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排序方式: 共有131条查询结果,搜索用时 31 毫秒
121.
Physical exercise-induced hypoglycemia caused by failed silencing of monocarboxylate transporter 1 in pancreatic beta cells 下载免费PDF全文
122.
The present study deals with the presence of Diopatra
marocensis in the eastern Mediterranean. This species is small-sized and inhabited muddy bottom near the opening of rivers or lagoons [salinity range: 33−39‰] in the Aegean and Levantine Seas, and reached a maximum density of 90 ind.m-2 in Mersin Bay. This species might be an alien species that was introduced from the East Atlantic (near Gibraltar) to the eastern Mediterranean via ballast water of ships, as it has never been reported from the western Mediterranean Sea. 相似文献
123.
Cemalettin Alp Deniz Ekinci Mehmet Serdar Gültekin Murat Şentürk Ertan Şahin Ömer İrfan Küfrevioğlu 《Bioorganic & medicinal chemistry》2010,18(12):4468-4474
Here we propose a novel one-pot synthesis of new tosyl-pyrrole derivatives. By means of the new developed method, pyrrole derivatives were synthesized at room temperature in a single step, and a useful method is proposed for the synthesis of similar compounds. Moreover, inhibitions of two human cytosolic carbonic anhydrase (hCA, EC 4.2.1.1) isozymes I and II by 1-tosyl-pyrrole and 1-tosyl-pyrrol-2-on derivatives were investigated. 1-Tosyl-pyrrole, 1-tosyl-1H-pyrrol-2(5H)-one, 5-hydroxy-1-tosyl-1H-pyrrol-2(5H)-one and 5-oxo-1-tosyl-2,5-dihydro-1H-pyrrol-2-yl acetate showed inhibitory action with Ki values in the range of 14.6–42.4 μM for hCA I and 0.53–37.5 μM for hCA II, respectively. All pyrrole derivatives were competitive inhibitors with 4-nitrophenylacetate as substrate. Some new synthesized pyrrole derivatives showed very effective hCA II inhibitory effects, in the same range as the clinically used sulfonamide acetazolamide, and might be used as leads for generating enzyme inhibitors targeting other CA isoforms. 相似文献
124.
Sarigüllü Ozgüney I Yeşim Karasulu H Kantarci G Sözer S Güneri T Ertan G 《AAPS PharmSciTech》2006,7(4):E39-E45
The aim of this study was to evaluate and compare the in vitro and in vivo transdermal potential of w/o microemulsion (M)
and gel (G) bases for diclofenac sodium (DS). The effect of dimethyl sulfoxide (DMSO) as a penetration enhancer was also examined
when it was added to the M formulation. To study the in vitro potential of these formulations, permeation studies were performed
with Franz diffusion cells using excised dorsal rat skin. To investigate their in vivo performance, a carrageenan-induced
rat paw edema model was used. The commercial formulation of DS (C) was used as a reference formulation. The results of the
in vitro permeation studies and the paw edema tests were analyzed by repeated-measures analysis of variance. The in vitro
permeation studies found that M was superior to G and C and that adding DMSO to M increased the permeation rate. The permeability
coefficients (Kp) of DS from M and M+DMSO were higher (Kp=4.9×10−3±3.6×10−4 cm/h and 5.3×10−3±1.2×10−3 cm/h, respectively) than the Kp of DS from C (Kp=2.7×10−3±7.3×10−4 cm/h) and G (Kp=4.5×10−3±4.5×10−5 cm/h). In the paw edema test, M showed the best permeation and effectiveness, and M+DMSO had nearly the same effect as M.
The in vitro and in vivo studies showed that M could be a new, alternative dosage form for effective therapy. 相似文献
125.
S. Kapicioglu D.B. McNamara M.Y. Vacarella P.J. Kadowitz S. Hoda A. Ertan 《Prostaglandins, leukotrienes, and essential fatty acids》1990,39(4):277-281
Rat gastroduodenal mucosa forms prostaglandin (PG) E2. However, little is known about regional differences in PGE2 formation or the effect of gastric hydrochloric acid (HC1) perfusion on regional PGE2 formation. In this study, the rats were divided into 3 groups. Group 1 received intravenous (i.v.), 1 Ml/h, and intragastric (i.g.), 8 ml/h, perfusions of saline simultaneously for 3 h. Group 2 received saline i.v. and 0.15 N HC1 i.g., 8 ml/h. Group 3 was injected with a bolus of asprin (ASA), 60 mg/kg, followed by ASA, 40 mg/kg/h i.v., and 0.15 N HC1 i.g.. The gastric aspirates were analyzed for volume and pH. Segments of gastroduodenal tissue from the fundus, corpus, antrum, and duodenum were minced and then incubated in 1 ml of 5 mM Tris buffer, pH 8.4, for 30 sec with mixing; the incubate was assayed for PGE2 by radioimmunoassay. Intragastric HC1 decreased the pH of aspirate without producing gastric mucosal lesions. However, when combined with i.v. ASA, ulcer formation was present in all animals (p less than 0.05). PGE2 was formed by isolated tissue from four different gastroduodenal regions. The duodenum formed significantly greater amounts than the fundus, antrum, or corpus, which were similar. Intragastric HC1 produced a trend toward increased PGE2 formation (pmol PGE2/mg tissue) in the fundus, 143 +/- 36 to 237 +/- 57; corpus, 87 +/- 13 to 200 +/- 57; antrum, 157 +/- 28 to 224 +/- 65; and duodenum, 235 +/- 56 to 338 +/- 51. However, statistical significance was not reached. 相似文献
126.
