Luteolin Protects Against CIRI,Potentially via Regulation of the SIRT3/AMPK/mTOR Signaling Pathway

Neurochemical Research - Mitochondrial abnormalities accelerate the progression of ischemic brain damage. Sirtuin 3 (SIRT3) is mainly found in mitochondria and affects almost all major aspects of...     

基于不同利益相关者的水电能源基地建设经济损益研究——以澜沧江干流为例

水电开发将在局地、区域以及全球尺度上产生一系列的影响,明确这些尺度上利益相关者的成本效益、合理分配水电开发利益是减少水电开发不利影响、促进水电资源可持续利用的关键。以澜沧江干流水电能源基地建设为背景,从开发企业、移民农户、澜沧江流域政府及全球利益相关者4个利益相关者角度,选取经济、生态环境和移民生计指标对水电开发的利益再分配进行评估,结果显示:尽管水电开发总体效益大于成本,比例为4.27∶1,且水电开发所涉及的开发企业、政府流域及全球利益相关者所获得的利益大于成本,成本效益比例分别为1∶3.93、1∶3.10、1∶13.11;但移民农户成本却大于效益,比例为1.48∶1。水电开发企业等利益主体应增加生态补偿强度,才能确保移民农户的净收益不降低。该研究表明:不同利益相关者分析有助于了解成本效益分配,清晰展示利益流向,减少开发项目中的不利影响,结果可为协调水电能源开发利用与流域可持续发展提供科学依据。     

Indoline and piperazine containing derivatives as a novel class of mixed D(2)/D(4) receptor antagonists. Part 1: identification and structure-activity relationships

Optimization of the lead compound 2-[-4-(4-chloro-benzyl)-piperazin-1-yl]-1-(2,3-dihydro-indol-1-yl)-ethanone 1 by systematic structure-activity relation (SAR) studies lead to two potent compounds 2-[-4-(4-chloro-benzyl)-piperazin-1-yl]-1-(2-methy-2,3-dihydro-indol-1-yl)-ethanone 2n and 2-[-4-(4-chloro-benzyl)-piperazin-1-yl]-1-(2-methy-2,3-dihydro-indol-1-yl)-ethanone 7b. Their related synthesis was also reported.     

药物对基于SERS技术的尿液中PDGF-BB检测影响研究

本文旨在研究基于表面增强拉曼光谱(SERS)技术检测尿液中血小板衍生生长因子(PDGF-BB)从而对冠状动脉堵塞程度进行快速无损诊断的方法是否同样适用于长期服用药物(阿司匹林、瑞舒伐他汀或硫酸氢氯吡格雷)的冠心病患者或行经皮冠状动脉介入手术(PCI术)后病人的冠状动脉堵塞程度检测。首先基于该方法对13例长期服用药物(阿司匹林、瑞舒伐他汀或硫酸氢氯吡格雷)的冠心病患者和13例行PCI术后病人的尿液样本进行检测,发现其SERS光谱中均无PDGF-BB位于1 509 cm^-1的拉曼特征峰。然后进一步分析PDGFBB水溶液与三种药物的SERS光谱,也均未发现1 509 cm^-1的拉曼特征峰。结果表明,这三种药物均对PDGFBB的SERS信号产生了影响,可见该方法并不适用于长期服用药物的冠心病患者或行PCI病人术后的冠状动脉堵塞程度的检测。     

Design,characterization, and in vitro antiproliferative efficacy of gemcitabine conjugates based on carboxymethyl glucan

Gemcitabine (GEM) is widely used in clinical practice in the treatment of cancer and several other solid tumors. Nevertheless, the antitumor effect of GEM is partially prevented by some limitations including short half life, and lack of tumor localizing. Carboxymethyl glucan (CMG), a carboxymethylated derivative of β-(1-3)-glucan, shows biocompatibility and biodegradability as well as a potential anticarcinogenic effect. To enhance the antiproliferative activity of GEM, four water soluble conjugates of GEM bound to CMG via diverse amino acid linkers were designed and synthesized. ¹H NMR, FT IR, elementary analysis and RP-HPLC chromatography were employed to verify the correct achievement of the conjugates. In vitro release study indicated that conjugates presented slower release in physiological buffer (pH 7.4) than acidic buffer (pH 5.5) mimicking the acidic tumor microenvironment. Moreover, A549, HeLa and Caco-2 cancer cell lines were used to evaluate the in vitro cytotoxicity of conjugates and the results showed that binding GEM to CMG significantly enhanced antiproliferative activity of GEM on A549 cells. Therefore, these conjugates may be potentially useful as a delivery vehicle in cancer therapy and worthy of further study on structure-activity relationship and antiproliferative activity in vitro and in vivo, especially for lung tumor.     

