全文获取类型
收费全文 | 71篇 |
免费 | 3篇 |
出版年
2015年 | 1篇 |
2011年 | 1篇 |
2010年 | 1篇 |
2009年 | 3篇 |
2008年 | 1篇 |
2007年 | 2篇 |
2006年 | 4篇 |
2005年 | 1篇 |
2003年 | 2篇 |
2002年 | 1篇 |
2001年 | 1篇 |
2000年 | 2篇 |
1999年 | 1篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1982年 | 2篇 |
1981年 | 2篇 |
1980年 | 3篇 |
1979年 | 7篇 |
1978年 | 1篇 |
1976年 | 3篇 |
1974年 | 1篇 |
1973年 | 3篇 |
1972年 | 3篇 |
1971年 | 5篇 |
1970年 | 1篇 |
1969年 | 2篇 |
1968年 | 2篇 |
1967年 | 3篇 |
1966年 | 5篇 |
1965年 | 2篇 |
排序方式: 共有74条查询结果,搜索用时 31 毫秒
41.
Irina E Kovaleva Lyudmila A Novikova Pavel A Nazarov Sergei I Grivennikov Valentin N Luzikov 《European journal of biochemistry》2003,270(2):222-229
Topogenesis of cytochrome p450scc, a resident protein of the inner membrane of adrenocortical mitochondria, is still obscure. In particular, little is known about the cause of its tissue specificity. In an attempt to clarify this point, we examined the process in Saccharomyces cerevisiae cells synthesizing cytochrome p450scc as its native precursor (pCYP11A1) or versions in which its N-terminal addressing presequence had been replaced with those of yeast mitochondrial proteins: CoxIV(1-25) and Su9(1-112). We found the pCYP11A1 and CoxIV(1-25)-mCYP11A1 versions to be effectively imported into yeast mitochondria and subjected to proteolytic processing. However, only minor portions of the imported proteins were incorporated into mitochondrial membranes, whereas their bulk accumulated as aggregates insoluble in 1% Triton X-100. Along with previously published data, this suggests that a distinguishing feature of the import of the CYP11A1 precursors into yeast mitochondria is their easy translocation into the matrix where the foreign proteins mainly undergo proteolysis or aggregation. The fraction of CYP11A1 that happens to be inserted into the inner mitochondrial membrane is effectively converted into the catalytically active holoenzyme. Experiments with the Su9(1-112)-mCYP11A1 construct bearing a re-export signal revealed that, after translocation of the fused protein into the matrix and its processing, the Su9(67-112) segment ensures association of the mCYP11A1 body with the inner membrane, but proper folding of the latter does not take place. Thus it can be said that the most specific stage of CYP11A1 topogenesis in adrenocortical mitochondria is its confinement and folding in the inner mitochondrial membrane. In yeast mitochondria, only an insignificant portion of the imported CYP11A1 follows this mechanism. 相似文献
42.
43.
44.
L. A. Novikova Ya. V. Faletrov I. E. Kovaleva S. Mauersberger V. N. Luzikov V. M. Shkumatov 《Biochemistry. Biokhimii?a》2009,74(13):1482-1504
This review summarizes data about structural and functional organization of steroidogenic P450-dependent enzymatic systems.
Problems of catalysis of steroid substrate transformation, special features of mitochondrial type P450scc topogenesis, and
abilities of some microbial electron transport proteins to support P450 activity in vitro and in vivo are considered. Principal steps in the creation and catalytic properties of transgenic strains of Escherichia coli, Saccharomyces cerevisiae, and Yarrowia lipolytica expressing both mammalian steroidogenic P450s and the corresponding electron transport proteins are also described. Achievements
and prospects of using such transgenic strains for biotechnological synthesis and pharmacological screening are considered. 相似文献
45.
The complete amino-acid sequences of two isocytochromes c (larval- and adult-type cytochromes c) purified from the housefly Musca domestica L. were determined. Their sequences differed at six positions from each other. More than 90% of the total cytochrome c was larval-type during larval stages. The amount of adult-type cytochrome c increased rapidly from 1 day before adult emergence, making the total cytochrome c content increase to approximately 2.5-times as much cytochrome c content as in larvae. Although the major cytochrome c species in flight muscles was adult-type, imaginal disks contained mainly larval-type cytochrome c. 相似文献
46.
