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排序方式: 共有831条查询结果,搜索用时 31 毫秒
811.
Lan Yuan Yasuhiro Kuramitsu Yongqin Li Masanobu Kobayashi Masuo Hosokawa 《Cancer immunology, immunotherapy : CII》1995,41(6):355-362
We studied mechanisms of immunosuppression caused by tumor-derived transforming growth factor-ß (TGFß) and restoration of the immune response by treatment with bleomycin in rats bearing KDH-8 hepatoma. Interleukin-2 (IL-2) production from splenocytes of KDH-8-tumor-bearing rats progressively decreased as the KDH-8 tumor grew. IL-2 production from concanavalin-A-stimulated normal rat splenocytes was signficiantly inhibited by in vitro cultured KDH-8-tumor-cell-conditioned medium; this inhibition could be blocked by neutralizing the conditioned medium with anti-TGFß antibody. TGFß activities were found in KDH-8-tumor-tissue-conditioned medium without acid treatment and were found in tumor-cell-conditioned medium after acid treatment; TGFß mRNA and TGFß protein were found in cultured KDH-8 tumor cells. These results suggested that the KDH-8-tumor-derived TGFß might be involved in the inhibition of IL-2 production from splenocytes. To determine whether bleomycin chemotherapy could reduce tumor-derived TGFß and restore the immune responses, we treated KDH-8 tumor-bearing rats with bleomycin (5 mg/kg, one shot) at an appropriate time (before the occurrence of immunosuppression) resulting in a significiant reduction of TGFß activity in KDH-8 tumor tissues and restoration of IL-2 production from splenocytes of tumor-bearing rats; KDH-8 tumor growth ultimately regressed. In vitro experiments also showed that TGFß activity, mRNA expression, and protein synthesis in KDH-8 tumor cells were reduced by bleomycin treatment, and that bleomycin-treated-KDH-8-tumor-cell-conditioned medium did not inhibit IL-2 production from normal rat splenocytes. These results suggest that bleomycin treatment restored IL-2 production in tumor-bearing rats through reducing the tumor-derived TGFß. 相似文献
812.
Sato Yoshiki; Shimamoto Akira; Shobuike Takeo; Sugimoto Masanobu; Ikeda Hideo; Kuroda Shigetoshi; Furuichi Yasuhiro 《DNA research》1998,5(4):241-246
We isolatedand sequenced a human cDNA (designated as hSEP1)encoding both a homologue of mouse Dhm2 and budding yeast SEP1.The gene was shown to be locatedon the long arm of chromosome3 (3q25-26.1). The putative hSEP1 product (hSEP1p) consistedof 1694 amino acid residues with a molecular mass of about 190kDa. Northern blot analysis showeda major 10-kb mRNA expressedubiquitously in variousorgans as well as a minor 5.5-kb mRNAexpressed relatively highly in the testis and placenta. hSEP1pis localizedin the cytoplasm as examinedb y cytochemical andWestern blot analyses of fractionated cellular extracts. Thebiological function of hSEP1p was discussed in correlation withits cytoplasmic localization. 相似文献
813.
814.
815.
Masanobu Miyahara Eiji Okimasu Hiroaki Mikasa Shigeo Terada Hiroyuki Kodama Kozo Utsumi 《Archives of biochemistry and biophysics》1984,233(1):139-150
The mitochondrial dysfunction induced by anoxia in vitro was improved with chlorpromazine, cepharanthine, bromophenacyl bromide, and mepacrine without affecting phospholipid or adenine nucleotide metabolisms. The drugs inhibited lipid peroxidation by Fe2+, mitochondrial disruption by Ca2+, and membrane perturbation by lysolecithin, and retained the activity to control H+ permeability across mitochondrial membranes. The drugs appeared to preserve the functions by acting to suppress the development of membrane deterioration which may have resided in the deenergization of mitochondria in the absence of oxygen. 相似文献
816.
Masanobu Tara 《Journal of plant research》1979,92(2):151-156
In addition to various types of natural hybrids betweenAster ageratoides subsp.ovatus andKalimeris incisa reported earlier, a new backcross type has been discovered. This new type, characterized by the chromosomes of 2n=27L+54S,
was most probably produced through fertilization of a normally-reduced gamete of the F1 plant (2n=72=18L+54S) and an unreduced gamete of subsp.ovatus (2n=36=18L+18S). 相似文献
817.
818.
