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91.
Xiaojun Cheng Meiyao Pan Zhiguo E Yong Zhou Baixiao Niu Chen Chen 《The Plant journal : for cell and molecular biology》2020,104(1):124-137
Fertilization Independent Endosperm (FIE) is an essential member of Polycomb Repressive Complex 2 (PRC2) that plays important roles in the developmental regulation of plants. OsFIE1 and OsFIE2 are two FIE homologs in the rice genome. Here, we showed that OsFIE1 probably duplicated from OsFIE2 after the origin of the tribe Oryzeae, but has a specific expression pattern and methylation landscape. During evolution, OsFIE1 underwent a less intensive purifying selection than did OsFIE2. The mutant osfie1 produced smaller seeds and displayed reduced dormancy, indicating that OsFIE1 predominantly functions in late seed development. Ectopic expression of OsFIE1, but not OsFIE2, was deleterious to vegetative growth in a dose‐dependent manner. The newly evolved N‐terminal tail of OsFIE1 was probably not the cause of the adverse effects on vegetative growth. The CRISPR/Cas9‐derived mutant osfie2 exhibited impaired cellularization of the endosperm, which suggested that OsFIE2 is indispensable for early seed development as a positive regulator of cellularization. Autonomous endosperm was observed in both OsFIE2+? and osfie1/OsFIE2+? but at a very low frequency. Although OsFIE1‐PRC2 exhibited H3K27me3 methyltransferase ability in plants, OsFIE1‐PRC2 is likely to be less important for development in rice than is OsFIE2‐PRC2. Our findings revealed the functional divergence of OsFIE1 and OsFIE2 and shed light on their distinct evolution following duplication. 相似文献
92.
法尼醇(Farnesol,FOH)是由焦磷酸异戊烯基(IPP)和焦磷酸二甲基烯丙基(DMAPP)合成的法尼酰基焦磷酸盐(FPP)去焦磷酸化作用生成的。在类球红细菌中IPP和DMAPP是由MEP途径生成,而完整的MEP途径需要消耗大量的辅因子NADPH,增加胞内NADPH的量有可能强化FOH的合成。文中从增加NADPH的生成和降低NADPH的消耗这两个策略出发,分别干扰了编码6-磷酸葡萄糖异构酶基因(pgi)和谷氨酸脱氢酶基因(gdhA)的表达,同时强化了磷酸戊糖途径中6-葡萄糖磷酸脱氢酶基因(zwf)和6-葡萄糖酸磷酸脱氢酶基因(gnd)的表达。实验结果表明,经改造的菌株NADPH含量显著增加,干扰菌株中菌株RSpgii的产量较高,为3.91 mg/g,在过表达的菌株中同时过表达zwf和gnd基因的重组菌株(RSzg)的FOH产量提高到了3.43 mg/g。为了获得FOH产量更高的菌株,以RSpgii为出发菌株,分别与zwf和gnd组合调控,获得的菌株RSzgpi的产量达到了最高量为4.48 mg/g,是出发菌株RS-GY2产率的2.24倍。 相似文献
93.
黄土区土质与土石质塿土堆积体水力侵蚀过程差异 总被引:2,自引:0,他引:2
利用室内模拟降雨试验,研究了不同雨强及坡度条件下黄土区土质(不含砾石)与土石质(砾石质量分数30%)塿土堆积体的水动力学特征、侵蚀特征及侵蚀动力机制的差异。结果表明: 砾石存在改变了堆积体坡面的水动力学特性,与土质坡面相比,土石质坡面的流速、弗汝德数、单位径流功率和过水断面单位能分别减少1.7%~49.7%、6.7%~60.6%、2.0%~44.6%和1.0%~26.7%;曼宁糙率系数、径流剪切力分别增加6.2%~169.4%、5.7%~79.3%。2.0、2.5 mm·min-1雨强下,土石质坡面侵蚀速率较土质坡面降低26.2%~89.9%,砾石的减沙效益显著。2种堆积体的侵蚀速率与水动力学参数间均可用线性函数拟合,与土质坡面相比,土石质坡面的可蚀性参数均降低,降幅为56.1%~73.3%;而临界水动力学参数中径流剪切力增加11.1%,径流功率、单位径流功率和过水断面单位能分别减少25.4%、64.0%和5.0%。砾石的存在一定程度上控制了工程堆积体坡面降雨侵蚀过程。 相似文献
94.