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128.
A. R. Janecke Nils U. Bosshard Ertan Mayatepek Andreas Schulze Richard Gitzelmann Ann Burchell Claus R. Bartram Bart Janssen 《Human genetics》1999,104(3):275-277
Glycogen storage disease type 1 (GSD 1) results from deficiency of the microsomal multicomponent glucose-6-phosphatase system.
Malfunction of the catalytic subunit characterises GSD 1a. GSD 1b and GSD 1c are characterised by defective microsomal glucose-6-phosphate
or pyrophosphate/phosphate transport, respectively. Recently, a gene encoding a microsomal transporter protein has been found
to be mutated in GSD 1b and 1c patients. Here, we report the genomic sequence of the transporter gene and the detection of
a homozygous 2-bp deletion (1211delCT) and a homozygous donor splice site mutation (317+1G→T) in two GSD 1c patients, confirming
that GSD 1c is allelic to GSD 1b.
Received: 16 October 1998 / Accepted: 11 January 1998 相似文献
129.
David M. Hershey Xuefeng Ren Ryan A. Melnyk Patrick J. Browne Ertan Ozyamak Stephanie R. Jones Michelle C. Y. Chang James H. Hurley Arash Komeili 《PLoS biology》2016,14(3)
Many living organisms transform inorganic atoms into highly ordered crystalline materials. An elegant example of such biomineralization processes is the production of nano-scale magnetic crystals in magnetotactic bacteria. Previous studies implicated the involvement of two putative serine proteases, MamE and MamO, during the early stages of magnetite formation in Magnetospirillum magneticum AMB-1. Here, using genetic analysis and X-ray crystallography, we show that MamO has a degenerate active site, rendering it incapable of protease activity. Instead, MamO promotes magnetosome formation through two genetically distinct, noncatalytic activities: activation of MamE-dependent proteolysis of biomineralization factors and direct binding to transition metal ions. By solving the structure of the protease domain bound to a metal ion, we identify a surface-exposed di-histidine motif in MamO that contributes to metal binding and show that it is required to initiate biomineralization in vivo. Finally, we find that pseudoproteases are widespread in magnetotactic bacteria and that they have evolved independently in three separate taxa. Our results highlight the versatility of protein scaffolds in accommodating new biochemical activities and provide unprecedented insight into the earliest stages of biomineralization. 相似文献
130.
Sophie I. Holland Haluk Ertan Kate Montgomery Michael J. Manefield Matthew Lee 《The ISME journal》2021,15(6):1709
Dichloromethane (DCM; CH2Cl2) is a toxic groundwater pollutant that also has a detrimental effect on atmospheric ozone levels. As a dense non-aqueous phase liquid, DCM migrates vertically through groundwater to low redox zones, yet information on anaerobic microbial DCM transformation remains scarce due to a lack of cultured organisms. We report here the characterisation of DCMF, the dominant organism in an anaerobic enrichment culture (DFE) capable of fermenting DCM to the environmentally benign product acetate. Stable carbon isotope experiments demonstrated that the organism assimilated carbon from DCM and bicarbonate via the Wood–Ljungdahl pathway. DCMF is the first anaerobic DCM-degrading population also shown to metabolise non-chlorinated substrates. It appears to be a methylotroph utilising the Wood–Ljungdahl pathway for metabolism of methyl groups from methanol, choline, and glycine betaine. The flux of these substrates from subsurface environments may either directly (DCM, methanol) or indirectly (choline, glycine betaine) affect the climate. Community profiling and cultivation of cohabiting taxa in culture DFE without DCMF suggest that DCMF is the sole organism in this culture responsible for substrate metabolism, while the cohabitants persist via necromass recycling. Genomic and physiological evidence support placement of DCMF in a novel genus within the Peptococcaceae family, ‘Candidatus Formimonas warabiya’.Subject terms: Pollution remediation, Microbial ecology, Soil microbiology, Biogeochemistry 相似文献