全文获取类型
收费全文 | 1674篇 |
免费 | 128篇 |
国内免费 | 74篇 |
出版年
2023年 | 8篇 |
2022年 | 24篇 |
2021年 | 71篇 |
2020年 | 40篇 |
2019年 | 59篇 |
2018年 | 55篇 |
2017年 | 42篇 |
2016年 | 73篇 |
2015年 | 111篇 |
2014年 | 121篇 |
2013年 | 128篇 |
2012年 | 160篇 |
2011年 | 127篇 |
2010年 | 85篇 |
2009年 | 71篇 |
2008年 | 70篇 |
2007年 | 86篇 |
2006年 | 68篇 |
2005年 | 75篇 |
2004年 | 57篇 |
2003年 | 58篇 |
2002年 | 48篇 |
2001年 | 38篇 |
2000年 | 20篇 |
1999年 | 32篇 |
1998年 | 17篇 |
1997年 | 7篇 |
1996年 | 11篇 |
1995年 | 13篇 |
1994年 | 15篇 |
1993年 | 9篇 |
1992年 | 10篇 |
1991年 | 13篇 |
1990年 | 6篇 |
1989年 | 9篇 |
1988年 | 4篇 |
1987年 | 4篇 |
1986年 | 9篇 |
1985年 | 2篇 |
1984年 | 4篇 |
1983年 | 4篇 |
1980年 | 1篇 |
1976年 | 3篇 |
1975年 | 2篇 |
1974年 | 1篇 |
1973年 | 1篇 |
1972年 | 2篇 |
1970年 | 2篇 |
排序方式: 共有1876条查询结果,搜索用时 203 毫秒
91.
Stem cell aging is controlled both intrinsically and extrinsically in the Drosophila ovary 总被引:2,自引:0,他引:2
It is widely postulated that tissue aging could be, at least partially, caused by reduction of stem cell number, activity, or both. However, the mechanisms of controlling stem cell aging remain largely a mystery. Here, we use Drosophila ovarian germline stem cells (GSCs) as a model to demonstrate that age-dependent decline in the functions of stem cells and their niche contributes to overall stem cell aging. BMP signaling activity from the niche significantly decreases with age, and increasing BMP signaling can prolong GSC life span and promote their proliferation. In addition, the age-dependent E-cadherin decline in the stem cell-niche junction also contributes to stem cell aging. Finally, overexpression of SOD, an enzyme that helps eliminate free oxygen species, in either GSCs or their niche alone can prolong GSC life span and increase GSC proliferation. Therefore, this study demonstrates that stem cell aging is controlled extrinsically and intrinsically in the Drosophila ovary. 相似文献
92.
探究了新疆盐生植物黑果枸杞不同组织中内生真菌群落的组成及多样性,为深入研究盐碱、干旱等环境下内生真菌与宿主的互作机制,筛选和开发促生、防病和抗逆等功能的有益内生真菌资源提供科学依据。利用ITS高通量测序技术比较黑果枸杞不同组织真菌群落组成及差异,并采用FUNGuild数据库预测真菌群落生态功能。测序共获得高质量内生真菌序列354 058条,涉及291个OTUs,分属于9门、19纲、34目、50科、60属。α多样性指数分析表明,根、果中真菌群落多样性和丰富度程度较高,叶居中,花的多样性最低,茎的丰富度最低。对黑果枸杞内生真菌群落组成和优势菌群的分析表明,子囊菌门为优势菌门,花、叶、果、茎和根中相对丰度分别为86.85%、72.36%、75.97%、84.44%和85.02%。链格孢属为黑果枸杞植株中的核心属,不同组织中均有分布,在花、叶、果、茎和根中占比分别为85.41%、69.79%、47.07%、79.94%和36.97%。较优势的菌尚有枝孢属、枝顶孢属、新凸轮孢菌属、茎点霉属和楔孢黑粉菌属等真菌。这些菌多具有促生、抗逆等特性,在宿主对盐碱、干旱等极端环境的适应性方面有着潜在功能。各组织独有属的相对丰度均未超过1%。共有菌和独有菌在不同组织中的组成和相对丰度差异较大。经FUNGuild软件平台解析显示,黑果枸杞中病理-腐生-共生营养型的相对丰度最高,在花、叶、果、茎和根中分别为85.41%、69.84%、47.24%、79.98%和37.09%。果和叶中含多种相对丰度≥1%的条件致病菌,而根中腐生真菌种类较多。黑果枸杞中蕴含着功能丰富的内生真菌菌群,在不同组织中的组成及其生态功能差异较大,同时还含有大量未鉴定出种属和未定义的功能菌群,这些可为黑果枸杞功能菌群发掘提供数据参考。 相似文献
93.