土壤线虫是地下食物网的重要组成部分, 在生态系统能量流动和物质循环中起着至关重要的作用。大量研究报道了肥力等土壤环境对土壤线虫物种多样性及各功能群多度的影响, 而我们对土壤线虫功能多样性如何响应土壤环境变化依然知之甚少。本研究以群落水平个体大小和个体大小多样性表征土壤线虫功能多样性。在青藏高原高寒草甸选择3个研究点, 调查和分析了不同生境(沟底平地、阴坡、阳坡和山顶)土壤线虫物种多样性、各功能群多度和功能多样性及其与土壤理化因子和植物多样性的关系。结果表明: (1)土壤线虫个体多度和物种多样性在阳坡最高, 随土壤pH值和土壤总磷增加而升高; 而基于个体大小的土壤线虫功能多样性主要受土壤养分影响, 随土壤总氮和有机质增加而增加, 随土壤总磷含量增加而减少; (2)食细菌和食真菌线虫多度在沟底最高, 植食与捕食杂食线虫多度在山顶最低; 除捕食杂食线虫外, 各功能群多度也主要随土壤磷增加而升高; 除食真菌线虫外, 各功能群多度随植物物种丰富度的增加而减少。本研究强调了土壤线虫物种和功能多样性受不同土壤环境因子的影响, 丰富了土壤线虫多样性研究的内容, 为理解高寒草甸土壤动物多样性形成、维持和变化提供了更广阔的 视角。 相似文献
95.
Yang Xiangdong Yang Jing Li Haiyun Niu Lu Xing Guojie Zhang Yuanyu Xu Wenjing Zhao Qianqian Li Qiyun Dong Yingshan 《Transgenic research》2020,29(2):187-198
Transgenic Research - Pathogenic fungi represent one of the major biotic stresses for soybean production across the world. Sclerotinia sclerotiorum, the causal agent of Sclerotinia stem rot, is a... 相似文献
96.
为了研究软枣猕猴桃授粉规律,以11年生软枣猕猴桃紫果3号为试验材料,设置剪留花柱数量为0、2、5、8、11、14、17和23(全留对照组)共8个处理。人工授粉,收获后调查测定其单果重、坐果率、果型指数、果实可溶性固形物含量和果实内含种子数量。结果表明:随着授粉柱头数的增加,单果重等主要指标相应增加;当授粉柱头数增至为8时,其果型指数和果实可溶性固形物含量与对照全留柱头23相比差异不明显;当授粉柱头数增至为11时,其单果重、坐果率和果实内含种子数量与对照全留柱头23相比差异不明显。据此软枣猕猴桃充分授粉的数量级指标为11,当授粉柱头数小于11时,产量降低、品质下降;当授粉柱头大于11时,浪费花粉。生产上可利用猕猴桃精准充分授粉技术,节约使用花粉或减少果园雄株数量,以提高生产效率。 相似文献
97.
