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51.
流行性乙型脑炎病毒血清抗体半微量空斑抑制法的改进 总被引:5,自引:0,他引:5
本文报道一种检测流行性乙型脑炎(简称乙脑)中和抗体的简化空斑抑制试验方法,用BHK21传代细胞在24孔塑料板上进行试验,在细胞未形成单层时,将病毒直接接种在刚消化的细胞悬液内,孵育后加甲基纤维素复盖物,再培养,细胞层经染色后即可计算空斑数。 用该法检查47名儿童乙脑疫苗免疫前后的中和抗体,与常规的鸡胚细胞全量法或BHK21传代细胞微量中和试验法作了比较,结果表明本法与其它常规法的敏感性一致,且具有简便快速、节省人力和原材料、空斑形成率高的优点,便于大量血清学检测。 相似文献
52.
新城疫病毒单克隆抗体的特性及应用 总被引:2,自引:0,他引:2
建立了8个分泌抗新城疫病毒(NDV)特异性单克隆抗体(McAb)的杂交瘤细胞株,根据它们的免疫生物学特性可以分为三种类型:(1)具有FA和ELISA特性(FN1、FN4、FN29、FN30、FN35、FNl22);(2)具有FA、ELISA和HI特性(FN7);(3)具有ELISA、HI特性和中和能力(FN106),根据FN30和FN106的ELISA试验,可将11个NDV毒株分为二种不同的抗原群,应用FN4-FITC,FN7-FITC和FN29-HRP试剂,对人工感染NDV和野外送检病例检测结果表明,单抗试剂的DFA阳性率(92.3%)高于病毒分离阳性率(87.2%),两种方法的符合率89.7%,这些单抗试剂用于临床诊断敏感性和特异性高,且方法快速、简便。 相似文献
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54.
X. B. Zheng 《Journal of applied microbiology》1987,62(6):521-525
In April and May 1983, a total of 124 samples of faeces from 124 swine was examined for the presence of Yersinia enterocolitica by a variety of isolation procedures. The organisms were recovered from 48˙4% of the swine, which all had diarrhoea. The strain belonged to serotypes 0: 3 and 0: 9 which are considered to be pathogenic for humans. 相似文献
55.
根癌农杆菌Ti质粒的T区DNA带有致瘤基因,其基因1和基因2编码生长素吲哚乙酸生物合成途径中的两个酶。以pGV 354(pBR322质粒中插有Ti质粒C 58 T区DNA的HindⅢ15—HindⅢ22大片段)重组质粒出发,我们分离了基因1和基因2,并构建了带有卡那霉素抗性基因的重组质粒pBZ 692,通过基因载体pGV 3850,我们将基因1和基因2引入了高等植物。结果证明基因1和基因2能促使烟草、向日葵、土豆等转化组织分化长根,转化的根在MS_0培养基上能脱分化形成愈伤组织并自主生长,在转化的组织中有转化标记胭脂碱的存在。 相似文献
56.
Sixty depressed patients and 52 normal controls completed three selfreport inventories: the Symptom Checklist-90 (SCL-90), the Beck Depression Inventory (BDI) and a new Verbal Style Investigation Schedule (VESIS) developed by the first author. The VESIS uses 16 key emotional and physical terms from Western inventories and identified the words and phrases most commonly used by Chinese patients to express these feeling states. Chinese subjects commonly used the key term itself for only 3 or the 16 key terms; they usually preferred to use other words or phrases to express the feeling state. We categorized these Chinese expressions into four styles of verbal expression: Psychological, Somatic, Neutral (i.e., a mixture of psychological and somatic) and Deficient (i.e., lack of expression because of denial or suppression). Three of the 12 key emotional terms of the VESIS (depressed, fearful, and anxiousness) were more commonly expressed in a somatic or neutral mode than the other key emotional terms. The key terms suicidal interest and being punished were more commonly expressed in a deficient style than other key emotional terms. The somatic factor score of the SCL-90 was not correlated with increased somatic expression of emotional states; thus patients who have multiple somatic complaints are not more likely to express emotions somatically. The hypothesis of somatization is discussed in light of this study. 相似文献
57.
58.
Four experiments were replicated 1) to establish dose-response relationships between lysophosphatidylcholine (LPC), sperm motility, and the acrosome reaction (AR), 2) to evaluate the influence of rabbit serum (RS) on these endpoints, 3) to compare buck differences in induction of the AR, and 4) to examine fertilizing ability in vitro of sperm tested under the first three objectives. Semen was collected from Dutch-belted rabbits, washed once by centrifugation, resuspended, and preincubated for 2 or 4 hr in a chemically defined medium (DM), DM plus 20% RS, or BSA-free DM plus 20% RS at 37°C. At the end of preincubation LPC was added to the preincubated sperm at concentrations of from 0 to 100 μg/ml. Sperm were examined .5–4 hr later for AR and sperm motility. For in vitro fertilization, sperm and ova were coincubated in DM up to 24 hr after insemination and in a more complex medium for another 24 hr. Addition of LPC to 4-hr-preincubated sperm was more effective for inducing the AR than addition to 2-hr-preincubated sperm. A significant increase (P < .05) in the AR occurred in 15 and 30 min following exposure to 100 and 80 μg of LPC per ml, respectively, but the higher concentration of LPC decreased sperm motility. Addition of 20% RS to DM with or without BSA surprisingly inhibited the AR but maintained sperm motility, as expected. Bucks differed (P < .05) in the initial percentage and the induced percentage of AR sperm. For the AR the optimal concentration of LPC per ml was 80 μg, but for in vitro fertilization 60 μg tended to be superior. 相似文献
59.
聚乙二醇处理大豆种子子叶中几种酶活性和可溶性蛋白含量的变化 总被引:5,自引:0,他引:5
聚乙二醇处理的大豆种子的异柠檬酸裂解酶、苹果酸脱氢酶、过氧化氢酶、超氧物歧化酶、酸性磷酸酶、碱性磷酸酶的活性明显高于受低温吸胀冷害的种子子叶的活性,相关的酶活性协同地增长,而蛋白质的含量没有明显的变化。这些酶活性的提高可能是渗透调控处理对细胞膜系统修补的结果。 相似文献
60.
用30—70GyX射线照射小麦幼苗(浸种后5天)后发现根毛区到根尖的距离缩短,根毛变密,根毛长度为对照的2—3倍。照射后2—3天就可看到该现象。根毛着生处到根尖的距离随剂量增加而减少,甚至根尖全为根毛所复盖,另外还看到有分叉的根毛。根尖纵切片表明根尖分生区随剂量增加而缩小,分生区后接着就出现输导组织。玉米和黄瓜也有类似现象。这现象说明根细胞的分裂过程对射线很敏感,而分化过程则相当耐辐射,细胞分裂停止后立即转向细胞分化过程。 相似文献