A non-catalytic function of Rev1 in translesion DNA synthesis and mutagenesis is mediated by its stable interaction with Rad5

DNA damage tolerance consisting of template switching and translesion synthesis is a major cellular mechanism in response to unrepaired DNA lesions during replication. The Rev1 pathway constitutes the major mechanism of translesion synthesis and base damage-induced mutagenesis in model cell systems. Rev1 is a dCMP transferase, but additionally plays non-catalytic functions in translesion synthesis. Using the yeast model system, we attempted to gain further insights into the non-catalytic functions of Rev1. Rev1 stably interacts with Rad5 (a central component of the template switching pathway) via the C-terminal region of Rev1 and the N-terminal region of Rad5. Supporting functional significance of this interaction, both the Rev1 pathway and Rad5 are required for translesion synthesis and mutagenesis of 1,N⁶-ethenoadenine. Furthermore, disrupting the Rev1–Rad5 interaction by mutating Rev1 did not affect its dCMP transferase, but led to inactivation of the Rev1 non-catalytic function in translesion synthesis of UV-induced DNA damage. Deletion analysis revealed that the C-terminal 21-amino acid sequence of Rev1 is uniquely required for its interaction with Rad5 and is essential for its non-catalytic function. Deletion analysis additionally implicated a C-terminal region of Rev1 in its negative regulation. These results show that a non-catalytic function of Rev1 in translesion synthesis and mutagenesis is mediated by its interaction with Rad5.     

虎耳草不同光温条件下光合特性及有效成分含量的相关性分析

对盆栽的虎耳草进行不同光照和温度处理,测定其叶片光合特性及重要有效成分,分析虎耳草有效成分与光合速率的相关性。结果表明：虎耳草光合速率在透光率50%的条件下最高,透光率100%下最低;100%光照下的岩白菜素和没食子酸的含量最高。光合速率在20℃/16℃（12 h昼/12 h夜）适温下最高,此时岩白菜素和没食子酸的含量也最高。相关性分析表明,温度处理条件下的岩白菜素和没食子酸含量与光合速率、气孔张开面积等呈正相关,其中没食子酸含量与光合速率及蒸腾速率的正相关达极显著;不同光照条件下,没食子酸含量与光合有效辐射呈显著正相关。     

某部入伍新兵心理卫生服务状况及需求调查

目的：调查入伍新兵心理卫生服务状况及对心理卫生服务的需求。方法：采用质性研究和量性研究相结合的方法,对42名入伍新兵作半结构性访谈,编制《部队心理卫生服务状况和需求》调查表,采用调查表对1609名新兵进行调查。结果：9．4％的新兵心理健康知识比较了解;接近半数新兵（43．5％）对心理健康知识很感兴趣;新兵获取心理知识的途径依次为网络、心理知识讲座、报刊杂志、广播电视、面对面咨询;新兵最期望获得的心理知识依次为如何调节自己的情绪、如何建立良好的人际关系、如何塑造自己的个性和常见的心理障碍表现;新兵认为最有帮助的心理服务形式依次为一对一心理咨询、心理知识讲座、心理测验和团体心理活动;只有7．7％的新兵接受过心理卫生服务。结论：新兵对心理健康知识感兴趣,但认知度和利用度均较低,应通过网络、心理知识讲座等方式普及新兵需要的心理健康知识。     

Metabarcoding技术在植物鉴定和多样性研究中的应用

目前植物学研究已进入后植物志时代,iFlora的实现需要以传统植物分类学及相关研究为基础,整合基于高通量测序的DNA条形码（DNAbareoding）技术并开发便携式快速鉴定仪器及构建信息平台。传统植物鉴定多基于形态学分类,而近年来快速发展的DNA条形码快速鉴定技术被各界分类学家认可,在植物鉴定中也被广泛应用。但DNA条形码技术仍存在一些问题亟待解决,如种的鉴定需要多个条形码的解析、Sanger测序平台无法处理混合样品。本文介绍了传统植物分类技术和DNA条形码技术在植物研究中的应用和遇到的瓶颈;并重点介绍了基于高通量测序的metabarcoding技术在植物鉴定及多样性研究中的应用及前景,及其与iFlora的关系。     

食品微生物学实验课立体化教学体系的构建

通过对食品微生物学实验课教学内容、教学手段和方法以及考核方法等方面进行改革,构建了一套基础性实验—综合提高性实验—创新性实验的立体化实验教学体系,使其教学系统化、课程层次化、内容多样化,使学生的综合能力得到了良好的训练,培养了创新意识和综合实验能力,达到了较好的教学效果.     