水韭属(Iso235;tes)是起源最为古老的水生维管植物,全属物种均被列为国家一级重点保护植物。通过对全国水韭属植物的调查和研究发现,不同产地的四倍体植株在形态上存在显著差异。基于形态学、孢粉学和细胞学证据,将分布于中国湖南省长沙地区和怀化地区的四倍体居群分别命名为隆平水韭(Iso235;tes longpingii)和湘妃水韭(I. xiangfei),并详细描述了其形态特征。隆平水韭形态上与中华水韭(I. sinensis)相似,不同之处在于其大孢子具小的瘤状或冠状纹饰,叶细长而柔弱,长达60 cm; 该种也与六倍体东方水韭(I. orientalis)相似,不同之处在于其染色体44条,大孢子具瘤状或冠状纹饰。湘妃水韭的大孢子纹饰虽与二倍体云贵水韭(I. yunguiensis)相似,但在小孢子纹饰、孢子囊形状和染色体数目方面却不同。隆平水韭仅少数植株生长于湖南省宁乡市一处池塘,完全沉水生长,而湘妃水韭则分布于怀化市通道县和会同县的湿地。由于这两个新种的分布区狭窄,野生居群数量和个体数较少,栖息地环境受到人为干扰,因此根据IUCN红色名录评估标准,将隆平水韭评为极危(CR)等级,湘妃水韭评为易危(VU)等级。所编制的中国已知水韭属物种的分种检索表,为本属物种的鉴定和保护工作提供了重要参考。 相似文献
94.
Guan Wang Zong-Jian Liu Xuan Liu Feng-Ge Liu Yan Li Yi-Bing Weng Jian-Xin Zhou 《Journal of cellular physiology》2019,234(11):20118-20127
This study aims to determine the feasibility of using oligodeoxynucleotides with unmethylated cytosine-guanine dinucleotide sequences (CpG ODN) as an immunity protection strategy for a mouse model of acute respiratory distress syndrome (ARDS). This is a prospective laboratory animal investigation. Twenty-week-old BALB/c mice in Animal research laboratory were randomized into groups. An ARDS model was induced in mice using lipopolysaccharides (LPSs). CpG ODN was intranasally and transrectally immunized before or after the 3rd and 7th days of establishing the ARDS model. Mice were euthanized on Day 7 after the second immunization. Then, retroorbital bleeding was carried out and the chest was rapidly opened to collect the trachea and tissues from both lungs for testing. CpG ODN significantly improved the pathologic impairment in mice lung, especially after the intranasal administration of 50 μg. This resulted in the least severe lung tissue injury. Furthermore, interleukin-6 (IL-6) and IL-8 concentrations were lower, which was second to mice treated with the rectal administration of 20 µg CpG ODN. In contrast, the nasal and rectal administration of CpG ODN in BALB/c mice before LPS immunization did not appear to exhibit any significant protective effects. The intranasal administration of CpG ODN may be a potential treatment approach to ARDS. More studies are needed to further determine the protective mechanism of CpG ODN. 相似文献
95.
Claassens Anders Rose Michael T. Van Zwieten Lukas Weng Zhe Rose Terry J. 《Plant and Soil》2019,439(1-2):393-404
Plant and Soil - Some studies have shown that an increasing atmospheric CO2 concentration reduces plant transpiration while others have demonstrated that it interacts with nutrients in soil to... 相似文献
96.
97.
Journal of Insect Behavior - Honey bees have three castes, drones, workers, and queens, that accomplish different tasks. In this research, we revealed the divergence in feeding behavior of drones... 相似文献
98.
99.
100.
The crystal structure of praseodymium chloride.alpha-D-ribopyranose pentahydrate, PrCl3-C5H10O5-5 H2O, M(r)=487.47, a=9.1989(8), b=8.8214(7), c=9.8233(9) A, beta=94.060(3) degrees, V=795.2(1) A(3), Z=2, mu=0.71073 A and R=0.0418 for 1923 observed reflections and 172 parameters has been determined. The sugar provides three hydroxyl groups, ax-eq-ax for coordination. The Pr(3+) ion is nine-coordinated with five Pr-O bonds from water molecules, three from hydroxyl groups and one from chloride. The OH, CO stretching vibrations and COH bending vibrations are shifted in the complex IR spectrum and the hydroxyl groups, water molecules, chloride ions form an extensive hydrogen-bond network. 相似文献