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61.
内毒素(Endotoxin,简称ET)是百日咳全菌苗(Bordetellapertussis vaccine)产生副作用的主要毒素之一,且不易除去。现有的分离方法,如蔗糖密度梯度离心法,较繁琐,成本高。本文采用Sepha-cryl S-300凝胶层折法可以简便有效的去除大部分内毒素。初步毒素试验结果表明:已达到日本生物制品规格的要求。两种保护性抗原FHA和LPF-HA也得到进一步分离纯化,为今后研制高效的百日咳组分菌苗提供了实验条件。 相似文献
62.
Mu-Chin Tzeng Ming Jhy Hseu Jun Hai Yang Richard John Guillory 《The protein journal》1986,5(3):221-228
Snake presynaptic toxins such as crotoxin, β-bungarotoxin and taipoxin block neuromuscular transmission through inhibiting the release of acetylcholine by their phospholipase A2 activities. On the other hand, many other phospholipase A2s show little neurotoxicity. It is likely that the difference lies in whether high affinity binding to nerve cell membranes exists or not. To test this idea, crotoxin, β-bungarotoxin and taipoxin were first radioactively labeled with Na(125I) without loss of their neurotoxicity. Using the radioactive toxins we have found that each of the three showed specific binding to synaptosomal membranes from guinea pig brain. In contrast, we could not detect specific binding of a non-neurotoxic pancreatic phospholipase A2. Crotoxin and taipoxin, but not β-bungarotoxin, also bound specifically to membrane preparation from other tissues. The binding of each toxin was not greatly affected by the other two toxins. The photoaffinity labeling technique has been used to obtain further information about the components which bind crotoxin. For this purpose, (125I) crotoxin was derivatized with N-hydroxysuccinimidyl-4-azidobenzoate. Autoradiographic analysis of the membranes following photoirradiation in the presence of the modified crotoxin revealed that an 85K dalton component was preferentially covalently conjugated with the crotoxin analogue in a specific manner. 相似文献
63.
Dual action of respiratory inhibitors: inhibition of germination and prevention of dormancy induction in lettuce seeds 下载免费PDF全文
`Grand Rapids' lettuce Lactuca sativa L. seeds germinate readily at 15°C but poorly at 25°C in darkness. When held in dark at 25°C for an extended period, the ungerminated seeds become dormant as shown by their inability to germinate or transfer to 15°C in darkness. Induction of dormancy at 25°C was prevented by exposure to CN−, azide, salicylhydroxamic acid (SHAM), dinitrophenol, and pure N2 as determined by subsequent germination at 15°C on removal of inhibitors. The effectiveness of inhibitors to break dormancy declined as dormancy intensified. At relatively low levels, CN−, SHAM, and azide promoted dark germination at 25°C while at high levels they were inhibitory. Uptake of O2 by seeds held at 25°C for 4 days in 1.0 millimolar KCN was inhibited by 67% but was promoted 61% when KCN was removed. Correspondingly greater inhibition (79%) and promotion (148%) occurred when 1.0 millimolar SHAM was added to KCN solution. When applied alone, SHAM had little effect on O2 uptake. These data indicate that Cyt pathway of respiration plays a dominant role in the control of both dormancy induction and germination of lettuce seeds, and `alternative pathway' is effectively engaged in presence of CN−. The channeling of respiratory energy use for processes governing germination or dormancy is subject to control by physical and chemical factors.
A scheme is proposed that illustrates compensatory use of energy for processes controlling dormancy induction and germination. A block of germination, e.g. by low water potential polyethylene glycol solution or a supraoptimal temperature spares energy to be utilized for dormancy induction while a block of dormancy induction by low levels of CN− (similar to GA and light effects) drives germination. Blocking both processes by inhibitors (e.g. CN−, CN− + SHAM) presumably leads to accumulation of `reducing power' with consequent improvement in O2 uptake and oxidation rates of processes controlling germination or dormancy induction upon removal of the inhibitors.
相似文献64.
Individual 14C-labelled amino acids are rapidly removed from dilute solution in artificial sea water (0.2 mol 1–1) by suspensions of Meliosira medocris. The rate of disappearance of radioactivity corresponds closely to removal of primary amines as determined by measurement of the rate of decrease of fluorescamine-positive material. Net removal of naturally occurring free amino acids from the sea water habitat from which the alga was isolated is demonstrated using high performance liquid chromatography. Removal of amino acids from natural sources makes a significant contribution to the carbon requirements of the alga as well as supplying significant amounts of amino nitrogen. 相似文献
65.
66.
明适应条件下鲤属鱼L-型外水平细胞反应的给光-瞬变成分 总被引:1,自引:1,他引:0
在明适应条件下鲤属鱼 L-型外水平细胞的反应显示明显的给光-瞬变成分(on-transient)、它与刺激波长有关——对蓝、绿光的反应比对红光的反应有更明显的瞬变成分,其光谱特性提示它与绿敏锥细胞的输入信号有关。与已报道的其它动物 L 型水平细胞的给光-瞬变成分不同,它的出现在一定范围内与网膜受照射的面积无关。绿色(502nm)和红色(706nm)闪光同时照射所引起反应的给光-瞬变成分比各自单独刺激时要显著得多,提示它也与绿敏锥细胞和红敏锥细胞输入的相互作用有关。 相似文献
67.
白纹伊蚊实验种群动态的研究 总被引:4,自引:0,他引:4
在实验室条件下观察白纹伊蚊种群发育、存活和繁殖的动态,得出年龄特征存活率、繁殖率等数据,编制成生命表,计算出有关种群各项参数,包括内禀增长能力(rm=0.1333),净增殖率(R0=45.3017),增殖有限速率(λ=1.1426),平均世代周期长(T=33.3299天),瞬时出生率和死亡率(b=0.5562,d=0.4229),稳定年龄组配,成虫前期占90.66%,成虫期占9.34%。该蚊种群大约每隔5.2天增加一倍。根据结果对该蚊种群传播登革热的关系和防制加以讨论。 相似文献
68.
69.
番茄五个品种的核型研究(摘要) 总被引:1,自引:0,他引:1
本文研究了番茄五个不同品种(武昌大红、加拿大、佛特、荷兰五号、青北早)的核型。1.番茄的核型公式如下: (1) 武昌大红。2n=24=2M+8m+12sm+2st (2) 加拿大。2n=24=4M+8m+12sm (3) 佛特。2n=24=2M+8m+10sm+4st (4) 荷兰五号2n=24=2M+14m+8sm (5) 青北早2n=24=4M+8m+12sm 2、五个品种中都具有一对随体染色体,但位于那一对染色体上也有相同和相异。“武昌大红”与“佛特”的随体在第9对上。“加拿大”在第7对上。“荷兰五号”及“青北早”位于第4对染色体上,并发现“武昌大红”、“加拿大”品种的随体,在同一品种也出现随体大小的差异。3、在各个品种中,染色体的全长、相对长度、臂比都存在着一些差异。4、对于番茄染色体的随体的大小及其在染色体对数的位置,染色体的形态结构,核形与番茄起源的关系进行了初步讨论。 相似文献
70.