First insight into personality traits in Northern pike (Esox lucius) larvae: a basis for behavioural studies of early life stages

Environmental Biology of Fishes - Personality traits have been studied for some decades in fish species. Yet, most often, studies focused on juveniles or adults. Thus, very few studies tried to...     

Effect of season and duration of FSH treatment on embryo production in sheep

Thirty-six mature Manchega ewes were used in two experiments to determine the effect of season and of 2- or 3-d FSHp treatment on the ovulation rate and number of transferable embryos produced. During the breeding season, estrus was synchronized with FGA (30 mg for 13 d). Begining 48 or 24 h before sponge removal, each ewe received two daily injections of 4-4-3-3-1-1 or 5-5-3-3 mg of FSHp. Concurrently with the two last injections both groups were administered 100 mug of LH. Ewes were tested for estrus and 6 or 7 d later were laparotomized and surgically flushed to recover embryos. The number of corpora lutea (CL), the total number of embryos and of viable embryos were recorded. Six months later (nonbreeding season) the design was repeated, with each ewe receiving the opposite treatment to that received in the fall. Response in ovulation rate and number of viable embryos did not differ between seasons. Mean (SEM) numbers of observed CL and embryos recovered were higher (P<0.001) with the 3-d treatment (8.7+/-5.8 and 7+/-4.8) than with the 2-d treatment (5.8+/-3.2 and 4.4+/-3) when pooled over the two seasons. The mean number of transferable embryos was higher (P<0.01) with the 3-d (4.2+/-3.9) than with the 2-d treatment (2.5+/-2.3).     

Increased transport of acetyl‐CoA into the endoplasmic reticulum causes a progeria‐like phenotype

The membrane transporter AT‐1/SLC33A1 translocates cytosolic acetyl‐CoA into the lumen of the endoplasmic reticulum (ER), participating in quality control mechanisms within the secretory pathway. Mutations and duplication events in AT‐1/SLC33A1 are highly pleiotropic and have been linked to diseases such as spastic paraplegia, developmental delay, autism spectrum disorder, intellectual disability, propensity to seizures, and dysmorphism. Despite these known associations, the biology of this key transporter is only beginning to be uncovered. Here, we show that systemic overexpression of AT‐1 in the mouse leads to a segmental form of progeria with dysmorphism and metabolic alterations. The phenotype includes delayed growth, short lifespan, alopecia, skin lesions, rectal prolapse, osteoporosis, cardiomegaly, muscle atrophy, reduced fertility, and anemia. In terms of homeostasis, the AT‐1 overexpressing mouse displays hypocholesterolemia, altered glycemia, and increased indices of systemic inflammation. Mechanistically, the phenotype is caused by a block in Atg9a‐Fam134b‐LC3β and Atg9a‐Sec62‐LC3β interactions, and defective reticulophagy, the autophagic recycling of the ER. Inhibition of ATase1/ATase2 acetyltransferase enzymes downstream of AT‐1 restores reticulophagy and rescues the phenotype of the animals. These data suggest that inappropriately elevated acetyl‐CoA flux into the ER directly induces defects in autophagy and recycling of subcellular structures and that this diversion of acetyl‐CoA from cytosol to ER is causal in the progeria phenotype. Collectively, these data establish the cytosol‐to‐ER flux of acetyl‐CoA as a novel event that dictates the pace of aging phenotypes and identify intracellular acetyl‐CoA‐dependent homeostatic mechanisms linked to metabolism and inflammation.     

CO2 evasion from boreal lakes: Revised estimate,drivers of spatial variability,and future projections

Lakes (including reservoirs) are an important component of the global carbon (C) cycle, as acknowledged by the fifth assessment report of the IPCC. In the context of lakes, the boreal region is disproportionately important contributing to 27% of the worldwide lake area, despite representing just 14% of global land surface area. In this study, we used a statistical approach to derive a prediction equation for the partial pressure of CO₂ (pCO₂) in lakes as a function of lake area, terrestrial net primary productivity (NPP), and precipitation (r² = .56), and to create the first high‐resolution, circumboreal map (0.5°) of lake pCO₂. The map of pCO₂ was combined with lake area from the recently published GLOWABO database and three different estimates of the gas transfer velocity k to produce a resulting map of CO₂ evasion (FCO₂). For the boreal region, we estimate an average, lake area weighted, pCO₂ of 966 (678–1,325) μatm and a total FCO₂ of 189 (74–347) Tg C year^?1, and evaluate the corresponding uncertainties based on Monte Carlo simulation. Our estimate of FCO₂ is approximately twofold greater than previous estimates, as a result of methodological and data source differences. We use our results along with published estimates of the other C fluxes through inland waters to derive a C budget for the boreal region, and find that FCO₂ from lakes is the most significant flux of the land‐ocean aquatic continuum, and of a similar magnitude as emissions from forest fires. Using the model and applying it to spatially resolved projections of terrestrial NPP and precipitation while keeping everything else constant, we predict a 107% increase in boreal lake FCO₂ under emission scenario RCP8.5 by 2100. Our projections are largely driven by increases in terrestrial NPP over the same period, showing the very close connection between the terrestrial and aquatic C cycle.     

A Methodical Approach for Systematic Life Cycle Assessment of Wood‐Based Furniture

Existing life cycle assessment (LCA) studies for furniture focus on single pieces of furniture and use a bottom‐up approach based on their bill of materials (BOM) to build up the data inventories. This approach does not ensure completeness regarding material and energy fluxes and representativeness regarding the product portfolio. Integrating material and energy fluxes collected at company level into product LCA (top‐down approach) over‐rides this drawback. This article presents a method for systematic LCA of industrially produced furniture that merges the top‐down approach and bottom‐up approach. The developed method assigns data collected at the company level to the different products while, at the same time, considering that wood‐based furniture is a complex product. Hence, several classifications to reduce the complexity to a manageable level have been developed. Simultaneously, a systematic calculation routine was established. The practical implementation of the developed method for systematic LCA is carried out in a case study within the German furniture industry. The system boundary was set in accord with the EN 15804 specification cradle‐to‐gate‐with‐options. The analysis therefore includes the manufacturing phase supplemented by an end‐of‐life scenario. The case study shows that the manufacturing of semifinished products (especially wood‐based panels and metal components) as well as the electric energy demand in furniture manufacturing account for a notable share of the environmental impacts. A sensitivity analysis indicates that up to roughly 10% of the greenhouse gas emissions are not recorded when conducting an LCA based on a BOM instead of applying the developed approach.     

Dysbiosis-Associated Change in Host Metabolism Generates Lactate to Support Salmonella Growth

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Inverse relationship of epilithic algae and pelagic phosphorus in unproductive lakes: Roles of N2 fixers and light

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