柳蝙蛾生物学特性及发生规律

于１９９７～２０００年在黑龙江和吉林东部地区,对柳蝙蛾的生物学习性和发生规律进行了研究。在该地区柳蝙蛾多数个体２年完成一个世代,分别以卵和幼虫２次越冬。每年６月底、７月上旬,幼虫转移到水曲柳上危害。上树后的幼虫,在室内人工饲养条件下发育了６龄仍未化蛹。在水曲柳人工林中,主要危害２～２０年的水曲柳人工幼林的干基部（２０ｃｍ以下）和干部。水曲柳蝙蝠蛾的发生量与林龄和林分结构密切相关。以水曲柳人工纯林以     

Study on the relationship between Helicobacter pylori in the dental plaque and the occurrence of dental caries or oral hygiene index

Background: The aims of our study were to determine the presence of Helicobacter pylori DNA in the dental plaque of Chinese children aged 3–6 years by nested polymerase chain reaction (PCR) and to investigate the relationship between this infection and the occurrence of dental caries or oral hygiene index.
Methods: Two hundred and fourteen children from a kindergarten in Guangzhou City of China were evaluated. The children's plaques were assessed by plaque indices of Quigley–Hein. Dental plaque was analyzed using nested PCR for two sets of primers directed to the 860-bp fragment of H. pylori genomic DNA, which have been reported to be highly sensitive and specific by other researchers.
Results: H. pylori was detected in dental plaque samples from 126 children, and 70 children with dental caries carried H. pylori in dental plaque. Of these children without infection, only 36 of 88 suffered dental caries. Besides, the average dental plaque index of 126 H. pylori -positive children was higher than that of 88 children without infection. In the present study, there was a significant correlation between H. pylori infection and dental caries or dental hygiene.
Conclusion: The oral cavity may be a reservoir for H. pylori infection in children. H. pylori in dental plaque may play a role in the occurrence of dental caries, and poor oral hygiene may represent a risk factor for H. pylori in the oral cavity.     

Overexpression of a poplar two-pore K+ channel enhances salinity tolerance in tobacco cells

Populus euphratica is a plant model intensively studied for elucidating physiological and molecular mechanisms of salt tolerance in woody species. Several studies have shown that vacuolar potassium (K⁺) ion channels of the two-pore K⁺ (TPK) family play an important role in maintaining K⁺ homeostasis. Here, we cloned a putative TPK channel gene from P. euphratica, termed PeTPK. Sequence analysis of PeTPK1 identified the universal K-channel-specific pore signature, TXGYGD. Over-expression of PeTPK1 in tobacco BY-2 cells improved salt tolerance, but did not enhance tolerance to hyperosmotic stress caused by mannitol (200?C600?mM). After 3?weeks of NaCl stress (100 and 150?mM), PeTPK1-transgenic cells had higher fresh and dry weights than wild-type cells. Salt treatment caused significantly higher Na⁺ accumulation and K⁺ loss in wild-type cells compared to transgenic cells. During short-term salt stress (100?mM NaCl, 24-h), PeTPK1-transgenic cells showed higher cell viability and reduced membrane permeabilization compared to wild-type cells. Scanning ion-selective electrode data revealed that salt-shock elicited a significantly higher transient K⁺ efflux from PeTPK1-transgenic callus cells and protoplasts compared to that observed in wild-type cells and protoplasts. We concluded that salt tolerance in P. euphratica is most likely mediated through PeTPK1. We propose that, under salt stress, PeTPK1 functions as an outward-rectifying, K⁺ efflux channel in the vacuole that transfers K⁺ to the cytosol to maintain K⁺ homeostasis.     

Life cycle and morphology of Physarum pusillum (Myxomycetes) on agar culture

Myxomycetes (slime molds) are unique eukaryotic microorganisms with both characteristics of fungi and amoebae. Artificial cultures grown under controlled conditions were used to study the life cycle and morphogenesis. Physarum pusillum was collected from the field. Spores were inoculated and cultured with the hanging drop method. The complete life cycle was observed from spore to spore on agar without adding any solid nutrients or bacteria as food. Life cycle morphological characteristics were described for spore germination, myxamoebae, zygote, plasmodium and sporangia formation.     

Gender Differences in the Risk Factors for Endothelial Dysfunction in Chinese Hypertensive Patients: Homocysteine Is an Independent Risk Factor in Females

ObjectivesEndothelial dysfunction plays a key role in the pathogenesis of cardiovascular disease. However, the gender-related differences in risk factors for endothelial dysfunction are controversial. We investigated the gender differences in the risk factor profiles for endothelial dysfunction in Chinese hypertensive patients.MethodsVascular endothelial functions in 213 hypertensive patients were measured by digital reactive hyperemia peripheral arterial tonometry (RH-PAT). Peripheral blood samples were collected, and the self-reported smoking and alcohol consumption status, age, body mass index, heart rate, blood pressure and drug administrations were recorded.ResultsRH-PAT indexes were attenuated in both male and female hypertensive patients [1.60 (1.38-2.02) vs. 1.63 (1.44-1.98)]. Multivariate logistic regression analysis identified plasma creatinine (p < 0.001), total cholesterol (p = 0.001), homocysteine (p = 0.002) and smoking (p < 0.001) as the independent factors correlated with gender (male). Multivariate linear regression analysis further identified homocysteine as the factor that is significantly and independently correlated with the decrease in the RH-PAT indexes in female patients (odds ratio: -0.166, 95% confidence interval: -0.292 to -0.040, p = 0.01). However, none of these four factors were correlated with the RH-PAT indexes in male patients.ConclusionsThere are gender-related differences in the risk factors for endothelial dysfunction in Chinese hypertensive patients. Homocysteine is an independent factor for endothelial dysfunction in female hypertensive patients.