Masanobu Wada Shuichiro Inashima Takashi Yamada Satoshi Matsunaga 《Journal of applied physiology》2003,94(3):923-929
The effects of endurance training on the expression of myosin were electrophoretically analyzed in the deep portion of vastus lateralis muscle from the rat. A 10-wk running program led to increases (P < 0.01) in myosin heavy chain (MHC) 2a and 2d with a decrease (P < 0.01) in MHC(2b). Training also evoked a rearrangement of the isomyosin pattern with decreases in fast isomyosin (FM) 1 (P < 0.01) and FM2 (P < 0.05) and a rise in intermediate isomyosin (P < 0.01). These changes were accompanied by a 61% decrease (P < 0.01) in myosin light chain (MLC) 3F (11.8 +/- 2.7 vs. 4.6 +/- 4.2%). Two-dimensional electrophoresis made it possible to separate the triplet of isomyosins (FMb) consisting of MHC(2b). Training elicited a 26% decrease (P < 0.05) in the FM1b fraction within FMb, i.e., FM1b/(FM1b + FM2b + FM3b) (24.2 +/- 5.5 vs. 18.0 +/- 4.3%). These changes resulted in a 10% decrease (P < 0.05) in the MLC(3F) fraction, i.e., MLC(3F)/(MLC(1F) + MLC(3F)), in FMb (44.9 +/- 4.5 vs. 40.3 +/- 3.2%). These results suggest that endurance training may exert the depressive effect on the contractile velocity of type IIB fibers and that a training-induced decrease in the contractile velocity of whole muscle may be caused by alterations in fast alkali MLC complements within a given fiber type as well as by transitions in MHC-based fiber populations. 相似文献
819.
Masaki Ogata Takehiko Oomori Hiroyuki Soga Yuta Ota Ari Itoh Takaji Matsutani Masanobu Nanno Ryuji Suzuki Tsunetoshi Itoh 《Cell and tissue research》2009,335(2):371-382
In our earlier work, we found that, in mice, i.p. injection of anti-CD3 monoclonal antibody activated intraepithelial lymphocytes
(iIEL), leading to DNA fragmentation in villous epithelial cells of the duodenum and jejunum within 30 min. By 2 h after injection,
nearly half of the enterocytes had detached from the villi, and DNA fragmentation could barely be detected in the remaining
villous epithelium. We hypothesized that DNA had been repaired in enterocytes in which DNA fragmentation had previously been
induced. In this study, enterocytes became negative for TUNEL staining at 60 min after anti-CD3 treatment, prior to detachment.
The remaining villous epithelial cells, after DNA fragmentation and detachment, were found to be positive for 5-bromo-2-deoxyuridine
labeling. To confirm whether fragmented DNA had been repaired in situ, we investigated the appearance and/or mobilization
of DNA-repair-related proteins. Focus formation, a typical staining pattern of repair-related proteins including phosphorylated
H2AX, phospo-ATM substrate, and Nbs1, was observed 30 min after anti-CD3 injection, with the kinetics virtually identical
to that of DNA fragmentation. The co-localization of γ-H2AX and phospo-ATM substrate was also confirmed. The disappearance
of a positive reaction for TUNEL staining in previously fragmented DNA, the appearance of representative DNA-repair-related
proteins, the coincidence of the kinetics of DNA fragmentation and this appearance of DNA-repair-related proteins, and the
co-localization of two of the repair-related proteins strongly indicated that enterocyte DNA could be repaired after it had
been fragmented in vivo. Thus, DNA fragmentation per se may not necessarily be an immediate sign of cell death.
This work was supported in part by a Grant-in-aid for Scientific Research from the Ministry of Education, Science and Culture,
Japan (16590132 to T.M., 16390045 to T.I., and 20590181 to M.O.). 相似文献
820.
Not‐self or denatured nucleic acids are recognized by pattern recognition receptors localized mainly in endosomes and cytoplasm, such as Toll‐like receptor (TLR) 3, TLR7, TLR9, retinoic acid‐inducible gene‐I, DNA‐dependent activator of IFN‐regulatory factors and other receptors. The binding of polyriboinosinic:polyribocytidylic acid (poly I:C), a synthetic dsRNA that robustly induces type I interferon, to a putative cell‐surface receptor on a rabbit kidney cell line, RK13, has been analyzed by the authors and RK13 cells found to capture poly I:C in a specific fashion with sufficient affinity. These findings suggest that an alternative receptor to poly I:C participates in the induction of type 1 interferon, which localizes on cell surfaces. Although the nature of this molecule has not yet been identified, accumulating evidence has led the present authors to speculate that there are undefined classes of RNA‐recognition molecules on cell surfaces and that these are unlikely to be categorized as previously reported dsRNA receptors. Although many years have passed since this possibility was first reported by the present authors, it remains attractive. In this article, previously reported cell‐surface dsRNA receptors are reviewed in comparison with other receptors reported to date that are firmly involved in the innate immune‐sensing of nucleic acids. 相似文献