全文获取类型
收费全文 | 7484篇 |
免费 | 1014篇 |
国内免费 | 1篇 |
出版年
2021年 | 93篇 |
2016年 | 118篇 |
2015年 | 196篇 |
2014年 | 211篇 |
2013年 | 300篇 |
2012年 | 348篇 |
2011年 | 349篇 |
2010年 | 217篇 |
2009年 | 222篇 |
2008年 | 318篇 |
2007年 | 308篇 |
2006年 | 281篇 |
2005年 | 242篇 |
2004年 | 258篇 |
2003年 | 249篇 |
2002年 | 269篇 |
2001年 | 232篇 |
2000年 | 231篇 |
1999年 | 193篇 |
1998年 | 78篇 |
1997年 | 87篇 |
1996年 | 81篇 |
1995年 | 63篇 |
1994年 | 72篇 |
1993年 | 67篇 |
1992年 | 130篇 |
1991年 | 136篇 |
1990年 | 152篇 |
1989年 | 134篇 |
1988年 | 137篇 |
1987年 | 120篇 |
1986年 | 126篇 |
1985年 | 141篇 |
1984年 | 108篇 |
1983年 | 124篇 |
1982年 | 100篇 |
1981年 | 85篇 |
1980年 | 83篇 |
1979年 | 113篇 |
1978年 | 87篇 |
1977年 | 78篇 |
1976年 | 71篇 |
1975年 | 87篇 |
1974年 | 85篇 |
1973年 | 83篇 |
1972年 | 84篇 |
1971年 | 66篇 |
1970年 | 88篇 |
1969年 | 77篇 |
1967年 | 71篇 |
排序方式: 共有8499条查询结果,搜索用时 343 毫秒
991.
The structures, electron configurations, magnetic susceptibilities, spectroscopic properties, molecular orbital energies and spin density distributions, redox properties and reactivities of iron corrolates having chloride, phenyl, pyridine, NO and other ligands are reviewed. It is shown that with one very strong donor ligand such as phenyl anion the electron configuration of the metal is d(4)S=1 Fe(IV) coordinated to a (corrolate)(3-) anion, while with one weaker donor ligand such as chloride or other halide, the electron configuration is d(5)S=3/2 Fe(III) coordinated to a (corrolate)(2-.) pi-cation radical, with antiferromagnetic coupling between the metal and corrolate radical electron. Many of these complexes have been studied by electrochemical techniques and have rich redox reactivity, in most cases involving two 1-electron oxidations and two 1-electron reductions, and it is not possible to tell, from the shapes of cyclic voltammetric waves, whether the electron is added or removed from the metal or the macrocycle; often infrared, UV-Vis, or EPR spectroscopy can provide this information. (1)H and (13)C NMR spectroscopic methods are most useful in delineating the spin state and pattern of spin density distribution of the complexes listed above, as would also be expected to be the case for the recently-reported formal Fe(V)O corrolate, if this complex were stable enough for characterization by NMR spectroscopy. Iron, manganese and chromium corrolates can be oxidized by iodosylbenzene and other common oxidants used previously with metalloporphyrinates to effect efficient oxidation of substrates. Whether the "resting state" form of these complexes, most generally in the case of iron [FeCl(Corr)], actually has the electron configuration Fe(IV)(Corr)(3-) or Fe(III)(Corr)(2-.) is not relevant to the high-valent reactivity of the complex. 相似文献
992.
Bud sports are infrequent changes in phenotype affecting shoots of woody perennials but the molecular basis of these mutations has rarely been identified. In this report, we show that the bronze-coloured berries of the Malian cultivar, a documented bud sport of the wine grape Cabernet Sauvignon (Vitis vinifera L.), lack anthocyanins in the subepidermal cells compared to the red/black berried Cabernet Sauvignon in which both the epidermis and several subepidermal cell layers contain anthocyanin. The Malian phenotype is correlated with an alteration in the genome indicated by a reduction of hybridisation signal using a MYBA probe. In Shalistin, a white-berried bud sport of Malian, the red allele at the berry colour locus appears to have been deleted completely. These data suggest that Malian could be a L1/L2 periclinal chimera, which gave rise to Shalistin by an invasion of epidermal cells (L1) by the mutated subepidermal cells (L2). The red grape Pinot Noir has given rise to a number of pale coloured sports, although the provenance of the extant sports is not known. We show that a clone of Pinot Blanc (white-berried) does not have a deletion of the red allele of the same dimensions as that in Shalistin, though a small deletion is a likely explanation for the altered phenotype. However, the mechanism of deletion of the red allele of the berry colour locus is a possible means by which other red to white clonal mutations of grapevines have occurred. 相似文献
993.
