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101.
含单核中后期至后期花粉的蚕豆离体花药,经过2mM丁酸钠24小时预处理后,再漂浮培养于pH5.8或7.0的液体培养基。以不经预处理的作为对照。结果如下: 1.培养后9天内,丁酸钠预处理和培养基pH值并不明显影响花粉退化百分率。2.培养初期,pH7.0显著促进小孢子不等分裂。3.丁酸钠预处理抑制培养初期的小孢子有丝分裂,而后又显著增加小孢子均等分裂百分率。4.丁酸钠预处理导致小孢子有丝分裂类型的趋向改变。本文还对丁酸钠导致有丝分裂类型趋向改变的可能原因,进行了讨论。 相似文献
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104.
在不同生境的松林中,人工补充寄主卵都能提高寄生效果。但林地生境不同,寄生率有明显差异。在松阔混交林中补充寄主,其寄生率比对照提高5.5—16.2倍。植被稀疏的纯松林效果较差,补充的寄生率比对照提高3.0倍。 卵蜂种群消长随季节温度而变化,全年以5月中旬至6月下旬和9月中旬至10月中旬为两个寄生高峰。卵蜂种群与松毛虫种群的消长存在较明显的相依关系,卵蜂种群随着松毛虫种群的消长而消长。施药对卵蜂种群有较大影响,施药区比对照区的寄生率约降低一倍。在混交林中填充寄主卵,能促进卵蜂种群世代延续。在逐步改善林地生境的基础上,利用人工补充寄主,可以代替人工繁蜂放蜂。 相似文献
105.
Partial characterization of the plasma membrane ATPase from arho
0 petite strain ofSaccharomyces cerevisiae 总被引:2,自引:0,他引:2
James P. McDonough Patrick K. Jaynes Henry R. Mahler 《Journal of bioenergetics and biomembranes》1980,12(3-4):249-264
Crude membrane preparations of arho
0 mutant ofSaccharomyces cerevisiae exhibit Mg2+-dependent ATPase activity. Over the optimal pH range, 5.0–6.75, the apparentV
max of the enzyme equals 590 nmoles of ATP hydrolyzed per minute per milligram protein, with an apparentK
m
for ATP of 1.3 mM. ATP hydrolysis is insensitive to ouabain, venturicidin, aurovertin, and the protein inhibitor described by Pullman and Monroy; inhibited by oligomycin (at high concentrations) and sodium orthovandate, and it is sensitive to dicyclohexylcarbodiimide,p-hydroxymercuribenzoate, hydroxylamine, sodium fluoride, and sodium iodoacetate. The pH optimum and the inhibitor pattern distinguish the plasma membrane enzyme from the mitochondrial F1 ATPase still present in these cells (this activity is sensitive to efrapeptin, aurovertin, and the protein inhibitor, but resistant to DCCD). In addition, the activity of the plasma membrane enzyme and its affinity for ATP are responsive to changes in the composition of the growth medium, with the highest activity observed in cells grown on methyl--d-glucoside, a sugar which results not only in partial release from catabolite repression but also requires the induction of an active transport system for growth.Author to whom correspondence should be addressed; recipient of a Research Career Award No. K06 05060 from the Institute of General Medical Sciences. 相似文献
106.
宇宙空间是无限的,但适于人类生活和生物生存的生物圈则是有限的,它是个松散结构的大系统,其中包括许多类型的生物集团与无机环境结合而成的功能单元,即生态系统。在长期进化过程中,通过物质循环与能量交换,生物圈与生态系统之间建立了相互协调与补偿关系,并使生物圈及生态系统都具有一定限度的调节功能。人类对自然界的依存关系,以及自然界所形成的生态平衡是长期演化而形成的,人类亦参与和适应了此平衡过程。维持这种平衡的基础是有规律的物质循环和能量交換。此种质能流动则是通过 相似文献
107.
Summary An in situ thermocouple psychrometer was tested to determine its capability for tracking the changing leaf water potentials of aspen (Populus tremuloides Michx.) under controlled conditions and in the field. The results agreed with theoretical expectations — a sharp decline in leaf water potential during the photoperiods and recovery during the dark periods, each to progressively lower levels during a soil drying cycle. In the field, a mid-day pause in the declining leaf water potentials was noted and attributed to transient stomatal closure. Details of the construction and use of the assembly are given.The authors are plant physiologists, USDA Forest Service, Intermountain Forest and Range Experiment Station, Ogden, Utah 84401, stationed in Logan, Utah, at the Forestry Sciences Laboratory. 相似文献
108.
中国吻额蛛属一新种(蜘蛛目:皿蛛科:微蛛亚科) 总被引:1,自引:1,他引:0
本文描述了作者1986年采自湖北省神农架林区的吻额蛛属Aprifrontalia一新种:膨大吻额蛛,新种Aprifrontalia afflata sp.nov.。目前,除本新种外,世界仅报道过1种:Aprifrontalia mascula,本新种雄蛛最显著的特征是:其额向前突呈吻状,触肢胫节前端甚膨大。外雌器形成一斜向下方的突起。模式标本保存于白求恩医科大学生物教研室。 相似文献
109.
110.
While hormonal stimuli and mechanical stretch can induced cardiac-specific gene expression and in some cases cellular hypertrophy, the relationship between myocyte contraction frequency, gene expression, and myocyte growth has not been well characterized. In this study a new model system was developed in which cultures of neonatal rat ventricular myocytes were subjected to long term pacing of contractions with pulsatile electrical stimulation. Myocytes submitted to electrical stimulation for 3 days displayed dramatic increases in cellular size and myofibrillar organization, and a 5-10-fold increase in the expression of the cardiac genes atrial natriuretic factor and myosin light chain-2. Atrial natriuretic factor expression induced by electrical stimulation of contractions was inhibited by nifedipine or W7, indicating a dependence on calcium influx and calmodulin activity. Phosphoinositide hydrolysis and cAMP formation were not affected by electrical stimulation suggesting that gene induction occurred independently of the activation of protein kinases C or A above basal levels. These findings show that the cellular events associated with contraction, such as changes in cytoplasmic free calcium levels and/or cellular stretch, may serve as important determinants of myocyte growth and cardiac gene expression. 相似文献