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51.
K. Hamasu K. Shigemi Y. Tsuneyoshi H. Yamane H. Sato D. M. Denbow M. Furuse 《Amino acids》2010,38(1):57-64
The central effects of L-proline, D-proline and trans-4-hydroxy-L-proline were investigated by using the acute stressful model with neonatal chicks in Experiment 1. Sedative and hypnotic
effects were induced by all compounds, while plasma corticosterone release under isolation stress was only attenuated by L-proline.
To clarify the mechanism by which L-proline and D-proline induce sedative and hypnotic effects, the contribution of the strychnine-sensitive
glycine receptor (glycine receptor) and N-methyl-D-aspartate glutamate receptor (NMDA receptor) were further investigated. In Experiments 2–3, the glycine receptor
antagonist strychnine was co-injected intracerebroventricular (i.c.v.) with L-proline or D-proline. The suppression of isolation-induced
stress behavior by D-proline was attenuated by strychnine. However, the suppression of stress behavior by L-proline was not
attenuated. In Experiment 4, the NMDA receptor antagonist (+)-MK-801 was co-injected i.c.v. with L-proline. The suppression
of stress behavior by L-proline was attenuated by (+)-MK-801. These results indicate that L-proline and D-proline differentially
induce sedative and hypnotic effects through NMDA and glycine receptors, respectively. 相似文献
52.
Bax-inhibiting peptide derived from mouse and rat Ku70 总被引:5,自引:0,他引:5
Yoshida T Tomioka I Nagahara T Holyst T Sawada M Hayes P Gama V Okuno M Chen Y Abe Y Kanouchi T Sasada H Wang D Yokota T Sato E Matsuyama S 《Biochemical and biophysical research communications》2004,321(4):961-966
Bax is a proapoptotic protein that plays a key role in the induction of apoptosis. Ku70 has activities to repair DNA damage in the nucleus and to suppress apoptosis by inhibiting Bax in the cytosol. We previously designed peptides based on the amino acid sequence of Bax-binding domain of human Ku70, and showed that these peptides bind Bax and inhibit cell death in human cell lines. In the present report, we examined the biological activities of other pentapeptides, VPTLK and VPALR, derived from mouse and rat Ku70. Cells in culture accumulated FITC-labeled VPTLK and VPALR, indicating that these peptides are cell permeable (human, mouse, rat, and porcine cells were examined). These peptides bound to Bax and suppressed cell death in various cell types including primary cultured cells. These data suggest that such Bax inhibiting peptides from three mammalian species may be used to protect healthy cells from apoptotic injury under pathological conditions. 相似文献
53.
Phosphodiesterase type 4 inhibitor reduces the retention of polymorphonuclear leukocytes in the lung
Sato Y Sato S Yamamoto T Ishikawa S Onizuka M Sakakibara Y 《American journal of physiology. Lung cellular and molecular physiology》2002,282(6):L1376-L1381
Phosphodiesterase (PDE) type 4 is the predominant PDE isozyme in polymorphonuclear leukocytes (PMN) and plays a key role in the regulation of PMN activation. The aim of this study was to examine the effect of a PDE type 4 inhibitor, rolipram, on the functional changes and the retention of PMN in the lung. In vitro, F-actin content and L-selectin and CD11b expression of PMN stimulated by N-formyl-Met-Leu-Phe were measured by flow cytometry. PMN deformability was evaluated using silicon microchannels. Rolipram reduced the increase of F-actin and CD11b but did not change the decrease of L-selectin. Rolipram inhibited the increase of the transit time of PMN through the microchannel. We evaluated the retention of PMN in the lung in vivo by infusing labeled blood into the vena cava and examining the recovery into aortic root samples in rabbits. Rolipram inhibited the retention of stimulated PMN in the lung. In conclusion, a PDE type 4 inhibitor, rolipram, reduces the retention of PMN in the lung by reducing deformability change and CD11b upregulation of PMN. 相似文献
54.
Tie J Suzuki Y Tsukamoto S Oshida S 《Human biology; an international record of research》2002,74(4):601-606
Allele frequencies and sequence characteristics of the D2S1242 short tandem repeat (STR) locus were studied in a Japanese population sample. A total of 10 D2S1242 alleles and 34 genotypes were identified in 273 unrelated Japanese individuals. The five most common alleles detected had frequencies of over 10%. No deviations from Hardy-Weinberg equilibrium were found when the expected allele values were compared with the observed values. Sequence analysis of each allele showed a tetranucleotide polymorphism. Alleles 9 to 14 had different sequence structures than alleles 15 to 19. Allele 18 had a different sequence in the Japanese sample compared to an Austrian sample. The power of discrimination was 0.95. The present results demonstrate that the D2S1242 STR locus is a useful genetic marker in the Japanese population. 相似文献
55.