47.
Multiplicity of mitochondrial proteinases in yeast 总被引:2,自引:0,他引:2
48.
Shchekotikhin AE Dezhenkova LG Susova OY Glazunova VA Luzikov YN Sinkevich YB Buyanov VN Shtil AA Preobrazhenskaya MN 《Bioorganic & medicinal chemistry》2007,15(7):2651-2659
The efficacy of anthracycline based anticancer drugs is limited by pleiotropic drug resistance of tumor cells. Aiming at the design of anthracyclinone congeners capable of circumventing drug resistance, we synthesized naphthoindole containing derivatives of tryptophan and tryptamine. In doing so we adapted the traditional, gramine based approach for tryptophan and tryptamine synthesis. The most potent new compound, 3-(2-aminoethyl)-4,11-dihydroxynaphtho[2,3-f]indole-5,10-dione (16), was equally cytotoxic (IC(50) within low micromolar concentrations) for human K562 leukemia and HCT116 colon carcinoma cell lines and their isogenic sublines with genetically defined determinants of altered drug response, that is, the expression of the multidrug transporter P-glycoprotein and loss of pro-apoptotic p53. Each of these mechanisms conferred resistance to the reference drug adriamycin. In contrast, naphthotryptamine 16, although less potent than adriamycin, was equally toxic for wild type cell lines and drug resistant counterparts. Moreover, at 3-5 microM 16 inhibited topoisomerase I in vitro. Thus, our novel naphthoindole based derivative of tryptamine gained new activities important for anticancer therapy, namely, suppression of topoisomerase I and the ability to overcome resistance mediated by P-glycoprotein expression and p53 dysfunction. 相似文献
49.
The reconstitution of NADH oxidase, succinate oxidase and the complete respiratory chain from NADH: cytochrome c oxidoreductase, succinate: coenzyme Q oxidoreductase, cytochrome oxidase and cytochrome c was studied under various conditions. The formation of these multi-enzyme systems was prevented by cobra venom phospholipase. Reconstitution was possible in the presence of cobra venom only if the medium contained NADH (or succinate) and O2. Bovine serum albumin prevented the formation of NADH oxidase at low temperatures but hardly affected this process at 38–42°. It also increased the thermal stability of the reconstituted system. Reconstitution of NADH oxidase did not occur in the presence of potassium oleate, and bovine serum albumin completely eliminated the effect of the latter. However, bovine serum albumin did not protect the respiratory chain from the action of phospholipase. Therefore, the presence of NADH was necessary for the reconstitution of NADH oxidase at 38° in a medium containing bovine serum albumin and cobra venom.
Thus, the natural agents indicated above have a substantial effect on the reconstitution of the respiratory chain. Reconstitution becomes possible with a strictly definite ratio between the effects of different external factors. A special part in the formation of the respiratory chain is played by substrates having a specific influence on its structure. 相似文献
50.
V. N. Luzikov A. S. Zubatov E. I. Rainina 《Journal of bioenergetics and biomembranes》1973,5(3):129-149
Summary Prolonged deaeration ofSaccharomyces cerevisiae cells results in degenerative changes in mitochondria which can be revealed when measuring the enzymic activities of the respiratory chain in isolated organelles and by electron microscope examination of the cells. The same changes are observed after a 3-h incubation of the cells with cyanide or carbonyl cyanide, m-chlorophenyl hydrazone in aerobic conditions. These results suggest the important role of oxidative phosphorylation in the maintenance of the integrity of mitochondria in the cell.The sensitivity of yeast mitochondria to anaerobiosis and cyanide changes as the culture grows. Mitochondria are especially labile during the early exponential growth phase when their respiratory system and structure are not fully formed. Possible reasons for and the mechanism of degradation of mitochondriain vivo are discussed. 相似文献