994.
Walker K Pakhomova ON Kolb J Schoenbach KS Stuck BE Murphy MR Pakhomov AG 《Bioelectromagnetics》2006,27(3):221-225
The study explored the effect of ambient oxygen on mammalian cell survival after exposure to 10 ns duration, high voltage electrical pulses (nsEP, 80-90 or 120-130 kV/cm; 200-400 pulses per exposure). Cell samples were equilibrated with pure nitrogen, atmospheric air, or pure oxygen prior to the nsEP treatment and were returned to the incubator (air + 5% CO2) shortly after the exposure. The experiments established that survival of hypoxic Jurkat and U937 cells exceeded that of air-equilibrated controls about twofold (P < .01). Conversely, saturation of the medium with oxygen prior to exposure decreased Jurkat cell survival about 1.5 times, P < .01. Attenuation of the cytotoxic effect under hypoxic conditions resembled a well-known effect of oxygen on cell killing by sparsely ionizing radiations and may be indicative of the similarity of underlying cell damage mechanisms. 相似文献
995.
Sophie K. Walker 《Mycologist》2006,20(1):26-31
The cytoskeletal protein actin is among the most abundant proteins in nature. It is almost ubiquitous, occurring in all eukaryotes and in an ancestral form in prokaryotes. Actin monomers can polymerise to form microfilaments, structures that play a critical role in a number of fundamental cell processes in fungi such as morphogenesis, cytokinesis and the movement of organelles. Microfilaments are extremely dynamic structures and can be rapidly modified through their interactions with a number of actin binding proteins (ABPs). The purpose of the following review is to introduce actin and microfilaments in fungi to a general mycological audience and to provide a basic framework from which further study is possible. 相似文献
996.
Novel role for the C terminus of Saccharomyces cerevisiae Rev1 in mediating protein-protein interactions 下载免费PDF全文
The Saccharomyces cerevisiae REV3/7-encoded polymerase zeta and Rev1 are central to the replicative bypass of DNA lesions, a process called translesion synthesis (TLS). While yeast polymerase zeta extends from distorted DNA structures, Rev1 predominantly incorporates C residues from across a template G and a variety of DNA lesions. Intriguingly, Rev1 catalytic activity does not appear to be required for TLS. Instead, yeast Rev1 is thought to participate in TLS by facilitating protein-protein interactions via an N-terminal BRCT motif. In addition, higher eukaryotic homologs of Rev1 possess a C terminus that interacts with other TLS polymerases. Due to a lack of sequence similarity, the yeast Rev1 C-terminal region, located after the polymerase domain, had initially been thought not to play a role in TLS. Here, we report that elevated levels of the yeast Rev1 C terminus confer a strong dominant-negative effect on viability and induced mutagenesis after DNA damage, highlighting the crucial role that the C terminus plays in DNA damage tolerance. We show that this phenotype requires REV7 and, using immunoprecipitations from crude extracts, demonstrate that, in addition to the polymerase-associated domain, the extreme Rev1 C terminus and the BRCT region of Rev1 mediate interactions with Rev7. 相似文献
997.
Robert R. Jackson Mark W. Walker Simon D. Pollard Fiona R. Cross 《Journal of Ethology》2006,24(3):231-238
An important prediction from game theory is that resource value influences the level to which competitors will escalate conflict. An earlier study considered whether this prediction applies to the male–male interactions of Hypoblemum albovittatum, a jumping spider (Salticidae) from New Zealand. The males of this species escalated conflicts in the presence of a moving mount made from a conspecific female. However, because the control was only a similar-sized motionless cork, an alternative hypothesis (that the cue for escalation is seeing movement of any female-size object, rather than seeing specifically a female) was not ruled out. Here we show that a moving cork, without a mount present, is indeed sufficient to cause males to escalate, but a moving mount (made from a conspecific female) causes males to escalate further. The level of escalation in the presence of a moving mount made from prey (housefly) or from a rival (conspecific male) did not differ significantly from the level of escalation when only a moving cork was present. These findings suggest that, although seeing a moving object similar in size to a conspecific female is sufficient for priming males to escalate, males can also discern by sight that specifically a female is present and, when they have this precise information, they make strategic decisions to escalate conflict further. 相似文献
998.
