全文获取类型
收费全文 | 109217篇 |
免费 | 9953篇 |
国内免费 | 69篇 |
出版年
2021年 | 1453篇 |
2020年 | 902篇 |
2019年 | 1109篇 |
2018年 | 1429篇 |
2017年 | 1324篇 |
2016年 | 2206篇 |
2015年 | 3694篇 |
2014年 | 4201篇 |
2013年 | 5481篇 |
2012年 | 7025篇 |
2011年 | 7078篇 |
2010年 | 4652篇 |
2009年 | 4190篇 |
2008年 | 5998篇 |
2007年 | 6285篇 |
2006年 | 6104篇 |
2005年 | 5754篇 |
2004年 | 5680篇 |
2003年 | 5459篇 |
2002年 | 5141篇 |
2001年 | 1162篇 |
2000年 | 860篇 |
1999年 | 1297篇 |
1998年 | 1568篇 |
1997年 | 1157篇 |
1996年 | 1031篇 |
1995年 | 981篇 |
1994年 | 936篇 |
1993年 | 1002篇 |
1992年 | 999篇 |
1991年 | 855篇 |
1990年 | 829篇 |
1989年 | 805篇 |
1988年 | 793篇 |
1987年 | 742篇 |
1986年 | 779篇 |
1985年 | 856篇 |
1984年 | 1050篇 |
1983年 | 905篇 |
1982年 | 1123篇 |
1981年 | 1176篇 |
1980年 | 1030篇 |
1979年 | 711篇 |
1978年 | 774篇 |
1977年 | 722篇 |
1976年 | 695篇 |
1975年 | 559篇 |
1974年 | 660篇 |
1973年 | 612篇 |
1972年 | 412篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
51.
52.
53.
54.
Lech Michalczuk Robert S. Bandurski 《Biochemical and biophysical research communications》1980,93(2):588-592
We have demonstrated the in vitro enzymatic synthesis of an ester of indole-3-acetic acid (IAA) and glucose and of IAA and myo-inositol by the following reaction sequence: lt]o| li]1) IAA + UDPG ? IAA-glucose +UDP li]2) IAA-glucose +myo-inositol → IAA-itmyo-inositol +glucose The enzymes were partially purified from extracts of immature kernels of Zea mays sweet corn and the two activities separated on a Sephadex G-150 column. Products were characterized, primarily, by comparison of their 70 eV mass spectra with those of authentic synthetic standards. To our knowledge this is the first example of enzymatically catalyzed acylation by a 1-O-acylsugar. 相似文献
55.
56.
Daily ingestion of iodide alone is not adequate to sustain production of the thyroid hormones, tri- and tetraiodothyronine. Proper maintenance of iodide in vivo also requires its active transport into the thyroid and its salvage from mono- and diiodotyrosine that are formed in excess during hormone biosynthesis. The enzyme iodotyrosine deiodinase responsible for this salvage is unusual in its ability to catalyze a reductive dehalogenation reaction dependent on a flavin cofactor, FMN. Initial characterization of this enzyme was limited by its membrane association, difficult purification and poor stability. The deiodinase became amenable to detailed analysis only after identification and heterologous expression of its gene. Site-directed mutagenesis recently demonstrated that cysteine residues are not necessary for enzymatic activity in contrast to precedence set by other reductive dehalogenases. Truncation of the N-terminal membrane anchor of the deiodinase has provided a soluble and stable source of enzyme sufficient for crystallographic studies. The structure of an enzyme·substrate co-crystal has become invaluable for understanding the origins of substrate selectivity and the mutations causing thyroid disease in humans. 相似文献
57.
Elena C. Guzman Alfonso Jimenez-Sanchez Elisha Orr Robert H. Pritchard 《Molecular & general genetics : MGG》1988,212(2):203-206
Summary A temperature shift-up accompanied by a reduction in RNA polymerase activity in Escherichia coli causes an increased rate of initiation leading to a 1.7- to 2.2-fold increase in chromosome copy number. A temperature shift-up without a reduction in polymerase activity induces only a transient non-scheduled initiation of chromosome replication caused by heat shock with no detectable effect on chromosome copy number. 相似文献
58.
59.
Simon D X Chuong Robert T Mullen Douglas G Muench 《The Journal of biological chemistry》2002,277(4):2419-2429
The control of subcellular mRNA localization and translation is often mediated by protein factors that are directly or indirectly associated with the cytoskeleton. We report the identification and characterization of a rice seed protein that possesses both RNA and microtubule binding activities. In vitro UV cross-linking assays indicated that this protein binds to all mRNA sequences tested, although there was evidence for preferential binding to RNAs that contained A-C nucleotide sequence motifs. The protein was purified to homogeneity using a two-step procedure, and amino acid sequencing identified it as the multifunctional protein (MFP), a peroxisomal enzyme known to possess a number of activities involved in the beta-oxidation of fatty acids. The recombinant version of this rice MFP binds to RNA in UV cross-linking and gel mobility shift experiments, co-sediments specifically with microtubules, and possesses at least two enzymatic activities involved in peroxisomal fatty acid beta-oxidation. Taken together these data suggest that MFP has an important role in mRNA physiology in the cytoplasm, perhaps in regulating the localization or translation of mRNAs through an interaction with microtubules, in addition to its peroxisomal function. 相似文献
60.
Cascade J. B. Sorte Ron J. Etter Robert Spackman Elizabeth E. Boyle Robyn E. Hannigan 《PloS one》2013,8(11)
As the climate warms, species that cannot tolerate changing conditions will only persist if they undergo range shifts. Redistribution ability may be particularly variable for benthic marine species that disperse as pelagic larvae in ocean currents. The blue mussel, Mytilus edulis, has recently experienced a warming-related range contraction in the southeastern USA and may face limitations to northward range shifts within the Gulf of Maine where dominant coastal currents flow southward. Thus, blue mussels might be especially vulnerable to warming, and understanding dispersal patterns is crucial given the species'' relatively long planktonic larval period (>1 month). To determine whether trace elemental “fingerprints” incorporated in mussel shells could be used to identify population sources (i.e. collection locations), we assessed the geographic variation in shell chemistry of blue mussels collected from seven populations between Cape Cod, Massachusetts and northern Maine. Across this ∼500 km of coastline, we were able to successfully predict population sources for over two-thirds of juvenile individuals, with almost 80% of juveniles classified within one site of their collection location and 97% correctly classified to region. These results indicate that significant differences in elemental signatures of mussel shells exist between open-coast sites separated by ∼50 km throughout the Gulf of Maine. Our findings suggest that elemental “fingerprinting” is a promising approach for predicting redistribution potential of the blue mussel, an ecologically and economically important species in the region. 相似文献