The Role of Adiponectin in Breast Cancer: A Meta-Analysis

Published results suggests that high adiponectin level may decrease the risk of breast cancer. However, available evidence on breast cancer is conflicting. Therefore a meta-analysis was performed to assess the association between blood adiponectin and breast cancer risk. PubMed database, Web of Science, Elsevier Science, Springer Link and bibliographies of retrieved articles were searched for epidemiological studies published up to March 2013. Meta-analysis was performed on the combined effect values (OR) as well as standardized mean difference (SMD) including 17 studies. Fixed or random effect pooled measure was selected on the basis of homogeneity test among studies. The publication bias was assessed by the Egger’s regression asymmetry test and Begg’s rank correlation test with Begg’s funnel plot. Subgroup analyses and sensitivity analysis were also performed. A total of 13 studies involving 3578 breast cancer cases and 4363 controls contributed to the OR analysis. The high adiponectin level did not significantly affect breast cancer risk (OR=0.902, 95% CI=0.773–1.053). After excluding articles that were the key contributors to between-study heterogeneity, the OR of high adiponectin level was associated with decreased breast cancer risk (OR=0.838, 95% CI=0.744–0.943). There was a significantly association between high adiponectin level and postmenopausal breast cancer women (OR=0.752, 95%CI=0.604-0.936); and it was not associated with premenopausal breast cancer women (OR=0.895, 95%CI=0.638-1.256). The result of pooled measure on SMD was that the high adiponectin level was associated with decreased breast cancer risk (SMD= -0.348, 95% CI= -0.533--0.614) after excluding articles which were the key contributors to between-study heterogeneity. Our findings indicate that high adiponectin level might decrease the risk of postmenopausal breast cancer. More randomized clinical trials and observational studies are needed to confirm this association with underlying biological mechanisms in the future.     

关于《中国植物志》中鹿药(百合科)花特征的纠正

根据野外调查,同时结合文献分析,指出《中国植物志》和Flora of China中关于百合科(Liliaceae)鹿药花部特征描述存在的问题,并提出修改建议。此外,对其学名进行了简要讨论。     

六妹羊肚菌的化学成分

以六妹羊肚菌Morchella sextelata为研究对象,对其子实体的化学成分进行研究。采用气相色谱-质谱联用技术（GC-MS）进行子实体芳香物和亲脂性提取物的化学成分分析,同时对其亲脂性提取物的抗氧化和抗菌活性进行了初步评价;采用正反相硅胶柱色谱、葡聚糖凝胶柱色谱等多种色谱分离方法进行化学成分的分离纯化,并通过核磁共振（NMR）、质谱（MS）等技术鉴定化合物结构。从六妹羊肚菌子实体的芳香物中共鉴定出26个化合物,辛-1-烯-3-醇（32.53%）、(E)-辛-2-烯醛（25.15%）和苯乙醛（12.31%）为主要成分;从六妹羊肚菌子实体的亲脂性提取物中共鉴定出14个化合物,亚油酸（77.80%）为主要成分;六妹羊肚菌亲脂性提取物仅显示出中等强度的抗氧化活性。从六妹羊肚菌子实体中共分离鉴定出14个化合物,包括7个甾体类化合物,其中化合物1、2、4、7、11、13和14为首次从羊肚菌属中分离得到。本研究首次对六妹羊肚菌的小分子化学成分进行了分析,对羊肚菌活性物质的阐明及进一步开发利用具有重要意义。     

美国白蛾天敌研究进展

美国白蛾Hyphantria cunea(Drury)是国家林业和草原局的重点防控对象,也是我国重大外来林业检疫性有害生物.现阶段美国白蛾在我国的种群密度持续增加和扩散,缺乏有效天敌的控制应该是重要的原因之一.本文综述了国内、外美国白蛾的捕食性和寄生性天敌的种类.捕食性天敌主要包括昆虫、蜘蛛、两栖类和鸟类,整理出国外报道的捕食性天敌名录119种,国内捕食性天敌名录29种;寄生性天敌主要包括寄生蜂和寄生蝇类,整理出国外报道的寄生性天敌名录47种,国内寄生性天敌名录53种.本文回顾了我国在美国白蛾天敌利用方面取得的阶段成果,并针对将来天敌复合体的应用和原产地天敌的引进提出了展望.     

白云鄂博稀土矿区土壤中放线菌特征调查研究

利用稀释平板涂布法对白云鄂博稀土矿区土壤中放线菌进行了分离与形态观察,同时应用相似性指数、丰富度指数、多样性指数以及均匀度指数等生态学方法对该地区放线菌的生态学特征进行了分析,并在此基础上分别对所选放线菌的耐盐性、耐酸碱性、耐低温或高温以及耐高辐射性元素等耐性指标进行了调查研究.结果表明:①经纯培养及菌落颜色和形态观察...     

