区的日本赤松(Pinus densiflora)、黑松(Pinus thunbergii)、扁柏(Chamaecypari sobtusa)混交林小流域为研究地,以若女自然保护区内荒山小流域为对照地。通过对两个小流域为期一年的定位观测研究,测定了林外降水、流出水、穿透水、树干流中的pH值、电导率、钾、钠等14种金属元素以及硅、硼非金属元素。研究结果表明,松柏混交林生态系统和花岗岩发育形成的土壤对雨水的pH值和金属元素等均具有较大的影响,但松柏混交林生态系统对其影响大于荒山。此外,系统内大多数元素的收入大于支出,呈积累趋势。     

冬小麦群体叶片气孔导度差异性分析

对冬小麦 (Triticum aestivum L.)两个品种 3个生育期测定和分析了群体叶片的气孔导度。结果表明 ,近轴面气孔导度比远轴面的大 ,二者之比呈近似正态分布 ,最大概率出现约为 1 .5,该比值在冠层垂直方向变化不显著 ,但随季节而变 ,抽穗期最大。叶片气孔导度从冠层顶部向下迅速递减 ,递减系数约为 0 .57,且各层日变化大致平行。冠层上部气孔活跃 ,导度日变化明显 ,下部日变化不大。同一叶片的气孔导度由近轴面的叶基 ,经由其叶中、叶尖 ,翻过尖点 ,再从远轴面的叶尖经叶中向叶基递减。对近轴面叶中、叶尖气孔导度与该面平均气孔导度相关系数较大 ,而对远轴面叶中、叶基气孔导度与该面气孔导度相关系数较大 ,据此建议测定近轴面气孔导度时 ,应以叶中、叶尖部位为最佳点 ,而测定远轴面气孔导度时 ,应以叶中、叶基部位为最佳点     

满洲里——大杨树一带表土孢粉的散布规律及数量特征

通过现代植被剖面和表土花粉分析 ,阐述了呼伦贝尔高平原 (北部 )到大兴安岭山地 (中段 )一线不同植被类型的孢粉组合特征。在草原带与森林带之间明显地存在森林草原过渡地带 ,其孢粉组合独特 ,木本植物花粉含量略高于草本 ,一般为 30 %～ 70 %。同时 ,还发现花粉含量与母体植物丰度间为非线性关系 ,校正系数 R值在特定状态下才是“常数”。植物 -花粉系统存在三种状态 :优势、混合及劣势状态 ,相应地其 R值各不相同。