排序方式: 共有72条查询结果,搜索用时 264 毫秒
51.
Sindhu R Kuttiraja M Binod P Janu KU Sukumaran RK Pandey A 《Bioresource technology》2011,102(23):10915-10921
The aim of this work was to study the feasibility of using sugarcane tops as feedstock for the production of bioethanol. The process involved the pretreatment using acid followed by enzymatic saccharification using cellulases and the process was optimized for various parameters such as biomass loading, enzyme loading, surfactant concentration and incubation time using Box–Behnken design. Under optimum hydrolysis conditions, 0.685 g/g of reducing sugar was produced per gram of pretreated biomass. The fermentation of the hydrolyzate using Saccharomyces cerevisae produced 11.365 g/L of bioethanol with an efficiency of about 50%. This is the first report on utilization of sugarcane tops for bioethanol production. 相似文献
52.
Organosolvent pretreatment and enzymatic hydrolysis of rice straw for the production of bioethanol 总被引:1,自引:0,他引:1
R Sindhu P Binod KU Janu RK Sukumaran A Pandey 《World journal of microbiology & biotechnology》2012,28(2):473-483
The present study investigates the operational conditions for organosolvent pretreatment and hydrolysis of rice straw. Among
the different organic acids and organic solvents tested, acetone was found to be most effective based on the fermentable sugar
yield. Optimization of process parameters for acetone pretreatment were carried out. The structural changes before and after
pretreatment were investigated by scanning electron microscopy, X-ray diffraction and Fourier transform infrared (FTIR) analysis.
The X-ray diffraction profile showed that the degree of crystallinity was higher for acetone pretreated biomass than that
of the native. FTIR spectrum also exhibited significant difference between the native and pretreated samples. Under optimum
pretreatment conditions 0.458 g of reducing sugar was produced per gram of pretreated biomass with a fermentation efficiency
of 39%. Optimization of process parameters for hydrolysis such as biomass loading, enzyme loading, surfactant concentration
and incubation time was done using Box–Benhken design. The results indicate that acetone pretreated rice straw can be used
as a good feed stock for bioethanol production. 相似文献
53.
Saveetha Kandasamy Karthiba Loganathan Raveendran Muthuraj Saravanakumar Duraisamy Suresh Seetharaman Raguchander Thiruvengadam Balasubramanian Ponnusamy Samiyappan Ramasamy 《Proteome science》2009,7(1):1-8
Background
Heat stress (HS) and related illnesses are a major concern in military, sports, and fire brigadiers. HS results in physiologic responses of increased temperature, heart rate and sweating. In heat stroke, inflammatory response plays an important role and it is evidenced that turpentine (T) induced circulating inflammatory cytokines reduced survival rate and duration at 42°C. Here we report the alteration in the protein expression in liver cells upon HS with and without T treatment using two dimensional gel electrophoresis (2-DE), tryptic in-gel digestion and MALDI-TOF-MS/MS approaches 相似文献54.
Sanju Kumari Jennifer M. Sheba Maheshwaran Marappan Shanmugasunderam Ponnuswamy Suresh Seetharaman Nagarajan Pothi Mohankumar Subbarayalu Raveendran Muthurajan Senthil Natesan 《Molecular biotechnology》2010,46(1):63-71
Brown planthopper (Nilaparvata lugens St?l) is one of the major insect pests of rice. A Sri Lankan indica rice cultivar Rathu Heenati was found to be resistant to all biotypes of the brown planthopper. In the present study, a total
of 268 F7 RILs of IR50 and Rathu Heenati were phenotyped for their level of resistance against BPH by the standard seedbox screening
test (SSST) in the greenhouse. A total of 53 SSR primers mapped on the chromosome 3 were used to screen the polymorphism between
the parents IR50 and Rathu Heenati, out of which eleven were found to be polymorphic between IR50 and Rathu Heenati. The eleven
primers that have shown polymorphism between the IR50 and Rathu Heenati parents were genotyped in a set of five resistant
RILs and five susceptible RILs along with the parents for co-segregation analysis. Among the eleven primers, two primers namely
RM3180 (18.22 Mb) and RM2453 (20.19 Mb) showed complete co-segregation with resistance. The identification of SSR markers
linked with BPH resistant could be used for the maker assisted selection (MAS) program in rice breeding and to map the resistant
genes on rice chromosomes for further gene cloning. 相似文献
55.