Caudatin inhibits carcinomic human alveolar basal epithelial cell growth and angiogenesis through modulating GSK3β/β-catenin pathway

In this study, we investigate the anti‐cancer activity of caudatin in carcinomic human alveolar basal epithelial cell line A549 and anti‐angiogenic activity in human umbilical vein endothelial cells (HUVECs). We show that caudatin impairs the cell viability and induces G₀/G₁ phase arrest in A549 cells with a dose dependent manner. A549 cells, not HUVECs, dealing with caudatin exhibited typical characteristics of apoptosis, which were accompanied by activation of caspase‐3, caspase‐9 and Poly(ADP–Ribose) Polymerase (PARP). In addition, caudatin treatment resulted in a decrease of β‐catenin and increase of phosphorylation of β‐catenin, and inhibited phosphorylation levels of GSK3β (Ser 9) in A549 cells. Conditional medium of A549 cells‐induced or growth factors‐induced tube formation of HUVECs was markedly inhibited by caudatin treatment, which was associated with the inhibiting VEGF secretion from A549 cells by caudatin. Our findings suggest that caudatin inhibits carcinomic human alveolar basal epithelial cell growth and angiogenesis by targeting GSK3β/β‐catenin pathway and suppressing VEGF production. J. Cell. Biochem. 113: 3403–3410, 2012. © 2012 Wiley Periodicals, Inc.     

Y染色体单倍群与西南地区男性生精障碍的相关性

男性不育中, 原发无精、少精是最为重要的因素之一, 核型异常和无精子症因子(Azoospermia factor, AZF)微缺失能解释部分原发无精、少精的原因, 然而还有许多致病因素尚不清楚。Y染色体作为男性特有的染色体, 与男性生殖系统的正常功能密切相关。文章主要对Y染色体单倍群这一分子遗传背景与男性原发无精、严重少精症之间是否存在相关性进行探讨, 为进一步探索原发无精、严重少精症的遗传学致病原因提供依据和可行的方向。采集265名生精障碍患者(原发无精症患者193名, 原发严重少精症患者72名)以及193名正常男性样本的外周血, 进行核型分析和AZF缺失分析, 以排除有此两类异常的样本。将经过筛选的样本进行Y染色体单倍群分析, 并对其单倍群分布情况进行统计分析。分析显示, 生精障碍组和对照组分别在D1*、F*、K*、N1*和O3* 上有显著性差异(P=0.032, 0.022, 0.009, 0.009, 0.017, <0.05)。Y染色体单倍群, 这一Y染色体遗传背景与男性原发生精障碍的发生有相关性。     

Synthesis,biological activities and SAR studies of new 3-substitutedphenyl-4-substitutedbenzylideneamino-1,2,4-triazole Mannich bases and bis-Mannich bases as ketol-acid reductoisomerase inhibitors

A series of new 3-substitutedphenyl-4-substitutedbenzylideneamino-1,2,4-triazole Mannich bases and bis-Mannich bases were synthesized through Mannich reaction with high yields. Their structures were confirmed by means of IR, ¹H NMR, ¹³C NMR and elemental analysis. The preliminary bioassay indicated that compounds 7g, 7h and 7l exhibited potent in vitro inhibitory activities against ketol-acid reductoisomerase (KARI) with K_i value of (0.38?±?0.25), (6.59?±?2.75) and (8.46?±?3.99)?μmol/L, respectively, and were comparable with IpOHA. They could be new KARI inhibitors for follow-up research. Some of the title compounds also exhibited obvious herbicidal activities against Echinochloa crusgalli and remarkable in vitro fungicidal activities against Physalospora piricola and Rhizoctonia cerealis. The SAR of the compounds were analyzed, in which the molecular docking revealed the binding mode of 7g with the KARI, and the 3D-QSAR results provided useful information for guiding further optimization of this kind of structures to discover new fungicidal agents towards Rhizoctonia cerealis.     

FHL2通过相互作用抑制分化抑制蛋白家族成员的转录抑制活性

目的：探讨FHL2与Id（分化抑制蛋白）家族蛋白之间的相互作用及FHL2对Id蛋白功能的调控效应。方法：用GST-pulldown与免疫共沉淀（CoIP）方法检测FHL2与Id家族蛋白成员之间在体内外的相互作用;用共转染与报告基因驱动的萤光素酶方法检测FHL2对Id蛋白介导转录抑制效应的调控作用。结果：FHL2与Id家族的4个蛋白均存在直接的相互作用关系,表位分析结果显示FHL2蛋白中的第2个LIM结构域在FHL2/Id相互作用中是必需的,Id蛋白N端结构域在介导FHL2/Id相互作用中是必需的,FHL2/Id相互作用不依赖于Id蛋白中的螺旋-环-螺旋结构;通过相互作用,FHL2阻止了Id蛋白对碱性螺旋-环-螺旋转录因子E47转录活性的抑制作用。结论：FHL2是一个新识别的Id蛋白广谱的相互作用因子,通过对Id蛋白功能活性的抑制效应,FHL2可能参与Id介导的多种生物学效应以及肿瘤发生与进展。