Mutagenicity of bisphenol A and its suppression by interferon-α in human RSa cells

Bisphenol A is used as a monomer in the production of polycarbonate plastic products. The widespread use of bisphenol A has raised concerns about its effects in humans. Since there is little information on the mutagenic potential of the chemical, the mutagenicity of bisphenol A was tested using human RSa cells, which has been utilized for identification of novel mutagens. In genomic DNA from cells treated with bisphenol A at concentrations ranging from 1×10⁻⁷ to 1×10⁻⁵ M, base substitution mutations at K-ras codon 12 were detected using PCR and differential dot-blot hybridization with mutant probes. Mutations were also detected using the method of peptide nucleic acid (PNA)-mediated PCR clamping. The latter method enabled us to detect the mutation in bisphenol A-treated cells at a dose (1×10⁻⁸ M) equivalent to that typically found in the environment. Induction of ouabain-resistant (Oua^R) phenotypic mutation was also found in cells treated with 1×10⁻⁷ and 1×10⁻⁵ M of bisphenol A. The induction of K-ras codon 12 mutations and Oua^R mutations was suppressed by pretreating RSa cells with human interferon (HuIFN)-α prior to bisphenol A treatment. The cells treated with bisphenol A at the concentration of 1×10⁻⁶ M elicited unscheduled DNA synthesis (UDS). These findings suggested that bisphenol A has mutagenicity in RSa cells as well as mutagens that have been tested in these cells, and furthermore, that a combination of the PNA-mediated PCR clamping method with the human RSa cell line may be used as an assay system for screening the mutagenic chemicals at very low doses.     

Mineral resources in life cycle impact assessment—part I: a critical review of existing methods

Purpose

The safeguard subject of the Area of Protection “natural Resources,” particularly regarding mineral resources, has long been debated. Consequently, a variety of life cycle impact assessment methods based on different concepts are available. The Life Cycle Initiative, hosted by the UN Environment, established an expert task force on “Mineral Resources” to review existing methods (this article) and provide guidance for application-dependent use of the methods and recommendations for further methodological development (Berger et al. in Int J Life Cycle Assess, 2020).

Methods

Starting in 2017, the task force developed a white paper, which served as its main input to a SETAC Pellston Workshop® in June 2018, in which a sub-group of the task force members developed recommendations for assessing impacts of mineral resource use in LCA. This article, based mainly on the white paper and pre-workshop discussions, presents a thorough review of 27 different life cycle impact assessment methods for mineral resource use in the “natural resources” area of protection. The methods are categorized according to their basic impact mechanisms, described and compared, and assessed against a comprehensive set of criteria.

Results and discussion

Four method categories have been identified and their underlying concepts are described based on existing literature: depletion methods, future efforts methods, thermodynamic accounting methods, and supply risk methods. While we consider depletion and future efforts methods more “traditional” life cycle impact assessment methods, thermodynamic accounting and supply risk methods are rather providing complementary information. Within each method category, differences between methods are discussed in detail, which allows for further sub-categorization and better understanding of what the methods actually assess.

Conclusions

We provide a thorough review of existing life cycle impact assessment methods addressing impacts of mineral resource use, covering a broad overview of basic impact mechanisms to a detailed discussion of method-specific modeling. This supports a better understanding of what the methods actually assess and highlights their strengths and limitations. Building on these insights, Berger et al. (Int J Life Cycle Assess, 2020) provide recommendations for application-dependent use of the methods, along with recommendations for further methodological development.

     

Soil moisture variability on a steep slope near a ridge in a forested mountain range, Shikoku, Japan: a model study

Variability in soil moisture on a steep slope near a ridge in a forested mountain range, Shikoku, Japan, was studied observationally and numerically. Vertically integrated soil moisture, from a depth of ?60 cm to the surface, W, was introduced as a key indicator, and its seasonal variation was analysed on a daily basis from August 2011 to August 2012. The “bucket with a bottom hole” (BBH) model of Teshima et al. (2006) was improved to consider the forest environment in simulating the variation in W. A “big-leaf” model was incorporated into the modified BBH model to estimate transpiration and interception by trees. The simulated soil moisture agreed reasonably with observed values on a daily to inter-seasonal timescale.     

Studies on the behavior of meiotic protoplasts IV. Protoplasts isolated from microsporocytes of liliaceous plants

Enzymatic isolation of protoplasts from microsporocytes of various species of liliaceous plants is described along with some of the features of the isolated meiotic protoplasts. Protoplasts are produced with a high viability from coherent filaments of cells preculture for 24 hr during premeiosis and early meiotic prophase, but with low survival rates from free cells at late prophase. The suspensions of protoplasts contain multinucleate cells produced by spontaneous fusion at various frequencies up to 40%. In the enzyme solution meiotic protoplasts adhere to one another. When isolated at meiotic prophase, protoplasts may be cultured through the meiotic cycle.     

Assessing the Replacement of Electrical Home Appliances for the Environment

To evaluate whether replacing an existing product with a new, more energy‐efficient product is environmentally preferable, we used an assessment approach based on life cycle assessment. With this approach, consumers can assess various replacement products, including products of different sizes or environmental performance in addition to consideration of various conditions of product use. The approach utilizes a diagram in which replacement conditions of products are compared with iso‐environmental‐load lines to determine the appropriateness of replacement. The approach also allows the assessment of energy and resource consumption and environmental impacts not only during the use stage, but also at other product stages. Iso‐environmental‐load lines to assess delayed replacement were also examined and derived. We then applied the approach in a case study of energy consumption by replacing three types of electric home appliances in Japan: TVs, air conditioners, and refrigerators. The results of assessment showed that replacing refrigerators after 8–10 years of use was preferable even if the replacement product was larger. The appropriateness of replacing TVs and air conditioners based on energy consumption depended on the replacement product and on the duration of daily use, and in several cases, delayed replacement was preferable. Replacement of air conditioners after 8–10 years of use was not preferable if the consumer already owned the most energy‐efficient product at the time of the purchase. The necessity of accounting for a variety of available replacement products was confirmed.