Raveendran Karthika Vaiyapuri Murugadas Benala Manikantha Sivam Visnuvinayagam Badireddy Madhusudana Rao 《International microbiology》2023,26(3):459-469
International Microbiology - The emergence of multidrug-resistant (MDR) E. coli with deleterious consequences to the health of humans and animals has been attributed to the inappropriate use of... 相似文献
56.
Raveendran M Harris RA Milosavljevic A Johnson Z Shelledy W Cameron J Rogers J 《Genomics》2006,88(6):706-710
Identification of polymorphic microsatellite loci in nonhuman primates is useful for various biomedical and evolutionary studies of these species. Prior methods for identifying microsatellites in nonhuman primates are inefficient. We describe a new strategy for marker development that uses the available whole genome sequence for rhesus macaques. Fifty-four novel rhesus-derived microsatellites were genotyped in large pedigrees of rhesus monkeys. Linkage analysis was used to place 51 of these loci into the existing rhesus linkage map. In addition, we find that microsatellites identified this way are polymorphic in other Old World monkeys such as baboons. This approach to marker development is more efficient than previous methods and produces polymorphisms with known locations in the rhesus genome assembly. Finally, we propose a nomenclature system that can be used for rhesus-derived microsatellites genotyped in any species or for novel loci derived from the genome sequence of any nonhuman primate. 相似文献
57.
Effect of water-soluble fraction of cigarette smoke on human aortic endothelial cells – a proteomic approach 总被引:1,自引:0,他引:1
Raveendran M Senthil D Utama B Shen Y Wang J Zhang Y Wang XL 《Cell biology and toxicology》2005,21(1):27-40
Proteomic analysis is an important investigative tool used to systematically explore cellular proteins that are responsive to adverse environmental challenges. Tobacco smoking is the second major cause of death in the world. In this study, we utilized two-dimensional electrophoresis (2-DE) and mass spectrometry (MS) technologies to explore protein changes in human aortic endothelial cells (HAECs) in response to cigarette smoke extracts (CSE). Among 389 individual proteins resolved using 2-DE, 43 had a 2- to 3-fold change in levels as measured by spot intensity and 32 had more than a 3-fold change. Sixteen of the 32 spots with sufficient amount of proteins were excised for identification by performing matrix-assisted laser desorption/ionization (MALDI)-MS analysis. Using a peptide mass fingerprinting (PMF) to search the nrNCBI database, we identified all these 16 proteins, which were either increased (n = 9) or decreased (n = 7) after CSE treatment. All these proteins have known functions, however, none have been reported to be altered after CSE treatment. The findings from our study suggest that utilizing a systemic investigative tool, such as the proteomic approach using 2-DE, may play an important role in discovering novel molecular mechanisms for cigarette smoking-induced pathological changes. Further investigation following the systemic discoveries must be further examined as they may potentially lead to new therapeutic approaches to smoking-induced diseases – a health issue affecting everyone in the world. 相似文献
58.
59.
Gregg W.C. Thomas Richard J. Wang Arthi Puri R. Alan Harris Muthuswamy Raveendran Daniel S.T. Hughes Shwetha C. Murali Lawrence E. Williams Harsha Doddapaneni Donna M. Muzny Richard A. Gibbs Christian R. Abee Mary R. Galinski Kim C. Worley Jeffrey Rogers Predrag Radivojac Matthew W. Hahn 《Current biology : CB》2018,28(19):3193-3197.e5
60.
A. Karthikeyan M. Sudha N. Senthil M. Pandiyan M. Raveendran P. Nagrajan 《Archives Of Phytopathology And Plant Protection》2013,46(6):712-716
Bulk segregant analysis (BSA) and random amplified polymorphic DNA (RAPD) techniques were used to analyse the F2 individuals of susceptible VBN (Gg) 2 × resistant KMG 189 to screen and identify the molecular marker linked to mungbean yellow mosaic virus (MYMV) resistant gene in mungbean. Two DNA bulks namely resistant bulks and susceptible bulks were setup by pooling equal amount of DNA from five randomly selected plants of each disease response. A total of 72 random sequence decamer oligonucleotide primers were used for RAPD analysis. Primer OPBB 05 (5′-GGGCCGAACA-3′) generated OPBB 05 260 fragment in resistant parent and their bulks but not in the susceptible parent and their bulks. Co segregation analysis was performed in resistant and susceptible F2 individuals, it confirmed that OPBB 05 260 marker was tightly linked to mungbean yellow mosaic virus resistant gene in mungbean. 相似文献