Colson A Brooke AM Walker D Besser GM Chew SL Grossman AB Jenkins PJ Drake WM Monson JP 《Hormone research》2006,66(6):257-267
BACKGROUND/AIMS: This study was designed to determine whether previous Cushing's disease (CD) or prolactinoma (PRL) could exert adverse effects additional to those of growth hormone (GH) deficiency as a consequence of variable degrees of prior hypogonadism or hypercatabolism. We report the effects of 5 years GH treatment in 124 GH deficiency adults; 42 patients with non-functioning pituitary adenomas (NFPA), 43 with treated PRL and 39 with treated CD. METHODS: Fasting plasma glucose, HbA(1c), lipoprotein profile, anthropometry and bone mineral density (BMD) were measured at baseline, 6 months and annually up to 5 years. RESULTS: Mean body mass index remained unchanged in the PRL group and tended to increase in the NFPA group. In contrast, body mass index decreased in the CD group. Decreases in waist and waist/hip ratio were seen in all groups at 6 months. Decreases in total cholesterol and low-density lipoprotein cholesterol were seen in all groups and remained sustained at 5 years. Plasma glucose and HbA(1c) increased at 6 months. Subsequently, plasma glucose returned to baseline values at 5 years; in contrast, HbA(1c )remained unchanged at the end of the study. Baseline lumbar spine and hip BMD were lower in the PRL and CD groups than in the NFPA group, decreased over 1 year in all groups and subsequently increased by 2 years in NFPA with a subsequent increase in lumbar spine BMD in PRL and CD groups delayed to 3-5 years. CONCLUSIONS: Baseline characteristics and response to GH replacement are qualitatively similar in NFPA, PRL and CD patients. Because improvements in BMD occur later in PRL and CD patients, an extended trial of GH therapy may be indicated in those patients who were commenced on GH therapy as an additional treatment for reduced BMD. 相似文献
999.
1000.
Soil Carbon, Nitrogen and Phosphorus Dynamics as Affected by Solarization Alone or Combined with Organic Amendment 总被引:1,自引:0,他引:1
Antonio Gelsomino Luigi Badalucco Loretta Landi Giovanni Cacco 《Plant and Soil》2006,279(1-2):307-325
Soil solarization, alone or combined with organic amendment, is an increasingly attractive approach for managing soil-borne
plant pathogens in agricultural soils. Even though it consists in a relatively mild heating treatment, the increased soil
temperature may strongly affect soil microbial processes and nutrients dynamics. This study aimed to investigate the impact
of solarization, either with or without addition of farmyard manure, in soil dynamics of various C, N and P pools. Changes
in total C, N and P contents and in some functionally-related labile pools (soil microbial biomass C and N, K2SO4-extractable C and N, basal respiration, KCl-exchangeable ammonium and nitrate, and water-soluble P) were followed across
a 72-day field soil solarization experiment carried out during a summer period on a clay loam soil in Southern Italy. Soil
physico-chemical properties (temperature, moisture content and pH) were also monitored. The average soil temperature at 8-cm
depth in solarized soils approached 55 °C as compared to 35 °C found in nonsolarized soil. Two-way ANOVA (solarization×organic
amendment) showed that both factors significantly affected most of the above variables, being the highest influence exerted
by the organic amendment. With no manure addition, solarization did not significantly affect soil total C, N and P pools.
Whereas soil pH, microbial biomass and, at a greater extent, K2SO4-extractable N and KCl-exchangeable ammonium were greatly affected. An increased release of water-soluble P was also found
in solarized soils. Yet, solarization altered the quality of soluble organic residues released in soil as it lowered the C-to-N
ratio of both soil microbial biomass and K2SO4-extractable organic substrates. Additionally, in solarized soils the metabolic quotient (qCO2) significantly increased while the microbial biomass C-to-total organic C ratio (microbial quotient) decreased over the whole
time course. We argued that soil solarization promoted the mineralization of readily decomposable pools of the native soil
organic matter (e.g. the microbial biomass) thus rendering larger, at least over a short-term, the available fraction of some
soil mineral nutrients, namely N and P forms. However, over a longer prospective solarization may lead to an over-exploitation
of labile organic resources in agricultural soils. Manure addition greatly increased the levels of both total and labile C,
N and P pools. Thus, addition of organic amendments could represent an important strategy to protect agricultural lands from
excessive soil resources exploitation and to maintain soil fertility while enhancing pest control. 相似文献