淡足侧沟茧蜂寄生对甜菜夜蛾幼虫血淋巴总糖、蛋白质及虫体脂质含量的影响

为探讨淡足侧沟茧蜂Microplitis pallidipes Szepligeti调控寄主的生理机制,测定了淡足侧沟茧蜂寄生后,甜菜夜蛾Spodoptera exigua(Hübner)幼虫血淋巴总糖、蛋白质及虫体脂类含量的变化。研究结果表明,寄生后连续5 d的观察时间内,甜菜夜蛾幼虫血淋巴总糖含量从寄生后第1天开始就高于未寄生寄主幼虫,且在寄生后第2至5天达到显著水平;除寄生后第3天外,被寄生寄主幼虫血淋巴总蛋白质含量始终低于未寄生幼虫,且在寄生后第1、4、5天达到显著水平;甜菜夜蛾被寄生后,虫体脂质含量始终高于未寄生幼虫,且从寄生后第2天开始达到显著水平。本研究揭示,淡足侧沟茧蜂寄生刺激了寄主甜菜夜蛾幼虫血淋巴总糖合成,但抑制了其蛋白质合成,同时也刺激了甜菜夜蛾虫体脂质合成。     

Assessment of Microbiome Variation During the Perioperative Period in Liver Transplant Patients: a Retrospective Analysis

Understanding the composition of the microbial populations in the intestines of liver transplant patients is important to preventing postoperative infection. We investigated the relationship between the risk of postoperative infection and variation in the predominant fecal microbial composition during the perioperative period. We prospectively analyzed the predominant intestinal microbiome of five asymptomatic adult carriers of hepatitis B virus (as controls without any antibiotics) at four weekly follow-up visits and 12 patients before operation and at three weekly postoperative follow-up visits within the first month. Analysis was by denaturing gradient gel electrophoresis (DGGE) and sequencing with digital processing of DGGE profiles using BioNumerics software. Our results showed that the predominant intestinal microbial diversity decreased substantially in eight patients during the perioperative period. Among these, five patients experienced infection with a postoperative hospital stay of more than 30 days. The rest of the four patients who experienced shorter postoperative hospital stays showed only slight variation in predominant intestinal bacterial composition and temporal stability similar to asymptomatic controls. Postoperative fecal DGGE profiles showed mostly bands assigned to Bacteroides and Firmicutes. We conclude that an empiric prophylaxis strategy that destructs gut microecological balance will not be effective in reducing the risk of postoperative infection. Instead, the destruction of intestinal microbiota might result in the appearance of opportunistic pathogens such as Bifidobacterium dentium which rarely appears in the intestinal DGGE profiles of normal humans. Cognizance of the variation of intestinal microbial profiles during the perioperative period is a critical aspect of caring for liver transplant recipients.     

Identification of the quantitative trait loci (QTL) underlying water soluble protein content in soybean

Water soluble protein content (SPC) plays an important role in the functional efficacy of protein in food products. Therefore, for the identification of quantitative trait loci (QTL) associated with SPC, 212 F_2:9 lines of the recombinant inbred line (RIL) population derived from the cross of ZDD09454 × Yudou12 were grown along with the parents, in six different environments (location × year) to determine inheritance and map solubility-related genes. A linkage map comprising of 301 SSR markers covering 3,576.81 cM was constructed in the RIL population. Seed SPC was quantified with a macro-Kjeldahl procedure in samples collected over multiple years from three locations (Nantong in 2007 and 2008, Zhengzhou in 2007 and 2008, and Xinxiang in 2008 and 2009). SPC demonstrated transgressive segregation, indicating a complementary genetic structure between the parents. Eleven putative QTL were associated with SPC explaining 4.5–18.2 % of the observed phenotypic variation across the 6 year/location environments. Among these, two QTL (qsp8-4, qsp8-5) near GMENOD2B and Sat_215 showed an association with SPC in multiple environments, suggesting that they were key QTL related to protein solubility. The QTL × environment interaction demonstrated the complex genetic mechanism of SPC. These SPC-associated QTL and linked markers in soybean will provide important information that can be utilized by breeders to improve the functional quality of soybean varieties.     

氢化物发生—原子荧光光谱法测定桉树叶、皮、躯干、根中硒含量

以铁氰化钾为掩蔽剂,1.5% KBH4为还原剂,10%的盐酸为载流液,微波消解处理样品,氢化物发生-原子荧光法(HG-AFS)分别测定桉树叶、皮、躯干和根中硒元素含量。加标回收验证了结果的准确性。考察了仪器测定硒的检出限。并对铁氰化钾,聚环氧琥珀酸(PESA),酒石酸,柠檬酸,乙二胺五种掩蔽剂对11种常见干扰元素的掩蔽效果进行了探究,为优良掩蔽剂的选择提供了参考性资料。     

宽口光唇鱼微卫星位点的筛选与特征分析

通过FIASCO法(Fast Isolation by AFLP Sequences Containing Repeats)筛选宽口光唇鱼Acrossocheilus monticola(Günter)基因组微卫星位点,利用生物素标记的寡核苷酸探针(AC)8、(CT)8、(GGT)8、(GATA)8和(GATT)7首次成功构建宽口光唇鱼基因组微卫星富集文库。从文库中共筛选495个克隆测序,成功设计163对微卫星引物,经PCR扩增检测,获得了18个多态性微卫星标记。利用这18对多态性引物,分析了赤水河赤水市宽口光唇鱼种群的遗传多样性,数据显示:18对多态性微卫星引物的等位基因数为8～31个,平均等位基因数为19.6个,平均期望杂合度为0.8701,平均观测杂合度为0.8312,其中引物Am07、Am35、Am47、Am69、Am78和Am127显著偏离哈迪温伯格平衡(P<0.05),以上数据表明赤水河赤水市宽口光唇鱼种群的遗传多样性水平较高。筛选的微卫星标记对于宽口光唇鱼的遗传背景分析和生物种质资源的保护有重要作用。