Fajuan Cheng Bin Zheng Jianwei Wang Guiting Zhao Zhongshun Yao Zhihong Niu Wei He 《Bioscience reports》2021,41(6)
Histone deacetylase (HDAC) 10, a class II family, has been implicated in various tumors and non-tumor diseases, which makes the discovery of biological functions and novel inhibitors a fundamental endeavor. In cancers, HDAC10 plays crucial roles in regulating various cellular processes through its epigenetic functions or targeting some decisive molecular or signaling pathways. It also has potential clinical utility for targeting tumors and non-tumor diseases, such as renal cell carcinoma, prostate cancer, immunoglobulin A nephropathy (IgAN), intracerebral hemorrhage, human immunodeficiency virus (HIV) infection and schizophrenia. To date, relatively few studies have investigated HDAC10-specific inhibitors. Therefore, it is important to study the biological functions of HDAC10 for the future development of specific HDAC10 inhibitors. In this review, we analyzed the biological functions, mechanisms and inhibitors of HDAC10, which makes HDAC10 an appealing therapeutic target. 相似文献
98.
Kai Che Wenkai Han Danxia Li Shuxia Cui Mingxin Zhang Xiaokun Yang Haitao Niu 《Bioscience reports》2021,41(2)
Background: Glycolysis was a representative hallmark in the tumor microenvironment (TME), and we aimed to explore the correlations between glycolysis with immune activity and clinical traits in bladder urothelial carcinoma (BLCA).Methods: Our study obtained glycolysis scores for each BLCA samples from TCGA by a single-sample gene set enrichment analysis (ssGSEA) algorithm, based on a glycolytic gene set. The relationship between glycolysis with prognosis, clinical characteristics, and immune function were investigated subsequently.Results: We found that enhanced glycolysis was associated with poor prognosis and metastasis in BLCA. Moreover, glycolysis had a close correlation with immune function, and enhanced glycolysis increased immune activities. In other words, glycolysis had a positive correlation with immune activities. Immune checkpoints such as IDO1, CD274, were up-regulated in high-glycolysis group as well.Conclusion: We speculated that in BLCA, elevated glycolysis enhanced immune function, which caused tumor cells to overexpress immune checkpoints to evade immune surveillance. Inhibition of glycolysis might be a promising assistant for immunotherapy in bladder cancer. 相似文献
99.
Su-Fang Niu Yun Zhai Ren-Xie Wu Zhen-Bang Liang Hao-Ran Zhang Zhong-Lu Li Qi Qiu Ling-Li Zhou 《Zeitschrift fur angewandte Ichthyologie》2021,37(2):308-313
Trachurus japonicus is an economically important fish in the northwestern Pacific Ocean. However, its resources have declined seriously and there is an urgent need for a wide-range of investigations of the existing genetic resources. This requires a large number of diverse molecular markers with high discriminating power. In this study, we identified 43,264 perfect SSRs in T. japonicus genome using SLAF-seq technology. Of these, we randomly selected 106 SSRs (tri-nucleotide to hexa-nucleotide) to test for polymorphism. Eventually, we successfully developed a total of 33 loci including 8 tri-nucleotide and 25 long repeat motifs (tetra-nucleotide to hexa-nucleotide). The number of alleles (Na) of these loci ranged from 4 to 24 (mean 12.6). The observed heterozygosity (Ho) and expected heterozygosity (He) varied from 0.258 to 0.969 (mean 0.723) and from 0.452 to 0.962 (mean 0.827), respectively. All loci except TJ6-7 were highly informative (PIC > 0.5). These results showed that the shortlisted 33 loci exhibited moderate to relatively high genetic diversity, of which 18 were regarded as highly polymorphic and well-resolved. In summary, these diverse and potential microsatellites detected in our study provide substantial genetic basis for the screening of polymorphic SSR markers of T. japonicus and also provide a powerful tool to perform further studies on the genetic resource assessment and conservation of T. japonicus. 相似文献
100.
Zhou Rongmiao Li Yan Wang Na Niu Chaoxu Huang Xi Cao Shiru Huo Xiangran 《Molecular biology reports》2021,48(2):1485-1491
Molecular Biology Reports - DNA repair system plays a crucial role in maintaining genomic integrity and stability and in protecting against cancer. Poly(ADP-ribose) polymerase 1 (PARP1) functions... 相似文献