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991.
为探讨高脂食物对小型哺乳动物能量代谢的影响及其与基础代谢率(Basal metabolic rate, BMR)的关系,将成年雌性黑线仓鼠(Cricetulus barabensis)分为高、低BMR组,每组再随机分为低脂、高脂食物组,驯化6周后,测定体重、摄入能和代谢率,以及消化酶活力、褐色脂肪组织(Brown adipose tissue, BAT)和主要内脏器官与肌肉的细胞色素c氧化酶(Cytochrome c oxidase, COX)活性、解偶联蛋白(Uncoupling protein, UCP) mRNA表达等。结果显示,高脂食物对高、低BMR组动物体重均无显著影响。与低脂食物组相比,高脂食物组的摄食量、摄入能和消化能显著下降,小肠脂肪酶活力显著增强,消化率明显增加,但高、低BMR组的组间差异不显著。夜间代谢水平显著高于昼间,高脂食物使高BMR组的夜间代谢率显著升高。BAT、肌肉和内脏器官COX活性不受高脂食物的影响,高、低BMR组的组间差异也不显著。高脂食物组仅肝脏UCP2表达显著上调。结果表明,能量摄入和消化系统形态及功能的可塑性调节是黑线仓鼠应对高脂食物的主要策略;黑线仓鼠的代谢率具有显著的昼夜节律,既受高脂食物的影响,也与动物自身的BMR水平有关,但UCP表达具有组织特异性,这可能不是导致BMR个体差异的因素。 相似文献
992.
993.
为探讨东亚-澳大利西亚迁徙路线上的鸻鹬类体内有机氯农药的含量及来源,本研究以该迁徙路线上的大滨鹬(Calidris tenuirostris)和红腹滨鹬(C.canutus)为研究对象,用索氏提取法对这两种鸟的胸肌和皮下脂肪中的有机污染物进行萃取,并用气相色谱法对19种有机氯农药进行检测。结果表明,HCHs、DDTs、硫丹Ⅱ等14种有机氯农药在大滨鹬和红腹滨鹬的组织中均有不同程度的检出,所有样品中的含量最高值达1 573.5 ng/g脂重;在检出的14种有机氯农药中,α-HCH、β-HCH、γ-HCH、p,p′-DDE、硫丹Ⅱ、硫丹硫酸酯和/或p,p′-DDT的检出率达100%;在大滨鹬的肌肉组织、红腹滨鹬的肌肉和脂肪组织中,p,p′-DDE的残留量最高;而在大滨鹬的脂肪组织中硫丹硫酸酯和/或p,p′-DDT的含量最高;目标物中的艾氏剂、异狄氏剂、七氯、反式氯丹等未达检出限或含量较低。我们对比了不同物种及不同组织样本中有机氯农药的富集程度,红腹滨鹬的肌肉组织中HCHs的沉积量显著高于大滨鹬,而大滨鹬的脂肪组织中硫丹硫酸酯和/或p,p′-DDT的含量显著高于红腹滨鹬。此外,分别对比两个物种的肌肉组织和脂肪组织中有机氯农药沉积量,部分有机氯农药在脂肪组织中的沉积量显著高于肌肉组织,说明相比于肌肉组织,有机氯农药可能更易于在脂肪组织中累积。 相似文献
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995.
Qi Zhang Pu Wang Haoli Hou Hao Zhang Junjun Tan Yan Huang Yingnan Li Jinping Wu Zhengming Qiu Lijia Li 《Protoplasma》2017,254(1):167-179
Histone acetylation plays a critical role in controlling chromatin structure, and reactive oxygen species (ROS) are involved in cell cycle progression. To study the relationship between histone acetylation and cell cycle progression in plants, sodium butyrate (NaB), a histone deacetylase (HDAC) inhibitor that can cause a significant increase in histone acetylation in both mammal and plant genomes, was applied to treat maize seedlings. The results showed that NaB had significant inhibition effects on different root zones at the tissue level and caused cell cycle arrest at preprophase in the root meristem zones. This effect was accompanied by a dramatic increase in the total level of acetylated lysine 9 on histone H3 (H3K9ac) and acetylated lysine 5 on histone H4 (H4K5ac). The exposure of maize roots in NaB led to a continuous rise of intracellular ROS concentration, accompanied by a higher electrolyte leakage ratio and malondialdehyde (MDA) relative value. The NaB-treated group displayed negative results in both TdT-mediated dUTP nick end labelling (TUNEL) and γ-H2AX immunostaining assays. The expression of topoisomerase genes was reduced after treatment with NaB. These results suggested that NaB increased the levels of H3K9ac and H4K5ac and could cause preprophase arrest accompanied with ROS formation leading to the inhibition of DNA topoisomerase. 相似文献
996.
997.
Bo Xiong Shuang Ye Xia Qiu Ling Liao Guochao Sun Jinyu Luo Lin Dai Yi Rong Zhihui Wang 《Acta Physiologiae Plantarum》2017,39(4):98
The role of exogenous spermidine (Spd) in alleviating fruit granulation in the grafted seedlings of a Citrus cultivar (Huangguogan) was investigated. Granulation resulted in increased electrical conductivity, cell membrane permeability, and total pectin, soluble pectin, cellulose, and lignin contents. However, it decreased the activities of superoxide dismutase, peroxidase, and catalase, as well as the (Spd + Spm):Put ratio. The application of exogenous Spd onto Huangguogan seedlings significantly increased proline and ascorbate contents, but decreased the H2O2 and O 2 ?· levels, which suggested that exogenous Spd provided some protection from oxidative damage. In addition, exogenous Spd decreased cell membrane permeability and MDA content, and increased the (Spd + Spm):Put ratio. The activities of antioxidant enzymes, such as catalase, peroxidase, and superoxide dismutase, were increased in Spd-treated seedlings affected by fruit granulation, resulting in a decrease in oxidative stress levels. The protective effects of Spd were reflected by a decrease in superoxide levels through osmoregulation, increased proline and ascorbate contents, and increased antioxidant activities. Our observations reveal the importance of exogenous Spd in alleviating citrus fruit granulation. 相似文献
998.
Hippo kinases maintain polarity during directional cell migration in Caenorhabditis elegans 下载免费PDF全文
Guoxin Feng Zhiwen Zhu Wen‐Jun Li Qirong Lin Yongping Chai Meng‐Qiu Dong Guangshuo Ou 《The EMBO journal》2017,36(3):334-345
Precise positioning of cells is crucial for metazoan development. Despite immense progress in the elucidation of the attractive cues of cell migration, the repulsive mechanisms that prevent the formation of secondary leading edges remain less investigated. Here, we demonstrate that Caenorhabditis elegans Hippo kinases promote cell migration along the anterior–posterior body axis via the inhibition of dorsal–ventral (DV) migration. Ectopic DV polarization was also demonstrated in gain‐of‐function mutant animals for C. elegans RhoG MIG‐2. We identified serine 139 of MIG‐2 as a novel conserved Hippo kinase phosphorylation site and demonstrated that purified Hippo kinases directly phosphorylate MIG‐2S139. Live imaging analysis of genome‐edited animals indicates that MIG‐2S139 phosphorylation impedes actin assembly in migrating cells. Intriguingly, Hippo kinases are excluded from the leading edge in wild‐type cells, while MIG‐2 loss induces uniform distribution of Hippo kinases. We provide evidence that Hippo kinases inhibit RhoG activity locally and are in turn restricted to the cell body by RhoG‐mediated polarization. Therefore, we propose that the Hippo–RhoG feedback regulation maintains cell polarity during directional cell motility. 相似文献
999.
1000.