全文获取类型
收费全文 | 304篇 |
免费 | 8篇 |
出版年
2021年 | 2篇 |
2019年 | 3篇 |
2018年 | 4篇 |
2016年 | 2篇 |
2015年 | 5篇 |
2014年 | 2篇 |
2013年 | 10篇 |
2012年 | 9篇 |
2011年 | 5篇 |
2010年 | 4篇 |
2009年 | 8篇 |
2008年 | 17篇 |
2007年 | 11篇 |
2006年 | 8篇 |
2005年 | 8篇 |
2004年 | 14篇 |
2003年 | 10篇 |
2002年 | 7篇 |
2001年 | 6篇 |
2000年 | 6篇 |
1999年 | 5篇 |
1998年 | 2篇 |
1995年 | 2篇 |
1994年 | 1篇 |
1992年 | 8篇 |
1991年 | 5篇 |
1990年 | 2篇 |
1989年 | 7篇 |
1988年 | 5篇 |
1987年 | 5篇 |
1986年 | 7篇 |
1985年 | 10篇 |
1984年 | 6篇 |
1983年 | 7篇 |
1982年 | 7篇 |
1979年 | 8篇 |
1978年 | 8篇 |
1977年 | 5篇 |
1976年 | 7篇 |
1975年 | 7篇 |
1974年 | 6篇 |
1973年 | 4篇 |
1972年 | 9篇 |
1971年 | 3篇 |
1970年 | 5篇 |
1969年 | 4篇 |
1968年 | 7篇 |
1967年 | 9篇 |
1966年 | 4篇 |
1965年 | 3篇 |
排序方式: 共有312条查询结果,搜索用时 15 毫秒
81.
82.
Yurika Yogo Seitaro Fujishima Takashi Inoue Fumitake Saito Takayuki Shiomi Kazuhiro Yamaguchi Akitoshi Ishizaka 《Respiratory research》2009,10(1):80
Background
Idiopathic pulmonary fibrosis (IPF) is a chronically progressive interstitial lung disease of unknown etiology. Previously, we have demonstrated the selective upregulation of the macrophage-derived chemokine CCL22 and the thymus activation-regulated chemokine CCL17 among chemokines, in a rat model of radiation pneumonitis/pulmonary fibrosis and preliminarily observed an increase in bronchoalveolar (BAL) fluid CCL22 levels of IPF patients.Methods
We examined the expression of CCR4, a specific receptor for CCL22 and CCL17, in bronchoalveolar lavage (BAL) fluid cells, as well as the levels of CCL22 and CCL17, to elucidate their pathophysiological roles in pulmonary fibrosis. We also studied their immunohistochemical localization.Results
BAL fluid CCL22 and CCL17 levels were significantly higher in patients with IPF than those with collagen vascular diseases and healthy volunteers, and there was a significant correlation between the levels of CCL22 and CCL17 in patients with IPF. CCL22 levels in the BAL fluid did not correlate with the total cell numbers, alveolar lymphocytes, or macrophages in BAL fluid. However, the CCL22 levels significantly correlated with the numbers of CCR4-expressing alveolar macrophages. By immunohistochemical and immunofluorescence analysis, localization of CCL22 and CCR4 to CD68-positive alveolar macrophages as well as that of CCL17 to hyperplastic epithelial cells were shown. Clinically, CCL22 BAL fluid levels inversely correlated with DLco/VA values in IPF patients.Conclusion
We speculated that locally overexpressed CCL22 may induce lung dysfunction through recruitment and activation of CCR4-positive alveolar macrophages. 相似文献83.
Various adhesion molecules impair microvascular leukocyte kinetics in ventilator-induced lung injury
Miyao N Suzuki Y Takeshita K Kudo H Ishii M Hiraoka R Nishio K Tamatani T Sakamoto S Suematsu M Tsumura H Ishizaka A Yamaguchi K 《American journal of physiology. Lung cellular and molecular physiology》2006,290(6):L1059-L1068
Although the endothelial expression of various adhesion molecules substantially differs between pulmonary microvessels, their importance for neutrophil and lymphocyte sequestration in ventilator-induced lung injury (VILI) has not been systematically analyzed. We investigated the kinetics of polymorphonuclear cells (PMN) and mononuclear cells (MN) in the acinar microcirculation of the isolated rat lung with VILI by real-time confocal laser fluorescence microscopy, with or without inhibition of ICAM-1, VCAM-1, or P-selectin by monoclonal antibodies (MAb). Adhesion molecules in each microvessel were estimated by intravital fluorescence microscopy or immunohistochemical staining. In high tidal volume-ventilated lungs, 1) ICAM-1, VCAM-1, and P-selectin were differently upregulated in venules, arterioles, and capillaries; 2) venular PMN rolling was improved by inhibition of ICAM-1, VCAM-1, or P-selectin, whereas arteriolar PMN rolling was improved by ICAM-1 or VCAM-1 inhibition; 3) capillary PMN entrapment was ameliorated only by anti-ICAM-1 MAb; and 4) MN rolling in venules and arterioles and MN entrapment in capillaries were improved by ICAM-1 and VCAM-1 inhibition. In conclusion, the contribution of endothelial adhesion molecules to abnormal leukocyte behavior in VILI-injured microcirculation is microvessel and leukocyte specific. ICAM-1- and VCAM-1-dependent, but P-selectin-independent, arteriolar PMN rolling, which is expected to reflect the initial stage of tissue injury, should be taken as a phenomenon unique to ventilator-associated lung injury. 相似文献
84.
85.