Primary structural features of rosaceous S-RNases associated with gametophytic self-incompatibility 总被引:18,自引:0,他引:18
Ishimizu Takeshi Shinkawa Toyohide Sakiyama Fumio Norioka Shigemi 《Plant molecular biology》1998,37(6):931-941
We isolated cDNA clones encoding five S-RNases (S1-,S3- , S5-, S6-, S7-RNases) from pistils of Pyrus pyrifolia (Japanese pear), a member of the Rosaceae. Their amino acid sequences were aligned with those of other rosaceous S-RNases sequenced so far. A total of 76 conserved amino acid residues were stretched throughout the sequence, but were absent from the 51–66 region which was designated the hypervariable (HV) region. The phylogenetic tree of rosaceous S-RNases showed that S-RNase polymorphism predated the divergence of Pyrus and Malus. Pairwise comparison of these S-RNases detected two highly homologous pairs, P. pyrifolia S1- and S4-RNases (90.0%) and P. pyrifolia S3- and S5-RNases (95.5%). The positions of amino acid substitutions between S1- and S4-RNases were spread over the entire region, but in the pair of S3- and S5-RNases, amino acid substitutions were found in the 21–90 region including the HV region. The substitutions in this restricted region appear to be sufficient to discriminate between S3 and S5 pollen and to trigger the self-incompatible reaction. 相似文献
56.
Yasukazu Nakamura Takakazu Kaneko Shusei Sato Mamoru Mimuro Hideaki Miyashita Tohru Tsuchiya Shigemi Sasamoto Akiko Watanabe Kumiko Kawashima Yoshie Kishida Chiaki Kiyokawa Mitsuyo Kohara Midori Matsumoto Ai Matsuno Naomi Nakazaki Sayaka Shimpo Chie Takeuchi Manabu Yamada Satoshi Tabata 《DNA research》2003,10(4):181-201
57.
Kei'ichi Baba ;Yong Woo Park ;Tomomi Kaku ;Rumi Kaida ;Miyuki Takeuchi ;Masato Yoshida ;Yoshihiro Hosoo ;Yasuhisa Ojio ;Takashi Okuyama ;Toru Taniguchi ;Yasunori Ohmiya ;Teiji Kondo ;Ziv Shani ;Oded Shoseyov ;Tatsuya Awano ;Satoshi Serada ;Naoko Norioka ;Shigemi Norioka ;Takahisa Hayashi 《植物生理学报》2009,(5):893-903
In response to environmental variation, angiosperm trees bend their stems by forming tension wood, which consists of a cellulose-rich G (gelatinous)-Iayer in the walls of fiber cells and generates abnormal tensile stress in the secondary xylem. We produced transgenic poplar plants overexpressing several endoglycanases to reduce each specific polysaccharide in the cell wall, as the secondary xylem consists of primary and secondary wall layers. When placed horizontally, the basal regions of stems of transgenic poplars overexpressing xyloglucanase alone could not bend upward due to low strain in the tension side of the xylem. In the wild-type plants, xyloglucan was found in the inner surface of G-layers during multiple layering. In situ xyloglucan endotransglucosylase (XET) activity showed that the incorporation of whole xyloglucan, potentially for wall tightening, began at the inner surface layers S1 and S2 and was retained throughout G-layer development, while the incorporation of xyloglucan heptasaccharide (XXXG) for wall loosening occurred in the primary wall of the expanding zone. We propose that the xyloglucan network is reinforced by XET to form a further connection between wall-bound and secreted xyloglucans in order to withstand the tensile stress created within the cellulose G-layer micro fibrils. 相似文献
58.
Shirasawa-Seo N Sano Y Nakamura S Murakami T Gotoh Y Naito Y Hsia CN Seo S Mitsuhara I Kosugi S Ohashi Y 《Plant cell reports》2005,24(3):155-163
The activity of a predicted promoter, PMC8, from Milk vetch dwarf virus was evaluated by comparing it with the cauliflower mosaic virus 35S RNA promoter (P35S) and PNCR, a promoter from Soybean chlorotic mottle virus. When the GUS fusion gene was introduced into tobacco, PMC8 showed a similar expression profile to P35S but with a more intense expression in proliferating tissues. The usefulness of PMC8 was confirmed by driving NPTII for selection of kanamycin-resistant tobacco plants with improved transformation efficiency. PMC8 was also effective in transgenic rice plants. Thus, PMC8 is useful as an alternative to P35S in both dicotyledonous and monocotyledonous plants, especially for gene expression in proliferating tissues. 相似文献
59.
The vertebrate urogenital system forms due to inductive interactions between the Wolffian duct, its derivative the ureteric bud, and their adjacent mesenchymes. These establish epithelial primordia within the mesonephric (embryonic) and metanephric (adult) kidneys and the Müllerian duct, the anlage of much of the female reproductive tract. We show that Wnt9b is expressed in the inductive epithelia and is essential for the development of mesonephric and metanephric tubules and caudal extension of the Müllerian duct. Wnt9b is required for the earliest inductive response in metanephric mesenchyme. Further, Wnt9b-expressing cells can functionally substitute for the ureteric bud in these interactions. Wnt9b acts upstream of another Wnt, Wnt4, in this process, and our data implicate canonical Wnt signaling as one of the major pathways in the organization of the mammalian urogenital system. Together these findings suggest that Wnt9b is a common organizing signal regulating diverse components of the mammalian urogenital system. 相似文献
60.