柱孢鱼腥藻的铁蛋白与棕色固氮菌的钼铁蛋白的交叉互补作用

纯化的柱孢鱼腥藻铁蛋白能够与棕色固氮菌的钼铁蛋白有效地交叉反应,展现较高的活性。此异源交叉反应的乙炔还原比活及放氢比活,分别是蓝藻同源互补比活的83.8及66.7％。比较藻铁蛋白与菌钼铁蛋白异源交叉反应及藻固氮酶组分之间的同源反应的动力学特点时发现,铁蛋白对钼铁蛋白的最佳克分子比数前者(异源交叉反应)较后者(藻同源反应)为高,前者为5,后者为1;但反应的时间进程两者差别不大。     

牛病毒性腹泻病毒单克隆抗体的研究

牛病毒性腹泻——粘膜病是世界性广泛流行的奶牛和肉牛的传染病。其病原为牛病毒性腹泻病毒(BVDV),属于披膜病毒科的瘟病毒属,它的许多生物学特性至今还不很清楚。本试验建立了12株分泌抗BVDV的单克隆抗体(McAb)杂交瘤细胞株,并结合免疫转移电泳法和放射免疫沉淀法,初步研究了BVDV的多肽。     

甘肃裕固族体质特征初步研究

本文调查了甘肃省裕固族成年人409人(男210人,女199人)的体质特征。其中活体测量51项,活体观察8项。结果表明:裕固族既具有黄种人的特征,也有本民族的特点,例如头部较圆较高;面部较长偏狭;鼻子高;鼻梁直,属狭鼻型;身材普遍较高,体质较好。这些特征与华北地区的汉族和达斡尔族,羌族及锡伯族的特征比较接近,而与苗族、黎族较远。     

少年游泳运动员冬训期间HbA_2百分含量的规律性变化

 本研究用聚丙烯酰胺凝胶垂直板电泳,双波长扫描仪对少年游泳运动员的HbA_2百分含量进行测定。发现大运动量训练对HbA_2水平是有影响的。经六天训练(星期二、五为大运动量训练),星期日休息一天,次周的星期一晨HbA_2百分含量升高,星期二大运动量训练后的次日晨HbA_2百分含量显著下降。运动性贫血的运动员,其HbA_2百分含量一周中的变化趋势与正常运动员相似,但HbA_2百分含量比正常运动员高。     

Immunohistochemical localization of neuropeptide Y in the guinea pig medulla oblongata

Summary The immunohistochemical localization of neuropeptide Y (NPY) was correlated with those of dopamine--hydroxylase (DBH) and vasoactive intestinal polypeptide (VIP) by mapping serial 7 m paraffin sections at three levels of the guina pig lower brainstem: a) area postrema, b) dorsal motor nucleus of the vagus, and c) nucleus prepositus of the hypoglossal nerve. Based on differences in transmitter expression, three populations of NPY-immunoreactive (IR) neurons were distinguished: NPY-IR catecholaminergic cells (NPY/CA), NPY-IR VIP-ergic cells (NPY/VIP), and NPY-IR cells which were not reactive to either DBH or VIP. Within these populations, size differences among neurons in characteristic locations allowed differentiation among the following subpopulations: NPY/CA neurons in the lateral reticular nucleus — magnocellular part (mean neuronal size 538 m²) and parvocellular part (318 m²)-, in the vagus-solitarius complex (433 m²), and in the dorsal strip (348 m²); NPY/VIP neurons in the vagus-solitarius complex (368 m²) and in the nucleus ovalis (236 m²). Apart from scattered NPY-IR cell bodies in the regions listed above, NPY-IR cell bodies in the lateral portion of the nucleus solitarius and in the caudal part of the spinal nucleus of the trigeminal nerve did not exhibit IR to either DBH or VIP. NPY-IR neurons in the area postrema occurred too infrequently for co-localization studies. The differential distribution of heterogeneous NPY-IR cell subpopulations may reflect the involvement of NPY in a variety of neuronal functions.Supported by the Deutsche Forschungsgemeinschaft, grant He 919/6-1     

牛生长激素结构不均一性的研究

本文报导用常规方法分离纯化的牛生长激素,在还原性SDS-11％聚丙烯酰胺凝胶电泳中呈分子量很接近的两条主带(22KD,21.5KD)。用单克隆抗体和多克隆抗体亲和层析技术分析了不同分子量形式的牛生长激素的转化,结果表明:牛生长激素可能在pH5.5条件下转化为21.5KD分子,在pH8.3条件下则转化18KD分子。这几种形式的牛生长激素均保留与抗体的结合力,但亲和力不尽相同,如在亲和层析的洗脱性质上存在差异。已检验分离并部分纯化了18KD分子以备作进一步的研究。     

倭竹属地理分布的研究

倭竹属Shibataea Makino隶于禾本科之竹亚科,现已知有8种,分布于我国东南部的浙江、福建、江苏、安徽、江西等省,广东、台湾两省有少量栽培,日本产1种。苏联、西德、印尼等国所栽培的倭竹均系自我国或日本引入。我国浙-闽地区产8种,且都有野生发现,是本属的现代分布中心。倭竹属植物体型矮小,常植于庭院或公园中供观赏。近年来盆景艺术迅速发展,微型园林日益兴起,倭竹属植物体态优美,常绿,耐寒且易于栽培,为广大园林工作者所垂青。