Temporal change in nutrient concentrations and phytoplankton biomass in short time scale local upwelling around the Izu Peninsula, Japan* 总被引:2,自引:0,他引:2
Takahashi M.; Ishizaka J.; Ishimaru T.; Atkinson L.P.; Lee T.N.; Yamaguchi Y.; Fujita Y.; Ichimura S. 《Journal of plankton research》1986,8(6):1039-1049
Temporal changes in nitrate plus nitrite and chlorophyll concentrationswere observed by continuous shipboard measurements in upwelledand coastal water masses covering an area 20 x 30 km2 alongthe Izu Peninsula between May 22 and 26, 1982. Two localizedupwelling events were observed, one which had occurred beforeMay 22 and the other on May 26. Nutrient input into the surfacefrom upwelling and subsequent phytoplankton growth were clearlydocumented. Phytoplankton growth, estimated from the disappearanceof nutrients, agreed well with modelling estimates based uponsimulated culture experiments done previously in the same area.Phytoplankton growth terminated when nutrients were depleted.Similar stimulation of phytoplankton growth supported by localizedupwelling can be expected in other coastal regions where similarconditions exist. 相似文献
86.
Ishizaka N Nakao A Ohishi N Suzuki M Aizawa T Taguchi J Nagai R Shimizu T Ohno M 《FEBS letters》1999,463(1-2):155-159
Leukotriene A(4) (LTA(4)) hydrolase is essential for the conversion of LTA(4) to LTB(4), an inflammatory lipid mediator. We investigated whether LTA(4) hydrolase was regulated in the heart by angiotensin II (ang II) infusion. Continuous ang II infusion via an osmotic minipump for up to 7 days upregulated mRNA and protein levels of LTA(4) hydrolase ( approximately 3.5-fold of control) in the heart in a pressor-dependent manner. Immunohistochemistry demonstrated intense LTA(4) hydrolase staining in the myofibroblast as well as migrated monocytes/macrophages. These data suggest that the cardiac LTA(4) hydrolase-LTB(4) system plays a positive role in the promotion of cardiac inflammation in hypertension. 相似文献
87.
Kajiwara H Kaneko T Ishizaka M Tajima S Kouchi H 《Bioscience, biotechnology, and biochemistry》2003,67(12):2668-2673
Expressed proteins in cultured symbiotic bacteria (Mesorhizobium loti MAFF303099) in the mid-growth phase were proteomically analyzed by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and capillary high-performance liquid chromatography coupled with an ion-trap mass spectrometry (MS). The genome sequence data of M. loti were used to identify the analyzed proteins. We identified 114 of the 127 proteins analyzed on 2D-PAGE gel with some microheterogenities which were caused by post-translational modifications. 相似文献
88.
Growth promotion of mycelia of the Matsutake mushroom Tricholoma matsutake by D-isoleucine 总被引:5,自引:0,他引:5
Kawagishi H Hamajima K Takanami R Nakamura T Sato Y Akiyama Y Sano M Tanaka O 《Bioscience, biotechnology, and biochemistry》2004,68(11):2405-2407
Mycelial growth of the Matsutake mushroom (Tricholama matsutake) was much slower than that of the other mushroom species. We found that the addition of D-isoleucine to the culture medium strikingly promoted mycelia growth. The other amino acids tested had no effect on this growth promotion. 相似文献
89.
Mishima Y Terui Y Mishima Y Katsuyama M Mori M Tomizuka H Takizawa T Miyazato A Ueda M Yamada M Hayasawa H Mizunuma N Ishizaka Y Ikeda K Kato T Ozawa K Hatake K 《Journal of cellular physiology》2002,191(2):183-190
We have established a new hematopoietic cell line from a patient with myelodysplastic syndrome (MDS), which was refractory anemia with excess blasts (RAEB). This cell line, designated TER-3, depends on several cytokines for long-term survival and growth, and requires interleukin-3 (IL-3) for continuous growth. Cytochemical analysis revealed that TER-3 cells are weakly dianisidine positive and nonspecific esterase positive, but peroxidase negative. The surface marker profile shows that the TER-3 cells are strongly positive for myeloid, lymphoid, and megakaryocytic antigens such as CD15, CD19, and CD61, and negative for some common multilineage antigens such as CD13, CD33, and CD34. Thus, this cell line has a multilineage phenotype, suggesting that the transformation event occurred in multipotent stem cells. Dianisidine- and nonspecific esterase-positive TER-3 cells increase with granulocyte-colony stimulating factor (G-CSF) rather than with IL-3. These results suggest that the cell line is useful for understanding the mechanism underlying G-CSF-associated hematopoietic cell differentiation and activation in the patient with MDS. 相似文献
90.
Koji Tsukada Masumi Ishizaka Yukiko Fujisawa Yukimoto Iwasaki Takeshi Yamaguchi Eiichi Minami Naoto Shibuya 《Physiologia plantarum》2002,116(3):373-382
We present evidence that the rice receptor for N -acetylchitooligosaccharide elicitor does not couple to heterotrimeric G-protein (G-protein), one of the most important signal transducers from the cell surface to down-stream effectors in various cellular responses of many organisms. Using mutant rice cells lacking functional G-protein α-subunit, cellular responses of suspension-cultured rice cells derived from Daikoku dwarf ( d1 ) mutants that were shown to contain mutations in the coding region of the G-protein α-subunit (Fujisawa et al. 1999) to N -acetylchitooligosaccharide (oligochitin) elicitor were compared with those of the corresponding parent cell lines. All the elicitor-induced cellular responses, such as medium alkalinization, generation of reactive oxygen species, expression of early responsive genes, PAL and PR genes, and production of phytoalexin, were basically identical in the mutant and wild type cell lines. Considering the reported presence of a single copy gene for the G-protein α-subunit in many plant species including rice, these results strongly support the above conclusion. 相似文献