全文获取类型
收费全文 | 769篇 |
免费 | 82篇 |
出版年
2021年 | 8篇 |
2020年 | 5篇 |
2018年 | 9篇 |
2017年 | 8篇 |
2016年 | 16篇 |
2015年 | 20篇 |
2014年 | 18篇 |
2013年 | 35篇 |
2012年 | 43篇 |
2011年 | 48篇 |
2010年 | 22篇 |
2009年 | 21篇 |
2008年 | 41篇 |
2007年 | 36篇 |
2006年 | 32篇 |
2005年 | 32篇 |
2004年 | 26篇 |
2003年 | 26篇 |
2002年 | 28篇 |
2001年 | 19篇 |
2000年 | 21篇 |
1999年 | 16篇 |
1998年 | 11篇 |
1997年 | 8篇 |
1995年 | 14篇 |
1994年 | 5篇 |
1993年 | 5篇 |
1992年 | 12篇 |
1991年 | 13篇 |
1990年 | 15篇 |
1989年 | 11篇 |
1988年 | 12篇 |
1987年 | 8篇 |
1986年 | 9篇 |
1985年 | 10篇 |
1983年 | 10篇 |
1982年 | 9篇 |
1979年 | 5篇 |
1976年 | 8篇 |
1975年 | 9篇 |
1974年 | 7篇 |
1973年 | 5篇 |
1972年 | 9篇 |
1971年 | 6篇 |
1970年 | 7篇 |
1969年 | 6篇 |
1968年 | 5篇 |
1966年 | 6篇 |
1965年 | 5篇 |
1945年 | 5篇 |
排序方式: 共有851条查询结果,搜索用时 684 毫秒
101.
Suzanne S. Stokes Robert Albert Ed T. Buurman Beth Andrews Adam B. Shapiro Oluyinka M. Green Andrew R. McKenzie Ludovic R. Otterbein 《Bioorganic & medicinal chemistry letters》2012,22(23):7019-7023
A previously described aryl sulfonamide series, originally found through HTS, targets GlmU, a bifunctional essential enzyme involved in bacterial cell wall synthesis. Using structure-guided design, the potency of enzyme inhibition was increased in multiple isozymes from different bacterial species. Unsuitable physical properties (low Log D and high molecular weight) of those compounds prevented them from entering the cytoplasm of bacteria and inhibiting cell growth. Further modifications described herein led to compounds that possessed antibacterial activity, which was shown to occur through inhibition of GlmU. The left-hand side amide and the right-hand side sulfonamides were modified such that enzyme inhibitory activity was maintained (IC50 <0.1 μM against GlmU isozymes from Gram-negative organisms), and the lipophilicity was increased giving compounds with Log D ?1 to 3. Antibacterial activity in an efflux-pump deficient mutant of Haemophilus influenzae resulted for compounds such as 13. 相似文献
102.
LMO4 is a broadly expressed LIM-only protein that is involved in neural tube development and implicated in breast cancer.
Here we report backbone and side chain NMR assignments for an engineered intramolecular complex of the N-terminal LIM domain
from LMO4 tethered to residues 641–685 of C-terminal binding protein interacting protein (CtIP/RBBP8). 相似文献
103.
Quantifying tetrodotoxin (TTX) has been a challenge in both ecological and medical research due to the cost, time and training required of most quantification techniques. Here we present a modified Competitive Inhibition Enzymatic Immunoassay for the quantification of TTX, and to aid researchers in the optimization of this technique for widespread use with a high degree of accuracy and repeatability. 相似文献
104.
Behrmann E Tao G Stokes DL Egelman EH Raunser S Penczek PA 《Journal of structural biology》2012,177(2):302-313
We present a major revision of the iterative helical real-space refinement (IHRSR) procedure and its implementation in the SPARX single particle image processing environment. We built on over a decade of experience with IHRSR helical structure determination and we took advantage of the flexible SPARX infrastructure to arrive at an implementation that offers ease of use, flexibility in designing helical structure determination strategy, and high computational efficiency. We introduced the 3D projection matching code which now is able to work with non-cubic volumes, the geometry better suited for long helical filaments, we enhanced procedures for establishing helical symmetry parameters, and we parallelized the code using distributed memory paradigm. Additional features include a graphical user interface that facilitates entering and editing of parameters controlling the structure determination strategy of the program. In addition, we present a novel approach to detect and evaluate structural heterogeneity due to conformer mixtures that takes advantage of helical structure redundancy. 相似文献
105.
106.
107.
Henderson R Sali A Baker ML Carragher B Devkota B Downing KH Egelman EH Feng Z Frank J Grigorieff N Jiang W Ludtke SJ Medalia O Penczek PA Rosenthal PB Rossmann MG Schmid MF Schröder GF Steven AC Stokes DL Westbrook JD Wriggers W Yang H Young J Berman HM Chiu W Kleywegt GJ Lawson CL 《Structure (London, England : 1993)》2012,20(2):205-214
This Meeting Review describes the proceedings and conclusions from the inaugural meeting of the Electron Microscopy Validation Task Force organized by the Unified Data Resource for 3DEM (http://www.emdatabank.org) and held at Rutgers University in New Brunswick, NJ on September 28 and 29, 2010. At the workshop, a group of scientists involved in collecting electron microscopy data, using the data to determine three-dimensional electron microscopy (3DEM) density maps, and building molecular models into the maps explored how to assess maps, models, and other data that are deposited into the Electron Microscopy Data Bank and Protein Data Bank public data archives. The specific recommendations resulting from the workshop aim to increase the impact of 3DEM in biology and medicine. 相似文献
108.
Sonya B Dumanis Kelly A Chamberlain Yoo Jin Sohn Young Jin Lee Suzanne Y Guénette Toshiharu Suzuki Paul M Mathews Daniel TS Pak G William Rebeck Yoo-hun Suh Hee-Sae Park Hyang-Sook Hoe 《Molecular neurodegeneration》2012,7(1):1-15
Background
Loss-of-function mutations in PTEN-induced kinase 1 (PINK1) have been linked to familial Parkinson??s disease, but the underlying pathogenic mechanism remains unclear. We previously reported that loss of PINK1 impairs mitochondrial respiratory activity in mouse brains.Results
In this study, we investigate how loss of PINK1 impairs mitochondrial respiration using cultured primary fibroblasts and neurons. We found that intact mitochondria in PINK1?/? cells recapitulate the respiratory defect in isolated mitochondria from PINK1?/? mouse brains, suggesting that these PINK1?/? cells are a valid experimental system to study the underlying mechanisms. Enzymatic activities of the electron transport system complexes are normal in PINK1?/? cells, but mitochondrial transmembrane potential is reduced. Interestingly, the opening of the mitochondrial permeability transition pore (mPTP) is increased in PINK1?/? cells, and this genotypic difference between PINK1?/? and control cells is eliminated by agonists or inhibitors of the mPTP. Furthermore, inhibition of mPTP opening rescues the defects in transmembrane potential and respiration in PINK1?/? cells. Consistent with our earlier findings in mouse brains, mitochondrial morphology is similar between PINK1?/? and wild-type cells, indicating that the observed mitochondrial functional defects are not due to morphological changes. Following FCCP treatment, calcium increases in the cytosol are higher in PINK1?/? compared to wild-type cells, suggesting that intra-mitochondrial calcium concentration is higher in the absence of PINK1.Conclusions
Our findings show that loss of PINK1 causes selective increases in mPTP opening and mitochondrial calcium, and that the excessive mPTP opening may underlie the mitochondrial functional defects observed in PINK1?/? cells. 相似文献109.
Zhou W Newcomb DC Moore ML Goleniewska K O'Neal JF Peebles RS 《Journal of immunology (Baltimore, Md. : 1950)》2008,181(8):5360-5367
Immune sensitization and memory generation are required for the development of allergic inflammation. Our previous studies demonstrate that the cyclooxygenase (COX) metabolic pathway is actively involved in allergic responses and COX inhibition increases allergic airway inflammation in a STAT6-independent fashion. To test the hypothesis that COX inhibition augments allergic inflammation by enhancing immune sensitization and memory, we sensitized STAT6 knockout mice with an i.p. injection of OVA with aluminum hydroxide as an adjuvant and treated the mice with the COX inhibitor indomethacin or vehicle for analyses of the primary and memory immune responses. We found that COX inhibition during immune sensitization, but not the allergic challenge phase, was necessary and sufficient to increase allergic inflammation. COX inhibition during sensitization increased the numbers of mature dendritic cells and activated CD4 T cells in the spleen and augmented OVA-specific IL-5 and IL-13 responses of the splenic CD4 T cells at day 5 after sensitization. COX inhibition during sensitization also augmented allergic Th2 response to OVA challenge 90 days after the sensitization. Therefore, COX inhibition during allergic sensitization augments allergic responses by enhancing Th2 cell activation and memory generation and the proallergic effect is STAT6-independent. These findings provide a mechanistic explanation for the increased allergic inflammation previously shown in the mice treated with COX inhibitors and in COX-deficient mice and suggest that use of COX-inhibiting drugs during initial allergen exposure may increase the risk of developing allergic responses. 相似文献
110.
Troedsson C Lee RF Walters T Stokes V Brinkley K Naegele V Frischer ME 《Applied and environmental microbiology》2008,74(14):4346-4353
Recently, we described a novel denaturing high-performance liquid chromatography (DHPLC) approach useful for initial detection and identification of crustacean parasites. Because this approach utilizes general primers targeted to conserved regions of the 18S rRNA gene, a priori genetic sequence information on eukaryotic parasites is not required. This distinction provides a significant advantage over specifically targeted PCR assays that do not allow for the detection of unknown or unsuspected parasites. However, initial field evaluations of the DHPLC assay suggested that because of PCR-biased amplification of dominant host genes it was not possible to detect relatively rare parasite genes in infected crab tissue. Here, we describe the use of a peptide nucleic acid (PNA) PCR hybridization blocking probe in association with DHPLC (PNA-PCR DHPLC) to overcome inherent PCR bias associated with amplification of rare target genes by use of generic primers. This approach was utilized to detect infection of blue crabs (Callinectes sapidus) by the parasitic dinoflagellate Hematodinium sp. Evaluation of 76 crabs caught in Wassaw Sound, GA, indicated a 97% correspondence between detection of the parasite by use of a specific PCR diagnostic assay and that by use of PNA-PCR DHPLC. During these studies, we discovered one crab with an association with a previously undescribed protist symbiont. Phylogenetic analysis of the amplified symbiont 18S rRNA gene indicated that it is most closely related to the free-living kinetoplastid parasite Procryptobia sorokini. To our knowledge, this is the first report of this parasite group in a decapod crab and of this organism exhibiting a presumably parasitic life history. 相似文献